scholarly journals A Cost-Effective Method for Identifying Enterobacterales with OXA-181

2019 ◽  
Vol 57 (11) ◽  
Author(s):  
Gisele Peirano ◽  
Yasufumi Matsumura ◽  
Diego Nobrega ◽  
Johann D. D. Pitout
Keyword(s):  
Indian Subcontinent ◽  
Cost Effective ◽  
Accurate Information ◽  
Surveillance Program ◽  
Molecular Surveillance ◽  
Specific Pcr ◽  
Cost Effective Method ◽  
Molecular Studies ◽  
Surveillance Programs ◽  
Positive Results

ABSTRACT OXA-181 is the second most common global OXA-48-like carbapenemase and is endemic in the Indian subcontinent. Molecular studies have shown that Enterobacterales with OXA-181 are often introduced into regions of nonendemicity. Distinguishing OXA-181 from other OXA-48-like enzymes often requires sequencing, which is rather expensive and time-consuming. A specific PCR (i.e., OXA181PCR) for the detection of blaOXA-181 was validated using a global collection (n = 315) of bacteria with well-characterized carbapenemases and showed 100% sensitivity and specificity (95% confidence interval [CI], 94.1 to 100 and 98.6 to 100, respectively) for detecting bacteria with OXA-181. The OXA181PCR subsequently gave positive results on 58/160 (36%) Enterobacterales with OXA-48-like carbapenemases from the 2015 INFORM surveillance program. The blaOXA-181-positive Enterobacterales were present in 9 countries spanning 5 continents, illustrating the global distribution of OXA-181. This methodology can easily be incorporated into molecular surveillance programs to provide accurate information about the prevalence of OXA-181. A loop-mediated isothermal amplification (LAMP)-OXA48 assay overall performed well for detecting OXA-48-like enzymes but showed poor specificity due to false-positive results with non-OXA carbapenemases.

PP95 Engagement Of Local Policymakers In HTA With Positive Results

2018 ◽  
Vol 34 (S1) ◽  
pp. 101-102
Author(s):  
Flavia Elias ◽  
Luciana Gallo ◽  
Ana Carolina Pereira ◽  
Erica Silva ◽  
Juliana Girardi ◽  
...  
Keyword(s):  
Primary Care ◽  
Health Care ◽  
Pulmonary Tuberculosis ◽  
Homeless People ◽  
Control Program ◽  
Professional Staff ◽  
Surveillance Program ◽  
Overview Of Systematic Reviews ◽  
Surveillance Programs ◽  
Positive Results

Introduction:São Paulo city in Brazil has implemented social and health care for homeless people with pulmonary tuberculosis since 2007. We conducted a health technology assessment (HTA) of the interventions provided based on a national theoretical model using 2015 data and an overview of systematic reviews. The HTA was requested by national policymakers. The results demonstrated that the interventions for pulmonary tuberculosis were satisfactory. The municipal secretariat implemented actions to improve the national treatment recommendations and adopted incentives to increase adherence to treatments. Our objective was to describe the feedback process for the Health Secretariat.Methods:The feedback was categorized as: (i) an executive abstract with key messages (i.e. ninety-seven percent of notified cases underwent sputum smears, nineteen percent were hospitalized, and fifty-nine percent were cured) reported to policymakers involved in the surveillance program; and (ii) three meetings were organized jointly by the research group and local policymakers.Results:In 2016 we conducted a meeting to present the results. Thirty-nine professionals involved in the primary care team working on the streets (thirty-five percent) and the Tuberculosis Surveillance and Control Program (five percent) were present. The main barriers presented by the professionals were issues of human resources (i.e. suboptimal professional staff and having two different social organizations responsible for health care). The main facilitators presented by professionals were: (i) using homeless-peers as healthcare workers; (ii) having a network linking the primary care and surveillance programs; and (iii) periodic training.Conclusions:In addition to the positive results, the HTA presented an opportunity to discuss the sustainability of incentives for adhering to treatments adopted by the policymakers, such as meal allowances and housing support, to improve social conditions among the homeless.

