scholarly journals Relationship of Hydrogen Peroxide Production by Mycoplasma pulmonis to Virulence for Catalase-deficient Mice

1969 ◽  
Vol 98 (3) ◽  
pp. 1036-1040 ◽  
Author(s):  
Patricia C. Brennan ◽  
Robert N. Feinstein
Author(s):  
Keiichi Moriguchi ◽  
Kei-Ichi Hirai

The ability of hydrogen peroxide (H2O2) production was cytochemically compared in eosinophils (EP) between specific pathogen free (SPF) and spontaneously Mycoplasma pulmonis-infected male rats (Wistar strain).Peritoneal cells including EP (PEP) were harvested with a cold Hanks' balanced salt solution containing 0.1% glucose (HBSSG). Simultaneously, blood granulocytes with eosinophils (BEP) were isolated from the same animals by a Ficoll-Hypaque method. Cells were incubated with latex particles in HBSSG for 60-90 min. Thereafter, cells were washed and transferred into a cerium (Ce) medium consisting of 0.1 M tris - maleate buffer, pH 7.5, 1mM CeCl3, 10 mM sodium azide, 0.1. glucose and 0.25 M sucrose. The incubation was carried out for 20 min at 37°C with occasional stirring. Some cells were incubated for 60 min at 37 °C in a DAB medium containing 0.1% 3.3’-diaminobenzidine 4HCl with particles in 0.1 M phosphate buffer, pH 7.4. All cells were then fixed with glutaraldehyde and osmium tetroxide before processing for electron microscopy, x-ray microanalysis of the subcellular electron-dense reaction deposits was performed under a Tracor-Northern EDX attached to a JEM-1200EX STEM system.


2017 ◽  
Vol 12 (10) ◽  
pp. 1111-1119 ◽  
Author(s):  
Kojiro Fuku ◽  
Yuta Miyase ◽  
Yugo Miseki ◽  
Takashi Funaki ◽  
Takahiro Gunji ◽  
...  

Author(s):  
Yu Guo ◽  
Qing Xu ◽  
Shuai Yang ◽  
Zheng Jiang ◽  
Chengbing Yu ◽  
...  

2009 ◽  
Vol 47 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Carla A. Di Maria ◽  
Marie A. Bogoyevitch ◽  
Douglas J. McKitrick ◽  
Leonard F. Arnolda ◽  
Livia C. Hool ◽  
...  

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