scholarly journals Evaluation of the ImmunoCardSTAT! Rotavirus Assay for Detection of Group A Rotavirus in Fecal Specimens

1999 ◽  
Vol 37 (6) ◽  
pp. 1977-1979 ◽  
Author(s):  
Penelope H. Dennehy ◽  
Michele Hartin ◽  
Sara M. Nelson ◽  
Shirley F. Reising
Keyword(s):  
Electron Microscopy ◽  
Positive Predictive Value ◽  
Reverse Transcription ◽  
Predictive Value ◽  
Rapid Detection ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A ◽  
Stool Specimens ◽  
Sensitivity Specificity

Rapid detection of group A rotavirus was performed by using ImmunoCardStat! Rotavirus (ICS-RV) (which uses immunogold-based, horizontal-flow membrane technology), two commercial enzyme immunoassays (Premier Rotaclone and TestPack Rotavirus), and electron microscopy. A total of 249 stool specimens collected from children with gastroenteritis between February and April 1997 were tested. After resolution of 19 of the 22 discordant results by reverse transcription-PCR for group A rotavirus, ICS-RV detected 125 positives while Rotaclone and TestPack detected 127 and 129 positives, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value were 94.0, 100, 100, and 93.4% for ICS-RV; 95.5, 100, 100, and 95.0% for Rotaclone; and 97.0, 96.5, 97.0, and 96.5% for TestPack. ICS-RV was sensitive and specific and was relatively simple to perform and interpret.

scholarly journals Diagnostic Accuracy of Seven Commercial Assays for Rapid Detection of Group A Rotavirus Antigens

2015 ◽  
Vol 53 (11) ◽  
pp. 3670-3673 ◽  
Author(s):  
Jérôme Kaplon ◽  
Céline Fremy ◽  
Sylvie Pillet ◽  
Lucile Mendes Martins ◽  
Katia Ambert-Balay ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Real Time ◽  
Reverse Transcription ◽  
Acute Gastroenteritis ◽  
Rapid Detection ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A ◽  
Stool Samples ◽  
Human Stool

Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic rotavirus shedding identified by real-time reverse transcription-PCR.

scholarly journals Prevalence of Group A Rotavirus, Human Calicivirus, Astrovirus, and Adenovirus Type 40 and 41 Infections among Children with Acute Gastroenteritis in Dijon, France

1999 ◽  
Vol 37 (9) ◽  
pp. 3055-3058 ◽  
Author(s):  
F. Bon ◽  
P. Fascia ◽  
M. Dauvergne ◽  
D. Tenenbaum ◽  
H. Planson ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Reverse Transcription ◽  
Acute Gastroenteritis ◽  
Adenovirus Type ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A Rotaviruses ◽  
Group A ◽  
Stool Specimens

Group A rotaviruses, human caliciviruses, astroviruses, and adenovirus types 40 and 41 were detected by enzyme immunoassay or reverse transcription-PCR in 61, 14, 6, and 3% of stool specimens from 414 children consulting for gastroenteritis between 1995 and 1998. These data highlight the importance of caliciviruses in infantile gastroenteritis. Among these, Norwalk-like viruses belonging to genogroup II were predominant.

Development of a real-time reverse transcription-PCR assay to detect and quantify group A rotavirus equine-like G3 strains

2021 ◽  
Author(s):  
Eric M. Katz ◽  
Mathew D. Esona ◽  
Rashi Gautam ◽  
Michael D. Bowen
Keyword(s):  
Viral Load ◽  
Real Time ◽  
Reverse Transcription ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Human Populations ◽  
Pcr Assay ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Group A

