scholarly journals Cell-mediated immunity to Chlamydia pneumoniae measured as lymphocyte blast transformation in vitro.

1993 ◽  
Vol 61 (5) ◽  
pp. 2196-2199 ◽  
Author(s):  
H M Surcel ◽  
H Syrjälä ◽  
M Leinonen ◽  
P Saikku ◽  
E Herva
Keyword(s):  
Chlamydia Pneumoniae ◽  
Cell Mediated Immunity ◽  
Blast Transformation ◽  
Lymphocyte Blast Transformation

scholarly journals Immunomodulatory Activity and Phytochemical Profile of Infusions from Cleavers Herb

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3721 ◽  
Author(s):  
Tetiana Ilina ◽  
Weronika Skowrońska ◽  
Natalia Kashpur ◽  
Sebastian Granica ◽  
Agnieszka Bazylko ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Herbal Remedy ◽  
Folk Medicine ◽  
Immunomodulatory Activity ◽  
Blast Transformation ◽  
Immunostimulatory Activity ◽  
Traditional Use ◽  
Aerial Parts ◽  
Lymphocyte Blast Transformation

Extracts from aerial parts of G. aparine (cleavers) constitute a herbal remedy with monography in British Herbal Pharmacopeia. On the European market, there are several drugs and food supplements consisting of Galium extracts. In folk medicine, cleavers was used topically in Europe, Asia, and the Americas to treat skin diseases. In several remedies, cleavers is also listed as an immunomodulatory active herb influencing the defense response of the human body. The aim of this study was to investigate the immunostimulatory activity and antioxidant potential in vitro of a raw infusion of cleavers and bioactive fractions. The functional activity of lymphocytes in the reaction of the lymphocyte blast transformation (RLBT) method was used for immunomodulatory activity assays and direct scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydrogen peroxide (H2O2) was chosen for the examination of antioxidant activity. It was shown that both the raw extract and fractions show significant immunostimulatory and scavenging activities. The obtained data partially justify the traditional use of cleavers as topical remedy for skin infections and for wounds.

In Vitro Development of Macrophages from PHA-stimulated Blood Cultures in Hodgkin's Disease and in Chronic Lymphocytic Leukaemia.

1976 ◽  
Vol 62 (1) ◽  
pp. 19-24
Author(s):  
Roberto Navone ◽  
Mauro Tosto ◽  
Ester Poggio
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Hodgkin's Disease ◽  
Lymphocytic Leukaemia ◽  
Hodgkin’S Disease ◽  
Normal Subjects ◽  
Blood Cultures ◽  
Blast Transformation ◽  
Lymphocyte Blast Transformation ◽  
Vitro Development

Blood was cultured from 17 normal subjects, 28 cases of untreated chronic lymphocytic leukaemia (CLL) and 41 cases of Hodgkin's disease. Macrophages were not observed in PHA-stimulated cultures of normal subjects, of CLL or of 18 cases of Hodgkin's disease. The latter had an almost normal rate of lymphocyte blast transformation (70,2 ± 7,2%). On the other hand, macrophages were numerous in PHA-stimulated cultures of 23 cases of Hodgkin's disease with a low blast transformation (28,4 ± 13,7%). In Hodgkin's disease the development of macrophages in PHA cultures may be related to the lower blast transformation and cytotoxic activity of lymphocytes. In CLL the dilution of lymphocytes and monocytes by leukaemic B cells may account both for PHA unresponsiveness and low yield of macrophages in culture.

scholarly journals Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L.

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 541 ◽  
Author(s):  
Tetiana Ilina ◽  
Natalia Kashpur ◽  
Sebastian Granica ◽  
Agnieszka Bazylko ◽  
Igor Shinkovenko ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Hydroxycinnamic Acid ◽  
Immunomodulatory Activity ◽  
Blast Transformation ◽  
Galium Aparine ◽  
Hydroxycinnamic Acid Derivatives ◽  
Ethanolic Extracts ◽  
Lymphocyte Blast Transformation ◽  
Folk Remedies

