Lovastatin Biosynthesis by Aspergillus terreus in a Chemically Defined Medium

ABSTRACT Lovastatin is a secondary metabolite produced by Aspergillus terreus. A chemically defined medium was developed in order to investigate the influence of carbon and nitrogen sources on lovastatin biosynthesis. Among several organic and inorganic defined nitrogen sources metabolized by A. terreus, glutamate and histidine gave the highest lovastatin biosynthesis level. For cultures on glucose and glutamate, lovastatin synthesis initiated when glucose consumption levelled off. When A. terreus was grown on lactose, lovastatin production initiated in the presence of residual lactose. Experimental results showed that carbon source starvation is required in addition to relief of glucose repression, while glutamate did not repress biosynthesis. A threefold-higher specific productivity was found with the defined medium on glucose and glutamate, compared to growth on complex medium with glucose, peptonized milk, and yeast extract.

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Influence of cultivation conditions on IAA - producing activity of endophytic bacterial strains isolated from morning-glory (IPOMOEA PES-CAPRAE (L.) R.Br.)

BIO Web of Conferences ◽

10.1051/bioconf/20213605003 ◽

2021 ◽

Vol 36 ◽

pp. 05003

Author(s):

Nguyen Van Zhang ◽

Nguyen Thi Thu ◽

Vu Thi Linh ◽

V.V. Pylnev ◽

M.I. Popchenko

Keyword(s):

Carbon Source ◽

Culture Medium ◽

Nitrogen Sources ◽

Morning Glory ◽

Bacterial Strains ◽

Cultivation Conditions ◽

Bacillus Mycoides ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Study Results

This work presents the experimental study results of the influence of the culture medium on the ability to IAA synthesis of three endophytic strains TH10R, TH11T, and TH13T from roots of Ipomoea pes-caprae. Three investigated strains give the highest IAA concentration after 96 h of cultivation. A significant increase in IAA biosynthesis was obtained by cultivating the TH10R strain in a medium containing lactose or starch as a carbon source and NH4Cl or KNO3 as a nitrogen source. The TH11T strain produces the maximum amount of IAA, using glucose or xylose and KNO3 or NH4NO3 as carbon and nitrogen sources, respectively. Sucrose is a suitable carbon source for the TH13T strain; on a sucrose-containing medium, the TH13T strain produces the highest IAA amount. The most active strain is TH10R, identified as Bacillus mycoides and named Bacillus mycoides TH10R.

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Production of Phytase by three thermophilic fungi

Indian Journal of Applied Microbiology ◽

10.46798/ijam.2021.v23i01.002 ◽

2021 ◽

Vol 23 (1) ◽

Author(s):

Ranjith Kumar R ◽

Rajendar Reddy ◽

Girisham S ◽

Reddy S.M.

Keyword(s):

Carbon Source ◽

Nitrogen Sources ◽

The Other ◽

Thermomyces Lanuginosus ◽

Thermophilic Fungi ◽

Carbon And Nitrogen Sources ◽

Phytase Production ◽

Carbon And Nitrogen ◽

Cultural Conditions ◽

Other Hand

Production of phytase by three thermophilic fungi, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus under different cultural conditions was assessed. Temperature of 45°C, pH-6.0 were optimum for phytase production by the all three fungi under investigation . Carbon and nitrogen sources for production of phytases by the three thermophilic fungi varied with the fungus. When T. lanuginosus opted for D-glucose followed by D-fructose, T. luteus preferred D-glucose, D-mannose and mannitol for production a phytase. On the other hand, R. pusillus produced maximum phytase during its growth on mannitol and maltose as carbon source. L- asparagine, L- arginine and L-asparatic acid were preferred nitrogen sources for production of phytase by T. lanuginosus. On the other hand T. luteus, opted for L- asparagine, L-glutamic acid and L- glycine for the activity of phytase. R. pusillus produced maximum phytase in medium containing L-argine, L-asparagine and L- asparatic acid.

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Influence of the Different Carbon and Nitrogen Sources on the Production of Biodiesel by Oleaginous Fungi Aspergillus terreus, Aspergillus fumigatus

Baghdad Science Journal ◽

10.21123/bsj.2021.18.2.0225 ◽

2021 ◽

Vol 18 (2) ◽

Keyword(s):

Aspergillus Fumigatus ◽

Aspergillus Terreus ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Oleaginous Fungi

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Nutritional requirements for growth of Cryptoporus volvatus

Canadian Journal of Botany ◽

10.1139/b89-326 ◽

1989 ◽

Vol 67 (8) ◽

pp. 2532-2534

Author(s):

Thomas M. Pettey

Keyword(s):

Carbon Source ◽

Glutamic Acid ◽

Nitrogen Source ◽

Agar Medium ◽

Nitrogen Sources ◽

Nutritional Requirements ◽

Good Growth ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Partial Deficiency

Carbon and nitrogen sources were examined in a defined agar medium to determine the nutritional requirements of Cryptoporus volvatus, a Hymenomycete. Good growth was obtained with D-glucose, D-fructose, D-mannose, D-xylose, or dextrin as the carbon source. Good growth was obtained with ammonium sulfate, casein, peptone, glutamic acid, glycine, lysine, serine, or tyrosine as the nitrogen source. In a defined agar medium, C. volvatus exhibited a deficiency for thiamine, and a partial deficiency for biotin, inositol, and pyridoxine.

