scholarly journals Microbial Reduction and Precipitation of Vanadium by Shewanella oneidensis

2003 ◽  
Vol 69 (6) ◽  
pp. 3636-3639 ◽  
Author(s):  
W. Carpentier ◽  
K. Sandra ◽  
I. De Smet ◽  
A. Brig� ◽  
L. De Smet ◽  
...  
Keyword(s):  
Vanadium Pentoxide ◽  
Shewanella Oneidensis ◽  
Microbial Reduction ◽  
Anaerobic Oxidation ◽  
Vanadyl Ion

ABSTRACT Shewanella oneidensis couples anaerobic oxidation of lactate, formate, and pyruvate to the reduction of vanadium pentoxide (VV). The bacterium reduces VV (vanadate ion) to VIV (vanadyl ion) in an anaerobic atmosphere. The resulting vanadyl ion precipitates as a VIV-containing solid.

Impact of Graphitic Structures in Pyrogenic Carbon on Microbial Reduction of Ferrihydrite

2021 ◽  
Author(s):  
wentao yu ◽  
baoliang chen
Keyword(s):  
Electron Transfer ◽  
Pyrolysis Temperature ◽  
Shewanella Oneidensis ◽  
Exchange Capacity ◽  
Microbial Reduction ◽  
Systematic Evaluation ◽  
Extracellular Electron Transfer ◽  
Pyrogenic Carbon ◽  
The Impact

<p>Pyrogenic carbon plays important roles in microbial reduction of ferrihydrite by shuttling electrons in the extracellular electron transfer (EET) processes. Despite its importance, a full assessment on the impact of graphitic structures in pyrogenic carbon on microbial reduction of ferrihydrite has not been conducted. This study is a systematic evaluation of microbial ferrihydrite reduction by Shewanella oneidensis MR-1 in the presence of pyrogenic carbon with various graphitization extents. The results showed that the rates and extents of microbial ferrihydrite reduction were significantly enhanced in the presence of pyrogenic carbon, and increased with increasing pyrolysis temperature. Combined spectroscopic and electrochemical analyses suggested that the rate of microbial ferrihydrite reduction were dependent on the electrical conductivity of pyrogenic carbon (i.e., graphitization extent), rather than the electron exchange capacity. The key role of graphitic structures in pyrogenic carbon in mediating EET was further evidenced by larger microbial electrolysis current with pyrogenic carbon prepared at higher pyrolysis temperatures. This study provides new insights into the electron transfer in the pyrogenic carbon-mediated microbial reduction of ferrihydrite.</p>

scholarly journals Microbial reduction of metal-organic frameworks enables synergistic chromium removal

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Sarah K. Springthorpe ◽  
Christopher M. Dundas ◽  
Benjamin K. Keitz
Keyword(s):  
Metal Oxides ◽  
Metal Organic Frameworks ◽  
Shewanella Oneidensis ◽  
Microbial Reduction ◽  
Pollutant Removal ◽  
Inorganic Materials ◽  
Surface Areas ◽  
Synthetic Materials ◽  
Metal Organic ◽  
Multiple Cycles

AbstractRedox interactions between electroactive bacteria and inorganic materials underpin many emerging technologies, but commonly used materials (e.g., metal oxides) suffer from limited tunability and can be challenging to characterize. In contrast, metal-organic frameworks exhibit well-defined structures, large surface areas, and extensive chemical tunability, but their utility as microbial substrates has not been examined. Here, we report that metal-organic frameworks can support the growth of the metal-respiring bacterium Shewanella oneidensis, specifically through the reduction of Fe(III). In a practical application, we show that cultures containing S. oneidensis and reduced metal-organic frameworks can remediate lethal concentrations of Cr(VI) over multiple cycles, and that pollutant removal exceeds the performance of either component in isolation or bio-reduced iron oxides. Our results demonstrate that frameworks can serve as growth substrates and suggest that they may offer an alternative to metal oxides in applications seeking to combine the advantages of bacterial metabolism and synthetic materials.

Microbial Reduction of Cr (VI)-loaded Schwertmannite by Shewanella oneidensis MR-1

2018 ◽  
Vol 35 (9) ◽  
pp. 727-734 ◽  
Author(s):  
Jingjing Wan ◽  
Chuling Guo ◽  
Zhihong Tu ◽  
Yufei Zeng ◽  
Cong Fan ◽  
...  
Keyword(s):  
Shewanella Oneidensis ◽  
Microbial Reduction

scholarly journals Interpretation of kinetic isotope fractionation between aqueous Fe(II) and ferrihydrite under a high degree of microbial reduction

