scholarly journals Complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection.

1981 ◽  
Vol 31 (2) ◽  
pp. 636-640 ◽  
Author(s):  
D B Snyder ◽  
A C Myrup ◽  
S K Dutta
Keyword(s):  
Virus Neutralization ◽  
Equine Herpesvirus ◽  
Serum Complement ◽  
Equine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Investigations on the presence of antibodies against equine herpesvirus-1 in blood serum of foals, prior to and after colostrum intake

2015 ◽  
Vol 69 (3-4) ◽  
pp. 195-204
Author(s):  
Sasa Laus ◽  
Ljubica Spasojevic-Kosic ◽  
Sava Lazic ◽  
Dragisa Trailovic
Keyword(s):  
Blood Serum ◽  
Virus Neutralization ◽  
Equine Herpesvirus ◽  
Blood Samples ◽  
Equine Herpesvirus 1 ◽  
Specific Antibodies ◽  
Microtiter Plates ◽  
Constant Dose ◽  
Herpesvirus 1

The titer of specific antibodies against equine herpesvirus-1 in blood serum was tested in two groups of mares and their foals. The first group consisted of 12 mares, Standardbred and Serbian Trotter breed, who were vaccinated against equine herpesvirus-1 and 4 in the 5th, 7th and 9th month of pregnancy. On the contrary, 12 mares from the second group, of Lipizzaner breed, were not vaccinated. The mares? blood samples for antibodies titer investigation were taken 30, 15 and 7 days before the expected partus, then immediately after the partus, while their foals? blood samples were taken immediately after foaling, then just before colostrum intake, and finally 1, 2, 3 and 7 days later. The titer of antibodies against equine herpesvirus-1 was tested by the method of virus - neutralization, on microtiter plates with constant dose of the virus and serial double dilutions of the serum. In unvaccinated mares, titer of antibodies against equine herpesvirus-1 was either low or not present, but on the contrary, in the vaccinated ones the antibodies titer ranged from 1:32 to 1:256. In the foals originating from both vaccinated and unvaccinated there were not found specific antibodies in the serum before colostrum intake. After the colostrum intake, the values of specific antibodies against equine herpesvirus-1 significantly increased in the foals originating from the vaccinated mares, and ranged from 1:8 to 1:32.

scholarly journals Immunological Correlates of Vaccination and Infection for Equine Herpesvirus 1

2011 ◽  
Vol 19 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Laura B. Goodman ◽  
Christine Wimer ◽  
Edward J. Dubovi ◽  
Carvel Gold ◽  
Bettina Wagner
Keyword(s):  
Experimental Studies ◽  
Virus Neutralization ◽  
Strongly Correlated ◽  
Equine Herpesvirus ◽  
Immunological Parameters ◽  
Live Virus ◽  
Equine Herpesvirus 1 ◽  
Ifn Γ ◽  
Herpesvirus 1 ◽  
High Throughput Manner

ABSTRACTEquine herpesvirus 1 (EHV-1) induces a variety of disease manifestations, including respiratory disease, abortions, and myeloencephalopathy. Several vaccines are commercially available but could not previously be distinguished by serologic testing from infection with EHV-1 (or the closely related EHV-4). Currently available vaccines are not reliably protective against the severe manifestations of the disease, including fatal myeloencephalopathy. We determined immunological parameters that can differentiate vaccinated from previously infected animals by comparing humoral and cellular EHV-1-specific responses in clinically healthy horses 10 months after vaccination. Forty-seven horses with known histories of vaccination and infection were studied, including a group of horses that survived a severe neurological outbreak 5 years prior to vaccination. Results of serum virus neutralization (SN), serum IgG isotyping, and cytokine profiling of lymphocyte subsets were compared. IgG4/7 levels strongly correlated with virus neutralization (P< 0.0001). IgG1/3 and SN values distinguished vaccinated/outbreak-exposed (vacc/outbreak) horses from vaccinated horses (P< 0.05). EHV-1-specific gamma interferon (IFN-γ)-producing CD4+(but not CD8+) T-cell numbers were also increased in vacc/outbreak horses, which distinguished them from vaccinated horses (P< 0.01). IFN-α secretion was similar between all groups and independent of previous exposure or vaccination. Our data suggest that IgG isotype responses to EHV-1 are more diverse under field conditions than is revealed by experimental studies and that the current modified-live virus (MLV) vaccine induces a more restricted IgG isotype response than does natural exposure to EHV-1. Since these parameters can be assessed in a high-throughput manner, they may prove useful in screening future vaccine candidates and assessing levels of protection.

