scholarly journals Distribution of serotypes of Listeria monocytogenes in chicken meats in Turkey

2020 ◽  
Vol 70 (4) ◽  
pp. 1859
Author(s):  
S. SAHIN ◽  
R. KALIN ◽  
MN MOGULKOC
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Large Scale ◽  
Meat Products ◽  
Health Problems ◽  
Chicken Meat ◽  
Serotype Distribution ◽  
Food Borne ◽  
Serotype 1 ◽  
Food Borne Infections

Listeria monocytogenes is one of the important causes of food-borne infections. This study was conducted to determine the presence of L. monocytogenes and its serotype distribution in a total of 400 packaged chicken meat products (drumstick, breast, wing, and whole chicken) from different national companies. L. monocytogenes contamination was detected in 26.5% (106 in 400) of all samples when the products considered, drumsticks, breasts, wings, and whole chickens showed 47%, 15%, 35, and 9% positivity respectively. Four important serotypes of L. monocytogenes in human listeriosis (1/2a, 1/2b, 1/2c and 4b) were identified, and serotype 1/2a (94.3%) was determined as predominant in packaged chicken meats. The present study revealed that L. monocytogenes 1/2a serotype is prevalent in chicken meats and this may cause public health problems in Turkey. Further studies in poultry meats should be conducted on a large scale such as regional or national big markets to determine the presence of the pathogen and its dominant serotypes.

Biofilm Formation and Associated Genes in Listeria Monocytogenes

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
S. R. Warke ◽  
V. C. Ingle ◽  
N. V. Kurkure ◽  
P. A. Tembhurne ◽  
Minakshi Prasad ◽  
...  
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Food Processing ◽  
Biofilm Formation ◽  
Milk Samples ◽  
Biofilm Production ◽  
Food Borne ◽  
Serious Infections ◽  
Microtiter Assay

Listeria monocytogenes, an opportunistic food borne pathogen can cause serious infections in immunocompromised individuals. L. monocytogenes is capable of producing biofilm on the surface of food processing lines and instruments.The biofilm transfers contamination to food products and impose risk to public health. In the present study biofilm producing ability of L. monocytogenes isolates were investigated phenotypically and genotypically by microtiter assay and multiplex PCR, respectively. Out of 38 L. monocytogenes isolates 14 were recovered from animal clinical cases, 12 bovine environment and 12 from milk samples. A total of 3 (21.42%) clinical, 2 (16.66%) environment and 3 (25%) milk samples respectively, revealed biofilm production in microtiter assay. Cumulative results showed that 23 (60.52%) out of 38 strains of L. monocytogenes were positive for luxS and flaA gene and 1 (2.63%) was positive only for the flaA gene.

TOTAL VOLUME OF MEDICAL CARE FOR CHILDREN BASED ON AN ANALYSIS OF EIGHT STATES

PEDIATRICS ◽  
1948 ◽  
Vol 1 (2) ◽  
pp. 227-236
Author(s):  
WARREN R. SISSON ◽  
KATHERINE BAIN ◽  
ROLLO H. BRITTEN ◽  
MARYLAND Y. PENNELL ◽  
JOHN P. HUBBARD
Keyword(s):  
Public Health ◽  
Health Service ◽  
Large Scale ◽  
Public Health Service ◽  
Health Problems ◽  
Federal Agencies ◽  
Child Health Services ◽  
Executive Staff ◽  
Intimate Knowledge ◽  
Statistical Studies

AN ASSIGNMENT has been given to me to present the first official results of the Study of Child Health Services, instituted by the Academy and executed by the Academy with the cooperation of the U. S. Public Health Service and the U. S. Children's Bureau. The paper which I am to read represents the results of work by you and compiled by your Executive Staff of the Study. One would like to conjecture how the future historian of the Academy will evaluate the transactions of this afternoon's session. Certainly this meeting marks a milestone in that it represents an awareness of public health problems, especially those of children, which have never before been so intimately studied. Furthermore, it is noteworthy because of the actual achievement of an investigation of a scope never previously paralleled by a group of physicians. Also the Study has revealed that, with common objectives, groups of physicians in private practice and those from the ranks of academic circles, along with personnel from established governmental bureaus, can work effectively together. It should be reiterated that the Study could not have been accomplished by us as a group of physicians alone nor by any existing governmental department. The two cooperating federal agencies, the U. S. Public Health Service and the U. S. Children's Bureau, have trained personnel with interest and experience in public health problems and in the conduct of large-scale statistical studies. The Academy, on its part, has first-hand experience in medical service and an intimate knowledge of those who are rendering service to children, and through its various members has been able to impart knowledge which otherwise would not have been available.

