scholarly journals Recombinant Protein-Based Assays for Detection of Antibodies to Severe Acute Respiratory Syndrome Coronavirus Spike and Nucleocapsid Proteins

2007 ◽  
Vol 14 (3) ◽  
pp. 331-333 ◽  
Author(s):  
Lia M. Haynes ◽  
Congrong Miao ◽  
Jennifer L. Harcourt ◽  
Joel M. Montgomery ◽  
Mai Quynh Le ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Recombinant Protein ◽  
Sensitivity And Specificity ◽  
Immunoglobulin G ◽  
High Sensitivity ◽  
Kinetic Studies ◽  
Spike Protein ◽  
Sars Coronavirus ◽  
Nucleocapsid Proteins

ABSTRACT Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

scholarly journals Specific Immunoglobulin G Antibody Detected in Umbilical Blood and Amniotic Fluid from a Pregnant Woman Infected by the Coronavirus Associated with Severe Acute Respiratory Syndrome

2004 ◽  
Vol 11 (6) ◽  
pp. 1182-1184 ◽  
Author(s):  
Xiugao Jiang ◽  
Xing Gao ◽  
Han Zheng ◽  
Meiying Yan ◽  
Weili Liang ◽  
...  
Keyword(s):  
Pregnant Woman ◽  
Severe Acute Respiratory Syndrome ◽  
Amniotic Fluid ◽  
Immunoglobulin G ◽  
Maternal Blood ◽  
Sars Coronavirus ◽  
Umbilical Blood ◽  
Specific Immunoglobulin

ABSTRACT Specific immunoglobulin G antibody for severe acute respiratory syndrome (SARS) coronavirus was detected in maternal blood, umbilical blood, and amniotic fluid from a pregnant SARS patient. Potential protection of fetus from infection was suggested.

A Trimerizing GxxxG Motif Is Uniquely Inserted in the Severe Acute Respiratory Syndrome (SARS) Coronavirus Spike Protein Transmembrane Domain†

Biochemistry ◽  
2006 ◽  
Vol 45 (38) ◽  
pp. 11349-11356 ◽  
Author(s):  
Eyal Arbely ◽  
Zvi Granot ◽  
Itamar Kass ◽  
Joseph Orly ◽  
Isaiah T. Arkin
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Transmembrane Domain ◽  
Spike Protein ◽  
Sars Coronavirus ◽  
Gxxxg Motif

scholarly journals Heterologous viral RNA export elements improve expression of severe acute respiratory syndrome (SARS) coronavirus spike protein and protective efficacy of DNA vaccines against SARS

Virology ◽  
2007 ◽  
Vol 363 (2) ◽  
pp. 288-302 ◽  
Author(s):  
Benoît Callendret ◽  
Valérie Lorin ◽  
Pierre Charneau ◽  
Philippe Marianneau ◽  
Hugues Contamin ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Dna Vaccines ◽  
Protective Efficacy ◽  
Viral Rna ◽  
Spike Protein ◽  
Sars Coronavirus ◽  
Rna Export

scholarly journals Mosaic Evolution of the Severe Acute Respiratory Syndrome Coronavirus

2004 ◽  
Vol 78 (1) ◽  
pp. 76-82 ◽  
Author(s):  
John Stavrinides ◽  
David S. Guttman
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Phylogenetic Analyses ◽  
Protein A ◽  
Infectious Agent ◽  
Sars Coronavirus ◽  
Receptor Binding Site ◽  
Nucleocapsid Proteins ◽  
Spike Gene ◽  
Novel Coronavirus ◽  
Tract Infections

ABSTRACT Severe acute respiratory syndrome (SARS) is a deadly form of pneumonia caused by a novel coronavirus, a viral family responsible for mild respiratory tract infections in a wide variety of animals including humans, pigs, cows, mice, cats, and birds. Analyses to date have been unable to identify the precise origin of the SARS coronavirus. We used Bayesian, neighbor-joining, and split decomposition phylogenetic techniques on the SARS virus replicase, surface spike, matrix, and nucleocapsid proteins to reveal the evolutionary origin of this recently emerging infectious agent. The analyses support a mammalian-like origin for the replicase protein, an avian-like origin for the matrix and nucleocapsid proteins, and a mammalian-avian mosaic origin for the host-determining spike protein. A bootscan recombination analysis of the spike gene revealed high nucleotide identity between the SARS virus and a feline infectious peritonitis virus throughout the gene, except for a 200- base-pair region of high identity to an avian sequence. These data support the phylogenetic analyses and suggest a possible past recombination event between mammalian-like and avian-like parent viruses. This event occurred near a region that has been implicated to be the human receptor binding site and may have been directly responsible for the switch of host of the SARS coronavirus from animals to humans.