The highly O-glycosylated glycoprotein gp2 of equine herpesvirus 1 is encoded by gene 71.

1996 ◽  
Vol 70 (11) ◽  
pp. 8195-8198 ◽  
Author(s):  
J E Wellington ◽  
G P Allen ◽  
A A Gooley ◽  
D N Love ◽  
N H Packer ◽  
...  
Keyword(s):  
Equine Herpesvirus ◽  
Equine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 425
Author(s):  
Hyung-Woo Kang ◽  
Eun-Yong Lee ◽  
Kyoung-Ki Lee ◽  
Mi-Kyeong Ko ◽  
Ji-Young Park ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
South Korea ◽  
Molecular Level ◽  
Economic Losses ◽  
Equine Herpesvirus ◽  
Equine Herpesvirus 1 ◽  
Mlst Analysis ◽  
Herpesvirus 1 ◽  
First Time ◽  
Group 1

Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7–100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701–818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.

Infection with Equine Herpesvirus 1 (EHV-1) Strain HVS25A in Pregnant Mice

1999 ◽  
Vol 120 (1) ◽  
pp. 15-27 ◽  
Author(s):  
C. Walker ◽  
V.M. Perotti ◽  
D.N. Love ◽  
J.M. Whalley
Keyword(s):  
Equine Herpesvirus ◽  
Equine Herpesvirus 1 ◽  
Pregnant Mice ◽  
Herpesvirus 1

Argentine strain of equine herpesvirus 1 isolated from an aborted foetus shows low virulence in mouse respiratory and abortion models

2004 ◽  
Vol 103 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
C.M. Galosi ◽  
C.G. Barbeito ◽  
M.V. Vila Roza ◽  
V. Cid de la Paz ◽  
M.A. Ayala ◽  
...  
Keyword(s):  
Equine Herpesvirus ◽  
Equine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Transcriptomic Profiling of Equine and Viral Genes in Peripheral Blood Mononuclear Cells in Horses during Equine Herpesvirus 1 Infection

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 43
Author(s):  
Lila M. Zarski ◽  
Patty Sue D. Weber ◽  
Yao Lee ◽  
Gisela Soboll Hussey
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Differentially Expressed ◽  
Equine Herpesvirus ◽  
Peripheral Blood Mononuclear ◽  
Equine Herpesvirus 1 ◽  
Blood Mononuclear Cells ◽  
Herpesvirus 1

Equine herpesvirus 1 (EHV-1) affects horses worldwide and causes respiratory disease, abortions, and equine herpesvirus myeloencephalopathy (EHM). Following infection, a cell-associated viremia is established in the peripheral blood mononuclear cells (PBMCs). This viremia is essential for transport of EHV-1 to secondary infection sites where subsequent immunopathology results in diseases such as abortion or EHM. Because of the central role of PBMCs in EHV-1 pathogenesis, our goal was to establish a gene expression analysis of host and equine herpesvirus genes during EHV-1 viremia using RNA sequencing. When comparing transcriptomes of PBMCs during peak viremia to those prior to EHV-1 infection, we found 51 differentially expressed equine genes (48 upregulated and 3 downregulated). After gene ontology analysis, processes such as the interferon defense response, response to chemokines, the complement protein activation cascade, cell adhesion, and coagulation were overrepresented during viremia. Additionally, transcripts for EHV-1, EHV-2, and EHV-5 were identified in pre- and post-EHV-1-infection samples. Looking at micro RNAs (miRNAs), 278 known equine miRNAs and 855 potentially novel equine miRNAs were identified in addition to 57 and 41 potentially novel miRNAs that mapped to the EHV-2 and EHV-5 genomes, respectively. Of those, 1 EHV-5 and 4 equine miRNAs were differentially expressed in PBMCs during viremia. In conclusion, this work expands our current knowledge about the role of PBMCs during EHV-1 viremia and will inform the focus on future experiments to identify host and viral factors that contribute to clinical EHM.

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