Accumulation of c-src mRNA is developmentally regulated in embryonic neural retina.

Accumulation of c-src mRNA gradually increased during early development of the neural retina in chicken embryos and reached a peak by days 11 to 13 of embryonic life. Thereafter, its amount declined to a low level which persisted also in adult retina. The early increase in c-src mRNA correlated inversely with the decrease in the amount of H3.2 replication histone mRNA and with the decline in the rate of cell growth. The accumulation profile of c-src mRNA corresponded to that of pp60c-src protein, suggesting that the latter is regulated at the level of transcription.

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Mesodermal subdivision along the mediolateral axis in chicken controlled by different concentrations of BMP-4

Development ◽

10.1242/dev.124.10.1975 ◽

1997 ◽

Vol 124 (10) ◽

pp. 1975-1984 ◽

Cited By ~ 27

Author(s):

A. Tonegawa ◽

N. Funayama ◽

N. Ueno ◽

Y. Takahashi

Keyword(s):

Early Development ◽

Molecular Mechanisms ◽

Lateral Plate Mesoderm ◽

Tgf Beta ◽

Presomitic Mesoderm ◽

Lateral Plate ◽

Chicken Embryos ◽

Cos Cells ◽

Low Level ◽

High Level

Molecular mechanisms by which the mesoderm is subdivided along the mediolateral axis in early chicken embryos have been studied. When the presomitic mesoderm (medial mesoderm) was transplanted into the lateral plate, the graft was transformed into lateral plate tissue, indicating that the primitive somite was not fully committed and that the lateral plate has a cue for mesodermal lateralization. Since the lateral plate expresses a high level of BMP-4 mRNA, a member of the TGF-beta family, we hypothesized that it is the molecule responsible for the lateralization of the somite. To test this, we transplanted COS cells producing BMP-4 into the presomitic region. Those cells locally prevented the presomitic cells from differentiating into somites, converting them instead into lateral plate mesoderm, which was revealed by expression of cytokeratin mRNA, a marker for the lateral plate. The effect was dependent on the level of effective BMP-4: with a high level of BMP-4, the somite was transformed completely to lateral plate; with a low level, the somite formed but was occupied by the lateral somitic component expressing cSim 1, a marker for the lateral somite. These results suggest that different thresholds of effective BMP-4 determine distinct subtypes of the mesoderm as a lateralizer during early development.

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Apoptosis Occurs during Early Development of the Bursa of Fabricius in Chicken Embryos

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b14-00489 ◽

2014 ◽

Vol 37 (12) ◽

pp. 1982-1985 ◽

Cited By ~ 3

Author(s):

Kota Makino ◽

Runa Omachi ◽

Hiroka Suzuki ◽

Koji Tomobe ◽

Tsuyoshi Kawashima ◽

...

Keyword(s):

Early Development ◽

Bursa Of Fabricius ◽

Chicken Embryos

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pp60c-src is developmentally regulated in the neural retina

Cell ◽

10.1016/0092-8674(84)90218-6 ◽

1984 ◽

Vol 36 (2) ◽

pp. 249-257 ◽

Cited By ~ 155

Author(s):

Laurie K. Sorge ◽

Barcey T. Levy ◽

Patricia F. Maness

Keyword(s):

Neural Retina ◽

Developmentally Regulated

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Changes in the expression of the human adenine nucleotide translocase isoforms condition cellular metabolic/proliferative status

Open Biology ◽

10.1098/rsob.150108 ◽

2016 ◽

Vol 6 (2) ◽

pp. 150108 ◽

Cited By ~ 6

Author(s):

Aleix Gavaldà-Navarro ◽

Teresa Mampel ◽

Octavi Viñas

Keyword(s):

Growth Factor ◽

Cell Growth ◽

Cancer Cells ◽

Hepg2 Cells ◽

Adenine Nucleotide ◽

Growth Arrest ◽

Adenine Nucleotide Translocase ◽

Mrna Levels ◽

Developmentally Regulated ◽

Growth Factor Deprivation

Human cells express four mitochondrial adenine nucleotide translocase (hANT) isoforms that are tissue-specific and developmentally regulated. hANT1 is mainly expressed in terminally differentiated muscle cells; hANT2 is growth-regulated and is upregulated in highly glycolytic and proliferative cells; and hANT3 is considered to be ubiquitous and non-specifically regulated. Here, we studied how the expression of hANT isoforms is regulated by proliferation and in response to metabolic stimuli, and examined the metabolic consequences of their silencing and overexpression. In HeLa and HepG2 cells, expression of hANT3 was upregulated by shifting metabolism towards oxidation or by slowed growth associated with contact inhibition or growth-factor deprivation, indicating that hANT3 expression is highly regulated. Under these conditions, changes in hANT2 mRNA expression were not observed in either HeLa or HepG2 cells, whereas in SGBS preadipocytes (which, unlike HeLa and HepG2 cells, are growth-arrest-sensitive cells), hANT2 mRNA levels decreased. Additionally, overexpression of hANT2 promoted cell growth and glycolysis, whereas silencing of hANT3 decreased cellular ATP levels, limited cell growth and induced a stress-like response. Thus, cancer cells require both hANT2 and hANT3, depending on their proliferation status: hANT2 when proliferation rates are high, and hANT3 when proliferation slows.

