scholarly journals Quantitative Measurement of C6 Antibody following Antibiotic Treatment of Borrelia burgdorferi Antibody-Positive Nonclinical Dogs

2007 ◽  
Vol 15 (1) ◽  
pp. 115-119 ◽  
Author(s):  
Steven A. Levy ◽  
Thomas P. O'Connor ◽  
Jancy L. Hanscom ◽  
Paulette Shields ◽  
Leif Lorentzen ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Therapy ◽  
Antibiotic Treatment ◽  
Untreated Control ◽  
Quantitative Measurement ◽  
Antibody Test ◽  
Treated Group ◽  
Data Points ◽  
Antibody Levels

ABSTRACTThe detection of antibody to theBorrelia burgdorferiC6peptide by use of enzyme-linked immunoassays is a widely accepted method for the diagnosis of Lyme disease spirochete infection in dogs and in humans. Antibody to the C6peptide is highly specific forB. burgdorferiand declines following treatment of dogs and humans exposed toB. burgdorferi. A quantitative assay for determining C6antibody levels was developed and used to measure changes in antibody levels following antibiotic treatment ofB. burgdorferiantibody-positive nonclinical dogs. One hundred thirty-two client-owned dogs were used in the study; 64 were negative, 53 of 68 positive animals received treatment, and 15 were untreated controls. Test sera were collected at 3, 6, and 12 months from seropositive dogs receiving treatment and untreated controls. Dogs in the treated group were assigned to moderate-to-high (≥29 U/ml)- and low (<29 U/ml)-C6-level groups because the change in the C6level after treatment was dependent on the level prior to treatment. There were significant declines in the 30 dogs with moderate-to-high initial C6levels that exceeded the maximal declines of the untreated control dogs in all cases at 6 months (16 data points) and 12 months (29 data points) posttreatment. There was little change in C6level following antibiotic therapy in the 23 dogs with low initial C6levels. The quantitative C6antibody test can be used to measure changes in C6antibody levels following treatment of antibody-positive nonclinical dogs.

scholarly journals Antibiotic Treatment of Animals Infected with Borrelia burgdorferi

2009 ◽  
Vol 22 (3) ◽  
pp. 387-395 ◽  
Author(s):  
Gary P. Wormser ◽  
Ira Schwartz
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Therapy ◽  
Antibiotic Treatment ◽  
Short Term Memory ◽  
Scientific Evidence ◽  
Disease Symptoms ◽  
Chronic Lyme Disease ◽  
Methodological Concern ◽  
Uncertain Etiology

SUMMARY Despite resolution of the objective manifestations of Lyme disease after antibiotic treatment, a minority of patients have fatigue, musculoskeletal pain, and/or difficulties with concentration or short-term memory of uncertain etiology; these are called post-Lyme disease symptoms or, in more severe cases, post-Lyme disease syndrome or “chronic Lyme disease.” Several recent studies in which Borrelia burgdorferi-infected animals were treated with antibiotic therapy have demonstrated the presence of PCR positivity for B. burgdorferi DNA in the absence of culture positivity. In mice that were treated with antibiotic therapy, residual spirochetes could be taken up by ticks during a blood meal and could be transmitted to SCID mice. These spirochetes are attenuated; their presence is not associated with either inflammation or disease. In this review the methodology and findings of these studies are critically analyzed, and the significance of the results with regard to human Lyme disease is evaluated, with special emphasis on whether these studies provide useful insights into post-Lyme disease syndrome. A serious methodological concern is the failure to consider the pharmacokinetic-pharmacodynamic properties of the antibiotic in choosing the dosage regimen used. We conclude that there is no scientific evidence to support the hypothesis that such spirochetes, should they exist in humans, are the cause of post-Lyme disease syndrome.

scholarly journals Efficacy of an Evernimicin (SCH27899) In Vitro and in an Animal Model of Lyme Disease

