scholarly journals Release of Vasoactive Cytokines by Antibody-Enhanced Dengue Virus Infection of a Human Mast Cell/Basophil Line

2000 ◽  
Vol 74 (15) ◽  
pp. 7146-7150 ◽  
Author(s):  
Christine A. King ◽  
Jean S. Marshall ◽  
Hashem Alshurafa ◽  
Robert Anderson
Keyword(s):  
Mast Cell ◽  
Virus Infection ◽  
Dengue Virus ◽  
Human Mast Cell ◽  
Dengue Virus Infection ◽  
Ku812 Cells ◽  
A Cell ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

ABSTRACT We report here the first demonstration of dengue virus infection and vasoactive cytokine response of a cell of the mast cell/basophil lineage. Infection of KU812 cells was dependent on dengue-specific antibody and gave rise to infectious virions. This antibody-enhanced dengue virus infection triggered a four- to fivefold increase in the release of interleukin-1β (IL-1β) and a modest increase for IL-6 but not for an alternate cytokine, granulocyte-macrophage colony-stimulating factor. The results suggest a potential role for mast cells/basophils in the pathogenesis of dengue virus-induced disease.

scholarly journals Dengue Virus Selectively Induces Human Mast Cell Chemokine Production

2002 ◽  
Vol 76 (16) ◽  
pp. 8408-8419 ◽  
Author(s):  
Christine A. King ◽  
Robert Anderson ◽  
Jean S. Marshall
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Virus Infection ◽  
Dengue Virus ◽  
Specific Antibody ◽  
Human Mast Cell ◽  
Dengue Virus Infection ◽  
Human Mast Cells ◽  
Virus Specific Antibody ◽  
Rantes Production

ABSTRACT Severe dengue virus infections usually occur in individuals who have preexisting anti-dengue virus antibodies. Mast cells are known to play an important role in host defense against several pathogens, but their role in viral infection has not yet been elucidated. The effects of dengue virus infection on the production of chemokines by human mast cells were examined. Elevated levels of secreted RANTES, MIP-1α, and MIP-1β, but not IL-8 or ENA-78, were observed following infection of KU812 or HMC-1 human mast cell-basophil lines. In some cases a >200-fold increase in RANTES production was observed. Cord blood-derived cultured human mast cells treated with dengue virus in the presence of subneutralizing concentrations of dengue virus-specific antibody also demonstrated significantly (P < 0.05) increased RANTES production, under conditions which did not induce significant degranulation. Chemokine responses were not observed when mast cells were treated with UV-inactivated dengue virus in the presence or absence of human dengue virus-specific antibody. Neither antibody-enhanced dengue virus infection of the highly permissive U937 monocytic cell line nor adenovirus infection of mast cells induced a RANTES, MIP-1α, or MIP-1β response, demonstrating a selective mast cell response to dengue virus. These results suggest a role for mast cells in the initiation of chemokine-dependent host responses to dengue virus infection.

scholarly journals RNA Sensors Enable Human Mast Cell Anti-Viral Chemokine Production and IFN-Mediated Protection in Response to Antibody-Enhanced Dengue Virus Infection

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e34055 ◽  
Author(s):  
Michael G. Brown ◽  
Sarah M. McAlpine ◽  
Yan Y. Huang ◽  
Ian D. Haidl ◽  
Ayham Al-Afif ◽  
...  
Keyword(s):  
Mast Cell ◽  
Virus Infection ◽  
Dengue Virus ◽  
Human Mast Cell ◽  
Dengue Virus Infection ◽  
Chemokine Production ◽  
Rna Sensors

A cell-free assay to estimate the neutralizing capacity of granulocyte–macrophage colony-stimulating factor autoantibodies

2010 ◽  
Vol 360 (1-2) ◽  
pp. 141-148 ◽  
Author(s):  
Shinya Urano ◽  
Chinatsu Kaneko ◽  
Takahito Nei ◽  
Natsuki Motoi ◽  
Ryushi Tazawa ◽  
...  
Keyword(s):  
Colony Stimulating Factor ◽  
Neutralizing Capacity ◽  
A Cell ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

scholarly journals Adhesion-independent synergy of monocytes and endothelial cells in cytokine production: regulation of IL-6 and GM–CSF production by PAF

