scholarly journals Diagnostic Accuracy of Seven Commercial Assays for Rapid Detection of Group A Rotavirus Antigens

2015 ◽  
Vol 53 (11) ◽  
pp. 3670-3673 ◽  
Author(s):  
Jérôme Kaplon ◽  
Céline Fremy ◽  
Sylvie Pillet ◽  
Lucile Mendes Martins ◽  
Katia Ambert-Balay ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Real Time ◽  
Reverse Transcription ◽  
Acute Gastroenteritis ◽  
Rapid Detection ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A ◽  
Stool Samples ◽  
Human Stool

Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic rotavirus shedding identified by real-time reverse transcription-PCR.

Development of a real-time reverse transcription-PCR assay to detect and quantify group A rotavirus equine-like G3 strains

2021 ◽  
Author(s):  
Eric M. Katz ◽  
Mathew D. Esona ◽  
Rashi Gautam ◽  
Michael D. Bowen
Keyword(s):  
Viral Load ◽  
Real Time ◽  
Reverse Transcription ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Human Populations ◽  
Pcr Assay ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A

Since 2013, group A rotavirus strains characterized as novel DS-1-like inter-genogroup reassortant ‘equine-like G3’ strains have emerged and spread across five continents among human populations in at least 14 countries. Here we report a novel one-step TaqMan quantitative real-time reverse transcription-PCR assay developed to genotype and quantify the viral load for samples containing rotavirus equine-like G3 strains. Using a universal G forward primer and a newly designed reverse primer and TaqMan probe, we developed and validated an assay with a linear dynamic range of 2.3 × 10 9 – 227 copies per reaction and a limit of detection of 227 copies. The percent positive agreement, percent negative agreement, and precision of our assay were 100.00%, 99.63%, and 100.00%, respectively. This assay can simultaneously detect and quantify the viral load for samples containing DS-1-like inter-genogroup reassortant equine-like G3 strains with high sensitivity and specificity, faster turnaround time, and decreased cost and will be valuable for high-throughput screening of stool samples collected to monitor equine-like G3 strain prevalence and circulation among human populations throughout the world.

scholarly journals Evaluation of the ImmunoCardSTAT! Rotavirus Assay for Detection of Group A Rotavirus in Fecal Specimens

1999 ◽  
Vol 37 (6) ◽  
pp. 1977-1979 ◽  
Author(s):  
Penelope H. Dennehy ◽  
Michele Hartin ◽  
Sara M. Nelson ◽  
Shirley F. Reising
Keyword(s):  
Electron Microscopy ◽  
Positive Predictive Value ◽  
Reverse Transcription ◽  
Predictive Value ◽  
Rapid Detection ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A ◽  
Stool Specimens ◽  
Sensitivity Specificity

Rapid detection of group A rotavirus was performed by using ImmunoCardStat! Rotavirus (ICS-RV) (which uses immunogold-based, horizontal-flow membrane technology), two commercial enzyme immunoassays (Premier Rotaclone and TestPack Rotavirus), and electron microscopy. A total of 249 stool specimens collected from children with gastroenteritis between February and April 1997 were tested. After resolution of 19 of the 22 discordant results by reverse transcription-PCR for group A rotavirus, ICS-RV detected 125 positives while Rotaclone and TestPack detected 127 and 129 positives, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value were 94.0, 100, 100, and 93.4% for ICS-RV; 95.5, 100, 100, and 95.0% for Rotaclone; and 97.0, 96.5, 97.0, and 96.5% for TestPack. ICS-RV was sensitive and specific and was relatively simple to perform and interpret.

scholarly journals Prevalence of Group A Rotavirus, Human Calicivirus, Astrovirus, and Adenovirus Type 40 and 41 Infections among Children with Acute Gastroenteritis in Dijon, France

1999 ◽  
Vol 37 (9) ◽  
pp. 3055-3058 ◽  
Author(s):  
F. Bon ◽  
P. Fascia ◽  
M. Dauvergne ◽  
D. Tenenbaum ◽  
H. Planson ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Reverse Transcription ◽  
Acute Gastroenteritis ◽  
Adenovirus Type ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A Rotaviruses ◽  
Group A ◽  
Stool Specimens

Group A rotaviruses, human caliciviruses, astroviruses, and adenovirus types 40 and 41 were detected by enzyme immunoassay or reverse transcription-PCR in 61, 14, 6, and 3% of stool specimens from 414 children consulting for gastroenteritis between 1995 and 1998. These data highlight the importance of caliciviruses in infantile gastroenteritis. Among these, Norwalk-like viruses belonging to genogroup II were predominant.

scholarly journals Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: Correlation with clinical severity in children in South India

2004 ◽  
Vol 73 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Gagandeep Kang ◽  
Miren Iturriza-Gomara ◽  
Jeremy G. Wheeler ◽  
Premila Crystal ◽  
Bindhu Monica ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
South India ◽  
Clinical Severity ◽  
Chain Reaction ◽  
Group A Rotavirus ◽  
Group A ◽  
Polymerase Chain

scholarly journals Prevalence and genotype distribution of group A rotavirus circulating in Shanxi Province, China during 2015–2019

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lifeng Zhao ◽  
Xiaohong Shi ◽  
Dequan Meng ◽  
Jiane Guo ◽  
Yiping Li ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Shanxi Province ◽  
Nucleotide Sequencing ◽  
Genotype Distribution ◽  
Infection Frequency ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Rotavirus Vaccines ◽  
Infectious Gastroenteritis ◽  
Group A

Abstract Background Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children younger than 10 years of age with the diagnosis of acute gastroenteritis in Shanxi Province, China. Methods A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children’s Hospital of Shanxi from Jan 1, 2015, through Dec 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G- and P-genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. Results A total of 961 children younger than 10 years of age was enrolled over the study period, of whom 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12–23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1%), G2P[4] (3.3%), G1P[8] (0.5%) and G9P[4] (0.5%). Conclusions These results report for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.

scholarly journals Evaluation of immunochromatographic tests for the rapid detection of the emerging GII.17 norovirus in stool samples, January 2016

2016 ◽  
Vol 21 (4) ◽  
Author(s):  
Lucie Théry ◽  
Maxime Bidalot ◽  
Pierre Pothier ◽  
Katia Ambert-Balay
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Virus Titre ◽  
Chain Reaction ◽  
Diagnostic Assays ◽  
Stool Samples ◽  
Polymerase Chain

A novel GII.17 norovirus emerged in Asia in the winter of 2014/15. A worldwide spread is conceivable and norovirus diagnostic assays need to be evaluated to investigate if they adequately detect this emerging genotype. Seven immunochromatographic kits commercially available in Europe were evaluated on ten stool samples where GII.17 virus had been quantified by real-time reverse transcription-polymerase chain reaction. All the kits detected GII.17 with various sensitivities, partly depending on the virus titre.

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