2001 ◽  
Vol 45 (3) ◽  
pp. 936-937 ◽  
Author(s):  
Charles S. Pavia ◽  
Gary P. Wormser ◽  
John Nowakowski ◽  
Anthony Cacciapuoti
Keyword(s):  
Animal Model ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Treatment ◽  
Murine Model ◽  
C3h Mice

ABSTRACT The MICs of evernimicin at which 90% of Borrelia burgdorferi patient isolates were inhibited ranged from 0.1 to 0.5 μg/ml. Evernimicin was as effective as ceftriaxone againstB. burgdorferi in a murine model of experimental Lyme disease. As assessed by culturing the urinary bladders of infected C3H mice, no live Borrelia isolates were recoverable following antibiotic treatment.

scholarly journals Antibodies to Borrelia burgdorferi OspA, OspC, OspF, and C6 Antigens as Markers for Early and Late Infection in Dogs

2012 ◽  
Vol 19 (4) ◽  
pp. 527-535 ◽  
Author(s):  
Bettina Wagner ◽  
Heather Freer ◽  
Alicia Rollins ◽  
David Garcia-Tapia ◽  
Hollis N. Erb ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
The United States ◽  
Sensu Stricto ◽  
Antibody Responses ◽  
Surface Proteins ◽  
Early Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Content Type ◽  
Antibody Levels

ABSTRACTLyme disease in the United States is caused byBorrelia burgdorferisensu stricto, which is transmitted to mammals by infected ticks.Borreliaspirochetes differentially express immunogenic outer surface proteins (Osp). Our aim was to evaluate antibody responses to Osp antigens to aid the diagnosis of early infection and the management of Lyme disease. We analyzed antibody responses during the first 3 months after the experimental infection of dogs using a novel multiplex assay. Results were compared to those obtained with two commercial assays detecting C6 antigen. Multiplex analysis identified antibodies to OspC and C6 as early as 3 weeks postinfection (p.i.) and those to OspF by 5 weeks p.i. Antibodies to C6 and OspF increased throughout the study, while antibodies to OspC peaked between 7 and 11 weeks p.i. and declined thereafter. A short-term antibody response to OspA was observed in 3/8 experimentally infected dogs on day 21 p.i. Quant C6 enzyme-linked immunosorbent assay (ELISA) results matched multiplex results during the first 7 weeks p.i.; however, antibody levels subsequently declined by up to 29%. Immune responses then were analyzed in sera from 125 client-owned dogs and revealed high agreement between antibodies to OspF and C6 as robust markers for infection. Results from canine patient sera supported that OspC is an early infection marker and antibodies to OspC decline over time. The onset and decline of antibody responses toB. burgdorferiOsp antigens and C6 reflect their differential expression during infection. They provide valuable tools to determine the stage of infection, treatment outcomes, and vaccination status in dogs.

scholarly journals Eucaryotic cells protect Borrelia burgdorferi from the action of penicillin and ceftriaxone but not from the action of doxycycline and erythromycin.

1996 ◽  
Vol 40 (6) ◽  
pp. 1552-1554 ◽  
Author(s):  
P Brouqui ◽  
S Badiaga ◽  
D Raoult
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Treatment ◽  
Intracellular Location

Despite appropriate antibiotic treatment, Lyme disease patients may have relapses or may develop chronic manifestations. The intracellular location of Borrelia burgdorferi suggests that antibiotics that penetrate cells will have greater efficiency. Doxycycline or erythromycin was more effective than penicillin or ceftriaxone in killing B. burgdorferi when the organism was grown in the presence of eucaryotic cells.

scholarly journals Significantly Improved Accuracy of Diagnosis of Early Lyme Disease by Peptide Enzyme-Linked Immunosorbent Assay Based on the Borreliacidal Antibody Epitope of Borrelia burgdorferi OspC

