Heat shock enhanced Agrobacterium tumefaciens mediated T-DNA delivery to potato (Solanum tuberosum L.)

2022 ◽  
Author(s):  
Amanpreet Kaur ◽  
M. Sudhakara Reddy ◽  
Anil Kumar
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Heat Shock ◽  
Solanum Tuberosum L ◽  
Dna Delivery

scholarly journals Transformasi Genetik Tanaman Kentang (Solanum tuberosum L.) dengan Gen acvB Menggunakan Vektor Agrobacterium tumefaciens

2021 ◽  
Vol 11 (1) ◽  
pp. 63
Author(s):  
DARWIN SILALAHI ◽  
I GEDE PUTU WIRAWAN ◽  
MADE SRITAMIN
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Treatment Group ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Control Group ◽  
Shoot Height ◽  
Specific Pcr ◽  
Convenient Technique

Agrobacterium tumefaciens Mediated Genetic Transformation of acvB Gene in Potato (Solanum tuberosum L.). Genetic transformations are now routinely applied to plant mediated by Agrobacterium tumefaciens as the most convenient technique. This study aimed to prove the success of A. tumefaciens mediated genetic transformation in potato. A. tumefaciens LBA (pBI 121) and explant of potato shoot were used in this study. Explants were grown in vitro on Murashige and Skoog media. Transformation was implemented using smear technique by smearing A. tumefaciens to injured explant. Experimental groups consisted of two groups: control group which did not receive transformation treatment and treatment group receiving transformation treatment. Explant growth was observed through the presence of shoots, branches and the shoot height. Explants in the treatment group resulted in a higher number of shoots, branches, and shoot heights compared to control. Phenol compounds appear in explant epidermal tissue, indicating the wounds produced by A. tumefaciens infection, thus the gene predicted to be transformed. Identification by PCR is needed to prove the existence of the acvB gene in potato plants genome, using acvB specific PCR primer as the marker, such as (5?-CCCT CTAG AGAC CCGC GCCA AGGCG-3?) and (5?CGCG TCGA CCTT GTCG GAAAG -3?) with 540-bp in base pair size produced.

scholarly journals Introduction of Arabidopsis’s heat shock factor HsfA1d mitigates adverse effects of heat stress on potato (Solanum tuberosum L.) plant

2020 ◽  
Vol 25 (1) ◽  
pp. 57-63
Author(s):  
Zamarud Shah ◽  
Safdar Hussain Shah ◽  
Gul Shad Ali ◽  
Iqbal Munir ◽  
Raham Sher Khan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Heat Shock ◽  
Adverse Effects ◽  
Solanum Tuberosum L ◽  
Heat Shock Factor

scholarly journals Pengaruh Jenis Bakteri Pelarut terhadap Pertumbuhan dan Hasil Benih G3 Kentang (Solanum tuberosum L.) Varietas Medians.

2020 ◽  
Vol 8 (2) ◽  
pp. 131
Author(s):  
Indriana Ulfah ◽  
Linlin Parlinah ◽  
Noertjahyani Noertjahyani ◽  
Rohana Abdullah
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Solanum Tuberosum L ◽  
Potato Crop ◽  
Growth And Yield ◽  
Phosphate Solubilizing Bacteria ◽  
Group Design ◽  
Number Of Leaves ◽  
Phosphate Solubilizing ◽  
Experimental Garden

This article aims to examine the effect of phosphate solubilizing bacteria (PSB) on the growth and yield of G3 seeds of potato (solanum tuberosum L.) varieties of medians. The experiment was conducted in the experimental garden of The Faculty of Agriculture of Winaya Mukti University Tanjungsari, Sumedang with a height of 850 meters above sea level with the order of land Andisol. Time trials are conducted from May to August 2019. The design used is a simple RandomIzed Group Design consisting of five types of phosphate Solubilizing bacteria, namely without PSB, Pseudacidovorax intermedius, Agrobacterium fabrum, Agrobacterium tumefaciens, Serratia nematodiphila and repeated five times each. The results of the experiment showed that the administration of this type of PSB was able to increase the height of the plant and the number of leaves at the age of 28 DAP and 42 DAP and give the result of the number of potatoes and the weight of the perpetak tubers on the potato crop of the medians varieties better.

scholarly journals Gamma Radiation Effect on Agrobacterium tumefaciens-Mediated Gene Transfer in Potato (Solanum tuberosum L.)

2021 ◽  
Author(s):  
Murat Aycan ◽  
Muhammet Cagri Oguz ◽  
Yasin Ozgen ◽  
Burak Onol ◽  
Mustafa Yildiz
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Gamma Radiation ◽  
Solanum Tuberosum L ◽  
Pcr Analysis ◽  
Axillary Meristems ◽  
Technological Research Council ◽  
Selection Medium ◽  
Transformation Methods

Potato (Solanum tuberosum L.) is one of the major crops of the world. Significant improvements can be achieved in terms of yield and quality by the determination of efficient transformation methods. On the other hand, low transformation frequency seriously limits the application of molecular techniques in obtaining transgenic crops. In the present study, the effect of gamma radiation on Agrobacterium tumefaciens-mediated transformation to the potato was firstly investigated. Sterile seedlings of potato cv. ‘Marabel’, which was grown on Gamborg’s B5 medium in Magenta vessels, were irradiated with different gamma radiation doses (0-control, 40, 80, 120 Gy 60Co). Stem parts having axillary meristems were excised from irradiated seedlings and inoculated by A. tumefaciens (GV2260), which harbors the binary plasmid p35S GUS-INT contains and GUS (β-glucuronidase) gene controlled by 35S promoter (CaMV) and nptII (neomycin phosphotransferase II) gene driven by NOS (nopaline synthase) promoter). Inoculated stem parts having axillary meristems explants were then directly transported to a selection medium containing duocid (500 mg l−1), and kanamycin (100 mg l−1), 4 mg l−1 gibberellic acid, 1 mg l−1 BAP and 0.1 mg l−1 NAA. The adult transgenic plants were detected by polymerase chain reaction (PCR) analysis. According to the number of transgenic plants determined by PCR analysis, results obtained from explants treated with 40 Gy gamma gave the best results compared to the control (0 Gy) application. The doses over 40 Gy were also found statistically significant compared to the control (0 Gy). It is expected that the protocol described in this study make the transformation studies easier by skipping the stages of ‘co-cultivation’, ‘culturing explants on selection medium’ and ‘recovery of transgenic shoots on selection medium’ not only for potato but also for other crop plants. This study was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBİTAK) (Grant number 113O280 to Prof. Dr. Mustafa YILDIZ).

scholarly journals Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)

2012 ◽  
Vol 64 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Jelena Savic ◽  
Ivana Dragicevic ◽  
D. Pantelic ◽  
Jasmina Oljaca ◽  
Ivana Momcilovic
Keyword(s):  
Heat Stress ◽  
Solanum Tuberosum ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Solanum Tuberosum L ◽  
Immunoblot Analysis ◽  
Control Treatment ◽  
Ex Vitro ◽  
Heat Tolerant

We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (?Laura?), moderately sensitive (?Liseta?) and heat-sensitive (?Agria?) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ?Laura? accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ?Liseta? and ?Agria?. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.

scholarly journals Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I

1996 ◽  
Vol 12 (2) ◽  
pp. 42-46 ◽  
Author(s):  
P. G. Kovalenko ◽  
I. M. Yefimenko ◽  
N. V. Schuman ◽  
T. V. Medvedeva ◽  
K. G. Gazaryan ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Solanum Tuberosum L ◽  
Class I ◽  
Patatin Promoter ◽  
Promoter Class
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