scholarly journals Reassortment and Insertion-Deletion Are Strategies for the Evolution of Influenza B Viruses in Nature

1999 ◽  
Vol 73 (9) ◽  
pp. 7343-7348 ◽  
Author(s):  
Jonathan A. McCullers ◽  
George C. Wang ◽  
Shiqin He ◽  
Robert G. Webster
Keyword(s):  
Phylogenetic Trees ◽  
The United States ◽  
Influenza Viruses ◽  
Evolutionary Relationships ◽  
Nucleotide Sequencing ◽  
Influenza B ◽  
Antigenic Analysis ◽  
The Past ◽  
Different Strains ◽  
Reassortant Viruses

ABSTRACT The evolution of influenza B viruses is poorly understood. Reassortment of influenza B viruses in nature as a means of genetic variation has not been considered to be a major contributor to their evolution. However, the current practice of assigning evolutionary relationships by antigenic analysis of the hemagglutinin of influenza B viruses would fail to detect reassortants. In this study, influenza B viruses isolated within the past 10 years from sites in the United States and China were studied by nucleotide sequencing of the hemagglutinin and neuraminidase genes and construction of phylogenetic trees to assess evolutionary relationships. A group of viruses represented by B/Houston/1/92 possess a hemagglutinin derived from a B/Yamagata/16/88-like strain and a neuraminidase derived from a B/Victoria/2/87-like strain. A second reassortment event between the hemagglutinin of a B/Yamagata/16/88-like virus closely related to the B/Beijing/184/93 strain and the neuraminidase of a B/Victoria/2/87-like strain is represented by a single virus, B/Memphis/3/93. The neuraminidase of the reassortant viruses is most closely related to that of B/Victoria/2/87-like viruses currently circulating in Nanchang, China. A pattern of insertions and deletions in the hemagglutinin and the neuraminidase of different strains of influenza B viruses is observed. Reassortment plays a role in the evolution of influenza B viruses and may necessitate a change in the methods used to assess and identify new influenza viruses.

scholarly journals Antigenic and genetic analyses of influenza type B viruses isolated in Russia, 1987–91

1993 ◽  
Vol 111 (3) ◽  
pp. 539-546 ◽  
Author(s):  
M. L. Hemphill ◽  
P. A. Rota ◽  
V. T. Ivanova ◽  
A. N. Slepushkin ◽  
A. P. Kendal
Keyword(s):  
United States ◽  
Sequence Analysis ◽  
Influenza Season ◽  
The United States ◽  
Evolutionary Relationships ◽  
Influenza B ◽  
Type B ◽  
Antigenic Analysis ◽  
Influenza Type ◽  
Reference Strains

SummaryFour influenza type B viruses isolated in Russia during periods of relatively low (1987–8) or high (1990–1) influenza B activity were characterized antigenically using a microneutralization assay. These isolates were antigenically similar to contemporary reference strains from either of two separate lineages represented by B/Victoria/2/87 and B/Yamagata/16/88. The evolutionary relationships of the variable portion of the haemagglutinin (HA1) genes of these viruses were determined by comparison with influenza B HA1 sequences previously obtained. The Isolate B/USSR/2/87, collected during the 1987–8 influenza season, was found to be closely related to viruses on the B/Victoria/2/87 lineage that circulated during the 1988–9 influenza season in the United States. Sequence analysis of the isolates from the 1990–1 influenza season demonstrated co-circulation of viruses from both the B/Victoria/2/87 and B/Yamagata/16/88 lineages in Russia, confirming the antigenic analysis.

scholarly journals Live influenza B vaccine in volunteers

1969 ◽  
Vol 67 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A. S. Beare ◽  
D. A. J. Tyrrell ◽  
D. Hobson ◽  
C. H. L. Howells ◽  
M. S. Pereira ◽  
...  
Keyword(s):  
Research Unit ◽  
Vaccine Virus ◽  
Influenza Viruses ◽  
Influenza B ◽  
Serological Response ◽  
Antigenic Analysis ◽  
The Public ◽  
The Face ◽  
The Common ◽  
Resistance To Infection