2008 ◽  
Vol 15 (6) ◽  
pp. 981-985 ◽  
Author(s):  
Dean A. Jobe ◽  
Steven D. Lovrich ◽  
Krista E. Asp ◽  
Michelle A. Mathiason ◽  
Stephanie E. Albrecht ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Western Blot ◽  
Immunosorbent Assay ◽  
Antibody Test ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Terminus ◽  
Igm Elisa ◽  
Improved Accuracy

ABSTRACT Highly specific borreliacidal antibodies are induced by infection with Borrelia burgdorferi, and the immunodominant response during early Lyme disease is specific for an epitope within the 7 amino acids nearest the C terminus of OspC. We evaluated the ability of an enzyme-linked immunosorbent assay (ELISA) based on a synthetic peptide (OspC7) that matched the region to detect the response and compared the sensitivity during early Lyme disease to that for an FDA-approved Western blot. When the optical density value was adjusted to 98% specificity based on the results from testing normal or uncharacterized sera (n = 236) or sera from patients with blood factors or illnesses that commonly produce antibodies that cross-react with B. burgdorferi antigens (n = 77), 115 (73%) of 157 sera from patients likely to have early Lyme disease were positive for immunoglobulin M (IgM) antibodies and 17 (11%) also had IgG antibodies. In addition, the IgM ELISA reactivities and the titers of antibodies detected by a flow cytometric borreliacidal antibody test correlated closely (r = 0.646). Moreover, the IgM ELISA was significantly more sensitive (P < 0.001) than the Western blot procedure. The findings therefore confirmed that the peptide IgM ELISA detected OspC borreliacidal antibodies and provided strong evidence that the test can eliminate the necessity for confirming early Lyme disease by a supplementary test such as Western blotting.

Changes in Borrelia burgdorferi ELISA antibody over time in both antibiotic treated and untreated horses

2012 ◽  
Vol 60 (4) ◽  
pp. 421-429 ◽  
Author(s):  
Thomas Divers ◽  
Amy Grice ◽  
Hussni Mohammed ◽  
Amy Glaser ◽  
Bettina Wagner
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Treatment ◽  
Treatment Success ◽  
Clinical Signs ◽  
Prolonged Treatment ◽  
Similar Time ◽  
Time Period ◽  
Marked Decline ◽  
Small Decline

Changes in ELISA serology are frequently used to determine antibiotic treatment success for Lyme disease in horses. This concept was based upon a previous report showing a marked decline in ELISA values in experimentally infected and antibiotic-treated ponies. Changes in Lyme serology following antibiotic treatment in naturally infected horses have not been reported. The objective of this study was to compareBorreliaELISA antibody concentrations in naturally exposed horses both before and following antibiotic treatment for Lyme disease. A retrospective study was performed comparing oxytetracycline- or doxycyclinetreated (n = 68) and untreated (n = 183) horses from a single equine practice and their change inBorreliaELISA values over a similar time period. Antibiotictreated horses had a decline in ELISA values in comparison to control horses (P ≤ 0.05) and untreated horses were twice as likely to have their ELISA values increase (OR = 0.5; 95% C.I. = 0.3–0.9) compared to treated horses. The magnitude of the decline in ELISA units following treatments was small compared to that previously reported in experimentally infected and treated ponies. Field-exposed horses with highBorrelia burgdorferiELISA values who are treated with either oxytetracycline or doxycycline can be expected to have only a small decline in ELISA values following treatment. Persistently high ELISA titres following appropriate treatments for Lyme disease may not, without appropriate clinical signs, be a reason for more prolonged treatment.

scholarly journals Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease

2018 ◽  
Author(s):  
Marianne J. Middelveen ◽  
Eva Sapi ◽  
Jennie Burke ◽  
Katherine R. Filush ◽  
Agustin Franco ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Therapy ◽  
Detection Methods ◽  
Molecular Testing ◽  
Disease Symptoms ◽  
Control Subjects ◽  
Blinded Manner ◽  
Polymerase Chain ◽  
Lyme Spirochete

