scholarly journals Absence of infection in asymptomatic contacts of index SARS case in France

2006 ◽  
Vol 11 (1) ◽  
pp. 9-10 ◽  
Author(s):  
S Le Vu ◽  
Y Yazdanpanah ◽  
D Bitar ◽  
J Emmanuelli ◽  
I Bonmarin ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Blood Sample ◽  
Immunoglobulin G ◽  
Case Definition ◽  
Serological Survey ◽  
Sars Coronavirus ◽  
Antibody Testing ◽  
First Case ◽  
Infectious Stage ◽  
High Infectivity

The first case of severe acute respiratory syndrome (SARS) in France was diagnosed in March 2003. We conducted a serological survey to assess whether or not asymptomatic persons who had been in contact with this patient during his infectious stage had been infected. They were interviewed and asked to provide a blood sample for SARS coronavirus immunoglobulin G antibody testing. Despite the likely high infectivity of the SARS patient, no asymptomatic SARS infection was found in any of the 37 contacts included. These findings support a SARS case definition that is essentially based on clinical and epidemiological assessment, should SARS re-emerge.

scholarly journals Specific Immunoglobulin G Antibody Detected in Umbilical Blood and Amniotic Fluid from a Pregnant Woman Infected by the Coronavirus Associated with Severe Acute Respiratory Syndrome

2004 ◽  
Vol 11 (6) ◽  
pp. 1182-1184 ◽  
Author(s):  
Xiugao Jiang ◽  
Xing Gao ◽  
Han Zheng ◽  
Meiying Yan ◽  
Weili Liang ◽  
...  
Keyword(s):  
Pregnant Woman ◽  
Severe Acute Respiratory Syndrome ◽  
Amniotic Fluid ◽  
Immunoglobulin G ◽  
Maternal Blood ◽  
Sars Coronavirus ◽  
Umbilical Blood ◽  
Specific Immunoglobulin

ABSTRACT Specific immunoglobulin G antibody for severe acute respiratory syndrome (SARS) coronavirus was detected in maternal blood, umbilical blood, and amniotic fluid from a pregnant SARS patient. Potential protection of fetus from infection was suggested.

scholarly journals SARS surveillance case definition updated in United States

2003 ◽  
Vol 7 (18) ◽  
Author(s):  
Keyword(s):  
United States ◽  
Severe Acute Respiratory Syndrome ◽  
Laboratory Tests ◽  
Laboratory Data ◽  
The United States ◽  
Case Definition ◽  
Sars Coronavirus ◽  
Serum Samples ◽  
The Us

The surveillance case definition for Severe Acute Respiratory Syndrome (SARS) has been updated for the United States to take account of the results of laboratory tests for SARS-coronavirus (SARS-CoV) that are becoming increasingly available (1) (http://www.cdc.gov/mmwr/preview/mmwrhtml/mm52d429a1.htm). Reported cases of SARS in the US will continue to be classified as suspect or probable; however, these cases can be further classified as laboratory-confirmed or -negative if laboratory data are available and complete, or as laboratory-indeterminate if specimens are not available or testing is incomplete. Obtaining convalescent serum samples to make a final determination about infection with SARS-CoV is critical.

scholarly journals Longitudinal Analysis of Severe Acute Respiratory Syndrome (SARS) Coronavirus-Specific Antibody in SARS Patients

2005 ◽  
Vol 12 (12) ◽  
pp. 1455-1457 ◽  
Author(s):  
Shan-Chwen Chang ◽  
Jann-Tay Wang ◽  
Li-Min Huang ◽  
Yee-Chun Chen ◽  
Chi-Tai Fang ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Longitudinal Analysis ◽  
Specific Antibody ◽  
Immunoglobulin G ◽  
Disease Onset ◽  
Blood Sampling ◽  
Sars Coronavirus ◽  
Serum Antibodies ◽  
Igg Antibody ◽  
Igm Antibody

ABSTRACT The serum antibodies to severe acute respiratory syndrome (SARS) coronavirus of 18 SARS patients were checked at 1 month and every 3 months after disease onset. All of them except one, who missed blood sampling at 1 month, tested positive for the immunoglobulin G (IgG) antibody at 1 month. Fifteen out of 17 tested positive for the IgM antibody at 1 month. The serum IgM antibody of most patients became undetectable within 6 months after the onset of SARS. The IgG antibody of all 17 patients, whose serum was checked 1 year after disease onset, remained positive.

scholarly journals Recombinant Protein-Based Assays for Detection of Antibodies to Severe Acute Respiratory Syndrome Coronavirus Spike and Nucleocapsid Proteins

2007 ◽  
Vol 14 (3) ◽  
pp. 331-333 ◽  
Author(s):  
Lia M. Haynes ◽  
Congrong Miao ◽  
Jennifer L. Harcourt ◽  
Joel M. Montgomery ◽  
Mai Quynh Le ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Recombinant Protein ◽  
Sensitivity And Specificity ◽  
Immunoglobulin G ◽  
High Sensitivity ◽  
Kinetic Studies ◽  
Spike Protein ◽  
Sars Coronavirus ◽  
Nucleocapsid Proteins

