scholarly journals Use of Root Organ Cultures To Investigate the Interaction between Glomus intraradices and Pratylenchus coffeae

2003 ◽  
Vol 69 (7) ◽  
pp. 4308-4311 ◽  
Author(s):  
Annemie Elsen ◽  
Stephane Declerck ◽  
Dirk De Waele
Keyword(s):  
Organ Culture ◽  
Glomus Intraradices ◽  
Organ Cultures ◽  
Nematode Reproduction ◽  
Pratylenchus Coffeae ◽  
Root Organ Culture ◽  
Mycorrhizal Development ◽  
Transformed Carrot Roots

ABSTRACT The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was not influenced by the presence of the nematodes.

Production and accumulation of phenylpropanoids in tissue and organ cultures of pimpinella anisum

1988 ◽  
Vol 43 (1-2) ◽  
pp. 42-46 ◽  
Author(s):  
Jürgen Reichling ◽  
Rainer Martin ◽  
Ulla Thron
Keyword(s):  
Organ Culture ◽  
Callus Culture ◽  
Growth Behaviour ◽  
Organ Cultures ◽  
Pimpinella Anisum ◽  
Root Organ Culture ◽  
Entire Plant ◽  
Nutrition Medium ◽  
Special Growth

A leaf-differentiating callus culture and a root organ culture of Pimpinella anisum have been established in liquid nutrition medium. Their behaviour in accumulation of phenylpropanoids was shown to be different from that of the entire plant. This makes them, together with their special growth behaviour, suitable for labelling experiments with the biosynthesis of pseudoisoeugenols

The Culture in vitro of Urogenital Organs of Pleurodeles waltlii

Development ◽  
1962 ◽  
Vol 10 (4) ◽  
pp. 465-470
Author(s):  
Charles L. Foote ◽  
Florence M. Foote
Keyword(s):  
Organ Culture ◽  
Germ Cells ◽  
Culture Medium ◽  
Developmental Stages ◽  
Rana Catesbeiana ◽  
Sex Differentiation ◽  
Organ Cultures ◽  
Tissue And Organ Culture ◽  
Pleurodeles Waltlii

Earlier reports (Foote & Foote, 1958a, b, 1959) describe growth and maintenance in vitro of larval organs, particularly gonads, of Rana catesbeiana and Xenopus laevis. Immature germ cells of both testes and ovaries are well maintained in vitro, especially if the culture medium is supplemented with watersoluble sex-hormonal substances, although germ cells in process of maturation become necrotic. Recently some urogenital organs from the salamander, Pleurodeles waltlii, have been grown in vitro. Tissues and organs from this amphibian might prove to be more suitable for tissue and organ culture investigations than those of Anurans. Animals at three different ages were used in this study: recently hatched larvae, metamorphosing animals, and adults. To determine whether sex differentiation would occur in vitro, trunk portions of young larvae of Pleurodeles waltlii of developmental stages 37–38 (Gallien & Durocher, 1957) were placed in organ cultures.

Synthesis of taurocholate by rat fetal liver in organ culture: effects of cortisol in vitro.

1979 ◽  
Vol 237 (2) ◽  
pp. E177 ◽  
Author(s):  
T O Graham ◽  
D H Van Thiel ◽  
J M Little ◽  
R Lester
Keyword(s):  
Organ Culture ◽  
Dose Response ◽  
Incubation Medium ◽  
Response Pattern ◽  
Fetal Liver ◽  
Dry Weight ◽  
Organ Cultures ◽  
In Vitro Incubation ◽  
Culture Effects

Taurocholate production by fetal hepatic organ cultures was measured by radioimmunoassay. Taurocholate production was maximal on day 1 of in vitro incubation, but was demonstrable in organ cultures maintained for periods up to 15 days. Explants obtained from fetuses of 18 gestational days of age produced only 82 pmol taurocholate per milligram dry weight of tissue during the first 24 h of incubation. Explants obtained from fetuses 21 gestational days of age produced 1,043 pmol taurocholate per milligram dry weight. The presence of cortisol (2.0 X 10(-6) M) in the incubation medium increased synthesis of taurocholate by rat fetal liver in which total taurocholate rose 50-fold above control after 120 h of incubation. In increasing concentrations from 2.0 X 10(-9) M to 2.0 X 10(-7) M, cortisol produced an incremental rise in taurocholate. However, additional increases in cortisol dose failed to provide further stimulation, and taurocholate production was inhibited by cortisol concentrations of 2.0 X 10(-5) M. The results provide further validation for the technique of fetal hepatic organ culture. They demonstrate that taurocholate synthesis is increasing rapidly during the final stages of gestation and show that cortisol augments taurocholate synthesis in a dose-response pattern.

Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

2012 ◽  
Vol 116 (6) ◽  
pp. 729-735 ◽  
Author(s):  
Laura Fernández Bidondo ◽  
Mariana Pergola ◽  
Vanesa Silvani ◽  
Roxana Colombo ◽  
Josefina Bompadre ◽  
...  
Keyword(s):  
Organ Culture ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Monoxenic Culture ◽  
Root Organ Culture

scholarly journals Colonization and Growth Effects of the Mycorrhizal Fungus Glomus intraradicies in a Commercial Nursery Container Production System

2000 ◽  
Vol 18 (4) ◽  
pp. 247-251
Author(s):  
F.T. Davies ◽  
J.A. Saraiva Grossi ◽  
L. Carpio ◽  
A.A. Estrada-Luna
Keyword(s):  
Production System ◽  
Mycorrhizal Fungi ◽  
Host Plants ◽  
Glomus Intraradices ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Dry Mass ◽  
Fertility Level ◽  
Mycorrhizal Development ◽  
Container Production

Abstract The objectives of this research were to demonstrate that mycorrhiza can survive in a commercial nursery container production system, and enhance plant productivity. Four species were used as host plants [Nandina domestica ‘Moon Bay’, Loropetalum chinense variety Rubrum ‘Hinepurpleleaf’ Plumb delight®, Salvia gregii, and Photinia fraseri]. Plants were inoculated with arbuscular mycorrhizal fungi, Glomus intraradices, and grown in a commercial nursery in Texas. For the first 5.5 months, plants were grown in #1 cans containing either 3 kg cu m (5 lbs cu yd) or 4.2 kg cu m (7 lbs cu yd) 24N–4P205–8K20. For the final 6.5 months of the study, plants were in larger containers, all of which contained 4.2 kg cu m (7 lbs cu yd) 24N–4P2O5–8K2O. The commercial inoculum of Glomus intraradices only enhanced growth of N. domestica. The shoot dry mass of mycorrhizal N. domestica plants at 3 kg cu m was the same as non-colonized plants at the higher fertility level of 4.2 cu m. Intraradical hyphae development and colonization (total arbuscules, vesicles/endospores, hyphae) of L. chinense, N. domestica, and S. gregii increased at the higher fertility levels. S. gregii had the greatest mycorrhizal development and a 216% increase in hyphae development and colonization at the higher fertility level.

scholarly journals Functional Significance of Selective Expression of ERα and ERβ in Mammary Gland Organ Culture

2021 ◽  
Vol 22 (23) ◽  
pp. 13151
Author(s):  
Rajendra G. Mehta
Keyword(s):  
Organ Culture ◽  
Functional Significance ◽  
Precancerous Lesions ◽  
Mammary Glands ◽  
Short Exposure ◽  
Preneoplastic Lesions ◽  
Organ Cultures ◽  
Regulated Gene Expression ◽  
Microarray Analyses ◽  
Map Kinase Pathways

Thoracic pair of mammary glands from steroid hormone-pretreated mice respond to hormones structurally and functionally in organ culture. A short exposure of glands for 24 h to 7,12 Dimethylbenz(a)anthracene (DMBA) during a 24-day culture period induced alveolar or ductal lesions. Methods: To differentiate the functional significance of ERα and ERβ, we employed estrogen receptor (ER) knockout mice. We compared the effects of DMBA on the development of preneoplastic lesions in the glands in the absence of ERα (αERKO) and ERβ (βERKO) using an MMOC protocol. Glands were also subjected to microarray analyses. We showed that estradiol can be replaced by EGF for pretreatment of mice. The carcinogen-induced lesions developed under both steroids and EGF pretreatment protocols. The glands from αERKO did not develop any lesions, whereas in βERKO mice in which ERα is intact, mammary alveolar lesions developed. Comparison of microarrays of control, αERKO and βERKO mice showed that ERα was largely responsible for proliferation and the MAP kinase pathways, whereas ERβ regulated steroid metabolism-related genes. The results indicate that ERα is essential for the development of precancerous lesions. Both subtypes, ERα and Erβ, differentially regulated gene expression in mammary glands in organ cultures.

scholarly journals A Protocol on in Vitro Propagation of Arbuscular Mycorrhizal Fungi using Root Organ Culture Technique

2017 ◽  
Vol 31 (1-2) ◽  
pp. 17-24
Author(s):  
Hari Prasad Aryal
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Organ Culture ◽  
Mycorrhizal Fungi ◽  
In Vitro Propagation ◽  
Arbuscular Mycorrhizal ◽  
Culture Conditions ◽  
Culture Technique ◽  
The Past ◽  
Root Organ Culture

 The technique of in vitro propagation of Arbuscular mycorrhizal fungi has been developed over the past few decades and opens up areas of studying plant-fungi interactions. It is a scientific break through, especially for the study of the Arbuscular mycorrhizal fungi, since these obligate symbionts depend on host plant. The objective of this paper is to find out the in vitro culture of Arbuscular Mycorrhizal Fungi using Root Organ Culture technique. Ascertain of root colonization of these fungi could be affected in vitro without undertaking complex and complicated culture conditions. This could form an economically viable technique for root organ culture of Arbuscular mycorrhizal fungi.

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