scholarly journals Genome Sequence of theListeria monocytogenesFood Isolate HPB913, Collected in Canada in 1993

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Arthur W. Pightling ◽  
Hugh Rand ◽  
Errol Strain ◽  
Franco Pagotto

Listeria monocytogenesis a pathogenic bacterium of importance to public health and food safety agencies. We present the genome sequence of the serotype 1/2aL. monocytogenesfood isolate HPB913, which was collected in Canada in 1993 as part of an investigation into a sporadic case of foodborne illness.

2017 ◽  
Vol 47 (2) ◽  
Author(s):  
Carla Susana Rodrigues ◽  
Cláudia Valéria Gonçalves Cordeiro de Sá ◽  
Cristiano Barros de Melo

ABSTRACT: Listeria monocytogenes is a relevant foodborne pathogen in public health, responsible for outbreaks of listeriosis often associated to the consumption of ready to eat meat, dairy and fishery products. Listeriosis is a serious disease that can lead to death and mainly affect children, the elderly and immunocompromised individuals. In pregnant women causes abortion or neonatal listeriosis. In Brazil, ready to eat food are appreciated and increasingly consumed by the population. Furthermore, products such as sausages, bologna, hams and cheeses have characteristics such as pH, Aw and sodium chloride content that favor the development of L. monocytogenes during their shelf life. The purpose of this paper was to present an overview of L. monocytogenes contamination in different meat, dairy and fishery products that are ready for consumption and thereby support the adoption of strategies to mitigate this risk, contributing to achieve the appropriate level protection for the consumers and thus strengthen Brazil's food safety system.


2019 ◽  
Vol 10 (1) ◽  
pp. 20190046 ◽  
Author(s):  
Alison Stout ◽  
Anna Van Stelten-Carlson ◽  
Hélène Marquis ◽  
Michael Ballou ◽  
Brian Reilly ◽  
...  

Listeriosis is a clinically severe foodborne disease caused by Listeria monocytogenes (Lm). However, approximately 45% of Lm isolates in food carry a virulence-attenuating single-nucleotide polymorphism in inlA , which normally facilitates crossing the intestinal barrier during the initial stages of infection. We hypothesized that (i) natural exposure to virulence-attenuated (vA) Lm strains through food can confer protective immunity against listeriosis attributable to fully virulent (fV) strains and (ii) current food safety measures to minimize exposure to both Lm strains may have adverse population-level outcomes. To test these hypotheses, we evaluated the host response to Lm in a mouse infection model and through mathematical modelling in a human population. After oral immunization with a murinized vA Lm strain, we demonstrated the elicitation of a CD8+ T-cell response and protection against subsequent challenge with an fV strain. A two-strain compartmental mathematical model of human exposure to Lm with cross-protective immunity was also developed. If food safety testing strategies preferentially identify and remove food contaminated by vA strains (potentially due to their common occurrence in foods and higher concentration in food compared to fV strains), the model predicted minimal public health benefit to potentially adverse effects. For example, reducing vA exposures by half, while maintaining fV exposures results in an approximately 6% rise in annual incidence.


2017 ◽  
Vol 5 (49) ◽  
Author(s):  
Taylor W. Bailey ◽  
Naila C. do Nascimento ◽  
Arun K. Bhunia

ABSTRACT Listeria monocytogenes is an opportunistic invasive foodborne pathogen. Here, we performed whole-genome sequencing of L. monocytogenes strain F4244 (serotype 4b) using Illumina sequencing. The sequence showed 94.5% identity with strain F2365, serotype 4b, and 90.6% with EGD-e, serotype 1/2a.


2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Arthur W. Pightling ◽  
Hugh Rand ◽  
Errol Strain ◽  
Franco Pagotto

Listeria monocytogenesis a foodborne pathogen that causes severe illness. Thus, ongoing efforts at real-time whole-genome sequencing are of utmost importance. However, it is also important that retrospective analyses that place these data into context be performed. Here, we present the genome sequence of strain HPB2088, which was collected in 1994.


