scholarly journals Detection of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Phage Types DT102, DT104, and U302 by Multiplex PCR

2006 ◽  
Vol 44 (7) ◽  
pp. 2354-2358 ◽  
Author(s):  
C.-H. Chiu ◽  
L.-H. Su ◽  
C.-H. Chu ◽  
M.-H. Wang ◽  
C.-M. Yeh ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Multidrug Resistant ◽  
Phage Types ◽  
Serovar Typhimurium

scholarly journals Characterization of Salmonella enterica Serovar Typhimurium from Marine Environments in Coastal Waters of Galicia (Spain)

2004 ◽  
Vol 70 (7) ◽  
pp. 4030-4034 ◽  
Author(s):  
Jaime Martinez-Urtaza ◽  
Ernesto Liebana ◽  
Lourdes Garcia-Migura ◽  
Pelayo Perez-Piñeiro ◽  
Montserrat Saco
Keyword(s):  
Coastal Waters ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Marine Environments ◽  
Pfge Analysis ◽  
Phage Types ◽  
Plasmid Analysis ◽  
Routine Surveillance ◽  
Serovar Typhimurium ◽  
Plasmid Profiling

ABSTRACT Twenty-three Salmonella enterica serovar Typhimurium isolates from marine environments were characterized by phage typing, pulsed-field gel electrophoresis (PFGE) analysis, plasmid analysis, and antibiotic resistance, and the distribution of the different types in the coastal waters were subsequently analyzed. Five phage types were identified among the isolates (PT41, PT135, PT99, DT104, and DT193). PT135 isolates were exclusively detected during the winter months from 1998 to 2000, whereas DT104 and PT41 isolates were detected exclusively in the summer months from 2000 to 2002. XbaI PFGE analysis revealed 9 PFGE types, and plasmid profiling identified 8 plasmid types (with 1 to 6 plasmids) among the isolates. Only three isolates presented multidrug resistance to antibiotics. Two DT104 isolates were resistant to 8 and 7 antibiotics (profiles ACCeFNaSSuT and ACeFNeSSuT), whereas a PT193 isolate presented resistance to 6 antibiotics (profile ACFSSu). In addition, four PT41 isolates were resistant to a single antibiotic. The detection of multidrug-resistant phage types DT104 and DT193 in shellfish emphasizes the importance of monitoring the presence of Salmonella in routine surveillance of live bivalve molluscs.

scholarly journals Isolation and Characterization of Salmonella Enterica Serovar Typhimurium Circulating Among Healthy Chickens of Bangladesh

2016 ◽  
Vol 4 (7) ◽  
pp. 519
Author(s):  
Md. Shafiullah Parvej ◽  
Marzia Rahman ◽  
Md. Forhad Uddin ◽  
KHM Nazmul Hussain Nazir ◽  
Md. Sayduzzaman Jowel ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Multidrug Resistant ◽  
Cloacal Swab ◽  
Specific Gene ◽  
Isolation And Characterization ◽  
Serovar Typhimurium ◽  
Salmonella Serovars ◽  
Layer Chickens ◽  
Apparently Healthy

Salmonella is considered as a global problem ranking first among food borne diseases. All motile Salmonella of poultry origin are zoonotic and readily transmit to human via meat and eggs but reports on non - typhoidal Salmonella serovars circulating in layer chickens is very limited in South-East Asian countries including Bangladesh. Salmonella serovars isolated from apparently healthy chickens were characterized in the present study. Of 170 samples (cloacal swab 150 and feed 20) collected from commercial layer farms, motile Salmonella was isolated 4% (6/150) and 50% (10/20) respectively by cultural, biochemical, motility test and by detection of hisJ gene. About 5% (8/170) samples possessed serovar-specific gene fimA, suggesting that isolates were Salmonella enterica serovar Typhimurium. Antimicrobial susceptibility testing demonstrated that the isolated serovars were multidrug resistant. Therefore apparently healthy layer chickens harbour and transmit S. Typhimurium to the environment, although little is alarming since it has zoonotic significance and the isolates were resistant to commonly used first line of antibiotic in Salmonella infection.