SUMMARYA trial of an experimental live influenza B vaccine has been described.The virus it contained was active and produced infections, antibody rises and clinical reactions.Second and third vaccinations had much less effect than the first. Resistance to revaccination was only partially reflected in the serological response.It seems that another factor, probably local antibody, exerts a considerable influence on resistance to infection with influenza viruses.We are greatly indebted to Dr P. G. Higgins of the Public Health Laboratory, Cirencester, who went to much trouble to provide us with specimens from patients with influenza; to Dr H. G. Pereira of the National Institute for Medical Research for the antigenic analysis of the vaccine virus; to Messrs Sankey Ltd., Bilston, Wolverhampton, for their unfailing courtesy and forbearance throughout the trial; to the volunteers for their enthusiastic co-operation in the face of some discom forts; and to Messrs Pfizer Ltd., Sandwich, for originally providing facilities for the preparation of the vaccine.In particular we wish to record our gratitude for the invaluable technical help of Miss Pamela Ball of the Common Cold Research Unit, Mrs Maria Gregory of the Bacteriology Department of Liverpool University, Mrs L. Johnson of the Virus Laboratory of New Cross Hospital, Wolverhampton, and Mr A. Westoby, an assistant in the practice of Dr Tyler.

scholarly journals Circulating influenza viruses and the effectiveness of seasonal influenza vaccine in Romania, season 2012-2013 / Virusurile gripale circulante și eficacitatea vaccinului gripal sezonier în România, în sezonul 2012-2013

2015 ◽  
Vol 23 (1) ◽  
Author(s):  
Daniela Pitigoi ◽  
George Necula ◽  
Viorel Alexandrescu ◽  
Maria Elena Mihai ◽  
Carmen Maria Cherciu ◽  
...  
Keyword(s):  
Influenza Vaccine ◽  
Vaccine Strain ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Influenza Viruses ◽  
Nucleotide Sequencing ◽  
Influenza B ◽  
Seasonal Influenza Vaccine ◽  
Negative Case ◽  
Influenza A H1n1

AbstractBackgound. Using influenza epidemiological and virological surveillance data, we aimed at investigating the profile of influenza viruses circulating in Romania during the season 2012-2013 and estimating the effectiveness (VE) of the seasonal vaccine. Methods. We tested all specimens collected from patients with influenza like illness (ILI) in the national surveillance system between week 40/2012 to week 20/2013. Influenza A/B positive specimens identified by molecular detection (RT-PCR) were further characterized. We used hemagglutination inhibition assay for antigenic characterization and chemiluminiscence assay for the antiviral susceptibility testing. Subsequently we conducted nucleotide sequencing of hemagglutinin and neuraminidase genes and phylogenetic tree analyses. We estimated influenza VE using the test negative case-control study design, as 1-odds ratio of vaccination among ILI cases positive for influenza and ILI negative controls. Results and Discussions. We tested 1087 specimens, and 537 cases were positive (56.2% influenza B, 40.6% A(H1N1)pdm09, 3.2% A(H3N2). Sixty-four influenza viruses were antigenically and/or genetically characterized. A(H1N1)pdm09 viruses were related to the vaccine strain A/ California/07/2009 and clustered with genetic group 6 similar to A/St. Petersburg/27/2011. Influenza B viruses belonged to clade 2 of type B/Yamagata lineage, related to B/Estonia/55669/2011 except one, B/Victoria lineage, representative strain B/Brisbane/60/2008. A(H3) viruses clustered with group 3C of the A/Victoria/208/2009 clade, similar to the vaccine strain A/Victoria/361/2011. All tested strains (57) demonstrated susceptibility to oseltamivir and zanamivir. The adjusted seasonal influenza vaccine effectiveness against influenza A(H1N1)pdm09 (N=119) was 76.9% (95% CI: -113.4, 98.5), suggesting a good protection, consistent with the good match between the vaccine and circulating strains.

scholarly journals Evolution of Swine H3N2 Influenza Viruses in the United States

