scholarly journals Draft Genome Sequences of Five Shiga Toxin-Producing Escherichia coli Isolates Harboring the New and Recently Described Subtilase Cytotoxin Allelic Variant subAB2-3

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Taurai Tasara ◽  
Lisa Fierz ◽  
Jochen Klumpp ◽  
Herbert Schmidt ◽  
Roger Stephan
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Sequence Data ◽  
Draft Genome ◽  
Allelic Variant ◽  
Genome Sequences ◽  
Content Type ◽  
Subtilase Cytotoxin

ABSTRACT We present here the draft genome sequences of five Shiga toxin-producing Escherichia coli (STEC) strains which tested positive in a primary subAB screening. Assembly and annotation of the draft genomes revealed that all strains harbored the recently described allelic variant subAB 2-3 . Based on the sequence data, primers were designed to identify and differentiate this variant.

scholarly journals Complete Genome Sequence of a Shiga Toxin-Producing Escherichia coli O26:H11 Strain (Sequence Type 21) and Two Draft Genome Sequences of Listeria monocytogenes Strains (Clonal Complex 1 [CC1] and CC59) Isolated from Fresh Produce in Germany

2020 ◽  
Vol 9 (49) ◽  
Author(s):  
Gregor Fiedler ◽  
Jan Kabisch ◽  
Erik Brinks ◽  
Sabrina Sprotte ◽  
Christina Boehnlein ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Shiga Toxin ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Clonal Complex ◽  
Draft Genome ◽  
Sequence Type ◽  
Genome Sequences ◽  
Content Type

ABSTRACT The complete genome sequence of a Shiga toxin-producing Escherichia coli (STEC) O26:H11 strain, MBT-5 (sequence type 21 [ST21], stx1a, stx2a, eae, ehxA), and two draft genome sequences of Listeria monocytogenes strains MBT-6 and MBT-7 belonging to the virulent sequence types 1 (ST1, clonal complex 1 [CC1]) and 59 (ST59, CC59), respectively, were determined. The strains were isolated in 2015 from ready-to-eat mixed greens in Germany.

scholarly journals Draft Genome Sequences of Shiga Toxin-Producing Escherichia coli O157:H7 Strains Recovered from a Major Production Region for Leafy Greens in California

2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Beatriz Quiñones ◽  
Jaszemyn C. Yambao ◽  
Christopher J. Silva ◽  
Bertram G. Lee
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Draft Genome ◽  
Foodborne Pathogen ◽  
Toxin Gene ◽  
Genome Sequences ◽  
Content Type ◽  
Production Region ◽  
Leafy Greens ◽  
Different Levels

ABSTRACT Shiga toxin-producing Escherichia coli O157:H7 is a foodborne pathogen and is responsible for outbreaks of human gastroenteritis. This report documents the draft genome sequences of nine O157:H7 cattle strains, which were identified to be PCR positive for a Shiga toxin gene but displayed different levels of functional toxin activity.

scholarly journals Draft Genome Sequences of 12 Shiga Toxin-Producing Escherichia coli Strains Isolated from Dairy Cattle in Portugal

2020 ◽  
Vol 9 (38) ◽  
Author(s):  
Gregor Fiedler ◽  
Andressa Ballem ◽  
Erik Brinks ◽  
Carina Almeida ◽  
Charles M. A. P. Franz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Dairy Cattle ◽  
Shiga Toxin ◽  
Draft Genome ◽  
Foodborne Pathogen ◽  
Genome Sequences ◽  
Content Type ◽  
The North ◽  
Contaminated Food

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen transmitted from animals to humans through contaminated food. Cattle are the main reservoir of STEC, but their genetic diversity is still poorly characterized, especially regarding strains isolated in Portugal. We therefore present the draft genomic sequences of 12 STEC strains isolated from cattle in the north of Portugal.

scholarly journals Draft Genome Sequences of Seven Strains of Shiga Toxin-Producing Escherichia coli O111 with Variation in Their Sensitivity to Novobiocin

2018 ◽  
Vol 7 (10) ◽  
Author(s):  
Luca Rotundo ◽  
Federica Boccia ◽  
Pina M. Fratamico ◽  
Aixia Xu ◽  
Christopher H. Sommers ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Draft Genome ◽  
False Negative ◽  
Genomic Sequences ◽  
Selective Agent ◽  
Genome Sequences ◽  
Content Type ◽  
Negative Detection

Inclusion of novobiocin as a selective agent for enrichment media and selective agars inhibits the growth of some Shiga toxin-producing Escherichia coli (STEC) strains, particularly non-O157 STEC, which can yield false-negative detection results. Here, we report the draft genomic sequences of seven STEC O111 isolates with different sensitivities to novobiocin.

scholarly journals Draft Genome Sequences of Escherichia coli O113:H21 Strains Recovered from a Major Produce Production Region in California

2017 ◽  
Vol 5 (44) ◽  
Author(s):  
Beatriz Quiñones ◽  
Jaszemyn C. Yambao ◽  
Bertram G. Lee
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Draft Genome ◽  
Soil Samples ◽  
Genome Sequences ◽  
Content Type ◽  
Agricultural Region ◽  
Production Region ◽  
Waterborne Pathogen ◽  
Leafy Greens

