Preparation of typing antisera specific for O antigens of Pseudomonas aeruginosa

Results of serotyping 966 clinical isolates of Pseudomonas aeruginosa showed that 72% agglutinated specifically in one or another of the 16 typing antisera, but 28% agglutinated in two or more and often in as many as 10 antisera; this polyagglutinability correlated with a high incidence of cross-reactivity among the antisera. Absorption of each typing antiserum with either cell suspensions of five O-type strains or with a suspension of a particular polyagglutinable strain (SMC 247) abolished cross-reactivity in the typing antisera without significantly reducing titers against the homologous strains. All but four of the polyagglutinable strains agglutinated specifically in one or another absorbed antisera. The cross-reactions of unabsorbed antisera were interpreted to have been caused by antibodies directed not against specific O antigens but against thermostable specificities that remain undefined.

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Serological studies ofBacteroides fragilis

Journal of Hygiene ◽

10.1017/s0022172400053043 ◽

1977 ◽

Vol 79 (2) ◽

pp. 233-241 ◽

Cited By ~ 15

Author(s):

K. M. Elhag ◽

K. A. Bettelheim ◽

Soad Tabaqchali

Keyword(s):

Bacteroides Fragilis ◽

Gram Negative ◽

Biochemical Characteristics ◽

Cross Reactions ◽

Homologous Antigen ◽

Specific Antisera ◽

Serological Studies ◽

The Cross ◽

O Antigens

SUMMARYUsing direct agglutination methods, a simple serological scheme for the classification ofBacteroides fragilisis described. Twenty strains ofB. fragiliswere selected by a process of successive screening from 151 strains obtained from various sources. O-antigens were prepared from the 20 strains, and used to raise antisera in rabbits.Each of the 20 antisera reacted with its homologous antigen and eight antisera cross-reacted with other subspecies. These cross-reactions were successfully removed after absorption of the antisera with the cross-reacting antigens, resulting in 19 type-specific antisera, titres ranging from 40 to 320, and 19 distinct serotypes ofB. fragilis. There was no correlation between the antigenic and the biochemical characteristics of these strains and no cross-reactions occurred with other gram-negative anaerobes,B. melaninogenicus, Sphaerophorus necrophorusandFuso-bacterium necrogenes.

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DETECTION OF IDIOTYPIC CROSS-REACTIONS AMONG STREPTOCOCCAL ANTISERA FROM RELATED RABBITS

Journal of Experimental Medicine ◽

10.1084/jem.138.3.522 ◽

1973 ◽

Vol 138 (3) ◽

pp. 522-537 ◽

Cited By ~ 42

Author(s):

Thomas J. Kindt ◽

Rochelle K. Seide ◽

Viktor A. Bokisch ◽

Richard M. Krause

Keyword(s):

Cross Reactivity ◽

Cross Reactions ◽

Group A ◽

The Cross

Idiotypic cross-reactions among antibodies to Group C streptococcal carbohydrate were studied using idiotypic antisera prepared in allotypically matched rabbits. Antibodies with idiotypic cross-specificity to one proband antibody were detected in 58% of the antisera from related rabbits, while approximately 1% of nonrelated rabbits produced antibody with this specificity. The cross-specificity was related to the group a (VH) allotype of 133 rabbits tested with only one exception. Studies utilizing antisera against a second proband antibody failed to detect antibodies with idiotypic cross-reactivity among the same group of related rabbits. This result emphasizes the variation in expression of idiotypic determinants of antibodies. It was further shown that the presence of anti-IgG's in the streptococcal antisera interfere with the detection of idiotypic cross-reactions. These anti-IgG's masked the presence of antibodies with idiotypic cross-specificity when inhibition of precipitation tests were used for their detection.