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Cell Growth Modulation of Human Cells Irradiated in Vitro with Low-Level Laser Therapy

Photomedicine and Laser Surgery ◽

10.1089/pho.2004.22.523 ◽

2004 ◽

Vol 22 (6) ◽

pp. 523-526 ◽

Cited By ~ 30

Author(s):

Maddalena Mognato ◽

Francesca Squizzato ◽

Francesca Facchin ◽

Lucio Zaghetto ◽

Luigi Corti

Keyword(s):

Cell Growth ◽

Laser Therapy ◽

Human Cells ◽

Low Level Laser Therapy ◽

Growth Modulation ◽

Low Level ◽

Low Level Laser ◽

Level Laser

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Cell death in the ventral region of the neural retina during the early development of the chick embryo eye

The Anatomical Record ◽

10.1002/ar.1092220308 ◽

1988 ◽

Vol 222 (3) ◽

pp. 272-281 ◽

Cited By ~ 31

Author(s):

Gervasio Martín-Partido ◽

Lucía Rodríguez-Gallaro ◽

Ignacio S. Alvarez ◽

Julio Navascués

Keyword(s):

Cell Death ◽

Chick Embryo ◽

Early Development ◽

Neural Retina ◽

Ventral Region

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Low-level Lead Exposure and Early Development in Socioeconomically Advantaged Urban Infants

Lead Exposure and Child Development ◽

10.1007/978-94-009-0847-5_21 ◽

1989 ◽

pp. 345-356 ◽

Cited By ~ 3

Author(s):

D. Bellinger ◽

A. Leviton ◽

C. Waternaux ◽

H. Needleman ◽

M. Rabinowitz

Keyword(s):

Early Development ◽

Lead Exposure ◽

Low Level

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Immunodetection of thiol proteinase levels in various populations of Artemia cysts and during development

Biochemistry and Cell Biology ◽

10.1139/o91-015 ◽

1991 ◽

Vol 69 (2-3) ◽

pp. 96-101 ◽

Cited By ~ 7

Author(s):

J. Lu ◽

A. H. Warner

Keyword(s):

Early Development ◽

Western Blotting ◽

Linear Correlation ◽

Cathepsin B ◽

Brine Shrimp ◽

Developmentally Regulated ◽

Western Blots ◽

Artemia Cysts ◽

Good Linear Correlation ◽

Thiol Proteinase

An immunodetection assay on Western blots has been used to determine the thiol proteinase content and composition in cysts from 12 populations of the brine shrimp Artemia. Our results showed no differences in the subunit composition of the thiol proteinase among cysts from eight bisexual strains and four parthenogenic strains, and confirmed an earlier finding that the proteinase is composed of two subunits of 25.9 and 31.5 kilodaltons. In contrast, we found that Artemia cysts from parthenogenic strains contain 17.1 ng/cyst of the thiol proteinase, while cysts from bisexual strains contain 8.2 ng/cyst of the thiol proteinase. Also, there was a good linear correlation (r = 0.863; p < 0.001) between the thiol proteinase content and cyst mass. Embryo fractionation experiments showed that 82% of the thiol proteinase was in the cytosol, while 14 and 4%, respectively, were in the nuclei/yolk platelets and mitochondria/lysosome fractions. Measurements of the thiol proteinase content of developing Artemia embryos showed that the proteinase content was relatively constant during early development, suggesting that the activity of the thiol proteinase gene(s) may be constitutive and not developmentally regulated in Artemia embryos.Key words: Artemia, proteinase, development, cathepsin B, Western blotting.

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Effets stimulateurs des oligogalacturonates sur l'excrétion de polysaccharides acides par la suspension cellulaire de Silène

Canadian Journal of Botany ◽

10.1139/b90-240 ◽

1990 ◽

Vol 68 (9) ◽

pp. 1833-1839

Author(s):

Françoise Marga ◽

Bernard Priem ◽

Henri Morvan

Keyword(s):

Cell Growth ◽

Key Words ◽

Cultured Cells ◽

Degree Of Polymerization ◽

Acidic Polysaccharide ◽

Silene Alba ◽

Physicochemical Mechanism ◽

Early Increase ◽

Culture Cycle ◽

Polysaccharide Secretion

The composition and the acidity of the exopolysaccharides excreted into the medium of suspension-cultured white campion cells (Silene alba (Miller) E.H.L. Krause) in presence of oligogalacturonates changed during the first hours after transfer into a new medium. The early increase in acidic polysaccharide secretion in the presence of oligogalacturonates fell quickly during the course of the culture cycle. This effect increased with the degree of polymerization of oligomers..The rapid nature of this response and the influence of size suggests a physicochemical mechanism. Furthermore, cell growth was partially inhibited by the oligogalacturonates. Key words: oligogalacturonates, cell growth, exopolysaccharides, Silene alba, suspension-cultured cells.

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