2000 ◽  
Vol 74 (18) ◽  
pp. 8243-8251 ◽  
Author(s):  
Richard J. Webby ◽  
Sabrina L. Swenson ◽  
Scott L. Krauss ◽  
Philip J. Gerrish ◽  
Sagar M. Goyal ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Influenza Viruses ◽  
H3n2 Virus ◽  
Reassortant Virus ◽  
Serum Samples ◽  
Internal Gene ◽  
H3n2 Influenza ◽  
Reassortant Viruses ◽  
The U.S

ABSTRACT During 1998, severe outbreaks of influenza were observed in four swine herds in the United States. This event was unique because the causative agents, H3N2 influenza viruses, are infrequently isolated from swine in North America. Two antigenically distinct reassortant viruses (H3N2) were isolated from infected animals: a double-reassortant virus containing genes similar to those of human and swine viruses, and a triple-reassortant virus containing genes similar to those of human, swine, and avian influenza viruses (N. N. Zhou, D. A. Senne, J. S. Landgraf, S. L. Swenson, G. Erickson, K. Rossow, L. Liu, K.-J. Yoon, S. Krauss, and R. G. Webster, J. Virol. 73:8851–8856, 1999). Because the U.S. pig population was essentially naive in regard to H3N2 viruses, it was important to determine the extent of viral spread. Hemagglutination inhibition (HI) assays of 4,382 serum samples from swine in 23 states indicated that 28.3% of these animals had been exposed to classical swine-like H1N1 viruses and 20.5% had been exposed to the triple-reassortant-like H3N2 viruses. The HI data suggested that viruses antigenically related to the double-reassortant H3N2 virus have not become widespread in the U.S. swine population. The seroreactivity levels in swine serum samples and the nucleotide sequences of six additional 1999 isolates, all of which were of the triple-reassortant genotype, suggested that H3N2 viruses containing avian PA and PB2 genes had spread throughout much of the country. These avian-like genes cluster with genes from North American avian viruses. The worldwide predominance of swine viruses containing an avian-like internal gene component suggests that these genes may confer a selective advantage in pigs. Analysis of the 1999 swine H3N2 isolates showed that the internal gene complex of the triple-reassortant viruses was associated with three recent phylogenetically distinct human-like hemagglutinin (HA) molecules. Acquisition of HA genes from the human virus reservoir will significantly affect the efficacy of the current swine H3N2 vaccines. This finding supports continued surveillance of U.S. swine populations for influenza virus activity.

scholarly journals MONOCLONAL ANTIBODIES TO HEMAGGLUTININ OF INFLUENZA B VIRUSES VICTORIA EVOLUTIONARY LINEAGE

2018 ◽  
Vol 63 (6) ◽  
pp. 275-280
Author(s):  
E. V. Sorokin ◽  
T. R. Tsareva ◽  
A. I. Zheltukhina
Keyword(s):  
Influenza Virus ◽  
Hemagglutination Inhibition ◽  
Influenza A ◽  
Rapid Identification ◽  
Influenza Viruses ◽  
Influenza B ◽  
Influenza A Viruses ◽  
Antigenic Analysis ◽  
Epidemic Season ◽  
Evolutionary Lineage

Co-circulation of two evolutionary distinct lineages of influenza virus in one epidemic season has led to development specific reagents for rapid identification and typing of new isolates. Panel of MAbs to hemagglutinin of influenza virus B/Brisbane/46/15 belonging to Victoria evolutionary lineage was developed. All MAbs reacted in ELISA with B/Victoria-like strains only. There were no interactions with heterologous influenza viruses of B/Yamagata lineage, seasonal and potentially pandemic influenza A viruses. All MAbs reacted in hemagglutination inhibition and virus neutralization. MAbs interacted in hemagglutination inhibition only with B/Victoria-like viruses, but did not interacted B/Yamagata-like strains. Neutralization and hemagglutination inhibition studies of viruses isolated before 1983 with MAbs revealed that MAbs 6E11, 9G5, 9B5 and 6A4 had the ability to interact with the virus B/ Russia/69 which may evidence that B strains of early isolation period (before lineage separation) have common epitope with recent Victoria lineage viruses. MAbs 7C8, 7G9, 7H8 and 8D11 were directed to a conserved epitope (or epitopes) specific for influenza hemagglutinin viruses of B/Victoria group. The presence of differences in the effectiveness of the interaction of MAbs 6A9, 7G9 and 8A8 in hemagglutination inhibition test allows the identification and differentiation of strains isolated in chicken embryos and MDCK cell culture. Thus, the developed MAbs can be successfully used for identification and antigenic analysis of B/Victoria-like strains.