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A COMPARISON OF ISOLATES OF CLAVICEPS SPP. FOR THE ABILITY TO GROW AND TO PRODUCE ERGOT ALKALOIDS ON CERTAIN NUTRIENTS

Canadian Journal of Microbiology ◽

10.1139/m60-040 ◽

1960 ◽

Vol 6 (3) ◽

pp. 355-365 ◽

Cited By ~ 5

Author(s):

W. A. Taber ◽

L. C. Vining

Keyword(s):

Carbon Source ◽

Carbon Sources ◽

Ergot Alkaloids ◽

Nitrogen Sources ◽

Claviceps Purpurea ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Different Strains ◽

Do So

Isolates of Claviceps purpurea and Claviceps spp. obtained from various geographical areas were compared for their ability to grow and to produce ergot alkaloids in vitro on various carbon and nitrogen sources. While some differences in utilization of carbon sources for growth were found, there was no observed correlation between utilization of carbohydrates and the capacity to produce ergot alkaloids. The amount of alkaloid produced by different strains depended upon both the carbon and nitrogen sources. In general, those cultures capable of alkaloid production were able to do so on more than one carbon source, but the carbon source allowing greatest production differed from one strain to another. Both producing and non-producing strains could utilize succinic acid as a carbon source for growth, but neither could utilize L-tryptophane as a carbon or nitrogen source for growth.

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Leafy Gall Formation Is Controlled byfasR, an AraC-Type Regulatory Gene inRhodococcus fascians

Journal of Bacteriology ◽

10.1128/jb.182.20.5832-5840.2000 ◽

2000 ◽

Vol 182 (20) ◽

pp. 5832-5840 ◽

Cited By ~ 44

Author(s):

Wim Temmerman ◽

Danny Vereecke ◽

Rozemarijn Dreesen ◽

Marc Van Montagu ◽

Marcelle Holsters ◽

...

Keyword(s):

Gene Expression ◽

Regulatory Gene ◽

Infected Plant ◽

Nitrogen Sources ◽

Defined Medium ◽

Regulatory Proteins ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Rhodococcus Fascians

ABSTRACT Rhodococcus fascians can interact with many plant species and induce the formation of either leafy galls or fasciations. To provoke symptoms, R. fascians strain D188 requires pathogenicity genes that are located on a linear plasmid, pFiD188. Thefas genes are essential for virulence and constitute an operon that encodes, among other functions, a cytokinin synthase gene. Expression of the fas genes is induced by extracts of infected plant tissue only. We have isolated an AraC-type regulatory gene, fasR, located on pFiD188, which is indispensable for pathogenesis and for fas gene expression. The combined results of our experiments show that in vitro expression of thefas genes in a defined medium is strictly regulated and that several environmental factors (pH, carbon and nitrogen sources, phosphate and oxygen content, and cell density) and regulatory proteins are involved. We further show that expression of the fasgenes is controlled at both the transcriptional and the translational levels. The complex expression pattern probably reflects the necessity of integrating a multitude of signals and underlines the importance of the fas operon in the pathogenicity of R. fascians.

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Ability of the fungus Aureobasidium pullulans to produce polysaccharide (pullulan) with presence of different Carbon and Nitrogen sources

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2014.8.1.311 ◽

2014 ◽

Vol 8 (1) ◽

pp. 67-70

Author(s):

Zahraa Ibraheem Muhammed Al_dabbagh ◽

Muhammed Basheer Ismaeil Kassim

Keyword(s):

Amino Acid ◽

Carbon Source ◽

Nitrogen Source ◽

Incubation Period ◽

Aureobasidium Pullulans ◽

Nitrogen Sources ◽

Extracellular Polysaccharide ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Amino Acid Glycine

The effect of incubation period, different carbon and nitrogen sources on the production of the extracellular polysaccharide pullulan by Aureobasidium pullulansNRRL58560 was examined. The results showed that the maximum production of pullulan was obtained 13.76 after 96 hours of incubation. Sucrose as a carbon source gave the highest production of pullulan 31.92 g/l while the highest biomass was obtained when starch was used as a carbon source 16.30 g/l. The amino acid glycine as a nitrogen source gave the highest production of pullulan 34.61 g/l and the highest production of biomass 14.49 g/l was obtained when ammonium chloride was used.

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