Site-Directed Mutagenesis Identifies a Molecular Switch Involved in Copper Sensing by the Histidine Kinase CinS in Pseudomonas putida KT2440
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ABSTRACT In the presence of copper, Pseudomonas putida activates transcription of cinAQ via the two-component system CinS-CinR. The CinS-CinR TCS was responsive to 0.5 μM copper and was specifically activated only by copper and silver. Modeling studies of CinS identified a potential copper binding site containing H37 and H147. CinS mutants with H37R and H147R mutations had an almost 10-fold reduced copper-dependent induction of cinAQ compared to the wild type.
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2005 ◽
Vol 187
(3)
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pp. 1105-1113
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2000 ◽
Vol 182
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pp. 937-943
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2018 ◽
2019 ◽
Vol 519
(1)
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pp. 198-203
2005 ◽
Vol 187
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pp. 7317-7324
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