scholarly journals The importance of an early alert from the Microbiology Laboratory and multidisciplinary collaboration during a suspected salmonellosis outbreak

2017 ◽  
Vol 32 (1) ◽  
Author(s):  
Adriano Anesi ◽  
Maria Lucia Panceri ◽  
Sara Asticcioli ◽  
Dominga Baroni ◽  
Vanina Rognoni ◽  
...  
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Food Industry ◽  
Large Scale ◽  
Regulatory Agencies ◽  
Local Health ◽  
Microbiology Laboratory ◽  
Food Borne ◽  
Local Health Service ◽  
Sporadic Cases

<em>Background and aims.</em> Salmonellosis is one of the most common and widely distributed food-borne diseases. The increasing complexity and globalization of the food industry are causing an increase of some of these large-scale food-borne illnesses, thus there is a need for improvements in public health signal detection and communication streams between laboratories and regulatory agencies. The aim of this study is to show how the early reporting of salmonellosis cases directly from the Laboratory of Microbiology to the Local Health Service Infectious Diseases Office along with the prompt response of the ASL, and the rapid involvement of the Local Veterinary Prevention Department resulted in an improved individuation and investigation of a suspected food-borne outbreak with anomalous manifestation. <br /><em>Materials and methods.</em> From August to November 2014 the early warning from the Laboratory of Microbiology regarding <em>Salmonella</em> spp. isolates with the identical serogroup and antibiotic resistance phenotype, allowed for prompt identification of a food-borne infection. <br /><em>Results and conclusions.</em> The genotyping analysis suggested that over the period considered there was more than a single monophasic <em>Salmonella</em> <em>typhimurium</em> isolate: one responsible for the sporadic cases that occurred in September and October, and another in November.

scholarly journals Pathogenicity, Epidemiology and Virulence Factors of Salmonella species: A Review

2017 ◽  
Vol 9 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Tamègnon Victorien DOUGNON ◽  
Boris LEGBA ◽  
Esther DEGUENON ◽  
Gildas HOUNMANOU ◽  
Jerrold AGBANKPE ◽  
...  
Keyword(s):  
Public Health ◽  
Virulence Factors ◽  
Meat Products ◽  
Health Problems ◽  
Molecular Techniques ◽  
Salmonella Spp ◽  
Salmonella Infections ◽  
The Family ◽  
The World ◽  
Salmonella Control

Salmonella infections are major public health problems worldwide. The hereby review aimed to establish an overview on the pathogenicity, epidemiology and virulence factors of Salmonella spp. in the world. A systematic search was conducted online using the keywords ‘Salmonella’, ‘Salmonella spp.’, ‘Salmonella spp. Epidemiology’, ‘virulence factors of Salmonella spp. in the world’, ‘bacteria responsible for the contamination of meat products’, ‘non-typhoid salmonella’. These keywords were entered into databases such as PubMed and Google Scholar using mainly French language. The obtained articles were included based on the reliability of their source, the study area (usually Benin and Africa) and the subject. The review revealed that Salmonella spp. is motile Gram-negative rod-shaped bacteria, of the family Enterobacteriaceae, currently counting more than 2,600 serovars. Human contamination occurs through the ingestion of contaminated water and food and can cause gastroenteritis or typhoid fever, which are two serious public health problems. A gene set constituting the pathogenicity islands determines the pathogenesis of Salmonella spp. The diagnosis is based on bacteriological, serological and molecular techniques. Salmonella infections are usually treated using antibiotics; however, emergence of antibiotic resistance in these microorganisms suggests that the anti-salmonella control should explore new sources such as medicinal plants

scholarly journals Genome Sequence of theListeria monocytogenesFood Isolate HPB913, Collected in Canada in 1993

