scholarly journals Isolation and Characterization of Microcystin Degrading Bacteria from Holy Ponds in India

2017 ◽  
Vol 4 (4) ◽  
pp. 436-447 ◽  
Author(s):  
Vikram Pal Gandhi ◽  
Anil Kumar
Keyword(s):  
Restriction Analysis ◽  
Water Bodies ◽  
Rrna Gene ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Chronic Poisoning ◽  
Degrading Bacteria ◽  
Health Related ◽  
Water Treatments

Microcystins (MCs) are toxic cyclic heptapeptides produced by few toxic cyanobacteria and generally form blooms in eutrophic surface fresh water bodies. They cause acute to chronic poisoning and other health related problems mainly by irreversible inhibition of protein phosphatases (PP1 and PP2A) and increased formation of reactive oxygen species (ROS).  Due to limitation of non-biological methods of water treatments the exploration of MCs degrading bacteria is emerging at a quite pace to address, through bioremediation, the problems posed by MCs in water and water-bodies. Report and study of MCs biodegrading bacteria from India were lacking. However it was evident, from our previous study, that microcystin degradation can be achieved by indigenous microcystin degrading bacterial population in its natural place where microcystin producing blooms occur. This study has presented isolation and characterization of indigenous microcystin degrading bacteria from holy ponds in Utter Pradesh of India. Overall 20 bacterial isolates were isolated from Microcystis infested different ponds. Out of these 13 isolates were mlrA positive by PCR and were found to be distinct isolates by amplified ribosomal DNA restriction analysis (ARDRA). However, ARDRA analysis revealed overall four bacterial groups. On the basis of 16S-rRNA gene sequence the Gram-positive-rod isolate PM1 was identified, with 99% identity, as Bacillus licheniformis which was shown earlier to cluster with microcystin degrading bacterium B. subtilis. Thus the present study revealed, for the first time, probable microcystin degrading bacteria in water-bodies from India. The potential and the metabolic pathway of PM1 and other mlrA positive isolates need to be further studied and validated to confirm their application in microcystin bioremediation. Int J Appl Sci Biotechnol, Vol 4(4): 436-447

scholarly journals Isolation and characterization of the cellulose-degrading bacteria from Ngoc Linh ginseng soil in Quang Nam province

2016 ◽  
Vol 14 (1) ◽  
pp. 55-61
Author(s):  
Trần Bảo Trâm ◽  
Phạm Hương Sơn ◽  
Ngô Thị Hiền ◽  
Ngô Thị Hoa ◽  
Nguyễn Thu Hiền ◽  
...  
Keyword(s):  
Thick Layer ◽  
Rrna Gene ◽  
Ideal Condition ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Ginseng Soil ◽  
The Ideal

Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) is an endemic species in Vietnam and was discovered at the Ngoc Linh mountain (Kon Tum/Quảng Nam). Investigations showed that the soil with a thick layer of humus was the ideal condition for growth and development of Ngoc Linh ginseng. Therefore research on microbial flora as well as cellulose-degrading bacteria in ginseng soil may elucidate factors contributing to acclimatized cultivation of this ginseng in Vietnam. From the soil sample with cultivated Ngoc Linh ginseng in Quang Nam, five bacteria strains with cellulose-degrading activities were isolated (QN1, QN2, QN3, QN4, QN5 with respectively hydrolyzed CMC halos diameters of 10, 11, 22, 7, 22 mm) with cellulase activities of 1,31; 1,23; 2,99; 0,99; 2,51 U/ml. The combination of 16S rRNA gene sequences and cultured/biochemical characteristics of the bacteria showed that the five bacteria strains was classified to be Pseudomonas sp. QN1; Pseudomonas sp. QN4; Bacillus sp. QN2; Bacillus sp. QN3; Roseomonas sp. QN5.

scholarly journals Isolation and Characterization of a New Denitrifying Spirillum Capable of Anaerobic Degradation of Phenol

2000 ◽  
Vol 66 (4) ◽  
pp. 1286-1291 ◽  
Author(s):  
Yoshifumi Shinoda ◽  
Yasuyoshi Sakai ◽  
Makiko Ué ◽  
Akira Hiraishi ◽  
Nobuo Kato
Keyword(s):  
Dna Sequences ◽  
Enrichment Culture ◽  
Aerobic Growth ◽  
Ferrous Ions ◽  
Degrading Bacterium ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Enrichment Process ◽  
Better Than

