scholarly journals Production of Phytase by three thermophilic fungi

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Ranjith Kumar R ◽  
Rajendar Reddy ◽  
Girisham S ◽  
Reddy S.M.
Keyword(s):  
Carbon Source ◽  
Nitrogen Sources ◽  
The Other ◽  
Thermomyces Lanuginosus ◽  
Thermophilic Fungi ◽  
Carbon And Nitrogen Sources ◽  
Phytase Production ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Other Hand

Production of phytase by three thermophilic fungi, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus under different cultural conditions was assessed. Temperature of 45°C, pH-6.0 were optimum for phytase production by the all three fungi under investigation . Carbon and nitrogen sources for production of phytases by the three thermophilic fungi varied with the fungus. When T. lanuginosus opted for D-glucose followed by D-fructose, T. luteus preferred D-glucose, D-mannose and mannitol for production a phytase. On the other hand, R. pusillus produced maximum phytase during its growth on mannitol and maltose as carbon source. L- asparagine, L- arginine and L-asparatic acid were preferred nitrogen sources for production of phytase by T. lanuginosus. On the other hand T. luteus, opted for L- asparagine, L-glutamic acid and L- glycine for the activity of phytase. R. pusillus produced maximum phytase in medium containing L-argine, L-asparagine and L- asparatic acid.

The Screening of Optimal Ganoderma lucidum and the Effect of Medium Substrate on Bioactive Metabolite in Submerged Culture

2012 ◽  
Vol 554-556 ◽  
pp. 887-890
Author(s):  
Mei Lin Cui ◽  
Guo Qing He
Keyword(s):  
Yeast Extract ◽  
Ganoderma Lucidum ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
The Other ◽  
Carbon And Nitrogen Sources ◽  
Bioactive Metabolite ◽  
Carbon And Nitrogen ◽  
Other Hand

To determine an optimal G. lucidum strain, we made the production of mycelia biomass, EPS, IPS, IT, ET as indicators, among the five tested strains, the American G. lucidum was screened, and the production of mycelia biomass, EPS, IPS, IT, ET could reach 2.013g/100 ml, 11.2988 mg/100ml, 23.7800 mg/10ml, 45.5412 mg/100ml, 11.1417 mg/100ml, respectively. On the other hand, according to the screening of carbon and nitrogen sources as well as their concentration, the suitable carbon and nitrogen was 3% malt powder, 1.5% yeast extract. So we can use the fermentation culture for the following research, which was as follows：3% malt powder, 1.5%yeast extract, 0.3% KH2PO4, 0.15% MgSO4, 0.005% VB1.

scholarly journals Influence of cultivation conditions on IAA - producing activity of endophytic bacterial strains isolated from morning-glory (IPOMOEA PES-CAPRAE (L.) R.Br.)

2021 ◽  
Vol 36 ◽  
pp. 05003
Author(s):  
Nguyen Van Zhang ◽  
Nguyen Thi Thu ◽  
Vu Thi Linh ◽  
V.V. Pylnev ◽  
M.I. Popchenko
Keyword(s):  
Carbon Source ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Morning Glory ◽  
Bacterial Strains ◽  
Cultivation Conditions ◽  
Bacillus Mycoides ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Study Results

This work presents the experimental study results of the influence of the culture medium on the ability to IAA synthesis of three endophytic strains TH10R, TH11T, and TH13T from roots of Ipomoea pes-caprae. Three investigated strains give the highest IAA concentration after 96 h of cultivation. A significant increase in IAA biosynthesis was obtained by cultivating the TH10R strain in a medium containing lactose or starch as a carbon source and NH4Cl or KNO3 as a nitrogen source. The TH11T strain produces the maximum amount of IAA, using glucose or xylose and KNO3 or NH4NO3 as carbon and nitrogen sources, respectively. Sucrose is a suitable carbon source for the TH13T strain; on a sucrose-containing medium, the TH13T strain produces the highest IAA amount. The most active strain is TH10R, identified as Bacillus mycoides and named Bacillus mycoides TH10R.

scholarly journals Lovastatin Biosynthesis by Aspergillus terreus in a Chemically Defined Medium

2001 ◽  
Vol 67 (6) ◽  
pp. 2596-2602 ◽  
Author(s):  
Hassan Hajjaj ◽  
Peter Niederberger ◽  
Philippe Duboc
Keyword(s):  
Carbon Source ◽  
Aspergillus Terreus ◽  
Glucose Repression ◽  
Nitrogen Sources ◽  
Glucose Consumption ◽  
Defined Medium ◽  
Specific Productivity ◽  
Chemically Defined Medium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

