Different secretory immunoglobulin A antibody responses to cholera vaccination in Swedish and Pakistani women

1980 ◽  
Vol 30 (2) ◽  
pp. 427-430
Author(s):  
A M Svennerholm ◽  
L A Hanson ◽  
J Holmgren ◽  
B S Lindblad ◽  
B Nilsson ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Secretory Immunoglobulin A ◽  
Cholera Vaccine ◽  
Intestinal Immunity ◽  
Significant Difference ◽  
Pakistani Women ◽  
Cholera Vaccination ◽  
Secretory Immunoglobulin

The capacity of subcutaneous cholera vaccination to induce an antibody response in milk and saliva was studied in lactating Swedish and Pakistani women, since secretory immunoglobulin A (SIgA) antibody responses in these secretions may reflect intestinal immunity. Before immunization, most of the Pakistani women had significant titers of specific SIgA antibodies against Vibrio cholerae lipopolysaccharide in milk, whereas only a few of the Swedish women had measurable, low titers. In the Pakistani women a single subcutaneous injection of cholera vaccine gave rise to a significant SIgA titer rise in 70% of the milk and 45% of the saliva samples. The Swedish women, on the other hand, did not respond with a significant antibody response of any immunoglobulin class in milk or saliva, either after a single or after a booster dose 14 days later. In serum, however, the vaccination induced significant titer rises, mainly of IgG antibodies, also in the Swedish women, but these rises were of lower magnitude than those in the Pakistani group. The results suggest a significant difference in the capacity of parenterally administered cholera vaccine to stimulate SIgA antibody formation in naturally primed and nonprimed individuals.

scholarly journals The immune landscape of IgA induction in the gut

2021 ◽  
Author(s):  
Claudia Seikrit ◽  
Oliver Pabst
Keyword(s):  
Protective Immunity ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Dark Side ◽  
Secretory Immunoglobulin A ◽  
Antibody Isotype ◽  
Mucosal Antibody ◽  
Key Concepts ◽  
Basic Understanding ◽  
Secretory Immunoglobulin

AbstractAntibodies are key elements of protective immunity. In the mucosal immune system in particular, secretory immunoglobulin A (SIgA), the most abundantly produced antibody isotype, protects against infections, shields the mucosal surface from toxins and environmental factors, and regulates immune homeostasis and a peaceful coexistence with our microbiota. However, the dark side of IgA biology promotes the formation of immune complexes and provokes pathologies, e.g., IgA nephropathy (IgAN). The precise mechanisms of how IgA responses become deregulated and pathogenic in IgAN remain unresolved. Yet, as the field of microbiota research moved into the limelight, our basic understanding of IgA biology has been taking a leap forward. Here, we discuss the structure of IgA, the anatomical and cellular foundation of mucosal antibody responses, and current concepts of how we envision the interaction of SIgA and the microbiota. We center on key concepts in the field while taking account of both historic findings and exciting new observations to provide a comprehensive groundwork for the understanding of IgA biology from the perspective of a mucosal immunologist.

scholarly journals Fecal Antibodies to Cryptosporidium parvum in Healthy Volunteers

2000 ◽  
Vol 68 (9) ◽  
pp. 5068-5074 ◽  
Author(s):  
Sara M. Dann ◽  
Pablo C. Okhuysen ◽  
Bassam M. Salameh ◽  
Herbert L. DuPont ◽  
Cynthia L. Chappell
Keyword(s):  
Antibody Response ◽  
Healthy Volunteers ◽  
Cryptosporidium Parvum ◽  
Immunoglobulin A ◽  
Reactivity Index ◽  
Secretory Immunoglobulin A ◽  
Low Doses ◽  
Previous Exposure ◽  
Oocyst Excretion ◽  
Secretory Immunoglobulin

ABSTRACT This study examined the intestinal antibody response in 26 healthy volunteers challenged with Cryptosporidium parvum oocysts. Fecal extracts were assayed for total secretory immunoglobulin A (IgA) and C. parvum-specific IgA reactivity. Specific IgA reactivity was standardized to IgA concentration and expressed as a reactivity index (RI). Anti-C. parvum fecal IgA (fIgA) increased significantly in 17 of 26 (65.4%) following oocyst ingestion. Of those with detectable responses, 59, 76.5, and 94.1% were positive by days 7, 14, and 30, respectively. Volunteers receiving high challenge doses (>1,000 and 300 to 500 oocysts) had higher RIs (RI = 5.57 [P = 0.027] and RI = 1.68 [P = 0.039], respectively) than those ingesting low doses (30 to 100 oocysts; RI = 0.146). Subjects shedding oocysts and experiencing a diarrheal illness had the highest fIgA reactivity. When evaluated separately, oocyst excretion was associated with an increased fIgA response compared to nonshedders (RI = 1.679 versus 0.024, respectively; P = 0.003). However, in subjects experiencing diarrhea with or without oocyst shedding, a trend toward a higher RI (P = 0.065) was seen. Extracts positive for fecal IgA were further examined for IgA subclass. The majority of stools contained both IgA1 and IgA2, and the relative proportions did not change following challenge. Also, no C. parvum-specific IgM or IgG was detected in fecal extracts. Thus, fecal IgA to C. parvum antigens was highly associated with infection in subjects who had no evidence of previous exposure and may provide a useful tool in detecting recent infections.