ABSTRACT Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

scholarly journals Longitudinal Profile of Immunoglobulin G (IgG), IgM, and IgA Antibodies against the Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein in Patients with Pneumonia Due to the SARS Coronavirus

2004 ◽  
Vol 11 (4) ◽  
pp. 665-668 ◽  
Author(s):  
Patrick C. Y. Woo ◽  
Susanna K. P. Lau ◽  
Beatrice H. L. Wong ◽  
Kwok-hung Chan ◽  
Chung-ming Chu ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Indirect Immunofluorescence ◽  
Immunoglobulin G ◽  
Nucleocapsid Protein ◽  
Baseline Level ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sars Coronavirus ◽  
Iga Antibodies

ABSTRACT By using a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein-based enzyme-linked immunosorbent assay (ELISA) and serum specimens serially collected (from day 0 to day 240 after symptom onset) from patients with pneumonia due to SARS-CoV, we analyzed the longitudinal profiles of immunoglobulin G (IgG), IgM, and IgA antibodies against the SARS-CoV nucleocapsid protein in patients with pneumonia due to SARS-CoV. For IgG, the median optical density at 450 nm (OD450) turned positive at day 17 and a biphasic response was observed. At day 240, all patients were still positive for anti-nucleocapsid protein IgG antibody. For IgM, the median OD450 turned positive at day 20.5, peaked at about day 80, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgM antibody. For IgA, the median OD450 turned positive at day 17, peaked at about day 50, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgA antibody. The time of seroconversion detected by the recombinant SARS-CoV nucleocapsid protein-based ELISA and that detected by indirect immunofluorescence assay were similar. The median times of seroconversion for IgG, IgM, and IgA detected by the indirect immunofluorescence assay were 17 days (17 days by ELISA), 16.5 days (20.5 days by ELISA), and 17.5 days (17 days by ELISA), respectively, after disease onset. One, four, and one of the six patients who died did not produce any IgG, IgM, and IgA antibodies against the nucleocapsid protein of SARS-CoV, respectively, although these antibodies were detected in all six patients by the indirect immunofluorescence assay. Further studies should be performed to see whether SARS-CoV nucleocapsid protein antibody positivity has any prognostic significance.

scholarly journals Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Prevalence in Blood in a Large School Community Subject to a Coronavirus Disease 2019 Outbreak: A Cross-sectional Study

2020 ◽  
Author(s):  
Juan Pablo Torres ◽  
Cecilia Piñera ◽  
Verónica De La Maza ◽  
Anne J Lagomarcino ◽  
Daniela Simian ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
School Community ◽  
Antibody Detection ◽  
Lower Grade ◽  
Rt Pcr ◽  
Antibody Testing ◽  
Cross Sectional ◽  
Large School ◽  
First Case ◽  
Antibody Positivity

Abstract Background A severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak affecting 52 people from a large school community in Santiago, Chile, was identified (12 March) 9 days after the first case in the country. We assessed the magnitude of the outbreak and the role students and staff played using self-administered antibody detection tests and a self-administered survey. Methods The school was closed on 13 March, and the entire community was placed under quarantine. We implemented a home-delivery, self-administered, immunoglobin (Ig) G/IgM antibody test and survey to a classroom-stratified sample of students and all staff from 4–19 May. We aimed to determine the overall seroprevalence rates by age group, reported symptoms, and contact exposure, and to explore the dynamics of transmission. Results The antibody positivity rates were 9.9% (95% confidence interval [CI], 8.2–11.8) for 1009 students and 16.6% (95% CI, 12.1–21.9) for 235 staff. Among students, positivity was associated with a younger age (P = .01), a lower grade level (P = .05), prior real-time polymerase chain reaction (RT-PCR) positivity (P = .03), and a history of contact with a confirmed case (P < .001). Among staff, positivity was higher in teachers (P = .01) and in those previously RT-PCR positive (P < .001). Excluding RT-PCR–positive individuals, antibody positivity was associated with fever in adults and children (P = .02 and P = .002, respectively), abdominal pain in children (P = .001), and chest pain in adults (P = .02). Within antibody-positive individuals, 40% of students and 18% of staff reported no symptoms (P = .01). Conclusions Teachers were more affected during the outbreak and younger children were at a higher risk for infection, likely because index case(s) were teachers and/or parents from the preschool. Self-administered antibody testing, supervised remotely, proved to be a suitable and rapid tool. Our study provides useful information for school reopenings.

scholarly journals Seroprevalence of anti–SARS-CoV-2 IgG antibodies in Kenyan blood donors

Science ◽  
2020 ◽  
Vol 371 (6524) ◽  
pp. 79-82 ◽  
Author(s):  
Sophie Uyoga ◽  
Ifedayo M. O. Adetifa ◽  
Henry K. Karanja ◽  
James Nyagwange ◽  
James Tuju ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Confidence Interval ◽  
Immunoglobulin G ◽  
Test Performance ◽  
Blood Donors ◽  
Igg Antibodies ◽  
First Case ◽  
Pandemic Response ◽  
Case Based