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Adhesion of Respiratory-Infection-Associated Microorganisms on Degradable Thermoplastic Composites

International Journal of Biomaterials ◽

10.1155/2009/765813 ◽

2009 ◽

Vol 2009 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Teemu Tirri ◽

Eva Söderling ◽

Minna Malin ◽

Matti Peltola ◽

Jukka V. Seppälä ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Streptococcus Pneumoniae ◽

Haemophilus Influenzae ◽

Bioactive Glass ◽

Respiratory Infection ◽

Bacterial Adhesion ◽

Clinical Isolates ◽

Thermoplastic Composites ◽

Type Strains

The purpose of this study was to evaluate bacterial adhesion and early colonization on a composite consisting of bioactive glass (BAG) particles and copolymer ofε-caprolactone/D,L-lactide. Materials were incubated with suspensions of both type strains and clinical isolates ofStreptococcus pneumoniae, Haemophilus influenzae, andPseudomonas aeruginosafor 30 minutes (adhesion) and 4 hours (colonization). Clear differences exist in the microorganisms' ability to adhere on the experimental materials. However, the presence of BAG particles does not inhibit bacterial adhesion, but early colonization of the materials withP. aeruginosawas inhibited by the addition of 90–315 μm BAG particles.

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A study of the surface and somatic antigens ofBacteroides fragilis

Journal of Hygiene ◽

10.1017/s0022172400024918 ◽

1978 ◽

Vol 80 (3) ◽

pp. 439-449 ◽

Cited By ~ 10

Author(s):

K. M. Elhag ◽

Soad Tabaqchali

Keyword(s):

Bacteroides Fragilis ◽

Cell Suspensions ◽

Surface Structures ◽

Whole Cell ◽

Cross Reactions ◽

Specific Antisera ◽

Serological Studies ◽

O Antigens ◽

Somatic Antigens ◽

Do So

SummaryAntisera against live cultures of 20 strains ofBacteroides fragilisproduced in rabbits agglutinated homologous whole-cell as well as O-antigen suspensions with several cross-reactions. Pure specific antisera were produced by absorbing cross-reacting antigens. O-antisera reacted against O-antigens but failed to do so against most whole-cell suspensions, suggesting the presence of surface structures which might have blocked the reactions. The titres of the whole-cell antisera were not significantly higher than those of O-antisera, but the reactions were more definite and easier to read.This system may prove useful in serological studies ofB. fragilis.

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Lymphocyte transformation and serum agar gel diffusion of rabbits immunized against Neisseria gonorrhoeae and Neisseria catarrhalis

Canadian Journal of Microbiology ◽

10.1139/m73-174 ◽

1973 ◽

Vol 19 (9) ◽

pp. 1099-1102 ◽

Cited By ~ 1

Author(s):

Violet Esquenazi ◽

Murray M. Streitfeld

Keyword(s):

Neisseria Gonorrhoeae ◽

Lymphocyte Transformation ◽

Cross Reactivity ◽

Soluble Antigen ◽

Agar Gel ◽

Cross Reactions ◽

Lymphocyte Blastogenesis ◽

The Cross ◽

Gel Diffusion ◽

Soluble Fractions

Gonococci were found to possess a number of antigens capable of stimulating lymphocyte blastogenesis in immunized rabbits. Cross antigenicity existed with N. catarrhalis; lymphocyte cross-reactivity to certain of the gonococcal soluble fractions and to whole sonicates of catarrhalis organisms followed immunization with either species. With increased postimmunization time, cross-reactions tended to disappear. Immunoprecipitate studies of the sera of immunized animals also demonstrated the cross-reactivity of the two organisms; however, only two of the gonococcal-soluble antigen fractions were reactive in this system.

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An isoform of the Ole e 7 allergen assembled by proteomics could explain the cross-reactivity with pollen and food nsLTPs

10.26226/morressier.5acc8ad2d462b8028d89a829 ◽

2018 ◽

Author(s):

Carmen Oeo

Keyword(s):

Cross Reactivity ◽

The Cross

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Cephalosporin nitric oxide-donor prodrug DEA-C3D disperses biofilms formed by clinical cystic fibrosis isolates of Pseudomonas aeruginosa

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz378 ◽

2019 ◽

Vol 75 (1) ◽

pp. 117-125 ◽

Cited By ~ 8

Author(s):

Odel Soren ◽

Ardeshir Rineh ◽

Diogo G Silva ◽

Yuming Cai ◽

Robert P Howlin ◽

...