1996 ◽  
Vol 5 (1) ◽  
pp. 56-61 ◽  
Author(s):  
C. Lacasse ◽  
S. Turcotte ◽  
D. Gingras ◽  
M. Rola-Pleszczynski
Keyword(s):  
Endothelial Cells ◽  
Cell Populations ◽  
Cell Contact ◽  
Gm Csf ◽  
Cytokine Levels ◽  
A Cell ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor ◽  
Csf Production

Co-Cultures of monocytes (MO) and endothelial cells (EC) were studied for their capacity to synergize in the production of interleukin-6 (IL-6) and granulocyte-macrophage colony-stimulating factor (GM–CSF), two cytokines potentially important in vascular physiopathology. Resting monocytes produced detectable amounts of IL-6 but no GM–CSF, whereas confluent EC produced significant quantities of GM–CSF, but minimal IL-6. In co-cultures without stimuli, additive synthesis of both cytokines was observed. When EC were pretreated, however, with either PAF, TNF or both stimuli, before addition of MO, synergistic production of IL-6 was observed. In contrast, GM–CSF production was not enhanced by coculture of monocytes with activated EC. When either cell population was fixed with paraformaldehyde or killed by freeze-thawing before addition to the co-culture, cytokine levels reverted to those produced by the unaffected population alone. On the other hand, separating the two cell populations by a cell-impermeable membrane in transwell cultures did not affect the synergistic production of the cytokines. Taken together, our data suggest that EC and MO can synergize in response to stimuli by producing IL-6 and that this synergy is dependent on the integrity of both cell populations, but independent of cell-cell contact.

scholarly journals Identification of a Putative Coreceptor on Vero Cells That Participates in Dengue 4 Virus Infection

2001 ◽  
Vol 75 (17) ◽  
pp. 7818-7827 ◽  
Author(s):  
José de Jesús Martı́nez-Barragán ◽  
Rosa M. del Angel
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Vero Cells ◽  
Host Cells ◽  
Cell Surface Receptor ◽  
Target Cells ◽  
Virus Binding ◽  
Dengue Virus Infection ◽  
Surface Receptor ◽  
A Cell

ABSTRACT Dengue virus infects target cells by attaching to a cell surface receptor through the envelope (E) glycoprotein, located on the surface of the viral membrane. On Vero and BHK cells, heparan sulfate (HS) moieties of proteoglycans are the receptors for dengue virus; however, additional proteins have also been described as putative dengue virus receptors on C6/36, HL60, and BM cells. HS can also act as a receptor for other types of viruses or as an attachment molecule for viruses that require additional host cell molecules to allow viral penetration. In this study we searched for molecules other than HS that could participate in dengue virus infection of Vero cells. Labeled dengue 4 virus bound with high affinity to two molecules of 74 and 44 kDa. Binding of dengue virus to the 74-kDa molecule was susceptible to protease and sodium periodate treatment and resistant to heparinase treatments. Lectins such as concanavalin A and wheat germ agglutinin prevented dengue virus binding to both the 74- and the 44-kDa protein in overlay assays, while phytohemagglutinin P did not affect binding, suggesting that carbohydrate residues (α-mannose orN-acetylglucosamine) are important in virus binding to host cells. Protease susceptibility, biotin labeling, and immunofluorescence with a polyclonal antibody raised against the 74-kDa protein consistently identified the protein on the surfaces of Vero cells. Moreover, the antibody against the 74-kDa protein was able to inhibit dengue virus infection. These data suggest that HS might serve as a primary receptor, probably concentrating virus particles on the surfaces of Vero cells, and then other molecules, such as the 74-kDa protein, might participate as coreceptors in viral penetration. The 74-kDa protein possibly constitutes part of a putative receptor complex for dengue virus infection of Vero cells.

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