scholarly journals Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Detection and Differentiation of Antibodies against European and North American Porcine Reproductive and Respiratory Syndrome Virus

2002 ◽  
Vol 9 (6) ◽  
pp. 1183-1191 ◽  
Author(s):  
Torsten Seuberlich ◽  
Jon-Duri Tratschin ◽  
Barbara Thür ◽  
Martin A. Hofmann
Keyword(s):  
Immunosorbent Assay ◽  
North American ◽  
Nucleocapsid Protein ◽  
Simultaneous Detection ◽  
The United States ◽  
Respiratory Syndrome Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epidemiological Surveys ◽  
The Us ◽  
The Eu

ABSTRACT Two types of porcine reproductive and respiratory syndrome virus (PRRSV) have been reported, the European type (EU PRRSV) and the North American type (US PRRSV). We developed a dual enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection and differentiation of serum antibodies directed against either of the two PRRSV types. This tandem PRRS ELISA is based on affinity-purified recombinant nucleocapsid protein expressed in Escherichia coli. Sensitivity and specificity were assessed by using the IDEXX HerdChek PRRS ELISA and the indirect immunofluorescence assay as reference tests. A total of 1,571 sera originating from the United States, Europe, and two PRRS-free countries, i.e., Switzerland and New Zealand, were used for validation of the tandem PRRS ELISA. The new test performed at least as well as the reference tests in regard to sensitivity (0.94 for the US PRRS ELISA and 0.93 for the EU PRRS ELISA) and specificity (0.96 for the US PRRS ELISA and 0.99 for the EU PRRS ELISA). Positive sera were correctly differentiated in 582 of 591 cases, indicating a high differentiation capability of this dual ELISA. The robustness and repeatability of the test were assessed and found to be appropriate for diagnostic applications. Taken together, the data indicate that the tandem PRRS ELISA described here is the first differentiation ELISA for PRRSV serology based on recombinant antigen. It is convenient with respect to antigen production, and it is reliable, economical, and highly sensitive and specific. Thus, it is considered to be a powerful tool for routine diagnostics, epidemiological surveys, and outbreak investigations.

scholarly journals The Trend of Scientific Productivity of Chinese, EuropeanUnion, and United States Universities and Private Companies: Does the Future Belong to E-Technology Companies?

Publications ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 18
Author(s):  
Mauro G. Carta ◽  
Matthias C. Angermeyer ◽  
Silvano Tagliagambe
Keyword(s):  
United States ◽  
European Union ◽  
Scientific Productivity ◽  
The United States ◽  
The European Union ◽  
Private Companies ◽  
American Universities ◽  
The Us ◽  
Parametric Statistics ◽  
The Eu

The purpose is to verify trends of scientific production from 2010 to 2020, considering the best universities of the United States, China, the European Union (EU), and private companies. The top 30 universities in 2020 in China, the EU, and the US and private companies were selected from the SCImago institutions ranking (SIR). The positions in 2020, 2015, and 2010 in SIR and three sub-indicators were analyzed by means of non-parametric statistics, taking into consideration the effect of time and group on rankings. American and European Union universities have lost positions to Chinese universities and even more to private companies, which have improved. In 2020, private companies have surpassed all other groups considering Innovation as a sub-indicator. The loss of leadership of European and partly American universities mainly concerns research linked to the production of patents. This can lead to future risks of monopoly that may elude public control and cause a possible loss of importance of research not linked to innovation.

scholarly journals Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS

2005 ◽  
Vol 125 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Masayuki Saijo ◽  
Toshio Ogino ◽  
Fumihiro Taguchi ◽  
Shuetsu Fukushi ◽  
Tetsuya Mizutani ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Nucleocapsid Protein

scholarly journals Development of a Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Quantification of Antibodies against Porcine Reproductive and Respiratory Syndrome Virus

2000 ◽  
Vol 7 (4) ◽  
pp. 700-702 ◽  
Author(s):  
Steven B. Witte ◽  
Cindy Chard-Bergstrom ◽  
Thomas A. Loughin ◽  
Sanjay Kapil
Keyword(s):  
Immunosorbent Assay ◽  
Respiratory Syndrome Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
State University ◽  
Kansas State University ◽  
Porcine Respiratory

ABSTRACT A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

scholarly journals Indirect Enzyme-Linked Immunosorbent Assay based on the nucleocapsid protein of SARS-like coronaviruses

2009 ◽  
Vol 24 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Jun-fa Yuan ◽  
Yan Li ◽  
Hua-jun Zhang ◽  
Peng Zhou ◽  
Zhen-hua Ke ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Enzyme Linked Immunosorbent Assay

Food vs. fuel — A turning point for bioethanol?