The importance of diagnosis of TORCH-infections before using assisted reproductive technologies programs

2018 ◽  
pp. 20-22
Author(s):  
V.V. Kaminskyy ◽  
◽  
M.N. Shalko ◽  
I.V. Malysheva ◽  
V.V. Chayka ◽  
...  
Keyword(s):  
Key Words ◽  
Assisted Reproductive Technologies ◽  
Cost Effective ◽  
Reproductive Technologies ◽  
Obstetric Complications ◽  
Reproductive Age ◽  
Cost Effective Method ◽  
Torch Infection ◽  
Positive Results ◽  
Torch Infections

The necessity of examining the Ukrainians of reproductive age on TORCH infection by using TORCH-IgG-test-MBA and TORCH-IgM-test-MBA test-kits has been analysed in this article. The study has been conducted to introduce an examination algorithm, which is supposed to increase the frequency of achieving positive results after using ART and reduce the frequency of perinatal and obstetric complications, as well as provide a quick and cost-effective method of infections diagnosis. Key words: infertility, assisted reproductive technologies, TORCH infections, test-kits.

scholarly journals Chromogenic Tube Test for Presumptive Identification or Confirmation of Isolates as Candida albicans

1998 ◽  
Vol 36 (4) ◽  
pp. 1157-1159 ◽  
Author(s):  
John Merlino ◽  
Evanthia Tambosis ◽  
Duncan Veal
Keyword(s):  
Candida Albicans ◽  
Sensitivity And Specificity ◽  
False Positive ◽  
Germ Tube ◽  
Cost Effective ◽  
Tube Test ◽  
Cost Effective Method ◽  
Presumptive Identification ◽  
Positive Results ◽  
Better Than

This report describes a new, modified, simple, and cost-effective method for the use of CHROMagar Candida (CHROMagar Company, Paris, France) for the presumptive identification of isolates as Candida albicans after preliminary growth. Sixty randomly selected clinical isolates were evaluated, including 38 of C. albicans. With incubation at 37°C for 24 h, the sensitivity and specificity appeared to be excellent and the test performed better than the traditional germ tube test. However, at earlier times, C. tropicalis isolates gave false-positive results.

CAD Of Anticorrosive Protection Of Steel Structures

2019 ◽  
pp. 18-23
Keyword(s):  
Protective Coating ◽  
Computer Aided Design ◽  
Steel Structures ◽  
Cost Effective ◽  
Operating Conditions ◽  
Computational Tool ◽  
Protection Method ◽  
Cost Effective Method ◽  
The Cost ◽  
Aided Design

The choice of cost-effective method of anticorrosive protection of steel structures is an urgent and time consuming task, considering the significant number of protection ways, differing from each other in the complex of technological, physical, chemical and economic characteristics. To reduce the complexity of solving this problem, the author proposes a computational tool that can be considered as a subsystem of computer-aided design and used at the stage of variant and detailed design of steel structures. As a criterion of the effectiveness of the anti-corrosion protection method, the cost of the protective coating during the service life is accepted. The analysis of existing methods of steel protection against corrosion is performed, the possibility of their use for the protection of the most common steel structures is established, as well as the estimated period of effective operation of the coating. The developed computational tool makes it possible to choose the best method of protection of steel structures against corrosion, taking into account the operating conditions of the protected structure and the possibility of using a protective coating.

Press “A” for Artificial Intelligence in Agriculture: A Review

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Yogesh Awasthi
Keyword(s):  
Artificial Intelligence ◽  
Image Analysis ◽  
Significant Role ◽  
Pattern Analysis ◽  
Cost Effective ◽  
Predictive Analysis ◽  
Accurate Information ◽  
Digital Era ◽  
Learning Techniques ◽  
Crucial Information

Agriculture is the backbone of the developing country. In old era agriculture was based on the experience which was shared by people to people but in this digital era technology play a very important and significant role in agriculture. Now agriculture become a business hub therefore farmers are focusing on precision farming. They introduced the technology in agriculture to define the accurate information about seed, soil, weather, disease and all factors which affecting the farming. Artificial Intelligence uses predictive analysis, image analysis, learning techniques and Pattern analysis to declare the best cost effective and maximum gain for the agriculturist. The aim of this paper is to provide the crucial information with the help of technology which a farmers can use to harvest the variety of crops as per the demand in world so that they can get maximum benefits.