2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Aidan Casey ◽  
Olivia McAuliffe ◽  
Aidan Coffey ◽  
Karen Hunt ◽  
Seamus Fanning ◽  
...  

2020 ◽  
Vol 70 (4) ◽  
pp. 1859
Author(s):  
S. SAHIN ◽  
R. KALIN ◽  
MN MOGULKOC

Listeria monocytogenes is one of the important causes of food-borne infections. This study was conducted to determine the presence of L. monocytogenes and its serotype distribution in a total of 400 packaged chicken meat products (drumstick, breast, wing, and whole chicken) from different national companies. L. monocytogenes contamination was detected in 26.5% (106 in 400) of all samples when the products considered, drumsticks, breasts, wings, and whole chickens showed 47%, 15%, 35, and 9% positivity respectively. Four important serotypes of L. monocytogenes in human listeriosis (1/2a, 1/2b, 1/2c and 4b) were identified, and serotype 1/2a (94.3%) was determined as predominant in packaged chicken meats. The present study revealed that L. monocytogenes 1/2a serotype is prevalent in chicken meats and this may cause public health problems in Turkey. Further studies in poultry meats should be conducted on a large scale such as regional or national big markets to determine the presence of the pathogen and its dominant serotypes.


2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Sripriya Rajamani ◽  
Melanie Firestone ◽  
Craig Hedberg

Foodborne illnesses remain an important public health challenge in the United States causing an estimated 48 million illnesses, 128,000 hospitalizations, and 3,000 deaths per year. Restaurants are frequent settings for foodborne illness transmission. Public health surveillance – the continual, systematic collection, analysis, and interpretation of reports of health data to prevent and control illness – is a prerequisite for an effective food control system. While restaurant inspection data are routinely collected, these data are not regularly aggregated like traditional surveillance data. However, there is evidence that these data are a valuable tool for understanding foodborne illness outbreaks and threats to food safety. This article discusses the challenges and opportunities for incorporating routine restaurant inspection data as a surveillance tool for monitoring and improving foodborne illness prevention activities.  The three main challenges are: 1) the need for a national framework; 2) lack of data standards and interoperability; and 3) limited access to restaurant inspection data. Tapping into the power of public health informatics represents an opportunity to address these challenges. Overall, improving restaurant inspection information systems and making restaurant inspection data available to support decision-making represents an opportunity to practice smarter food safety.


2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Michael J. Rothrock ◽  
Peixin Fan ◽  
Kwangcheol C. Jeong ◽  
Sun Ae Kim ◽  
Steven C. Ricke ◽  
...  

ABSTRACT Investigation of Listeria monocytogenes transmission from environmental sources associated with pasture-raised chickens to poultry products is needed to determine ways to prevent potential foodborne illness. Here, we report the complete genome sequence of Listeria monocytogenes MR310, one of the isolates from a pastured-flock poultry management system.


2003 ◽  
Vol 66 (2) ◽  
pp. 256-264 ◽  
Author(s):  
E. A. SZABO ◽  
L. SIMONS ◽  
M. J. COVENTRY ◽  
M. B. COLE

The important new concept of the food safety objective (FSO) offers a strategy to translate public health risk into a definable goal such as a specified maximum frequency or concentration of a hazardous agent in a food at the time of consumption that is deemed to provide an appropriate level of health protection. For the foodborne pathogen Listeria monocytogenes, there is a proposed FSO of <100 CFU/g in ready-to-eat (RTE) products at the time of consumption. Fresh precut iceberg lettuce is one of these RTE products. In this study, we worked with a commercial manufacturer to evaluate the effectiveness of two antimicrobial washing agents (sodium hypochlorite and a mixture of hydrogen peroxide and peroxyacetic acid) against L. monocytogenes under simulated fresh precut washing conditions and evaluated the growth potential of this pathogen on lettuce packaged in a gas-permeable film and stored at 4 or 8°C for 14 days. We used the results of this experiment to demonstrate how the commercial manufacturer could meet the FSO for L. monocytogenes in fresh precut lettuce through the application of performance, process, and microbiological criteria.


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