scholarly journals Isolation and Characterization of a New Denitrifying Spirillum Capable of Anaerobic Degradation of Phenol

2000 ◽  
Vol 66 (4) ◽  
pp. 1286-1291 ◽  
Author(s):  
Yoshifumi Shinoda ◽  
Yasuyoshi Sakai ◽  
Makiko Ué ◽  
Akira Hiraishi ◽  
Nobuo Kato
Keyword(s):  
Dna Sequences ◽  
Enrichment Culture ◽  
Aerobic Growth ◽  
Ferrous Ions ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Enrichment Process ◽  
Better Than

ABSTRACT Two kinds of phenol-degrading denitrifying bacteria,Azoarcus sp. strain CC-11 and spiral bacterial strain CC-26, were isolated from the same enrichment culture after 1 and 3 years of incubation, respectively. Both strains required ferrous ions for growth, but strain CC-26 grew better than strain CC-11 grew under iron-limited conditions, which may have resulted in the observed change in the phenol-degrading bacteria during the enrichment process. Strain CC-26 grew on phenol, benzoate, and other aromatic compounds under denitrifying conditions. Phylogenetic analysis of 16S ribosomal DNA sequences revealed that this strain is most closely related to aMagnetospirillum sp., a member of the α subclass of the class Proteobacteria, and is the first strain of a denitrifying aromatic compound-degrading bacterium belonging to this group. Unlike previously described Magnetospirillumstrains, however, this strain did not exhibit magnetotaxis. It grew on phenol only under denitrifying conditions. Other substrates, such as acetate, supported aerobic growth, and the strain exhibited microaerophilic features.

scholarly journals Isolation and Characterization of Microcystin Degrading Bacteria from Holy Ponds in India

2017 ◽  
Vol 4 (4) ◽  
pp. 436-447 ◽  
Author(s):  
Vikram Pal Gandhi ◽  
Anil Kumar
Keyword(s):  
Restriction Analysis ◽  
Water Bodies ◽  
Rrna Gene ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Chronic Poisoning ◽  
Degrading Bacteria ◽  
Health Related ◽  
Water Treatments

Microcystins (MCs) are toxic cyclic heptapeptides produced by few toxic cyanobacteria and generally form blooms in eutrophic surface fresh water bodies. They cause acute to chronic poisoning and other health related problems mainly by irreversible inhibition of protein phosphatases (PP1 and PP2A) and increased formation of reactive oxygen species (ROS).  Due to limitation of non-biological methods of water treatments the exploration of MCs degrading bacteria is emerging at a quite pace to address, through bioremediation, the problems posed by MCs in water and water-bodies. Report and study of MCs biodegrading bacteria from India were lacking. However it was evident, from our previous study, that microcystin degradation can be achieved by indigenous microcystin degrading bacterial population in its natural place where microcystin producing blooms occur. This study has presented isolation and characterization of indigenous microcystin degrading bacteria from holy ponds in Utter Pradesh of India. Overall 20 bacterial isolates were isolated from Microcystis infested different ponds. Out of these 13 isolates were mlrA positive by PCR and were found to be distinct isolates by amplified ribosomal DNA restriction analysis (ARDRA). However, ARDRA analysis revealed overall four bacterial groups. On the basis of 16S-rRNA gene sequence the Gram-positive-rod isolate PM1 was identified, with 99% identity, as Bacillus licheniformis which was shown earlier to cluster with microcystin degrading bacterium B. subtilis. Thus the present study revealed, for the first time, probable microcystin degrading bacteria in water-bodies from India. The potential and the metabolic pathway of PM1 and other mlrA positive isolates need to be further studied and validated to confirm their application in microcystin bioremediation. Int J Appl Sci Biotechnol, Vol 4(4): 436-447

Isolation and characterization of 3-nitrophenol-degrading bacteria associated with rhizosphere of Spirodela polyrrhiza

2012 ◽  
Vol 19 (5) ◽  
pp. 1852-1858 ◽  
Author(s):  
Risky Ayu Kristanti ◽  
Masahiro Kanbe ◽  
Tony Hadibarata ◽  
Tadashi Toyama ◽  
Yasuhiro Tanaka ◽  
...  
Keyword(s):  
Spirodela Polyrrhiza ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3

