scholarly journals Cell Surface Display of Human Immunodeficiency Virus Type 1 gp120 on Escherichia coli by Using Ice Nucleation Protein

1999 ◽  
Vol 6 (4) ◽  
pp. 499-503 ◽  
Author(s):  
Young-Don Kwak ◽  
Seung-Ku Yoo ◽  
Eui-Joong Kim
Keyword(s):  
Escherichia Coli ◽  
Human Immunodeficiency Virus ◽  
Surface Display ◽  
Cell Surface Display ◽  
Viral Proteins ◽  
Ice Nucleation ◽  
Ice Nucleation Protein ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT A new system designed for cell surface display of recombinant proteins on Escherichia coli has been evaluated for expression of eukaryotic viral proteins. Human immunodeficiency virus type 1 (HIV-1) gp120 was fused to the C terminus of ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae. Western blotting, immunofluorescence microscopy, fluorescence-activated cell-sorting analysis, whole-cell enzyme-linked immunosorbent assay, and ice nucleation activity assay confirmed the successful expression of HIV-1 gp120 on the surface ofEscherichia coli. This study shows that the INP system can be used for the expression of eukaryotic viral proteins. There is also a possibility that the INP system can be used as an AIDS diagnostic system, an oral vaccine delivery system, and an expression system for various heterologous higher-molecular-weight proteins.

scholarly journals Effect of retinoic acid on HL-60 cells infected with human immunodeficiency virus type 1

Blood ◽  
1994 ◽  
Vol 84 (8) ◽  
pp. 2480-2488 ◽  
Author(s):  
M Semmel ◽  
A Macho ◽  
D Coulaud ◽  
A Alileche ◽  
S Plaisance ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Retinoic Acid ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Viral Proteins ◽  
Mrna Synthesis ◽  
Immunodeficiency Virus ◽  
Infected Cells ◽  
Hiv 1

Abstract HL-60 cells infected with human immunodeficiency virus type 1 (HIV 1) can be induced to differentiate along the granulocyte pathway by retinoic acid. In these cells, HIV mRNA synthesis is stimulated, but synthesis of viral proteins and virus replication are blocked and HIV- infected cells die after becoming apoptotic and/or vacuolized.

scholarly journals Pharmacological Cyclin-Dependent Kinase Inhibitors Inhibit Replication of Wild-Type and Drug-Resistant Strains of Herpes Simplex Virus and Human Immunodeficiency Virus Type 1 by Targeting Cellular, Not Viral, Proteins

2002 ◽  
Vol 76 (15) ◽  
pp. 7874-7882 ◽  
Author(s):  
Luis M. Schang ◽  
Andrew Bantly ◽  
Marie Knockaert ◽  
Farida Shaheen ◽  
Laurent Meijer ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Viral Proteins ◽  
Cyclin Dependent Kinase ◽  
Immunodeficiency Virus ◽  
Simplex Virus ◽  
Hiv 1 ◽  
Hsv 1

ABSTRACT Pharmacological cyclin-dependent kinase (cdk) inhibitors (PCIs) block replication of several viruses, including herpes simplex virus type 1 (HSV-1) and human immunodeficiency virus type 1 (HIV-1). Yet, these antiviral effects could result from inhibition of either cellular cdks or viral enzymes. For example, in addition to cellular cdks, PCIs could inhibit any of the herpesvirus-encoded kinases, DNA replication proteins, or proteins involved in nucleotide metabolism. To address this issue, we asked whether purine-derived PCIs (P-PCIs) inhibit HSV and HIV-1 replication by targeting cellular or viral proteins. P-PCIs inhibited replication of HSV-1 and -2 and HIV-1, which require cellular cdks to replicate, but not vaccinia virus or lymphocytic choriomeningitis virus, which are not known to require cdks to replicate. P-PCIs also inhibited strains of HSV-1 and HIV-1 that are resistant to conventional antiviral drugs, which target viral proteins. In addition, the anti-HSV effects of P-PCIs and a conventional antiherpesvirus drug, acyclovir, were additive, demonstrating that the two drugs act by distinct mechanisms. Lastly, the spectrum of proteins that bound to P-PCIs in extracts of mock- and HSV-infected cells was the same. Based on these observations, we conclude that P-PCIs inhibit virus replication by targeting cellular, not viral, proteins.

Proteases of HIV-1 and MAV Hydrolyze Specifically Human Apo-Hemopexin

2000 ◽  
Vol 65 (7) ◽  
pp. 1191-1197
Author(s):  
Ivan Kluh ◽  
Věra Černá ◽  
Iva Pichová ◽  
Zdeněk Voburka
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Viral Proteins ◽  
Viral Proteases ◽  
Vector Projection ◽  
Immunodeficiency Virus ◽  
Domain Protein ◽  
Hiv 1

The specificities of HIV-1 (Human Immunodeficiency Virus Type 1) and MAV (Myeloblastosis Associated Virus) proteases have been evaluated for their ability to split two-domain protein human apo-hemopexin. Both proteases hydrolyze only one peptidic bond Leu240-Ser241located in the connecting region between two domains. The ability of viral proteases to cleave Leu-Ser bond was confirmed by cleavage of synthetic octapeptide His-Leu-Val-Leu-Ser-Ala-Leu-Thr-NH2covering the susceptible area of human apo-hemopexin. The results demonstrate that the cleavage of Leu-Ser bond is not due to its location in the interdomain region of apo-hemopexin. The cleavable bond Leu-Ser has never been found either in viral or in non-viral proteins. According to the vector projection method this octapeptide was considered as non-hydrolyzable.

scholarly journals Effect of retinoic acid on HL-60 cells infected with human immunodeficiency virus type 1

Blood ◽  
1994 ◽  
Vol 84 (8) ◽  
pp. 2480-2488
Author(s):  
M Semmel ◽  
A Macho ◽  
D Coulaud ◽  
A Alileche ◽  
S Plaisance ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Retinoic Acid ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Viral Proteins ◽  
Mrna Synthesis ◽  
Immunodeficiency Virus ◽  
Infected Cells ◽  
Hiv 1

HL-60 cells infected with human immunodeficiency virus type 1 (HIV 1) can be induced to differentiate along the granulocyte pathway by retinoic acid. In these cells, HIV mRNA synthesis is stimulated, but synthesis of viral proteins and virus replication are blocked and HIV- infected cells die after becoming apoptotic and/or vacuolized.

scholarly journals Transcriptional Errors in Human Immunodeficiency Virus Type 1 Generate Targets for T-Cell Responses

2009 ◽  
Vol 16 (9) ◽  
pp. 1369-1371 ◽  
Author(s):  
Keith E. Garrison ◽  
Stephane Champiat ◽  
Vanessa A. York ◽  
Ashish T. Agrawal ◽  
Esper G. Kallas ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cell ◽  
Human Immunodeficiency Virus Type ◽  
T Cell Responses ◽  
Viral Proteins ◽  
Cell Responses ◽  
Immunodeficiency Virus ◽  
Cryptic Epitopes ◽  
Hiv 1

ABSTRACT We measured T-cell responses to human immunodeficiency virus type 1 (HIV-1) cryptic epitopes encoded by regions of the viral genome not normally translated into viral proteins. T-cell responses to cryptic epitopes and to regions normally spliced out of the HIV-1 viral proteins Rev and Tat were detected in HIV-1-infected subjects.

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