Isotherm for Adsorption of Agrobacterium tumefaciens to Susceptible Potato (Solanum tuberosum L.) Tissues †

Agrobacterium tumefaciens Mediated Genetic Transformation of acvB Gene in Potato (Solanum tuberosum L.). Genetic transformations are now routinely applied to plant mediated by Agrobacterium tumefaciens as the most convenient technique. This study aimed to prove the success of A. tumefaciens mediated genetic transformation in potato. A. tumefaciens LBA (pBI 121) and explant of potato shoot were used in this study. Explants were grown in vitro on Murashige and Skoog media. Transformation was implemented using smear technique by smearing A. tumefaciens to injured explant. Experimental groups consisted of two groups: control group which did not receive transformation treatment and treatment group receiving transformation treatment. Explant growth was observed through the presence of shoots, branches and the shoot height. Explants in the treatment group resulted in a higher number of shoots, branches, and shoot heights compared to control. Phenol compounds appear in explant epidermal tissue, indicating the wounds produced by A. tumefaciens infection, thus the gene predicted to be transformed. Identification by PCR is needed to prove the existence of the acvB gene in potato plants genome, using acvB specific PCR primer as the marker, such as (5?-CCCT CTAG AGAC CCGC GCCA AGGCG-3?) and (5?CGCG TCGA CCTT GTCG GAAAG -3?) with 540-bp in base pair size produced.

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Pengaruh Jenis Bakteri Pelarut terhadap Pertumbuhan dan Hasil Benih G3 Kentang (Solanum tuberosum L.) Varietas Medians.

Paspalum: Jurnal Ilmiah Pertanian ◽

10.35138/paspalum.v8i2.200 ◽

2020 ◽

Vol 8 (2) ◽

pp. 131

Author(s):

Indriana Ulfah ◽

Linlin Parlinah ◽

Noertjahyani Noertjahyani ◽

Rohana Abdullah

Keyword(s):

Solanum Tuberosum ◽

Agrobacterium Tumefaciens ◽

Solanum Tuberosum L ◽

Potato Crop ◽

Growth And Yield ◽

Phosphate Solubilizing Bacteria ◽

Group Design ◽

Number Of Leaves ◽

Phosphate Solubilizing ◽

Experimental Garden

This article aims to examine the effect of phosphate solubilizing bacteria (PSB) on the growth and yield of G3 seeds of potato (solanum tuberosum L.) varieties of medians. The experiment was conducted in the experimental garden of The Faculty of Agriculture of Winaya Mukti University Tanjungsari, Sumedang with a height of 850 meters above sea level with the order of land Andisol. Time trials are conducted from May to August 2019. The design used is a simple RandomIzed Group Design consisting of five types of phosphate Solubilizing bacteria, namely without PSB, Pseudacidovorax intermedius, Agrobacterium fabrum, Agrobacterium tumefaciens, Serratia nematodiphila and repeated five times each. The results of the experiment showed that the administration of this type of PSB was able to increase the height of the plant and the number of leaves at the age of 28 DAP and 42 DAP and give the result of the number of potatoes and the weight of the perpetak tubers on the potato crop of the medians varieties better.

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Gamma Radiation Effect on Agrobacterium tumefaciens-Mediated Gene Transfer in Potato (Solanum tuberosum L.)

10.5772/intechopen.99878 ◽

2021 ◽

Author(s):

Murat Aycan ◽

Muhammet Cagri Oguz ◽

Yasin Ozgen ◽

Burak Onol ◽

Mustafa Yildiz

Keyword(s):

Solanum Tuberosum ◽

Agrobacterium Tumefaciens ◽

Transgenic Plants ◽

Gamma Radiation ◽

Solanum Tuberosum L ◽

Pcr Analysis ◽

Axillary Meristems ◽

Technological Research Council ◽

Selection Medium ◽

Transformation Methods

Potato (Solanum tuberosum L.) is one of the major crops of the world. Significant improvements can be achieved in terms of yield and quality by the determination of efficient transformation methods. On the other hand, low transformation frequency seriously limits the application of molecular techniques in obtaining transgenic crops. In the present study, the effect of gamma radiation on Agrobacterium tumefaciens-mediated transformation to the potato was firstly investigated. Sterile seedlings of potato cv. ‘Marabel’, which was grown on Gamborg’s B5 medium in Magenta vessels, were irradiated with different gamma radiation doses (0-control, 40, 80, 120 Gy 60Co). Stem parts having axillary meristems were excised from irradiated seedlings and inoculated by A. tumefaciens (GV2260), which harbors the binary plasmid p35S GUS-INT contains and GUS (β-glucuronidase) gene controlled by 35S promoter (CaMV) and nptII (neomycin phosphotransferase II) gene driven by NOS (nopaline synthase) promoter). Inoculated stem parts having axillary meristems explants were then directly transported to a selection medium containing duocid (500 mg l−1), and kanamycin (100 mg l−1), 4 mg l−1 gibberellic acid, 1 mg l−1 BAP and 0.1 mg l−1 NAA. The adult transgenic plants were detected by polymerase chain reaction (PCR) analysis. According to the number of transgenic plants determined by PCR analysis, results obtained from explants treated with 40 Gy gamma gave the best results compared to the control (0 Gy) application. The doses over 40 Gy were also found statistically significant compared to the control (0 Gy). It is expected that the protocol described in this study make the transformation studies easier by skipping the stages of ‘co-cultivation’, ‘culturing explants on selection medium’ and ‘recovery of transgenic shoots on selection medium’ not only for potato but also for other crop plants. This study was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBİTAK) (Grant number 113O280 to Prof. Dr. Mustafa YILDIZ).