ABSTRACT Lovastatin is a secondary metabolite produced by Aspergillus terreus. A chemically defined medium was developed in order to investigate the influence of carbon and nitrogen sources on lovastatin biosynthesis. Among several organic and inorganic defined nitrogen sources metabolized by A. terreus, glutamate and histidine gave the highest lovastatin biosynthesis level. For cultures on glucose and glutamate, lovastatin synthesis initiated when glucose consumption levelled off. When A. terreus was grown on lactose, lovastatin production initiated in the presence of residual lactose. Experimental results showed that carbon source starvation is required in addition to relief of glucose repression, while glutamate did not repress biosynthesis. A threefold-higher specific productivity was found with the defined medium on glucose and glutamate, compared to growth on complex medium with glucose, peptonized milk, and yeast extract.

scholarly journals Biosurfactant production by fungi as a sustainable alternative

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Artemisia Carla Santos da Silva ◽  
Patrícia Nunes dos Santos ◽  
Thayse Alves Lima e Silva ◽  
Rosileide Fontenele Silva Andrade ◽  
Galba Maria Campos-Takaki
Keyword(s):  
Metabolic Pathways ◽  
Nitrogen Sources ◽  
Health Food ◽  
Carbon And Nitrogen Sources ◽  
Biotechnological Potential ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Environmentally Safe ◽  
Small Change ◽  
Food Processes

ABSTRACT: A wide variety of bacteria is far more exploited than fungi as biosurfactants (BS) or bioemulsifiers (BE), using renewable sources. BS are considered to be environmentally safe and offer advantages over synthetic surfactants. However, the BS yield depends largely on the metabolic pathways of the microorganisms and the nutritional medium. The production of BS or BE uses several cultural conditions, in which a small change in carbon and nitrogen sources affects the quantity of BS or BE produced. The type and quantity of microbial BS or BE produced depend mainly on the producer organism, and factors such as carbon and nitrogen sources, trace elements, temperature and aeration. The diversity of BS or BE makes it interesting to apply them in the pharmaceutical and cosmetics industries, agriculture, public health, food processes, detergents, when treating oily residues, environmental pollution control and bioremediation. Thus, this paper reviews and addresses the biotechnological potential of yeasts and filamentous fungi for producing, characterizing and applying BS or BE.

Biodegradation of cyanide compounds by a Pseudomonas species (S1)

1999 ◽  
Vol 45 (3) ◽  
pp. 201-208 ◽  
Author(s):  
J K Dhillon ◽  
N Shivaraman
Keyword(s):  
Formic Acid ◽  
Binary Mixtures ◽  
Nitrogen Sources ◽  
Transformation Products ◽  
The Other ◽  
Pseudomonas Sp ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enrichment Technique ◽  
Pseudomonas Species

A Pseudomonas sp. (S1), isolated from soil by an enrichment technique was tested for its potential to degrade different cyanide compounds. Further, biodegradation/biotransformation of binary mixtures of the cyanide compounds by the culture was also studied. The results indicated that the culture could grow on the following nitriles by using them as carbon and nitrogen sources: acetonitrile, butyronitrile, acrylonitrile, adiponitrile, benzonitrile, glutaronitrile, phenylacetonitrile, and succinonitrile. Studies on the biodegradation of these cyanide compounds in binary mixtures showed that the presence of acrylonitrile or KCN delayed the degradation of acetonitrile in a mixture, while none of the other cyanide compounds affected the degradation of one another. The transformation products of the nitriles were their corresponding acids, and similarly, KCN was also directly transformed to formic acid. Studies on the transformation of these cyanide compounds showed that the rate of transformation of nitriles to their corresponding carboxylic acids was acrylonitrile > acetonitrile > adiponitrile > benzonitrile > KCN. This culture has the unique characteristic of transforming representatives of saturated aliphatic, aliphatic olefinic, aromatic, and aralkyl nitriles, as well as alkali cyanide, to their corresponding carboxylic acids.Key words: Pseudomonas sp.(S1), biodegradation, biotransformation, nitriles, cyanide.