Galium aparine L., family Rubiaceae, is a widely spread species in the Galium genus. The herb of G. aparine is part of folk remedies and dietary supplements. In this study, we analyzed the chemical composition and immunomodulatory activities of G. aparine herb ethanolic extracts obtained from the plant material by maceration with 20%, 60% or 96% ethanol. The contents of hydroxycinnamic acid derivatives, flavonoids and polyphenols were determined spectrophotometrically, with extractives and polysaccharides quantified gravimetrically. The qualitative composition was studied using UHPLC-DAD-MS/MS analysis; isolation not previously described in G. aparine quercetin rhamnoglucoside was carried out through column chromatography, and the immunomodulatory activity of extracts was determined in the reaction of lymphocyte blast transformation. Major constitutes of extracts were iridoids, i.e., monotropein, 10-desacetylasperulosidic acid and asperulosidic acid; p-hydroxybenzoic acid; hydroxycinnamic acid derivatives, i.e., 3-O-caffeoylquinic, 5-O-caffeoylquinic, 3,4-O-dicaffeoylquinic, 3,5-O-dicaffeoylquinic, 4,5-O-dicaffeoylquinic acids and caffeic acid derivatives; flavonoids, i.e., rutin, quercetin 3-O-rhamnoglucoside-7-O-glucoside, and isorhamnetin 3-O-glucorhamnoside. Significantly, quercetin 3-O-rhamnoglucoside-7-O-glucoside was first isolated and identified in Galium species so far investigated. All G. aparine herb ethanolic extracts stimulate the transformational activity of immunocompetent blood cells, with 96% ethanolic extract being the most active. The data obtained necessitate further research into the mechanisms of immunomodulatory activity of extracts from G. aparine herb.

scholarly journals Taraxacum Officinale Alcoholic Extract Augments the Cell-Mediated Immune Response in Anti-Anthrax Vaccinated Sheep

2021 ◽  
Vol 78 (1) ◽  
pp. 109
Author(s):  
Petru POJAR ◽  
Carmen Dana ȘANDRU ◽  
Elisabeta PASTOR ◽  
Constantin CERBU ◽  
Silvana POPESCU ◽  
...  
Keyword(s):  
Taraxacum Officinale ◽  
Cell Mediated Immunity ◽  
Blast Transformation ◽  
Therapeutic Effects ◽  
Alcoholic Extract ◽  
Cell Mediated Immune Response ◽  
Blastogenic Response ◽  
Positive Effect ◽  
Over Time

Phytotherapy used various preparations in both human and veterinary medicine over time, due to their increased bioavailability and lower costs.  Taraxacum officinale of Compositae family, well-known for its therapeutic effects, abundantly grows on Romanian pastures. The research aimed to investigate its alcoholic extract’s effects on cell-mediated immunity in anti-anthrax vaccinated, grazing sheep. Local Turcana ewes kept on pasture from spring to fall were sc vaccinated with R1190 attenuated anthrax strain, and blood was sampled before and 14 days after the vaccination. In vitro cell-mediated immunity was monitored employing phagocytosis and blast transformation tests in the presence of T. officinale alcoholic extract. The extract increased phagocytosis two weeks after the vaccination (p<0.001), while the blastogenic response also showed significant variations (p<0.05) over time.  There was a positive effect of T. officinale extract on the cellular immunity, suggesting its stress alleviating effects in anti-anthrax vaccinated sheep.

scholarly journals CD69 Expression on CD4+ T Lymphocytes after In Vitro Stimulation with Tuberculin Is an Indicator of Immune Sensitization against Mycobacterium tuberculosis Antigens

2005 ◽  
Vol 12 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Bojana Avgustin ◽  
Vladimir Kotnik ◽  
Mojca Škoberne ◽  
Tadej Malovrh ◽  
Aleksandra Skralovnik-Stern ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Lymphocytes ◽  
Antigen Expression ◽  
Blast Transformation ◽  
Healthy Individuals ◽  
Purified Protein Derivative ◽  
Lymphocyte Blast Transformation ◽  
Cd69 Expression ◽  
Group B