Introduction: Lyme disease is a tickborne illness that generates controversy among medical providers and researchers. One of the key topics of debate is the existence of persistent infection with the Lyme spirochete, Borrelia burgdorferi, in patients who have been treated with recommended doses of antibiotics yet remain symptomatic. Persistent spirochetal infection despite antibiotic therapy has recently been demonstrated in non-human primates. We present evidence of persistent Borrelia infection despite antibiotic therapy in patients with ongoing Lyme disease symptoms. Materials &amp; Methods: In this pilot study, culture of body fluids and tissues was performed in a randomly selected group of 12 patients with persistent Lyme disease symptoms who had been treated or who were being treated with antibiotics. Cultures were also performed on a group of 10 control subjects without Lyme disease. The cultures were subjected to corroborative microscopic, histopathological and molecular testing for Borrelia organisms in four independent laboratories in a blinded manner. Results: Motile spirochetes identified histopathologically as Borrelia were detected in culture specimens, and these spirochetes were genetically identified as&nbsp;Borrelia burgdorferi&nbsp;by three distinct polymerase chain reaction (PCR) methods. Spirochetes identified as&nbsp;Borrelia burgdorferi&nbsp;were cultured from the blood of seven subjects, from the genital secretions of ten subjects, and from a skin lesion of one subject. Cultures from control subjects without Lyme disease were negative for Borrelia using these methods. Conclusions: Using multiple corroborative detection methods, we showed that patients with persistent Lyme disease symptoms may have ongoing spirochetal infection despite antibiotic treatment, similar to findings in non-human primates. The optimal treatment for persistent Borrelia infection remains to be determined.

Een sportieve vijftiger met brutale syncope ten gevolge van Lyme carditis

2021 ◽  
Author(s):  
C. BAESTAENS ◽  
S. HELLEMANS
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Therapy ◽  
Medical History ◽  
Permanent Pacemaker ◽  
Serological Testing ◽  
Conduction Disturbances ◽  
Lyme Carditis ◽  
Life Threatening ◽  
Life Threatening Complication

A sportive man in his 50's from Philadelphia with a brutal syncope from Lyme carditis In the case of a syncope at a young age in a patient with no medical history - especially if he or she comes from endemic regions - Lyme disease should be excluded through serological testing. Lyme carditis is a potentially life-threatening complication after infection with the bacterium Borrelia burgdorferi. This is shown in the discussed case of a 51-year-old sportive man who registered for a consultation after a brutal syncope. Under antibiotic therapy, the severe conduction disturbances progressively cleared up and a permanent pacemaker could be avoided.

scholarly journals Lyme Disease in Taiwan: First Human Patient with Characteristic Erythema Chronicum Migrans Skin Lesion

1998 ◽  
Vol 36 (3) ◽  
pp. 807-808 ◽  
Author(s):  
Chien-Ming Shih ◽  
Jin-Choung Wang ◽  
Li-Lian Chao ◽  
Trong-Neng Wu
Keyword(s):  
Lyme Disease ◽  
Skin Lesion ◽  
Borrelia Burgdorferi ◽  
Knee Joint ◽  
Antibiotic Treatment ◽  
Human Patient ◽  
Tick Vector ◽  
Serologic Tests ◽  
Northern Taiwan

We report herein the first laboratory-diagnosed case of Lyme disease in a human in Taiwan. A 45-year-old Taiwanese man living in Taipei, in northern Taiwan, had an expanding skin lesion (measuring 23 by 15 cm) on his abdomen for 2 to 3 weeks and recurrent attacks of pain and swelling of the knee joint. Serologic tests indicated a significantly elevated titer of antibody to Borrelia burgdorferi. After appropriate antibiotic treatment for 3 weeks, the skin lesion was cured and the joint swelling was improved. Although several strains of Borrelia spirochetes had been isolated from rodents (Rattus losea) in Taiwan, the tick vector responsible for the transmission remains to be identified.

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