2008 ◽  
Vol 56 (4) ◽  
pp. 429-433 ◽  
Author(s):  
K. Harlander
Keyword(s):  
Agricultural Production ◽  
Public Perception ◽  
Turning Point ◽  
The United States ◽  
The Public ◽  
The Us ◽  
Additional Demand ◽  
Oil Crisis ◽  
Emotional Resistance ◽  
The Eu

Bioethanol is made from sugar- or starch-containing plants that are also used in food production. In the public perception this has led to an emotional resistance against biofuels, which in real terms is not substantiated. Generally biofuels are a political product. Triggered by the oil crisis in the 1970s, fuel ethanol programmes were first launched in Brazil and in the United States. Concerns regarding energy security and sustainability, together with the option of new markets for surplus agricultural production, have led to similar measures in the EU and other countries in recent years. Accordingly, the industry invested heavily in new bioethanol plants — especially in the US — and created an additional demand for maize and wheat, with some record-breaking prices noted in late 2007. A look back into statistics shows a drastic decline in real prices for decades, which have now simply returned to the level of 30 years ago. The grain used for bioethanol is currently only 1.6% in the EU and is therefore unlikely to be the real driver of price development. The European Commission concludes in its review of agricultural markets that Europe can do both: nutrition and biofuels.

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Antibody to Epizootic Hemorrhagic Disease of Deer Virus

2000 ◽  
Vol 12 (2) ◽  
pp. 142-145 ◽  
Author(s):  
James O. Mecham ◽  
Michael M. Jochim
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hemorrhagic Disease ◽  
Structural Protein ◽  
Serum Samples ◽  
Epizootic Hemorrhagic Disease ◽  
Diagnostic Potential ◽  
The Us ◽  
Serotype 1 ◽  
Infected Cells

An enzyme-linked immunosorbent assay has been developed to detect antibodies to epizootic hemorrhagic disease of deer virus (EHDV). The assay incorporates a monoclonal antibody to EHDV serotype 2 (EHDV-2) that demonstrates specificity for the viral structural protein, VP7. The assay was evaluated with sequential sera collected from cattle experimentally infected with EHDV serotype 1 (EHDV-1) and EHDV-2, as well as the four serotypes of bluetongue virus (BTV), BTV-10, BTV-11, BTV-13, and BTV-17, that currently circulate in the US. A competitive and a blocking format as well as the use of antigen produced from both EHDV-1-and EHDV-2-infected cells were evaluated. The assay was able to detect specific antibody as early as 7 days after infection and could differentiate animals experimentally infected with EHDV from those experimentally infected with BTV. The diagnostic potential of this assay was demonstrated with field-collected serum samples from cattle, deer, and buffalo.

scholarly journals Energy Security Problem amid Global Energy Transition

2021 ◽  
Vol 21 (4) ◽  
pp. 772-784
Author(s):  
Yury V. Borovsky
Keyword(s):  
Energy Security ◽  
Energy Use ◽  
Rapid Development ◽  
Energy Transition ◽  
The United States ◽  
Low Carbon ◽  
Security Threat ◽  
The Us ◽  
The Impact ◽  
The Eu

In the early 2020s the worlds transition from carbon-intensive to climate-neutral energy use has already become a discernible and a difficult-to-reverse process. With Joe Bidens election as US president, the United States have returned to the Paris Climate Agreement and have become a key driver of this process (along with the EU and China). As a result, the international community has reached a consensus on the ongoing energy transition. This process will require considerable effort and may take several decades. Nevertheless, the impact of energy transition on traditional approaches to energy security, which emerged largely as a result of the global oil crises of the 1970s and 1980s and are centered around the supply of fossil fuels, is already a relevant research topic. This problem is examined relying on the relevant terminological, theoretical and factual material. The article concludes that energy transition will ultimately undermine the carbon paradigm that has underpinned energy security policies since the 1970s. Rapid development of renewable and other low-carbon energy sources will certainly remove key energy security risks of energy importers and, possibly, allow them to achieve energy independence. However, a post-carbon era may also generate new risks. For countries that rely heavily on oil, gas and coal exports, energy transition will result in the loss of markets and revenues. It may present an energy security threat for them as well as it will require a costly and technologically complex process of the energy sector decarbonization. Some exporters, especially those with high fuel rents and insufficient financial reserves, may face serious economic and social upheavals as a result of energy transition. The EU and the US energy transition policies reflect provisions of all three fundamental international relations theoretical paradigms, including realism. This means that the EU and the US policy, aimed at promoting climate agenda, may be expected to be rather tough and aggressive. China as the third key player in energy transition is still following a liberal course; however, it may change in the future.

scholarly journals US and EU politics «eastern partnership»

2016 ◽  
pp. 33-42 ◽  
Author(s):  
Olena Skrypnyk
Keyword(s):  
United States ◽  
Human Rights ◽  
The United States ◽  
Economic Situation ◽  
Eastern Partnership ◽  
The Us ◽  
The Eu

In this article to analyzes the policy of the European Union’s «Eastern Partnership». Determined US relation to the initiative of the EU. Characterized four summits the EU «Eastern Partnership» and followed the US response to these summits. The attention to Ukraine’s participation in the summit of the EU and the US position on this issue. Determined that the United States strongly supports the EU initiative «Eastern Partnership», especially in order to spread in the countries of the «Eastern Partnership» democracy, ensure human rights and freedoms, and to improve the socio-economic situation of these countries.

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