scholarly journals Lignocellulose Affects Mn2+ Regulation of Peroxidase Transcript Levels in Solid-State Cultures of Pleurotus ostreatus

2002 ◽  
Vol 68 (6) ◽  
pp. 3156-3158 ◽  
Author(s):  
Roni Cohen ◽  
Oded Yarden ◽  
Yitzhak Hadar
Keyword(s):  
Gene Expression ◽  
Solid State ◽  
Direct Effect ◽  
Pleurotus Ostreatus ◽  
Metal Ion ◽  
Transcript Levels ◽  
Peroxidase Gene ◽  
Defined Media ◽  
Cotton Stalks ◽  
Encoding Genes

ABSTRACT The effect of Mn2+ amendment on peroxidase gene expression was studied during Pleurotus ostreatus growth on cotton stalks. Four peroxidase-encoding genes were expressed differentially and in a manner different from that observed in defined media. Mn2+ affects mnp3 expression even 2 h after its addition to the cultures, suggesting a direct effect of the metal ion on expression.

scholarly journals Ligninolytic peroxidase gene expression by Pleurotus ostreatus : Differential regulation in lignocellulose medium and effect of temperature and pH

2014 ◽  
Vol 72 ◽  
pp. 150-161 ◽  
Author(s):  
Elena Fernández-Fueyo ◽  
Raul Castanera ◽  
Francisco J. Ruiz-Dueñas ◽  
María F. López-Lucendo ◽  
Lucía Ramírez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pleurotus Ostreatus ◽  
Differential Regulation ◽  
Effect Of Temperature ◽  
Peroxidase Gene

Abstract 15: Glucose-Mediated Remodeling of Cardiac DNA Methylation

2017 ◽  
Vol 121 (suppl_1) ◽  
Author(s):  
Mark E Pepin ◽  
David K Crossman ◽  
Joseph P Barchue ◽  
Salpy V Pamboukian ◽  
Steven M Pogwizd ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heart Failure ◽  
Dna Methylation ◽  
Diabetic Cardiomyopathy ◽  
Left Ventricular ◽  
Epigenetic Mark ◽  
Gene Transcript ◽  
Transcript Levels ◽  
Glycemic Memory

To identify the role of glucose in the development of diabetic cardiomyopathy, we had directly assessed glucose delivery to the intact heart on alterations of DNA methylation and gene expression using both an inducible heart-specific transgene (glucose transporter 4; mG4H) and streptozotocin-induced diabetes (STZ) mouse models. We aimed to determine whether long-lasting diabetic complications arise from prior transient exposure to hyperglycemia via a process termed “glycemic memory.” We had identified DNA methylation changes associated with significant gene expression regulation. Comparing our results from STZ, mG4H, and the modifications which persist following transgene silencing, we now provide evidence for cardiac DNA methylation as a persistent epigenetic mark contributing to glycemic memory. To begin to determine which changes contribute to human heart failure, we measured both RNA transcript levels and whole-genome DNA methylation in heart failure biopsy samples (n = 12) from male patients collected at left ventricular assist device placement using RNA-sequencing and Methylation450 assay, respectively. We hypothesized that epigenetic changes such as DNA methylation distinguish between heart failure etiologies. Our findings demonstrated that type 2 diabetic heart failure patients (n = 6) had an overall signature of hypomethylation, whereas patients listed as ischemic (n = 5) had a distinct hypermethylation signature for regulated transcripts. The focus of this initial analysis was on promoter-associated CpG islands with inverse changes in gene transcript levels, from which diabetes (14 genes; e.g. IGFBP4) and ischemic (12 genes; e.g. PFKFB3) specific targets emerged with significant regulation of both measures. By combining our mouse and human molecular analyses, we provide evidence that diabetes mellitus governs direct regulation of cellular function by DNA methylation and the corresponding gene expression in diabetic mouse and human hearts. Importantly, many of the changes seen in either mouse type 1 diabetes or human type 2 diabetes were similar supporting a consistent mechanism of regulation. These studies are some of the first steps at defining mechanisms of epigenetic regulation in diabetic cardiomyopathy.