Since 2013, group A rotavirus strains characterized as novel DS-1-like inter-genogroup reassortant ‘equine-like G3’ strains have emerged and spread across five continents among human populations in at least 14 countries. Here we report a novel one-step TaqMan quantitative real-time reverse transcription-PCR assay developed to genotype and quantify the viral load for samples containing rotavirus equine-like G3 strains. Using a universal G forward primer and a newly designed reverse primer and TaqMan probe, we developed and validated an assay with a linear dynamic range of 2.3 × 10 9 – 227 copies per reaction and a limit of detection of 227 copies. The percent positive agreement, percent negative agreement, and precision of our assay were 100.00%, 99.63%, and 100.00%, respectively. This assay can simultaneously detect and quantify the viral load for samples containing DS-1-like inter-genogroup reassortant equine-like G3 strains with high sensitivity and specificity, faster turnaround time, and decreased cost and will be valuable for high-throughput screening of stool samples collected to monitor equine-like G3 strain prevalence and circulation among human populations throughout the world.

scholarly journals Evaluation of the QiagenartusC. difficile QS-RGQ Kit for Detection of Clostridium difficile Toxins A and B in Clinical Stool Specimens

2015 ◽  
Vol 53 (6) ◽  
pp. 1942-1944 ◽  
Author(s):  
Nathalie Jazmati ◽  
Pia Wiegel ◽  
Božica Ličanin ◽  
Georg Plum
Keyword(s):  
Nucleic Acid ◽  
Clostridium Difficile ◽  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Content Type ◽  
Stool Specimens ◽  
Sensitivity Specificity

We compared the QiagenartusC. difficile QS-RGQ kit, a new nucleic acid amplification test for the detection ofClostridium difficiletoxins in stool specimens, with the Cepheid XpertC. difficiletest. The sensitivity, specificity, positive predictive value, and negative predictive value for the QiagenartusC. difficile QS-RGQ test were 100%, 89.5%, 60.9%, and 100%, and those for the Cepheid XpertC. difficiletest were 100%, 90%, 62.2%, and 100%, respectively.

scholarly journals Prevalence and genotype distribution of group A rotavirus circulating in Shanxi Province, China during 2015–2019

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lifeng Zhao ◽  
Xiaohong Shi ◽  
Dequan Meng ◽  
Jiane Guo ◽  
Yiping Li ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Shanxi Province ◽  
Nucleotide Sequencing ◽  
Genotype Distribution ◽  
Infection Frequency ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Rotavirus Vaccines ◽  
Infectious Gastroenteritis ◽  
Group A

Abstract Background Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children younger than 10 years of age with the diagnosis of acute gastroenteritis in Shanxi Province, China. Methods A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children’s Hospital of Shanxi from Jan 1, 2015, through Dec 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G- and P-genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. Results A total of 961 children younger than 10 years of age was enrolled over the study period, of whom 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12–23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1%), G2P[4] (3.3%), G1P[8] (0.5%) and G9P[4] (0.5%). Conclusions These results report for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.

scholarly journals Genotype Profiles of Rotavirus Strains from Children in a Suburban Community in Guinea-Bissau, Western Africa

2000 ◽  
Vol 38 (1) ◽  
pp. 264-267
Author(s):  
Thea Kølsen Fischer ◽  
Hans Steinsland ◽  
Kåre Mølbak ◽  
Rui Ca ◽  
Jon R. Gentsch ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Mixed Infections ◽  
Group A Rotavirus ◽  
Guinea Bissau ◽  
Reverse Transcription Pcr ◽  
Western Africa ◽  
Suburban Community ◽  
Group A ◽  
Unusual Type

ABSTRACT The P (VP4) and G (VP7) genotypes of 167 group A rotavirus strains obtained during the period 1996 to 1998 from 149 children living in a suburban community in Guinea-Bissau, western Africa, were determined by the reverse transcription-PCR technique. A total of nine combinations including five different P types and five different G types were identified. The globally common genotype pairs P[8], G1; P[4], G2; P[8], G3 and P[8], G4 were underrepresented in this study area. We found a substantial year-to-year variation in the occurrence of the genotype combinations. In 1996 and 1997, P[6], G2 was the most frequent, whereas P[8], G1 was more common in 1998. The unusual type P[9], G3 and a few mixed infections were detected. Sixteen percent of the rotavirus-positive samples were nontypeable.