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Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I

Biopolymers and Cell ◽

10.7124/bc.000420 ◽

1996 ◽

Vol 12 (2) ◽

pp. 42-46 ◽

Cited By ~ 1

Author(s):

P. G. Kovalenko ◽

I. M. Yefimenko ◽

N. V. Schuman ◽

T. V. Medvedeva ◽

K. G. Gazaryan ◽

...

Keyword(s):

Solanum Tuberosum ◽

Agrobacterium Tumefaciens ◽

Genetic Transformation ◽

Solanum Tuberosum L ◽

Class I ◽

Patatin Promoter ◽

Promoter Class

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Heat shock enhanced Agrobacterium tumefaciens mediated T-DNA delivery to potato (Solanum tuberosum L.)

Journal of Plant Biochemistry and Biotechnology ◽

10.1007/s13562-021-00762-1 ◽

2022 ◽

Author(s):

Amanpreet Kaur ◽

M. Sudhakara Reddy ◽

Anil Kumar

Keyword(s):

Solanum Tuberosum ◽

Agrobacterium Tumefaciens ◽

Heat Shock ◽

Solanum Tuberosum L ◽

Dna Delivery

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Transformação genética da batata cultivar Achat via Agrobacterium tumefaciens

Horticultura Brasileira ◽

10.1590/s0102-05362000000100009 ◽

2000 ◽

Vol 18 (1) ◽

pp. 41-45 ◽

Cited By ~ 1

Author(s):

Antonio Carlos Torres ◽

Adriana Teixeira Ferreira ◽

Eduardo Romano ◽

Mônica Kangussú Cattony ◽

Adriana Souza Nascimento

Keyword(s):

Solanum Tuberosum ◽

Agrobacterium Tumefaciens ◽

Southern Blotting ◽

Solanum Tuberosum L ◽

Npt Ii

Explantes de segmentos nodais de batata (Solanum tuberosum L.) cultivar Achat provenientes da cultura in vitro foram transformados, via Agrobacterium tumefaciens, estirpe LBA4404, contendo o vetor binário pBI121 com os genes npt II e o gus-intron. A regeneração de potenciais transformantes foi feita em meio seletivo contendo 50 mg.L-1 de canamicina. Foram obtidas plantas expressando resistência à canamicina e atividade da b-glucuronidase. Pela análise molecular nas plantas gus+, via PCR, constatou-se a amplificação dos genes npt II. A hibridização por Southern blotting demonstrou a inserção de pelo menos uma cópia do gene introduzido.

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Situación económica y social de la población de San José del Carmen, San Cristóbal de las Casas, Chiapas

RECUBRIMIENTO COMESTIBLE CON BASE DE PIÑON MEXICANO (Jatropha curcas) SOBRE LA CALIDAD DE CHAYOTE MINIMAMENTE PROCESADO ◽

10.32854/agrop.v0i0.1371 ◽

2019 ◽

Vol 12 (4) ◽

Author(s):

Pedro Cadena-Iñiguez ◽

Eileen Salinas-Cruz ◽

Jesús Martínez-Sánchez ◽

Mariano Morales-Guerra ◽

Romualdo Vásquez-Ortiz ◽

...

Keyword(s):

Solanum Tuberosum ◽

Theobroma Cacao ◽

Zea Mays L ◽

Solanum Tuberosum L ◽

San Jose ◽

Phaseolus Vulgaris L ◽

Las Casas ◽

Vicia Faba L ◽

Las Mujeres ◽

Theobroma Cacao L

Objetivo: Establecer una línea base como fundamento para la intervención e inducción de innovaciones a través de escuelas de campo y planes de negocios. Diseño/metodología/aproximación: El estudio se desarrolló en San José del Carmen, San Cristóbal de Las Casas, Chiapas, México. Se elaboró una encuesta como instrumento para recabar en campo información de familias de la localidad con la finalidad de conocer su situación actual. La localidad de trabajo se eligió utilizando como criterio las poblaciones que se encuentran dentro de Cruzada Nacional contra el hambre y en la clasificación de la pobreza extrema Resultados: La localidad de San José del Carmen es considerada como de alta marginación, la población es bilingüe, su principal lengua es el Tzotzil, el nivel de estudios promedio es hasta el tercer año de primaria, las principales actividades productivas son la siembra de maíz (Zea mays L.), frijol (Phaseolus vulgaris L.), papa (Solanum tuberosum L.) y haba (Vicia faba L.) que son utilizadas principalmente para autoconsumo. No cuentan con agua potable ya que su sistema es a través de la recaudación de agua de lluvia o a través de un jagüey. Sus principales actividades económicas son la albañilería por parte de los hombres, y venta de productos, tales como el pozol (bebida energética a base de maíz y cacao (Theobroma cacao L.), tostadas y algunos vegetales por parte de las mujeres. Las tostadas son producidas en forma artesanal con un proceso de doble nixtamalización y vendida en bolsas de 20 tostadas cada una, a un costo de MX$10.00 en los mercados de San Cristóbal de las Casas, una a dos veces por semana alrededor de 40 bolsas por día de venta. Limitaciones del estudio/implicaciones: La afiliación zapatista de alguno de los pobladores y el hecho de que algunos habitantes no hablen español, fueron factores que limitaron desarrollar al 100% el estudio; sin embargo, se considera que se tienen los elementos necesarios para establecer la línea base de la situación de San José del Carmen. Conclusiones: El diagnóstico servirá como una herramienta para establecer una intervención a través de innovaciones y planes de negocios en la comunidad de estudio. Los pobladores cuentan con herramientas necesarias para obtener nuevos conocimientos que ayuden a un desarrollo de su producción. El idioma no deberá de ser una limitante para ello.

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