2020 ◽  
Author(s):  
Lei Jiang ◽  
Chuanjun Wu ◽  
Mingqing Li ◽  
Xuegong Li ◽  
Jiwei Li
Keyword(s):  
Isotope Exchange ◽  
Iron Reduction ◽  
Isotope Fractionation ◽  
Shewanella Oneidensis ◽  
Microbial Reduction ◽  
Fractionation Factor ◽  
Terminal Electron Acceptor ◽  
Iron Mineral ◽  
Dissimilatory Iron Reduction ◽  
Kinetic Fractionation

Abstract. Microbial dissimilatory iron reduction (DIR) often ceases when the degree of iron mineral reduction is low, at which point isotope fractionation occurs between an aqueous Fe(II) solution and a reactive Fe(III) phase on the surface of ferric (oxyhydro) oxides, forming an equilibrium fractionation factor (~ 3 ‰). Recent experimental abiotic studies suggest that Fe(II) adsorption onto the mineral surface may affect the isotope fractionation, which reminds us that the isotope exchange may be greatly inhibited during the DIR process. In this study, ferrihydrite is used as a terminal electron acceptor to conduct Shewanella piezotolerans WP3 and Shewanella oneidensis MR-1 experiments at 0.1 and 15 MPa to ensure a significant variation in the degree of reduction. During the 30-day experiment, the degree of ferrihydrite reduction by S. piezotolerans WP3 is 14 % (at 0.1 MPa) and 8 % (at 15 MPa), whereas the degree of ferrihydrite reduction by S. oneidensis MR-1 is 39 % (at 0.1 MPa) and 36 % (at 15 MPa). Based on the isotope mass balance, the estimated ranges of iron isotope fractionation for S. piezotolerans WP3 and S. oneidensis MR-1 are obtained. The former ranges between −3.58 ‰ and −0.88 ‰ (at 0.1 MPa) and between −2.37 ‰ and −0.66 ‰ (at 15 MPa), and the latter ranges between −0.39 ‰ and 0.10 ‰ (at 0.1 MPa) and between −0.6 ‰ and −0.16 ‰ (at 15 MPa). However, it is difficult to distinguish variations in the same bacteria at 0.1 and 15 MPa due to the large estimation ranges of isotope fractionation. In the S. oneidensis MR-1 experiment, the fractionation factor obtained is significantly different from that obtained in the S. piezotolerans WP3 experiment, indicating that kinetic fractionation occurred. In combination with previous studies, we propose a transient modified Fe(II) adsorption mechanism to explain the isotope fractionation between aqueous Fe(II) and ferrihydrite. When the adsorbed Fe(II) exceeds the surface saturation, the atom (isotope) exchange will be suppressed.

scholarly journals Microbial Reduction of Metal-Organic Frameworks Enables Synergistic Chromium Removal

2018 ◽  
Author(s):  
Sarah K. Springthorpe ◽  
Christopher M. Dundas ◽  
Benjamin K. Keitz
Keyword(s):  
Metal Oxides ◽  
Metal Organic Frameworks ◽  
Reduction Rate ◽  
Shewanella Oneidensis ◽  
Particle Morphology ◽  
Microbial Reduction ◽  
Pollutant Removal ◽  
Extracellular Electron Transfer ◽  
Promising Alternative ◽  
Metal Organic

AbstractMicrobe-material redox interactions underpin many emerging technologies, including bioelectrochemical cells and bioremediation. However, commonly utilized material substrates, such as metal oxides, suffer from a lack of tunability and can be challenging to characterize. In contrast, metal-organic frameworks, a class of porous materials, exhibit well-defined structures, high crystallinity, large surface areas, and extensive chemical tunability. Here, we report that metal-organic frameworks can support the growth of the electroactive bacterium Shewanella oneidensis. Specifically, we demonstrate that Fe(III)-containing frameworks, MIL-100 and Fe-BTC, can be reduced by the bacterium via its extracellular electron transfer pathways and that reduction rate/extent is tied to framework structure, surface area, and particle morphology. In a practical application, we show that cultures containing S. oneidensis and reduced frameworks can remediate lethal concentrations of Cr(VI), and that pollutant removal exceeds the performance of either component in isolation or bioreduced iron oxides. Repeated cycles of Cr(VI) dosing had little effect on bacterial viability or Cr(VI) adsorption capacity, demonstrating that the framework confers protection to the bacteria and that no regenerative step is needed for continued bioremediation. In sum, our results show that metal-organic frameworks can serve as microbial respiratory substrates and suggest that they may offer a promising alternative to metal oxides in applications seeking to combine the advantages of bacterial metabolism and synthetic materials.