Keyword(s):

Nitric Oxide ◽

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Clinical Isolates ◽

Nitric Oxide Donor ◽

Confocal Laser ◽

Broth Microdilution Method

Abstract Objectives The cephalosporin nitric oxide (NO)-donor prodrug DEA-C3D (‘DiEthylAmin-Cephalosporin-3′-Diazeniumdiolate’) has been shown to initiate the dispersal of biofilms formed by the Pseudomonas aeruginosa laboratory strain PAO1. In this study, we investigated whether DEA-C3D disperses biofilms formed by clinical cystic fibrosis (CF) isolates of P. aeruginosa and its effect in combination with two antipseudomonal antibiotics, tobramycin and colistin, in vitro. Methods β-Lactamase-triggered release of NO from DEA-C3D was confirmed using a gas-phase chemiluminescence detector. MICs for P. aeruginosa clinical isolates were determined using the broth microdilution method. A crystal violet staining technique and confocal laser scanning microscopy were used to evaluate the effects of DEA-C3D on P. aeruginosa biofilms alone and in combination with tobramycin and colistin. Results DEA-C3D was confirmed to selectively release NO in response to contact with bacterial β-lactamase. Despite lacking direct, cephalosporin/β-lactam-based antibacterial activity, DEA-C3D was able to disperse biofilms formed by three P. aeruginosa clinical isolates. Confocal microscopy revealed that DEA-C3D in combination with tobramycin produces similar reductions in biofilm to DEA-C3D alone, whereas the combination with colistin causes near complete eradication of P. aeruginosa biofilms in vitro. Conclusions DEA-C3D is effective in dispersing biofilms formed by multiple clinical isolates of P. aeruginosa and could hold promise as a new adjunctive therapy to patients with CF.

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Pharmacodynamics of once- versus twice-daily dosing of nebulized amikacin in an in vitro Hollow-Fiber Infection Model against 3 clinical isolates of Pseudomonas aeruginosa

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2021.115329 ◽

2021 ◽

Vol 100 (2) ◽

pp. 115329

Author(s):

Aaron James Heffernan ◽

Fekade Bruck Sime ◽

Saiyuri Naicker ◽

Katherine Andrews ◽

David Ellwood ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Hollow Fiber ◽

Clinical Isolates ◽

Infection Model

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Food antigen-specific IgE in dogs with suspected food hypersensitivity

Tierärztliche Praxis Ausgabe K Kleintiere / Heimtiere ◽

10.1055/a-1274-9210 ◽

2020 ◽

Vol 48 (06) ◽

pp. 395-402

Author(s):

Sandra A. Baumann ◽

Cornelius Fritz ◽

Ralf S. Mueller

Keyword(s):

Clinical Relevance ◽

Specific Ige ◽

Elimination Diet ◽

Cross Reactivity ◽

Food Hypersensitivity ◽

Strong Correlations ◽

Odds Ratios ◽

Cross Reactions ◽

Ige Reactivity ◽

Food Specific Ige

Abstract Objective Knowledge of cross-reactions in food-sensitive dogs will influence the choice of elimination diets and the long-term management of those patients. The objective of this study was to evaluate food allergen-specific IgE tests of suspected allergic dogs for concurrent positive reactions as possible evidence for cross reactions between allergens. Material and methods Results of serum IgE tests from 760 suspected allergic dogs submitted to 2 laboratories were evaluated statistically. After the tested allergens were grouped by their phylogenetic relationship, odds ratios as well as a sensitivity analysis of the odds ratios were performed to evaluate if concurrent positive IgE results to 2 allergens occurred more often than expected. Results Within related allergen pairs 27% (laboratory 1) and 72% (laboratory 2) of the pairs could be considered as associated. For the unrelated allergen pairs only 6.8% and 10.6% of the analyzed pairs were considered associated respectively. Strong correlations were shown in the group of ruminant allergens, especially beef and lamb, and grain allergens. High rates of concurrent reactions were also detected in the poultry group, especially between chicken and duck, as well as between pork and ruminant allergens, and soy and grain allergens. Conclusion As our results showed not only correlations within related but also between non-related allergens, the possible relevance of carbohydrate moieties as well as panallergens for canine hypersensitivities warrants further study. Further investigations are necessary to distinguish co-sensitization from cross-reactions and determine the clinical relevance of food-specific IgE reactivity. Clinical relevance Due to possible cross reactivity related allergens, especially beef and lamb as well as grain allergens, should not be used for an elimination diet to avoid false results.

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