scholarly journals Features of Epidemic Process of Influenza and its Etiology in the Countries of the Northern and Southern Hemispheres in the Period of Circulation of Pandemic Virus A(H1N1)pdm09 (According to WHO)

2020 ◽  
Vol 18 (6) ◽  
pp. 15-25
Author(s):  
L. S. Karpova ◽  
M. Yu. Pelikh ◽  
N. M. Popovtseva ◽  
T. P Stolyarova ◽  
K. M. Volik
Keyword(s):  
Influenza A ◽  
The United States ◽  
Influenza Viruses ◽  
Antigenic Structure ◽  
Influenza B ◽  
Tropical Countries ◽  
Antigenic Variability ◽  
Pdm09 Virus ◽  
Influenza A H1n1 ◽  
Northern And Southern Hemispheres

Relevance. Influenza is characterized by global distribution and the difference in its seasonality in countries with temperate and tropical climates. The importance of studying antigenic variation of influenza viruses due to the fact that changes in the antigenic structure is an evolutionary mechanism of adaptation of the virus to ensure its survival and cause annual epidemics.Aims. The Aim of this study was to identify the peculiarities of the geographical spread of influenza (seasonal), etiology and the rate of antigenic variability of influenza viruses A and B.Materials and methods. Based on data from WHO Reference research centers, information was collected on circulating influenza virus strains from 1975 A(H3N2), 1977 A(H1N1)pdm09 and type B of the Yamagata and Victoria lines from 1987 to 2019, as well as data on the number of all identified influenza viruses and individual strains circulating in the Northern and Southern hemispheres from 2008 to 2018.Results and discussion. Analysis of the global spread of influenza, its etiology and antigenic variability of viruses, according to WHO, showed that the influenza A(H1N1)pdm09 virus was the main causative agent of epidemics and regional outbreaks in seasons of high influenza activity in all countries except the United States and Canada, where influenza A(H3N2) and B viruses dominated in countries with severe seasonality, the change of season led to a change in the etiology of influenza, and in tropical countries, the A(H1N1)pdm09 virus more often remained dominant in all seasons of the year.Conclusions. The pronounced seasonality of influenza in Northern countries and its absence in tropical countries, where regional outbreaks prevailed in all seasons of the year, were confirmed. Low antigenic variability of influenza A(H1N1)pdm09 strains was confirmed, and the highest – A(H3N2). Among influenza B strains in the Victoria line had less antigenic variability, because the duration of its circulation before the appearance of a new drift variant was longer than that of the Yamagata line. The tendency to increase the total duration of circulation of influenza viruses B/Victoria, A(H1N1)pdm09 and B/Yamagata due to increased circulation before the emergence of new drift variants is shown.

International Research and Variation in Views of Ethnicity

2012 ◽  
Vol 2 (2) ◽  
pp. 66-72
Author(s):  
Ella Inglebret ◽  
Amy Skinder-Meredith ◽  
Shana Bailey ◽  
Carla Jones ◽  
Ashley France
Keyword(s):  
United States ◽  
Classification System ◽  
International Research ◽  
The United States ◽  
Research Articles ◽  
Journal Articles ◽  
Research Participants ◽  
The Past ◽  
International Scale ◽  
Publication Patterns

The authors in this article first identify the extent to which research articles published in three American Speech-Language-Hearing Association (ASHA) journals included participants, age birth to 18 years, from international backgrounds (i.e., residence outside of the United States), and go on to describe associated publication patterns over the past 12 years. These patterns then provide a context for examining variation in the conceptualization of ethnicity on an international scale. Further, the authors examine terminology and categories used by 11 countries where research participants resided. Each country uses a unique classification system. Thus, it can be expected that descriptions of the ethnic characteristics of international participants involved in research published in ASHA journal articles will widely vary.