scholarly journals Longitudinal Analysis of Severe Acute Respiratory Syndrome (SARS) Coronavirus-Specific Antibody in SARS Patients

2005 ◽  
Vol 12 (12) ◽  
pp. 1455-1457 ◽  
Author(s):  
Shan-Chwen Chang ◽  
Jann-Tay Wang ◽  
Li-Min Huang ◽  
Yee-Chun Chen ◽  
Chi-Tai Fang ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Longitudinal Analysis ◽  
Specific Antibody ◽  
Immunoglobulin G ◽  
Disease Onset ◽  
Blood Sampling ◽  
Sars Coronavirus ◽  
Serum Antibodies ◽  
Igg Antibody ◽  
Igm Antibody

ABSTRACT The serum antibodies to severe acute respiratory syndrome (SARS) coronavirus of 18 SARS patients were checked at 1 month and every 3 months after disease onset. All of them except one, who missed blood sampling at 1 month, tested positive for the immunoglobulin G (IgG) antibody at 1 month. Fifteen out of 17 tested positive for the IgM antibody at 1 month. The serum IgM antibody of most patients became undetectable within 6 months after the onset of SARS. The IgG antibody of all 17 patients, whose serum was checked 1 year after disease onset, remained positive.

scholarly journals Longitudinal Profile of Immunoglobulin G (IgG), IgM, and IgA Antibodies against the Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein in Patients with Pneumonia Due to the SARS Coronavirus

2004 ◽  
Vol 11 (4) ◽  
pp. 665-668 ◽  
Author(s):  
Patrick C. Y. Woo ◽  
Susanna K. P. Lau ◽  
Beatrice H. L. Wong ◽  
Kwok-hung Chan ◽  
Chung-ming Chu ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Indirect Immunofluorescence ◽  
Immunoglobulin G ◽  
Nucleocapsid Protein ◽  
Baseline Level ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sars Coronavirus ◽  
Iga Antibodies

ABSTRACT By using a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein-based enzyme-linked immunosorbent assay (ELISA) and serum specimens serially collected (from day 0 to day 240 after symptom onset) from patients with pneumonia due to SARS-CoV, we analyzed the longitudinal profiles of immunoglobulin G (IgG), IgM, and IgA antibodies against the SARS-CoV nucleocapsid protein in patients with pneumonia due to SARS-CoV. For IgG, the median optical density at 450 nm (OD450) turned positive at day 17 and a biphasic response was observed. At day 240, all patients were still positive for anti-nucleocapsid protein IgG antibody. For IgM, the median OD450 turned positive at day 20.5, peaked at about day 80, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgM antibody. For IgA, the median OD450 turned positive at day 17, peaked at about day 50, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgA antibody. The time of seroconversion detected by the recombinant SARS-CoV nucleocapsid protein-based ELISA and that detected by indirect immunofluorescence assay were similar. The median times of seroconversion for IgG, IgM, and IgA detected by the indirect immunofluorescence assay were 17 days (17 days by ELISA), 16.5 days (20.5 days by ELISA), and 17.5 days (17 days by ELISA), respectively, after disease onset. One, four, and one of the six patients who died did not produce any IgG, IgM, and IgA antibodies against the nucleocapsid protein of SARS-CoV, respectively, although these antibodies were detected in all six patients by the indirect immunofluorescence assay. Further studies should be performed to see whether SARS-CoV nucleocapsid protein antibody positivity has any prognostic significance.

scholarly journals Absence of infection in asymptomatic contacts of index SARS case in France

2006 ◽  
Vol 11 (1) ◽  
pp. 9-10 ◽  
Author(s):  
S Le Vu ◽  
Y Yazdanpanah ◽  
D Bitar ◽  
J Emmanuelli ◽  
I Bonmarin ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Blood Sample ◽  
Immunoglobulin G ◽  
Case Definition ◽  
Serological Survey ◽  
Sars Coronavirus ◽  
Antibody Testing ◽  
First Case ◽  
Infectious Stage ◽  
High Infectivity

The first case of severe acute respiratory syndrome (SARS) in France was diagnosed in March 2003. We conducted a serological survey to assess whether or not asymptomatic persons who had been in contact with this patient during his infectious stage had been infected. They were interviewed and asked to provide a blood sample for SARS coronavirus immunoglobulin G antibody testing. Despite the likely high infectivity of the SARS patient, no asymptomatic SARS infection was found in any of the 37 contacts included. These findings support a SARS case definition that is essentially based on clinical and epidemiological assessment, should SARS re-emerge.

Sign in / Sign up

Export Citation Format

Share Document