ABSTRACT Two kinds of phenol-degrading denitrifying bacteria,Azoarcus sp. strain CC-11 and spiral bacterial strain CC-26, were isolated from the same enrichment culture after 1 and 3 years of incubation, respectively. Both strains required ferrous ions for growth, but strain CC-26 grew better than strain CC-11 grew under iron-limited conditions, which may have resulted in the observed change in the phenol-degrading bacteria during the enrichment process. Strain CC-26 grew on phenol, benzoate, and other aromatic compounds under denitrifying conditions. Phylogenetic analysis of 16S ribosomal DNA sequences revealed that this strain is most closely related to aMagnetospirillum sp., a member of the α subclass of the class Proteobacteria, and is the first strain of a denitrifying aromatic compound-degrading bacterium belonging to this group. Unlike previously described Magnetospirillumstrains, however, this strain did not exhibit magnetotaxis. It grew on phenol only under denitrifying conditions. Other substrates, such as acetate, supported aerobic growth, and the strain exhibited microaerophilic features.

scholarly journals Isolation and Characterization of Three and Four Ring Pahs Degrading Bacteria from Contaminated Sites, Ankleshwar, Gujarat, India

2015 ◽  
Vol 4 (1) ◽  
pp. 130-140 ◽  
Author(s):  
Jignasha G. Patel ◽  
J.I. Nirmal Kumar ◽  
Rita N Kumar ◽  
Shamiyan R. Khan
Keyword(s):  
Sole Source ◽  
Ex Situ ◽  
Rrna Gene ◽  
Degradation Study ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic ◽  
Bacterial Groups

Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from prolong contaminated Amalakhadi sediment and crude oil polluted soil Telva, near Ankleshwar Gujarat India. Organisms were treated with two-model PAHs compound Anthracene (ANT), and Pyrene (PYR) as the sole source of carbon and energy. Identification of the isolates was carried out based on their morphological and partial 16S rRNA gene sequences, which revealed that the isolates belong to two main bacterial groups: gram-negative pseudomonas indoxyladons and gram-positive, spore-forming group, Bacillus benzoevorans. GC-MS based degradation study demonstrated that P. indoxyladons efficiently degrade 98% of ANT and PYR by 93.2 % when treated with 250 mg L-1. However, B. benzoevorans could tolerate to 200 mg L-1of PYR. Thus, the findings of the study provide novel bacterial sp. having different capacity to degrade model PAHs compounds and further could be utilized for the standardization of bioremediation protocols for ex situ and in situ studies in aquatic as well as terrestrial ecosystem.DOI: http://dx.doi.org/10.3126/ije.v4i1.12184International Journal of Environment Volume-4, Issue-1, Dec-Feb 2014/15, Page: 130-140  

Isolation and characterization of 3-nitrophenol-degrading bacteria associated with rhizosphere of Spirodela polyrrhiza

2012 ◽  
Vol 19 (5) ◽  
pp. 1852-1858 ◽  
Author(s):  
Risky Ayu Kristanti ◽  
Masahiro Kanbe ◽  
Tony Hadibarata ◽  
Tadashi Toyama ◽  
Yasuhiro Tanaka ◽  
...  
Keyword(s):  
Spirodela Polyrrhiza ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3

2019 ◽  
Vol 51 (2) ◽  
pp. 170-178
Author(s):  
Tao Peng ◽  
Qing-Kun Chen ◽  
Jing-Sheng Lun ◽  
Amit Pratush ◽  
Guang-Ming Xiong ◽  
...  
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant

2018 ◽  
Vol 78 (7) ◽  
pp. 1517-1524 ◽  
Author(s):  
Riqiang Li ◽  
Jianxing Wang ◽  
Hongjiao Li
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Removal Rate ◽  
Treatment Plant ◽  
Coking Wastewater ◽  
Naphthalene Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Abstract As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.

scholarly journals Isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium

2005 ◽  
Vol 251 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Li Yang ◽  
Yu-hua Zhao ◽  
Bing-xin Zhang ◽  
Ching-Hong Yang ◽  
Xin Zhang
Keyword(s):  
Degrading Bacterium ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document