2019 ◽  
Vol 51 (2) ◽  
pp. 170-178
Author(s):  
Tao Peng ◽  
Qing-Kun Chen ◽  
Jing-Sheng Lun ◽  
Amit Pratush ◽  
Guang-Ming Xiong ◽  
...  
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant

2018 ◽  
Vol 78 (7) ◽  
pp. 1517-1524 ◽  
Author(s):  
Riqiang Li ◽  
Jianxing Wang ◽  
Hongjiao Li
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Removal Rate ◽  
Treatment Plant ◽  
Coking Wastewater ◽  
Naphthalene Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Abstract As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.

scholarly journals Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

2005 ◽  
Vol 251 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Li Yang ◽  
Yu-hua Zhao ◽  
Bing-xin Zhang ◽  
Ching-Hong Yang ◽  
Xin Zhang
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF PHENOL DEGRADING BACTERIA FROM INDUSTRIAL WASTEWATER AND SEWAGE WATER

2021 ◽  
Vol 48 (2) ◽  
pp. 443-457
Author(s):  
Salma Salem ◽  
H. Abd El‌‌‌-Fattah ◽  
Howaida Abdelbasit ◽  
S. Mahgoub
Keyword(s):  
Industrial Wastewater ◽  
Sewage Water ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and Characterization of a Novel p,p’-DDT-Degrading Bacterium: Aeromonas sp. Strain MY1 from Agricultural Soil

2020 ◽  
pp. 12-22
Author(s):  
Y. Murtala ◽  
B. C. Nwanguma ◽  
L. U. S. Ezeanyika
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Phylogenetic Analyses ◽  
Inhibitory Effect ◽  
Aerobic Conditions ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Background: Despite the banned on the use of dichlorodiphenyltrichloroethane (DDT) and other Persistent Organic Pollutants (POPs) by the Stockholm Convention for their toxicity, emerging shreds of evidence have indicated that DDT is, however, still in use in developing countries. This might increase the global burden of DDT contamination and its hazardous effects. Aim: This study focused on the isolation and characterization of p,p’-DDT-degrading bacterium from a tropical agricultural soil. Methodology: Standard isolation procedure was used for the screening and isolation of the strain. The 16S rRNA and phylogenetic analyses were used to identify the isolate and established protocols were followed to characterize the strain. Results: A new strain belonging to the genus Aeromonas was isolated from agricultural soil using minimal salt-p,p’-DDT enrichment medium. The 16S rRNA sequencing was used to identify the strain and the partial sequence was deposited in the NCBI GenBank as Aeromonas sp. Strain MY1. This mesophilic isolate was capable of utilizing up to 50 mgL-1 of p,p’-DDT as the sole carbon source at an optimum pH of 7.5 and optimum temperature of 35 °C within 120 h under aerobic conditions. Fe2+ (0.2 mgL-1) demonstrated a stimulatory effect on the p,p’-DDT degradation capacity by the strain MY1. However, Zn, Cu, Pb, Hg, Ag and Cr ions have demonstrated various patterns of inhibitory effect on the p,p’-DDT degradation capacity of the isolate at 0.2 mgL-1. The strain MY1 could be a promising candidate for the bioremediation of p,p’-DDT contaminant. Conclusion: Aeromonas sp. strain MY1 was capable of utilizing p,p’-DDT as a sole carbon source under aerobic conditions. The utilization capacity of the strain was influenced by some heavy metals. Fe was found to enhance the p,p’-DDT utilization capacity of the isolate at a lower concentration. While Zn, Cu, Pb, Hg, Ag and Cr showed various patterns of inhibitory effect.

scholarly journals Isolation and Characterization of Hydrocarbon-Degrading Bacteria from Wastewaters in Ouagadougou, Burkina Faso

2014 ◽  
Vol 05 (12) ◽  
pp. 1183-1196 ◽  
Author(s):  
Adama Sawadogo ◽  
Otoidobiga C. Harmonie ◽  
Joseph B. Sawadogo ◽  
Aminata Kaboré ◽  
Alfred S. Traoré ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Isolation And Characterization ◽  
Degrading Bacteria
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