scholarly journals SALIVARY SECRETORY IMMUNOGLOBULIN A LEVEL OF CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA IN MAINTENANCE PHASE

2018 ◽  
Vol 11 (3) ◽  
pp. 50 ◽  
Author(s):  
Danar Pradipta Rani ◽  
Sarworini B Budiardjo ◽  
Margaretha Suharsini
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Immunoglobulin A ◽  
Maintenance Phase ◽  
Healthy Children ◽  
Secretory Immunoglobulin A ◽  
Laboratory Observation ◽  
Significant Difference ◽  
Salivary Secretory Immunoglobulin A ◽  
Secretory Immunoglobulin

 Objective: The objective of this research is to investigate the differences in salivary secretory immunoglobulin A (sIgA) levels between children with gingivitis who have acute lymphoblastic leukemia (ALL) and are in the maintenance phase of chemotherapy and the levels in healthy children with gingivitis.Methods: This study used cross-sectional and laboratory observation methods with 19 ALL children and 19 healthy children who all had mild gingivitis. Two mL of saliva were collected and stored at −200°C. Then, salivary sIgA levels were measured using the ELISA method.Results: The results indicate an insignificant difference (p>0.05) in salivary sIgA levels between the ALL children in the maintenance phase with the level of 21.38 (7.23–107.26) and 37.26 (11.24–135.63) for the healthy children.Conclusion: There is no significant difference in salivary sIgA levels between the ALL children in the maintenance phase and the healthy children.

scholarly journals The sIgA and histatin-5 expressions in saliva of early childhood caries and caries-free children based on period times

2021 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Dharli Syafriza ◽  
Heriandi Sutadi ◽  
Ameta Primasari ◽  
Yahwardiyah Siregar
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Weak Correlation ◽  
Histatin 5 ◽  
Significant Difference ◽  
Secretory Immunoglobulin ◽  
Childhood Caries

To analyze the level of sIgA and Histatin-5 expression in Early Childhood Caries (ECC) and caries-free children. Saliva was collected from 31 subjects of ECC and 29 caries-free (ages 9-32 months). The level expression of sIgA and Histatin 5 was analyzed by enzyme-linked immunosorbent assay. The distribution and frequency of subjects consist of boys 47% and girls 53% with the average of ages 1-12 month 21% and 13-24 month 79%.sIgA and Histatin 5 expressions differ from the month periods tested (1, 3, and 9 months) but do not show any significant difference from each examination period (1 with three months; p0.05: 0.116), (1 with nine months; p 0.05: 0.766), and (3 to 9 months; p0.05: 0.398). The expression of sIgA and Histatin 5 of boys and girls were not significant (p0.05:0.550) and weak correlation (r = 0.079).The level expression of sIgA in the Caries-free higher than ECC (p0.05). Whereas the expression of Histatin 5 in ECC higher than Caries-free (p0.05). The sIgA correlating in ECC and caries-free is significant (p0.05) and no correlation (r =- 0,29). Whereas Histatin 5 in the ECC and caries-free were strong correlation = 0,84. Both the result of ECC and caries-free suggested that the antibodies have been involved in the caries pathogenesis. In the ECC cases that occurred to decrease sIgA levels and increased of Histatin 5 levels, both antibodies have expressed in Caries-free Children. Both sIgA and Histatin 5 involved in the pathogenesis caries infection in children.KEYWORDS: Antibodies; Early Childhood Caries; saliva; secretory immunoglobulin A; Histatin-5

scholarly journals Immune Responses in Ileostomy Fluid and Serum after Oral Cholera Vaccination of Patients Colectomized because of Ulcerative Colitis

1998 ◽  
Vol 66 (8) ◽  
pp. 3995-3999 ◽  
Author(s):  
Jan Kilhamn ◽  
Hans Brevinge ◽  
Ann-Mari Svennerholm ◽  
Marianne Jertborn
Keyword(s):  
Ulcerative Colitis ◽  
Immune Responses ◽  
Cholera Toxin ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Cholera Vaccine ◽  
Whole Cell ◽  
B Subunit ◽  
Iga Antibody ◽  
Cholera Vaccination

ABSTRACT The capacity of an oral inactivated B-subunit–whole-cell cholera vaccine to induce immune responses in patients colectomized due to ulcerative colitis was studied. Two doses of vaccine induced significant mucosal immunoglobulin A (IgA) antibody responses in ileostomy fluid against cholera toxin in 14 of 15 (93%) patients and against whole vibrios in 9 of 15 (60%) cases. The serological responses were lower (but not significantly) than those observed in healthy Swedish volunteers. Increased IgA antitoxin levels were found in ileostomy fluid as late as 2 years after vaccination.

Sign in / Sign up

Export Citation Format

Share Document