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Africa is poorly described. The first case of SARS-CoV-2 in Kenya was reported on 12 March 2020, and an overwhelming number of cases and deaths were expected, but by 31 July 2020, there were only 20,636 cases and 341 deaths. However, the extent of SARS-CoV-2 exposure in the community remains unknown. We determined the prevalence of anti–SARS-CoV-2 immunoglobulin G among blood donors in Kenya in April–June 2020. Crude seroprevalence was 5.6% (174 of 3098). Population-weighted, test-performance-adjusted national seroprevalence was 4.3% (95% confidence interval, 2.9 to 5.8%) and was highest in urban counties Mombasa (8.0%), Nairobi (7.3%), and Kisumu (5.5%). SARS-CoV-2 exposure is more extensive than indicated by case-based surveillance, and these results will help guide the pandemic response in Kenya and across Africa.

scholarly journals Kinetics of Severe Acute Respiratory Syndrome (SARS) Coronavirus-Specific Antibodies in 271 Laboratory-Confirmed Cases of SARS

2004 ◽  
Vol 11 (4) ◽  
pp. 792-794 ◽  
Author(s):  
Zhongping He ◽  
Qingming Dong ◽  
Hui Zhuang ◽  
Shujing Song ◽  
Guoai Peng ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Enzyme Immunoassay ◽  
Immunoglobulin G ◽  
Immunofluorescence Assay ◽  
Sars Coronavirus ◽  
Specific Antibodies ◽  
Kinetics Of

ABSTRACT The sensitivities and specificities of an immunofluorescence assay and an enzyme immunoassay for detection of antibodies specific for severe acute respiratory syndrome coronavirus (SARS-CoV) were compared for 148 laboratory-confirmed SARS cases. The appearance and persistence of SARS-CoV-specific antibodies were assessed, with immunoglobulin G detected in 59% of samples collected within 14 days and persisting for 60 to 95 days after the onset of illness.

Novel Coronavirus Disease (COVID-19): A Narrative Review Based on Current Status

2020 ◽  
Vol 18 ◽  
Author(s):  
Rina Das ◽  
Dinesh Kumar Mehta ◽  
Meenakshi Dhanawat
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Clinical Symptoms ◽  
Virus Disease ◽  
Relevant Literature ◽  
World Health ◽  
Current Status ◽  
Future Research ◽  
Treatment And Prevention ◽  
First Case ◽  
Novel Coronavirus

Abstract:: A novel virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), appeared and expanded globally by the end of year in 2019 from Wuhan, China, causing severe acute respiratory syndrome. During its initial stage, the disease was called the novel coronavirus (2019-nCoV). It was named COVID-19 by the World Health Organization (WHO) on 11 February 2020. The WHO declared worldwide the SARS-CoV-2 virus a pandemic on March 2020. On 30 January 2020 the first case of Corona Virus Disease 2019 (COVID-19) was reported in India. Now in current situation the virus is floating in almost every part of the province and rest of the globe. -: On the basis of novel published evidences, we efficiently summarized the reported work with reference to COVID-19 epidemiology, pathogen, clinical symptoms, treatment and prevention. Using several worldwide electronic scientific databases such as Pubmed, Medline, Embase, Science direct, Scopus, etc were utilized for extensive investigation of relevant literature. -: This review is written in the hope of encouraging the people successfully with the key learning points from the underway efforts to perceive and manage SARS-CoV-2, suggesting sailent points for expanding future research.

scholarly journals SARS-CoV-2 Seroprevalence among Healthcare Workers in General Hospitals and Clinics in Japan

2021 ◽  
Vol 18 (7) ◽  
pp. 3786
Author(s):  
Tatsuya Yoshihara ◽  
Kazuya Ito ◽  
Masayoshi Zaitsu ◽  
Eunhee Chung ◽  
Izumi Aoyagi ◽  
...  
Keyword(s):  
Public Health ◽  
Social Support ◽  
General Population ◽  
Severe Acute Respiratory Syndrome ◽  
Immunoglobulin G ◽  
Health Problem ◽  
Healthcare Workers ◽  
Public Health Problem ◽  
General Hospitals ◽  
Second Wave

Coronavirus disease 2019 (COVID-19) has become a serious public health problem worldwide. In general, healthcare workers are considered to be at higher risk of COVID-19 infection. However, the prevalence of COVID-19 among healthcare workers in Japan is not well characterized. In this study, we aimed to examine the seroprevalence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies among 2160 healthcare workers in hospitals and clinics that are not designated to treat COVID-19 patients in Japan. The prevalence of SARS-CoV-2 immunoglobulin G was 1.2% in August and October 2020 (during and after the second wave of the pandemic in Japan), which is relatively higher than that in the general population in Japan (0.03–0.91%). Because of the higher risk of COVID-19 infection, healthcare workers should be the top priority for further social support and vaccination against SARS-CoV-2.

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