scholarly journals Antibiotic Resistance in Salmonella enterica Serovar Typhimurium Exposed to Microcin-Producing Escherichia coli

2001 ◽  
Vol 67 (8) ◽  
pp. 3763-3766 ◽  
Author(s):  
Steve A. Carlson ◽  
Timothy S. Frana ◽  
Ronald W. Griffith
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Pathogenic Bacteria ◽  
Defense Mechanism ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Multidrug Resistant ◽  
E Coli ◽  
Resistant Phenotype ◽  
Serovar Typhimurium

ABSTRACT Microcin 24 is an antimicrobial peptide secreted by uropathogenicEscherichia coli. Secretion of microcin 24 provides an antibacterial defense mechanism for E. coli. In a plasmid-based system using transformed Salmonella enterica, we found that resistance to microcin 24 could be seen in concert with a multiple-antibiotic resistance phenotype. This multidrug-resistant phenotype appeared when Salmonella was exposed to an E. coli strain expressing microcin 24. Therefore, it appears that multidrug-resistant Salmonellacan arise as a result of an insult from other pathogenic bacteria.

scholarly journals Genome Sequences of 13 Isolates of Salmonella enterica Serovar Typhimurium var. Copenhagen Obtained from Wild Pigeons in Canada

2018 ◽  
Vol 6 (20) ◽  
Author(s):  
Bridget Xie ◽  
Andrée Ann Dupras ◽  
Marc-Olivier Duceppe ◽  
Nooshin Fattahi-Ghazi ◽  
Lawrence Goodridge ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Virulence Plasmid ◽  
Avian Host ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Phage Types ◽  
Serovar Typhimurium

ABSTRACT Pigeon-adapted strains of Salmonella enterica serovar Typhimurium var. Copenhagen phage types 2 and 99 obtained from the provinces of Alberta, British Columbia, and Ontario, Canada, were analyzed using whole-genome sequencing. All isolates contained the Salmonella virulence plasmid despite the low pathogenicity of this lineage in their avian host.

scholarly journals Involvement of a Salmonella Genomic Island 1 Gene in the Rumen Protozoan-Mediated Enhancement of Invasion for Multiple-Antibiotic-Resistant Salmonella enterica Serovar Typhimurium

2006 ◽  
Vol 75 (2) ◽  
pp. 792-800 ◽  
Author(s):  
Steve A. Carlson ◽  
Vijay K. Sharma ◽  
Zoe P. McCuddin ◽  
Mark A. Rasmussen ◽  
Sharon K. Franklin
Keyword(s):  
Salmonella Enterica ◽  
Genomic Island ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Phage Type ◽  
Reporter System ◽  
Antibiotic Resistant ◽  
Phage Types ◽  
Food Borne ◽  
Serovar Typhimurium ◽  
Invasion Assays

ABSTRACT Multiple-antibiotic-resistant Salmonella enterica serotype Typhimurium is a food-borne pathogen that may be more virulent than related strains lacking the multiresistance phenotype. Salmonella enterica serotype Typhimurium phage type DT104 is the most prevalent of these multiresistant/hypervirulent strains. Multiresistance in DT104 is conferred by an integron structure, designated Salmonella genomic island 1 (SGI1), while we recently demonstrated DT104 hyperinvasion mediated by rumen protozoa (RPz) that are normal flora of cattle. Hyperinvasion was also observed in other Salmonella strains, i.e., other S. enterica serovar Typhimurium phage types and other S. enterica serovars, like S. enterica serovar Infantis, possessing SGI1, while DT104 strains lacking SGI1 were not hyperinvasive. Herein we attempted to identify SGI1 genes involved in the RPz-mediated hyperinvasion of Salmonella strains bearing SGI1. Transposon mutagenesis, coupled with a novel reporter system, revealed the involvement of an SGI1 gene previously designated SO13. Disruption of SO13 expression led to an abrogation of hyperinvasion as assessed by tissue culture invasion assays and by bovine challenge experiments. However, hyperinvasion was not observed in non-SGI1-bearing strains of Salmonella engineered to express SO13. That is, SO13 and another SGI1 gene(s) may coordinately upregulate invasion in DT104 exposed to RPz.

Sign in / Sign up

Export Citation Format

Share Document