Fate of Fe and Cd upon microbial reduction of Cd-loaded polyferric flocs by Shewanella oneidensis MR-1

Chemosphere ◽  
2016 ◽  
Vol 144 ◽  
pp. 2065-2072 ◽  
Author(s):  
Chenchen Li ◽  
Xiaoyun Yi ◽  
Zhi Dang ◽  
Hui Yu ◽  
Tao Zeng ◽  
...  
Keyword(s):  
Shewanella Oneidensis ◽  
Microbial Reduction

scholarly journals Accelerated Microbial Reduction of Azo Dye by Using Biochar from Iron-Rich-Biomass Pyrolysis

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1079 ◽  
Author(s):  
Wenbing Tan ◽  
Lei Wang ◽  
Hanxia Yu ◽  
Hui Zhang ◽  
Xiaohui Zhang ◽  
...  
Keyword(s):  
Azo Dye ◽  
Removal Rate ◽  
Shewanella Oneidensis ◽  
Microbial Reduction ◽  
Biomass Pyrolysis ◽  
Surface Functional Groups ◽  
Redox Active ◽  
Fe Content ◽  
Orange G ◽  
Discharging Capacity

Biochar is widely used in the environmental-protection field. This study presents the first investigation of the mechanism of biochar prepared using iron (Fe)-rich biomass and its impact on the reductive removals of Orange G dye by Shewanella oneidensis MR-1. The results show that biochars significantly accelerated electron transfer from cells to Orange G and thus stimulated reductive removal rate to 72–97%. Both the conductive domains and the charging and discharging of surface functional groups in biochars played crucial roles in the microbial reduction of Orange G to aniline. A high Fe content of the precursor significantly enhanced the conductor performance of the produced biochar and thus enabled the biochar to have a higher reductive removal rate of Orange G (97%) compared to the biochar prepared using low-Fe precursor (75%), but did not promote the charging and discharging capacity of the produced biochar. This study can prompt the search for natural biomass with high Fe content to confer the produced biochar with wide-ranging applications in stimulating the microbial reduction of redox-active pollutants.

scholarly journals Prominent Conductor Mechanism-Induced Electron Transfer of Biochar Produced by Pyrolysis of Nickel-Enriched Biomass

Catalysts ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 573 ◽  
Author(s):  
Wenbing Tan ◽  
Renfei Li ◽  
Hanxia Yu ◽  
Xinyu Zhao ◽  
Qiuling Dang ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Redox Reactions ◽  
Plant Biomass ◽  
Low Cost ◽  
Shewanella Oneidensis ◽  
Microbial Reduction ◽  
Electron Shuttle ◽  
Electron Shuttles ◽  
Ni Content ◽  
Redox Active

Biochar is redox-active and can function as a sustainable electron shuttle in catalyzing relevant redox reactions. It plays a crucial role in environmental remediation. In this work, we used different-nickel (Ni)-level biochars produced by the pyrolysis of plant biomass with correspondingly different Ni levels as extracellular electron shuttles for microbial reduction of ferrihydrite by Shewanella oneidensis MR-1. A high Ni level of the precursor considerably enhanced the conductor mechanism of the produced biochar and thus enabled the biochar to catalyze increased microbial reductions of the Fe(III) mineral, but it did not promote the charging and discharging capacities of the produced biochar. This study can aid in the search for natural biomass with high Ni content to establish low-cost biochars with wide-ranging applications in catalyzing the redox-mediated reactions of pollutants.

scholarly journals Periplasmic Electron Transfer via the c-Type Cytochromes MtrA and FccA of Shewanella oneidensis MR-1

2009 ◽  
Vol 75 (24) ◽  
pp. 7789-7796 ◽  
Author(s):  
Bjoern Schuetz ◽  
Marcus Schicklberger ◽  
Johannes Kuermann ◽  
Alfred M. Spormann ◽  
Johannes Gescher
Keyword(s):  
Electron Transfer ◽  
Cytoplasmic Membrane ◽  
Shewanella Oneidensis ◽  
Microbial Reduction ◽  
Fumarate Reductase ◽  
Mineral Phase ◽  
Membrane Bound ◽  
Growth Experiments

ABSTRACT Dissimilatory microbial reduction of insoluble Fe(III) oxides is a geochemically and ecologically important process which involves the transfer of cellular, respiratory electrons from the cytoplasmic membrane to insoluble, extracellular, mineral-phase electron acceptors. In this paper evidence is provided for the function of the periplasmic fumarate reductase FccA and the decaheme c-type cytochrome MtrA in periplasmic electron transfer reactions in the gammaproteobacterium Shewanella oneidensis. Both proteins are abundant in the periplasm of ferric citrate-reducing S. oneidensis cells. In vitro fumarate reductase FccA and c-type cytochrome MtrA were reduced by the cytoplasmic membrane-bound protein CymA. Electron transfer between CymA and MtrA was 1.4-fold faster than the CymA-catalyzed reduction of FccA. Further experiments showing a bidirectional electron transfer between FccA and MtrA provided evidence for an electron transfer network in the periplasmic space of S. oneidensis. Hence, FccA could function in both the electron transport to fumarate and via MtrA to mineral-phase Fe(III). Growth experiments with a ΔfccA deletion mutant suggest a role of FccA as a transient electron storage protein.

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