ABSTRACT Shiga toxin-producing Escherichia coli is a foodborne and waterborne pathogen and is responsible for outbreaks of human gastroenteritis. This report documents the draft genome sequences of seven O113:H21 strains recovered from livestock, wildlife, and soil samples recovered from a major agricultural region for leafy greens in California, USA.

scholarly journals Genome Sequences of Uncommon Shiga Toxin-Producing Escherichia coli Serotypes

2020 ◽  
Vol 9 (10) ◽  
Author(s):  
Baha Abdalhamid ◽  
Emily L. Mccutchen ◽  
Peter C. Iwen ◽  
Joao Carlos Gomes-Neto ◽  
Andrew K. Benson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Draft Genome ◽  
The Other ◽  
Foodborne Disease ◽  
Genome Sequences ◽  
Content Type

Shiga toxin-producing Escherichia coli (STEC) is a foodborne disease with worldwide outbreaks. STEC serotypes O157, O26, O45, O103, O111, O121, and O145 cause the most outbreaks. There is little published information regarding the other serotypes. We report the draft genome sequences for 11 uncommon STEC serotypes from Nebraska.

scholarly journals Draft Genome Sequences of 19 Clinical stx -Harboring Escherichia coli O80:H2 Strains

2021 ◽  
Vol 10 (11) ◽  
Author(s):  
Marc J. A. Stevens ◽  
Nicole Cernela ◽  
Andrea Müller ◽  
Magdalena Nüesch-Inderbinen ◽  
Roger Stephan
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Draft Genome ◽  
Genome Sequences ◽  
Content Type

Shiga toxin-producing Escherichia coli (STEC) O80:H2 is an uncommon hybrid pathotype that has emerged in Switzerland and France. Here, we report the draft genome sequences of 19 stx -harboring Escherichia coli O80:H2 strains isolated between 2003 and 2019 from patients in Switzerland.

scholarly journals Draft Genome Sequences of Escherichia coli Strains FP2 and FP3, Isolated from the Canada Goose (Branta canadensis)

2018 ◽  
Vol 7 (9) ◽  
Author(s):  
Allison L. Denny ◽  
Susan E. Arruda
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Branta Canadensis ◽  
Canada Goose ◽  
Genome Sequences ◽  
Content Type

Draft genomes of two strains of Escherichia coli, FP2 and FP3, isolated from the feces of the Canada goose (Branta canadensis), were sequenced. Genome sizes were 5.26 Mb with a predicted G+C content of 50.54% (FP2) and 5.07 Mb with a predicted G+C content of 50.41% (FP3).

scholarly journals Cytotoxic and Apoptotic Effects of Recombinant Subtilase Cytotoxin Variants of Shiga Toxin-Producing Escherichia coli

2015 ◽  
Vol 83 (6) ◽  
pp. 2338-2349 ◽  
Author(s):  
J. Funk ◽  
N. Biber ◽  
M. Schneider ◽  
E. Hauser ◽  
S. Enzenmüller ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Vero Cells ◽  
Toxic Activity ◽  
Content Type ◽  
B Subunits ◽  
Induced Apoptosis ◽  
The Individual ◽  
Apoptotic Effects ◽  
Subtilase Cytotoxin

In this study, the cytotoxicity of the recently described subtilase variant SubAB2-2of Shiga toxin-producingEscherichia coliwas determined and compared to the plasmid-encoded SubAB1and the chromosome-encoded SubAB2-1variant. The genes for the respective enzymatic active (A) subunits and binding (B) subunits of the subtilase toxins were amplified and cloned. The recombinant toxin subunits were expressed and purified. Their cytotoxicity on Vero cells was measured for the single A and B subunits, as well as for mixtures of both, to analyze whether hybrids with toxic activity can be identified. The results demonstrated that all three SubAB variants are toxic for Vero cells. However, the values for the 50% cytotoxic dose (CD50) differ for the individual variants. Highest cytotoxicity was shown for SubAB1. Moreover, hybrids of subunits from different subtilase toxins can be obtained which cause substantial cytotoxicity to Vero cells after mixing the A and B subunits prior to application to the cells, which is characteristic for binary toxins. Furthermore, higher concentrations of the enzymatic subunit SubA1exhibited cytotoxic effects in the absence of the respective B1subunit. A more detailed investigation in the human HeLa cell line revealed that SubA1alone induced apoptosis, while the B1subunit alone did not induce cell death.

scholarly journals Draft Genome Sequences of Colistin-Resistant MCR-1-Producing Escherichia coli ST1850 and ST74 Strains Isolated from Commercial Chicken Meat

2017 ◽  
Vol 5 (20) ◽  
Author(s):  
Daniel F. Monte ◽  
Miriam R. Fernandes ◽  
Louise Cerdeira ◽  
Tiago A. de Souza ◽  
Andressa Mem ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Chicken Meat ◽  
Sequence Type ◽  
Genome Sequences ◽  
Content Type ◽  
Commercial Chicken

ABSTRACT We present here the draft genome sequences of two colistin-resistant mcr-1-carrying Escherichia coli strains belonging to sequence type 74 (ST74) and ST1850, isolated from commercial chicken meat in Brazil. Assembly of this draft genome resulted in 5,022,083 and 4,950,681 bp, respectively, revealing the presence of the IncX4 plasmid-mediated mcr-1 gene responsible for resistance to colistin.

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