Proportion of Individuals With Past-Year Suicidal Ideation Who Attempted Suicide Over the Past 10 Years in the United States, and the Influence of Age and Sex

Crisis ◽  
2020 ◽  
pp. 1-5
Author(s):  
Shannon Lange ◽  
Courtney Bagge ◽  
Charlotte Probst ◽  
Jürgen Rehm
Keyword(s):  
United States ◽  
Suicidal Ideation ◽  
Random Effects ◽  
Attempted Suicide ◽  
Suicide Attempts ◽  
The United States ◽  
Individual Level ◽  
The Past ◽  
Meta Analyses ◽  
Age Category

Abstract. Background: In recent years, the rate of death by suicide has been increasing disproportionately among females and young adults in the United States. Presumably this trend has been mirrored by the proportion of individuals with suicidal ideation who attempted suicide. Aim: We aimed to investigate whether the proportion of individuals in the United States with suicidal ideation who attempted suicide differed by age and/or sex, and whether this proportion has increased over time. Method: Individual-level data from the National Survey on Drug Use and Health (NSDUH), 2008–2017, were used to estimate the year-, age category-, and sex-specific proportion of individuals with past-year suicidal ideation who attempted suicide. We then determined whether this proportion differed by age category, sex, and across years using random-effects meta-regression. Overall, age category- and sex-specific proportions across survey years were estimated using random-effects meta-analyses. Results: Although the proportion was found to be significantly higher among females and those aged 18–25 years, it had not significantly increased over the past 10 years. Limitations: Data were self-reported and restricted to past-year suicidal ideation and suicide attempts. Conclusion: The increase in the death by suicide rate in the United States over the past 10 years was not mirrored by the proportion of individuals with past-year suicidal ideation who attempted suicide during this period.

EDITORIAL: Migration and competitiveness: Japan and the United States

2013 ◽  
Vol 10 (2) ◽  
pp. 115-124
Author(s):  
Philip L. Martin
Keyword(s):  
United States ◽  
The United States ◽  
Variable Cost ◽  
Fixed Cost ◽  
Special Issue ◽  
Science And Engineering ◽  
The Past ◽  
Past Half Century ◽  
Key Sectors ◽  
Past Half

Japan and the United States, the world’s largest economies for most of the past half century, have very different immigration policies. Japan is the G7 economy most closed to immigrants, while the United States is the large economy most open to immigrants. Both Japan and the United States are debating how immigrants are and can con-tribute to the competitiveness of their economies in the 21st centuries. The papers in this special issue review the employment of and impacts of immigrants in some of the key sectors of the Japanese and US economies, including agriculture, health care, science and engineering, and construction and manufacturing. For example, in Japanese agriculture migrant trainees are a fixed cost to farmers during the three years they are in Japan, while US farmers who hire mostly unauthorized migrants hire and lay off workers as needed, making labour a variable cost.

Democratic Legitimacy

2011 ◽  
Author(s):  
Pierre Rosanvallon
Keyword(s):  
United States ◽  
The United States ◽  
Democratic Legitimacy ◽  
Political Leaders ◽  
Constitutional Courts ◽  
International Debate ◽  
The Past ◽  
Democratic Authority ◽  
Original Contribution ◽  
Western Democracies

It's a commonplace occurrence that citizens in Western democracies are disaffected with their political leaders and traditional democratic institutions. But this book argues that this crisis of confidence is partly a crisis of understanding. The book makes the case that the sources of democratic legitimacy have shifted and multiplied over the past thirty years and that we need to comprehend and make better use of these new sources of legitimacy in order to strengthen our political self-belief and commitment to democracy. Drawing on examples from France and the United States, the book notes that there has been a major expansion of independent commissions, NGOs, regulatory authorities, and watchdogs in recent decades. At the same time, constitutional courts have become more willing and able to challenge legislatures. These institutional developments, which serve the democratic values of impartiality and reflexivity, have been accompanied by a new attentiveness to what the book calls the value of proximity, as governing structures have sought to find new spaces for minorities, the particular, and the local. To improve our democracies, we need to use these new sources of legitimacy more effectively and we need to incorporate them into our accounts of democratic government. This book is an original contribution to the vigorous international debate about democratic authority and legitimacy.

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