scholarly journals Human papillomavirus type 16 E5 protein affects cell-cell communication in an epithelial cell line.

1995 ◽  
Vol 69 (7) ◽  
pp. 4489-4494 ◽  
Author(s):  
I Oelze ◽  
J Kartenbeck ◽  
K Crusius ◽  
A Alonso
Keyword(s):  
Human Papillomavirus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Cell Communication ◽  
Epithelial Cell Line ◽  
Human Papillomavirus Type 16 ◽  
E5 Protein ◽  
Cell Cell

scholarly journals Androgen induces differentiation of a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line

2001 ◽  
Vol 170 (1) ◽  
pp. 287-296 ◽  
Author(s):  
MT Ling ◽  
KW Chan ◽  
CK Choo
Keyword(s):  
Human Papillomavirus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Specific Antigen ◽  
Telomerase Activity ◽  
Epithelial Cell Line ◽  
Prostate Cell ◽  
Human Papillomavirus 16 ◽  
Prostate Epithelial Cell ◽  
Prostate Epithelial Cells

Androgen signaling is crucial for the growth and development, as well as for tumorigenesis of the prostate. However, many of the prostate epithelial cell lines developed previously, either normal or tumorigenic, do not express androgen receptor (AR) or respond to androgen. In order to advance our understanding on how androgen signaling regulates the growth and the differentiation status, and affects tumorigenicity of the epithelial cell, we performed experiments on HPr-1, a prostate cell line recently immortalized from normal human prostate epithelial cells. In the present study, AR was stably transfected into HPr-1 cells by replication-defective retrovirus. Treatment of HPr-1AR cells with androgen resulted in cell differentiation and growth retardation accompanied with up-regulation of cytokeratins K8 and K18, prostate specific antigen, p21 and p27, and down-regulation of c-myc, bcl-2 and telomerase activity. Our results suggest that androgen promotes the process of differentiation in a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line which may reflect the normal effects of androgen on prostate cells.

Immortalization of Normal Adult Human Middle Ear Epithelial Cells Using a Retrovirus Containing the E6/E7 Genes of Human Papillomavirus Type 16

2002 ◽  
Vol 111 (6) ◽  
pp. 507-517 ◽  
Author(s):  
Sung-Kyun Moon ◽  
Derald E. Brackmann ◽  
Young-Myoung chun ◽  
Haa-Yung Lee ◽  
Johng S. Rhim ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Epithelial Cells ◽  
Cell Line ◽  
Otitis Media ◽  
Middle Ear ◽  
Cell Biology ◽  
New Drugs ◽  
Epithelial Cell Line ◽  
Human Papillomavirus Type 16 ◽  
Human Middle Ear

A human middle ear epithelial cell line (HMEEC-1) was established using human papillomavirus E6/E7 genes. HMEEC-1 has remained morphologically and phenotypically stable, even after 50 passages. The cells are anchorage-dependent and nontumorigenic when injected into nude mice. This cell line thus provides a new tool for the study of normal cell biology and the pathological processes associated with the epithelial cells of the middle ear in otitis media. HMEEC-1 will also be useful in the search for new drugs and biological agents for the treatment of otitis media.

Cell-Cell Contact Down-Regulates Expression of Membrane Type MetaIIoproteinase-1 (MT1-MMP) in a Mouse Mammary Gland Epithelial Cell Line

1997 ◽  
Vol 14 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Satomi S. Tanaka ◽  
Yukiyasu Mariko ◽  
Hidetoshi Mori ◽  
Junko Ishijima ◽  
Sumie Tachi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mouse Mammary Gland ◽  
Epithelial Cell Line ◽  
Cell Contact ◽  
Mammary Gland Epithelial Cell ◽  
Membrane Type ◽  
Cell Cell

scholarly journals Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.

1996 ◽  
Vol 135 (6) ◽  
pp. 1643-1654 ◽  
Author(s):  
S Islam ◽  
T E Carey ◽  
G T Wolf ◽  
M J Wheelock ◽  
K R Johnson
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Squamous Cell ◽  
Epithelial Cell Line ◽  
Squamous Epithelial Cell ◽  
Squamous Carcinoma Cells ◽  
E Cadherin ◽  
Cell Cell

E-cadherin is a transmembrane glycoprotein that mediates calcium-dependent, homotypic cell-cell adhesion and plays an important role in maintaining the normal phenotype of epithelial cells. Disruption of E-cadherin activity in epithelial cells correlates with formation of metastatic tumors. Decreased adhesive function may be implemented in a number of ways including: (a) decreased expression of E-cadherin; (b) mutations in the gene encoding E-cadherin; or (c) mutations in the genes that encode the catenins, proteins that link the cadherins to the cytoskeleton and are essential for cadherin mediated cell-cell adhesion. In this study, we explored the possibility that inappropriate expression of a nonepithelial cadherin by an epithelial cell might also result in disruption of cell-cell adhesion. We showed that a squamous cell carcinoma-derived cell line expressed N-cadherin and displayed a scattered fibroblastic phenotype along with decreased expression of E- and P-cadherin. Transfection of this cell line with antisense N-cadherin resulted in reversion to a normal-appearing squamous epithelial cell with increased E- and P-cadherin expression. In addition, transfection of a normal-appearing squamous epithelial cell line with N-cadherin resulted in downregulation of both E- and P-cadherin and a scattered fibroblastic phenotype. In all cases, the levels of expression of N-cadherin and E-cadherin were inversely related to one another. In addition, we showed that some squamous cell carcinomas expressed N-cadherin in situ and those tumors expressing N-cadherin were invasive. These studies led us to propose a novel mechanism for tumorigenesis in squamous epithelial cells; i.e., inadvertent expression of a nonepithelial cadherin.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

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