Heavy metal ion complexes with a simple phenolic ligand. Solid state and solution studies

2003 ◽  
Vol 356 ◽  
pp. 203-209 ◽  
Author(s):  
Gianluca Ambrosi ◽  
Mauro Formica ◽  
Vieri Fusi ◽  
Luca Giorgi ◽  
Annalisa Guerri ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Solid State ◽  
Metal Ion ◽  
Heavy Metal Ion ◽  
Metal Ion Complexes ◽  
Solution Studies

The first series of alkali dipyrrinato complexes

2010 ◽  
Vol 88 (8) ◽  
pp. 725-735 ◽  
Author(s):  
Adeeb Al-Sheikh Ali ◽  
Judy Cipot-Wechsler ◽  
Sarah M. Crawford ◽  
Omar Selim ◽  
Rhonda L. Stoddard ◽  
...  
Keyword(s):  
Solid State ◽  
Metal Ion ◽  
Ionic Radius ◽  
Bite Angle ◽  
Out Of Plane ◽  
Ionic Nature ◽  
Meso Position ◽  
Crystallographic Structures ◽  
Solid State Structures ◽  
Sodium And Potassium

The first series of alkali dipyrrinato complexes is reported, encompassing lithium, sodium, and potassium salts of meso-unsubstituted and meso-aryl-substituted derivatives. By varying the substituents at the meso position, the intermolecular distance between the two nitrogen atoms and thus the κ2-N,N-bidentate bite angle was altered, as confirmed by comparison of crystallographic structures of dipyrrin free-bases in the solid-state. The mode of bonding varies as the ionic radius of the metal ion increases: solid-state structures reveal lithium to be accommodated in the plane of the dipyrrinato unit, whilst sodium is accommodated out of plane. The reactivity of analogous lithium, sodium, and potassium dipyrrinato complexes increases as the ionic radius of the metal ion increases, in keeping with the concept that the complexes tend towards an increasingly ionic nature as the size of the alkali metal increases.

Structural and Catalytic Properties of the Alkali Metal Ion-Exchanged Y-Zeolites by 29Si and 27Al Solid-State NMR and FT-IR Spectroscopy

2005 ◽  
Vol 277-279 ◽  
pp. 708-719
Author(s):  
Chang Seop Lee ◽  
Hee Jung Lee ◽  
Sung Woo Choi ◽  
Jahun Kwak ◽  
Charles H.F. Peden
Keyword(s):  
Solid State ◽  
Alkali Metal ◽  
Photoelectron Spectroscopy ◽  
Metal Ion ◽  
Nmr Spectra ◽  
Catalytic Properties ◽  
Nox Reduction ◽  
29Si Nmr ◽  
Y Zeolites ◽  
X Ray

A series of cation exchanged Y-zeolites were prepared by exchanging cations with various alkali (M+, M= Li, Na, K, Cs) metals. The structural and catalytic properties of the alkali metal exchanged Y-zeolites have been investigated by a number of analytical techniques. Comparative elemental analyses were determined by an Energy Dispersive Spectroscopy X-ray (EDS), X-ray Photoelectron Spectroscopy (XPS), Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES) and X-ray Fluorescence (XRF) before and after cation substitution. The framework and non-framework Al coordination and the Si/Al ratios of the Y-zeolites were investigated by MAS Solid-State Nuclear Magnetic Resonance (NMR) spectroscopy. The Al NMR spectra were characterized by two 27Al resonance signals at 12 and 59 ppm, indicating the presence of the non-framework and framework Al respectively. The intensities of these resonances were used to monitor the amount of the framework and non-framework Al species in the series of exchanged zeolites. The 29Si NMR spectra were characterized by four resonance signals at -79, -84, -90, and -95 ppm. Changing the alkali metal cations in the exchanged Y-zeolites significantly altered the extent of the octahedral/tetrahedral coordination and the Si/Al ratio. The Fourier Transform Infrared spectra of the CO2 adsorbed on to the exchanged Y-zeolites showed a low frequency shift, as the atomic number of the exchanged alkali metal increased. In addition, the catalytic activity of these samples for NOx reduction were tested in combination with a non-thermal plasma technique and interpreted based on the above structural and spectroscopic information.

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