scholarly journals Molecular Epidemiology of Human Group A Rotavirus Infections in the United Kingdom between 1995 and 1998

2000 ◽  
Vol 38 (12) ◽  
pp. 4394-4401 ◽  
Author(s):  
Miren Iturriza-Gómara ◽  
Jon Green ◽  
David W. G. Brown ◽  
Mary Ramsay ◽  
Ulrich Desselberger ◽  
...  
Keyword(s):  
United Kingdom ◽  
Molecular Epidemiology ◽  
Reverse Transcription ◽  
Animal Origin ◽  
Human Group ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
The United Kingdom ◽  
Group A ◽  
Rotavirus Infections

The G and P types of 2,912 rotavirus-positive fecal specimens collected from eight geographical areas of the United Kingdom between 1995 and 1998 were determined by reverse transcription-PCR. Although 15 different G-P combinations were identified, G1P[8], G2P[4], G3P[8], and G4P[8] strains constituted 95% of all the rotaviruses typed. Other genotypes included G9P[6] and G9P[8], which were first identified in the United Kingdom in 1995, or other uncommon G and/or P types of strains that may have had an animal origin. Unusual combinations of G1 or G4 with P[4] and G2 with P[8] which may have arisen by reassortment between human strains were also identified. G1P[8] was the genotype most frequently found (57 to 87%) in each season, followed by G2P[4] in the 1995–1996 (18%) and 1997–1998 (16%) seasons, although the incidence of infection with this virus decreased significantly to 2% during the 1996–1997 season. Significant differences were seen in the distributions of G1P[8], G2P[4], and G9P[8] strains between children and adults, in the temporal distributions of G4P[8] and G9P[8] strains within a season, and in the geographical distributions of each of the four most common genotypes from one season to the next.

scholarly journals Prevalence and Genotype Distribution of Group A Rotavirus Circulating in Shanxi Province, China During 2015-2019

2020 ◽  
Author(s):  
Lifeng Zhao ◽  
Xiaohong Shi ◽  
Dequan Meng ◽  
Jiane Guo ◽  
Yiping Li ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Shanxi Province ◽  
Nucleotide Sequencing ◽  
Genotype Distribution ◽  
Infection Frequency ◽  
Group A Rotavirus ◽  
Reverse Transcription Pcr ◽  
Rotavirus Vaccines ◽  
Infectious Gastroenteritis ◽  
Group A

Abstract Background: Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children under ten years old with the diagnosis of gastroenteritis in Shanxi Province, China. Methods: A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children’s Hospital of Shanxi from January 1, 2015, through December 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G and P genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. Results：A total of 961 archived stool specimens was examined, 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12–23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1 %), G2P[4] (3.3 %), G1P[8] (0.5 %) and G9P[4] (0.5 %).Conclusions: These results reported for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.

scholarly journals Distribution of Human Rotavirus G Types Circulating in Paris, France, during the 1997–1998 Epidemic: High Prevalence of Type G4

1999 ◽  
Vol 37 (7) ◽  
pp. 2373-2375 ◽  
Author(s):  
Elyanne Gault ◽  
Roxane Chikhi-Brachet ◽  
Sandrine Delon ◽  
Nathalie Schnepf ◽  
Laurence Albiges ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Children's Hospital ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus ◽  
High Prevalence ◽  
Children’S Hospital ◽  
Group A ◽  
Stool Specimens

Group A human rotavirus G genotypes were determined by means of reverse transcription-PCR in 170 stool specimens from children with acute diarrhea admitted to a Paris children’s hospital during a 1-year survey (1997 to 1998). The isolates all belonged to types G1 to G4, with type G4 predominating (60%).

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