Nutritional requirements for growth of Cryptoporus volvatus

1989 ◽  
Vol 67 (8) ◽  
pp. 2532-2534
Author(s):  
Thomas M. Pettey
Keyword(s):  
Carbon Source ◽  
Glutamic Acid ◽  
Nitrogen Source ◽  
Agar Medium ◽  
Nitrogen Sources ◽  
Nutritional Requirements ◽  
Good Growth ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Partial Deficiency

Carbon and nitrogen sources were examined in a defined agar medium to determine the nutritional requirements of Cryptoporus volvatus, a Hymenomycete. Good growth was obtained with D-glucose, D-fructose, D-mannose, D-xylose, or dextrin as the carbon source. Good growth was obtained with ammonium sulfate, casein, peptone, glutamic acid, glycine, lysine, serine, or tyrosine as the nitrogen source. In a defined agar medium, C. volvatus exhibited a deficiency for thiamine, and a partial deficiency for biotin, inositol, and pyridoxine.

scholarly journals Isolation and Selection of Purple Non-Sulfur Bacteria for Nutrient Rich Biomass Production from Wastes

2021 ◽  
Vol 37 (2) ◽  
Author(s):  
Do Binh Minh ◽  
Pham The Hai
Keyword(s):  
Biomass Production ◽  
Morphological Characteristics ◽  
Nitrogen Sources ◽  
Pond Water ◽  
The Other ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Sulfur Bacteria ◽  
Potential Applications ◽  
Excessive Nutrients

Purple nonsulfur bacteria (PNSB) are anoxygenic photosynthetic bacteria, which are able to photoheterotrophically grow in the presence of excessive nutrients. Hence, PNSB can convert organic components, in waste waters into nutrient rich biomass. This suggests the feasibility of converting wastes into valuable products. In this research, method for isolation of PNSB was optimized, and subsequently used for the isolation of  PNSB from domestic waste and pond water samples taken in Hanoi. In addition, the effects of carbon and nitrogen sources and types of waste on biomass and nutrients (protein and carotenes) were also investigated. Four PNSB strains were isolated and based on comparative 16S rDNA analysis and their morphological characteristics, they were identified as Rhodobacter (Rb.) capsulatus, Rhodobacter (Rb.) sphaeroides, Rhodobacter (Rb.) sediminis and Rhodopseudomonas (Rp.) palustris. In terms of the effects of carbon and nitrogen sources on their biomass and nutrient production, glucose or maltose and ammonium chloride or urea were found to be more enhancive than starch and peptone, respectively. Among the studied strains, Rb. capsulatus MD1 and Rb. sphaeroides MD3 showed significantly higher biomass production (up to 0.7 g/l) when growing with various carbon and nitrogen sources, in comparison with the other strains. Moreover,  MD1 and MD3 also produced at least four-fold more carotenoid and up to two-fold more protein in tofu processing wastewater compared with the other wastewater. The results suggest potential applications of the PNSB strains for efficient conversions of organic compounds in wastes into biomass of high nutritional values.  

A COMPARISON OF ISOLATES OF CLAVICEPS SPP. FOR THE ABILITY TO GROW AND TO PRODUCE ERGOT ALKALOIDS ON CERTAIN NUTRIENTS

1960 ◽  
Vol 6 (3) ◽  
pp. 355-365 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Carbon Source ◽  
Carbon Sources ◽  
Ergot Alkaloids ◽  
Nitrogen Sources ◽  
Claviceps Purpurea ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Different Strains ◽  
Do So

Isolates of Claviceps purpurea and Claviceps spp. obtained from various geographical areas were compared for their ability to grow and to produce ergot alkaloids in vitro on various carbon and nitrogen sources. While some differences in utilization of carbon sources for growth were found, there was no observed correlation between utilization of carbohydrates and the capacity to produce ergot alkaloids. The amount of alkaloid produced by different strains depended upon both the carbon and nitrogen sources. In general, those cultures capable of alkaloid production were able to do so on more than one carbon source, but the carbon source allowing greatest production differed from one strain to another. Both producing and non-producing strains could utilize succinic acid as a carbon source for growth, but neither could utilize L-tryptophane as a carbon or nitrogen source for growth.

scholarly journals Ability of the fungus Aureobasidium pullulans to produce polysaccharide (pullulan) with presence of different Carbon and Nitrogen sources

2014 ◽  
Vol 8 (1) ◽  
pp. 67-70
Author(s):  
Zahraa Ibraheem Muhammed Al_dabbagh ◽  
Muhammed Basheer Ismaeil Kassim
Keyword(s):  
Amino Acid ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Aureobasidium Pullulans ◽  
Nitrogen Sources ◽  
Extracellular Polysaccharide ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Amino Acid Glycine

The effect of incubation period, different carbon and nitrogen sources on the production of the extracellular polysaccharide pullulan by Aureobasidium pullulansNRRL58560 was examined. The results showed that the maximum production of pullulan was obtained 13.76 after 96 hours of incubation. Sucrose as a carbon source gave the highest production of pullulan 31.92 g/l while the highest biomass was obtained when starch was used as a carbon source 16.30 g/l. The amino acid glycine as a nitrogen source gave the highest production of pullulan 34.61 g/l and the highest production of biomass 14.49 g/l was obtained when ammonium chloride was used.

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