Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System
2000 ◽
Vol 182
(3)
◽
pp. 842-847
◽
Keyword(s):
E Coli
◽
ABSTRACT We describe a simple system for reversible, stable integration of plasmid-borne genes into the Escherichia coli chromosome. Most ordinary E. coli strains and a variety of pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, a lambda phage, is the only specialized vector required. The resultant strains have a single copy of the plasmid fragment inserted stably at the lambda attachment site on the chromosome, with nearly the entire lambda genome deleted.
Keyword(s):
Keyword(s):
1990 ◽
Vol 265
(4)
◽
pp. 2352-2357
Keyword(s):
Keyword(s):
2015 ◽
Vol 84
◽
pp. 205-219
◽
Keyword(s):