ABSTRACT The expression of the CD69 antigen on CD4 T lymphocytes after in vitro stimulation with purified protein derivative (2 tuberculin units) was used to evaluate the tuberculin reactivities of 52 individuals from four experimental groups: Mycobacterium bovis BCG-vaccinated healthy individuals with a negative tuberculin skin test (TST) result (group A), BCG-vaccinated healthy individuals with a positive TST result (group B), patients with active tuberculosis (TB) before treatment (group C), and individuals with clinically inactive TB who had previously completed a prescribed course of chemotherapy (group D). The expression of CD69 on CD4 T lymphocytes was significantly higher in patients with active TB (16.2% ± 7.3%), individuals with clinically inactive TB (10.5% ± 7.4%), and healthy individuals with a positive TST result (15.5% ± 7.2%) than in healthy individuals with a negative TST result (3.8% ± 4.3%) (P < 0.005). We confirmed the correlation between CD69 antigen expression on T lymphocytes after stimulation with tuberculin and the TST induration diameter (Spearman rho = 0.783; P < 0.001), an assay for gamma interferon (the Quantiferon-TB assay; Spearman rho = 0.613; P < 0.001), and the lymphocyte BLAST transformation test (Spearman rho = 0.537; P < 0.001). Our results demonstrate the usefulness of the determination of CD69 on CD4 T lymphocytes after in vitro stimulation with tuberculin as a rapid indicator of immune sensitization against Mycobacterium tuberculosis.

scholarly journals Cell-mediated immunity in visceral obesity

2016 ◽  
pp. 12-19
Author(s):  
N. Asfandiyarova ◽  
N. Zhuravleva ◽  
A. Skopin ◽  
A. Borozdin ◽  
T. Trunina ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immune System ◽  
Visceral Obesity ◽  
Autoimmune Response ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Blast Transformation ◽  
Type 2 Dm ◽  
Lymphocyte Blast Transformation

Visceral obesity is associated with the development of several diseases, such as osteoarthritis (OA), cardiovascular disease (CVD), type 2 diabetes mellitus (DM), and breast cancer (BC). Recent years have discussed the value of the immune system in the pathogenesis of the disease, but its role remains unclear. The aim of the study was to investigate the cellular immune responses in diseases comorbid with visceral obesity (OA, CVD, type 2 DM, BC). The study included 36 patients with OA, 61 – CVD, 60 - type 2 DM, 98 - breast cancer in postmenopausal women, 30 patients with benign breast tumors. The control group involved 21 healthy people. Our study includes anthropometric, clinical, biochemical, immunological (the reaction of lymphocyte blast transformation to PHA, insulin, antigens cartilage, IGF-1) and morphological methods of investigation. Patients with obesity associated diseases have reduction of proliferative activity in response to PHA and increased frequency of autoimmune reactions to insulin suppressed by prostaglandin-synthesizing cells and/or cells with histamine receptors (144/239 vs. 2/21, p <0,001). This type of cell-mediated immunity is correlated with the anthropometric data (BMI, waist circumference, WC/height) and disorders of carbohydrate metabolism. Conclusions. Despite the differences of diseases associated with visceral obesity, the fact united them is the presence of metabolic disorders, the development of an autoimmune response to insulin, suppressed by prostaglandin synthesizing cells and/or cells with receptors to histamine (more than 60%), which suggests the involvement of the immune system in the genesis of these disorders.

Immediate Loss of Cell-Mediated Immunity to Murine Cytomegalovirus upon Treatment with Immunosuppresive Agents

1980 ◽  
Vol 30 (3) ◽  
pp. 700-708
Author(s):  
Donald M. Mattsson ◽  
Richard J. Howard ◽  
Henry H. Balfour
Keyword(s):  
Cytomegalovirus Infection ◽  
Proliferative Response ◽  
Murine Cytomegalovirus ◽  
Cell Mediated Immunity ◽  
Blast Transformation ◽  
Specific Cell ◽  
Antilymphocyte Globulin ◽  
Prednisolone Treatment

Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted through a 2-week course of immunosuppression. Circulating specific antibodies were depressed by multiple injections of antilymphocyte globulin alone or with prednisolone, but not by prednisolone alone. Mitogen-induced blast transformation was immediately depressed by immunosuppression with both agents. Although the response to lipopolysaccharide returned briefly, it declined with continuing treatment. Cytomegalovirus infection augmented the depressive effect of immunosuppression on the lipopolysaccharide proliferative response. Prednisolone treatment of infected animals did not affect the concanavalin A response, and lipopolysaccharide stimulation decreased more slowly and to a lesser extent than it did in mice treated with antilymphocyte globulin or both agents. Loss of specific cell-mediated immunity and simultaneous depression of humoral immunity indicated that immunosuppression immediately created an inability to respond to an active cytomegalovirus infection.

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