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Arthur W. Pightling ◽  
Hugh Rand ◽  
Errol Strain ◽  
Franco Pagotto
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Food Safety ◽  
Genome Sequence ◽  
Sporadic Case ◽  
Foodborne Illness ◽  
Pathogenic Bacterium ◽  
Food Isolate ◽  
Serotype 1

Listeria monocytogenesis a pathogenic bacterium of importance to public health and food safety agencies. We present the genome sequence of the serotype 1/2aL. monocytogenesfood isolate HPB913, which was collected in Canada in 1993 as part of an investigation into a sporadic case of foodborne illness.

scholarly journals Conservation of Genomic Localization and Sequence Content of Sau3AI-Like Restriction-Modification Gene Cassettes among Listeria monocytogenes Epidemic Clone I and Selected Strains of Serotype 1/2a

2010 ◽  
Vol 76 (16) ◽  
pp. 5577-5584 ◽  
Author(s):  
Suleyman Yildirim ◽  
Driss Elhanafi ◽  
Wen Lin ◽  
Anthony D. Hitchins ◽  
Robin M. Siletzky ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gene Cassette ◽  
Conclusive Evidence ◽  
Gene Encoding ◽  
Epidemic Clone ◽  
Food Borne ◽  
Serotype 1 ◽  
Serotype 4B ◽  
Clonal Population Structure ◽  
Restriction Modification

ABSTRACTListeria monocytogenesis a food-borne pathogen with a clonal population structure and apparently limited gene flow between strains of different lineages. Strains of epidemic clone I (ECI) have been responsible for numerous outbreaks and invariably have DNA that is resistant to digestion by Sau3AI, suggesting methylation of cytosine at GATC sites. A putative restriction-modification (RM) gene cassette has been identified in the genome of the ECI strain F2365 and all other tested ECI strains but is absent from other strains of the same serotype (4b). Homologous RM cassettes have not been reported amongL. monocytogenesisolates of other serotypes. Furthermore, conclusive evidence for the involvement of this RM cassette in the Sau3AI resistance phenotype of ECI strains has been lacking. In this study, we describe a highly conserved RM cassette in certain strains of serotypes 1/2a and 4a that have Sau3AI-resistant DNA. In these strains the RM cassette was in the same genomic location as in the ECI reference strain F2365. The cassette included a gene encoding a putative recombinase, suggesting insertion via site-specific recombination. Deletion of the RM cassette in the ECI strain F2365 and the serotype 1/2a strain A7 rendered the DNA of both strains susceptible to Sau3AI digestion, providing conclusive evidence that the cassette includes a gene required for methylation of cytosine at GATC sites in both strains. The findings suggest that, in addition to its presence in ECI strains, this RM cassette and the accompanying genomic DNA methylation is also encountered among selected strains of other lineages.

scholarly journals Physical and antibiotic stresses require activation of the RsbU phosphatase to induce the general stress response in Listeria monocytogenes

Microbiology ◽  
2010 ◽  
Vol 156 (9) ◽  
pp. 2660-2669 ◽  
Author(s):  
Ji-Hyun Shin ◽  
Margaret S. Brody ◽  
Chester W. Price
Keyword(s):  
Listeria Monocytogenes ◽  
Protein Interactions ◽  
Single Copy ◽  
Nutritional Stress ◽  
Serine Phosphorylation ◽  
Intestinal Lumen ◽  
Food Borne ◽  
Multiple Challenges ◽  
Stress Signals ◽  
Food Borne Infections

Among pathogenic strains of Listeria monocytogenes, the σ B transcription factor has a pivotal role in the outcome of food-borne infections. This factor is activated by diverse stresses to provide general protection against multiple challenges, including those encountered during gastrointestinal passage. It also acts with the PrfA regulator to control virulence genes needed for entry into intestinal lumen cells. Environmental and nutritional signals modulate σ B activity via a network that operates by the partner switching mechanism, in which protein interactions are controlled by serine phosphorylation. This network is well characterized in the related bacterium Bacillus subtilis. A key difference in Listeria is the presence of only one input phosphatase, RsbU, instead of the two found in B. subtilis. Here, we aim to determine whether this sole phosphatase is required to convey physical, antibiotic and nutritional stress signals, or if additional pathways might exist. To that end, we constructed L. monocytogenes 10403S strains bearing single-copy, σ B-dependent opuCA–lacZ reporter fusions to determine the effects of an rsbU deletion under physiological conditions. All stresses tested, including acid, antibiotic, cold, ethanol, heat, osmotic and nutritional challenge, required RsbU to activate σ B. This was of particular significance for cold stress activation, which occurs via a phosphatase-independent mechanism in B. subtilis. We also assayed the effects of the D80N substitution in the upstream RsbT regulator that activates RsbU. The mutant had a phenotype consistent with low and uninducible phosphatase activity, but nonetheless responded to nutritional stress. We infer that RsbU activity but not its induction is required for nutritional signalling, which would enter the network downstream from RsbU.