Monitoring effluent quality in hypertrophic wastewater reservoirs using remote sensing

1996 ◽  
Vol 33 (8) ◽  
pp. 23-29 ◽  
Author(s):  
I. Dor ◽  
N. Ben-Yosef
Keyword(s):  
Water Quality ◽  
Theoretical Model ◽  
Principal Component ◽  
Cost Effective ◽  
Operational Parameters ◽  
Effluent Quality ◽  
Optical Images ◽  
Routine Monitoring ◽  
Cost Effective Method ◽  
Wastewater Quality

About one hundred and fifty wastewater reservoirs store effluents for irrigation in Israel. Effluent qualities differ according to the inflowing wastewater quality, the degree of pretreatment and the operational parameters. Certain aspects of water quality like concentration of organic matter, suspended solids and chlorophyll are significantly correlated with the water column transparency and colour. Accordingly optical images of the reservoirs obtained from the SPOT satellite demonstrate pronounced differences correlated with the water quality. The analysis of satellite multispectral images is based on a theoretical model. The model calculates, using the radiation transfer equation, the volume reflectance of the water body. Satellite images of 99 reservoirs were analyzed in the chromacity space in order to classify them according to water quality. Principal Component Analysis backed by the theoretical model increases the method sensitivity. Further elaboration of this approach will lead to the establishment of a time and cost effective method for the routine monitoring of these hypertrophic wastewater reservoirs.

A Cost-Effective Method to Prepare Pure Vildagliptin

2013 ◽  
Vol 10 (3) ◽  
pp. 159-163 ◽  
Author(s):  
Jun Peng ◽  
Yue Feng ◽  
Zhu Tao ◽  
Yingjie Chen ◽  
Xiangnan Hu
Keyword(s):  
Cost Effective ◽  
Cost Effective Method

scholarly journals Computer-supported Detection of M-Components and Evaluation of Immunoglobulins after Capillary Electrophoresis

2001 ◽  
Vol 47 (1) ◽  
pp. 110-117 ◽  
Author(s):  
Magnus Jonsson ◽  
Joyce Carlson ◽  
Jan-Olof Jeppsson ◽  
Per Simonsson
Keyword(s):  
Capillary Electrophoresis ◽  
Decision Support ◽  
Protein Separation ◽  
Serum Proteins ◽  
Cost Effective ◽  
Clinical Samples ◽  
Serum Samples ◽  
Computerized Decision Support ◽  
Cost Effective Method ◽  
Mathematical Algorithms

Abstract Background: Electrophoresis of serum samples allows detection of monoclonal gammopathies indicative of multiple myeloma, Waldenström macroglobulinemia, monoclonal gammopathy of undetermined significance, and amyloidosis. Present methods of high-resolution agarose gel electrophoresis (HRAGE) and immunofixation electrophoresis (IFE) are manual and labor-intensive. Capillary zone electrophoresis (CZE) allows rapid automated protein separation and produces digital absorbance data, appropriate as input for a computerized decision support system. Methods: Using the Beckman Paragon CZE 2000 instrument, we analyzed 711 routine clinical samples, including 95 monoclonal components (MCs) and 9 cases of Bence Jones myeloma, in both the CZE and HRAGE systems. Mathematical algorithms developed for the detection of monoclonal immunoglobulins (MCs) in the γ- and β-regions of the electropherogram were tested on the entire material. Additional algorithms evaluating oligoclonality and polyclonal concentrations of immunoglobulins were also tested. Results: CZE electropherograms corresponded well with HRAGE. Only one IgG MC of 1 g/L, visible on HRAGE, was not visible after CZE. Algorithms detected 94 of 95 MCs (98.9%) and 100% of those visible after CZE. Of 607 samples lacking an MC on HRAGE, only 3 were identified by the algorithms (specificity, 99%). Algorithms evaluating total gammaglobulinemia and oligoclonality also identified several cases of Bence Jones myeloma. Conclusions: The use of capillary electrophoresis provides a modern, rapid, and cost-effective method of analyzing serum proteins. The additional option of computerized decision support, which provides rapid and standardized interpretations, should increase the clinical availability and usefulness of protein analyses in the future.

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