DNA Integration by Ty Integrase in yku70Mutant Saccharomyces cerevisiae Cells
2000 ◽
Vol 20
(23)
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pp. 8836-8844
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ABSTRACT In the present work we examined nonhomologous integration of plasmid DNA in a yku70 mutant. Ten of 14 plasmids integrated as composite elements, including Ty sequences probably originating from erroneous strand-switching and/or priming events. Three additional plasmids integrated via Ty integrase without cointegrating Ty sequences, as inferred from 5-bp target site duplication and integration site preferences. Ty integrase-mediated integration of non-Ty DNA has never been observed in wild-type cells, although purified integrase is capable of using non-Ty DNA as a substrate in vitro. Hence our data implicate yKu70 as the cellular function preventing integrase from accepting non-Ty DNA as a substrate.
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1989 ◽
Vol 9
(10)
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pp. 4467-4472
1992 ◽
Vol 12
(9)
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pp. 4084-4092
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2004 ◽
Vol 78
(11)
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pp. 5848-5855
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1996 ◽
Vol 16
(4)
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pp. 1805-1812
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1986 ◽
Vol 6
(2)
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pp. 404-410
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1998 ◽
Vol 11
(11)
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pp. 1136-1141
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2008 ◽
Vol 28
(7)
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pp. 2380-2390
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