scholarly journals Comparative Proteomic Analysis of Listeria monocytogenes Strains F2365 and EGD

2008 ◽  
Vol 75 (2) ◽  
pp. 366-373 ◽  
Author(s):  
Janet R. Donaldson ◽  
Bindu Nanduri ◽  
Shane C. Burgess ◽  
Mark L. Lawrence
Keyword(s):  
Listeria Monocytogenes ◽  
Protein Identification ◽  
Altered Expression ◽  
Genetic Lineages ◽  
Multidimensional Protein Identification Technology ◽  
Food Borne ◽  
Flagellar Biosynthesis ◽  
Serotype 1 ◽  
Serotype 4B ◽  
Biological Differences

ABSTRACT Listeria monocytogenes is a gram-positive, food-borne pathogen that causes disease in both humans and animals. There are three major genetic lineages of L. monocytogenes and 13 serovars. To further our understanding of the differences that exist between different genetic lineages/serovars of L. monocytogenes, we analyzed the global protein expression of the serotype 1/2a strain EGD and the serotype 4b strain F2365 during early-stationary-phase growth at 37°C. Using multidimensional protein identification technology with electrospray ionization tandem mass spectrometry, we identified 1,754 proteins from EGD and 1,427 proteins from F2365, of which 1,077 were common to both. Analysis of proteins that had significantly altered expression between strains revealed potential biological differences between these two L. monocytogenes strains. In particular, the strains differed in expression of proteins involved in cell wall physiology and flagellar biosynthesis, as well as DNA repair proteins and stress response proteins.

scholarly journals Genetic Diversity of Listeria monocytogenes Strains from a High-Prevalence Dairy Farm

2005 ◽  
Vol 71 (10) ◽  
pp. 5893-5899 ◽  
Author(s):  
Monica K. Borucki ◽  
Clive C. Gay ◽  
James Reynolds ◽  
Katherine L. McElwain ◽  
So Hyun Kim ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Pacific Northwest ◽  
Dairy Farm ◽  
Clinical Strain ◽  
Time Points ◽  
Source Of Infection ◽  
Food Borne ◽  
High Prevalence ◽  
Serotype 1 ◽  
On Farm

ABSTRACT Listeria monocytogenes is a significant food-borne human and veterinary pathogen. Contaminated silage commonly leads to disease in livestock, but the pervasive nature of the bacterium can make it difficult to identify the source of infection. An investigation of bovine listeriosis that occurred on a Pacific Northwest dairy farm (“farm A”) revealed that the clinical strain was closely related to fecal strains from asymptomatic cows, and that farm environment was heavily contaminated with a diversity of L. monocytogenes strains. In addition, the farm A clinical strain was closely related to clinical and environmental strains obtained 1 year prior from a second Northwest dairy farm (“farm B”). To investigate the source(s) of contamination on farm A, environmental samples were collected from farm A at two time points. Pulsed-field gel electrophoresis characterization of 538 isolates obtained from that farm identified 57 different AscI pulsovars. Fecal isolates obtained from individual cows were the most genetically diverse, with up to 94% of fecal samples containing more than one pulsovar. The maximum numbers of pulsovars and serotypes isolated from a fecal sample of one cow were 6 and 4, respectively. Serotype 1/2a was isolated most frequently at both time points. Microarray genotyping of bovine listeriosis, fecal, and silage strains from both farms identified four probes that differentiated listeriosis strains from environmental strains; however, no probe was common to both bovine listeriosis strains.

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