scholarly journals Complete Genome Sequences of Measles Virus Genotype D8 Isolates from South Korea in 2016

2019 ◽  
Vol 8 (29) ◽  
Author(s):  
You-Jin Kim ◽  
Hae Ji Kang ◽  
Su-Jin Kim ◽  
Hye Min Lee ◽  
Sung Soon Kim
Keyword(s):  
Genetic Diversity ◽  
South Korea ◽  
Complete Genome ◽  
Measles Virus ◽  
Virus Transmission ◽  
Wild Type ◽  
Genome Sequences ◽  
Virus Genotype

The complete genome sequences of three wild-type measles viruses (genotype D8) isolated from patients in South Korea were determined. These are the first reported complete genome sequences of measles viruses obtained from South Korea, and the availability of these sequences will improve our understanding of measles virus transmission and genetic diversity.

scholarly journals Complete Genome Sequences of Six Measles Virus Strains

2018 ◽  
Vol 6 (13) ◽  
Author(s):  
My V. T. Phan ◽  
Claudia M. E. Schapendonk ◽  
Bas B. Oude Munnink ◽  
Marion P. G. Koopmans ◽  
Rik L. de Swart ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Complete Genome ◽  
Measles Virus ◽  
Genetic Characterization ◽  
Critical Component ◽  
Wild Type ◽  
Genome Sequences ◽  
Virus Strains ◽  
Generation Sequencing

ABSTRACT Genetic characterization of wild-type measles virus (MV) strains is a critical component of measles surveillance and molecular epidemiology. We have obtained complete genome sequences of six MV strains belonging to different genotypes, using random-primed next generation sequencing.

scholarly journals Complete Genome Sequence of a Genotype 2B Rubella Virus Isolated in South Korea in 2015

2017 ◽  
Vol 5 (38) ◽  
Author(s):  
Hae Ji Kang ◽  
You-Jin Kim ◽  
Hye Min Lee ◽  
Jeong-Gu Nam ◽  
Sung Soon Kim
Keyword(s):  
Genetic Diversity ◽  
South Korea ◽  
Genome Sequence ◽  
Rubella Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Wild Type

ABSTRACT The complete genome sequence of the wild-type genotype 2B rubella virus RVi/Busan.KOR/10.15[2B], isolated from a patient in South Korea, was determined. The availability of this sequence will help in understanding the circulation of endemic rubella viruses, as well as their genetic diversity.

scholarly journals Complete Genome Characterization of Two Wild-Type Measles Viruses from Vietnamese Infants during the 2014 Outbreak

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Bas B. Oude Munnink ◽  
My V. T. Phan ◽  
Paul Kellam ◽  
Matthew Cotten ◽  
Keyword(s):  
Complete Genome ◽  
Measles Virus ◽  
Pediatric Patients ◽  
Wild Type ◽  
Genome Sequences ◽  
Genome Characterization ◽  
Measles Outbreak ◽  
Stool Samples ◽  
Virus Genomes

A large measles virus outbreak occurred across Vietnam in 2014. We identified and obtained complete measles virus genomes in stool samples collected from two diarrheal pediatric patients in Dong Thap Province. These are the first complete genome sequences of circulating measles viruses in Vietnam during the 2014 measles outbreak.

scholarly journals Full-length sequences of 11 hepatitis C virus genotype 2 isolates representing five subtypes and six unclassified lineages with unique geographical distributions and genetic variation patterns

2012 ◽  
Vol 93 (6) ◽  
pp. 1173-1184 ◽  
Author(s):  
Chunhua Li ◽  
Hong Cao ◽  
Ling Lu ◽  
Donald Murphy
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Complete Genome ◽  
Pcr Amplification ◽  
Full Length ◽  
African Origin ◽  
Genome Sequences ◽  
Virus Genotype ◽  
Variation Patterns ◽  
Genotype 2

In this study, we characterized full-length hepatitis C virus (HCV) genome sequences for 11 genotype 2 isolates. They were isolated from the sera of 11 patients residing in Canada, of whom four had an African origin. Full-length genomes, each with 18–25 overlapping fragments, were obtained by PCR amplification. Five isolates represent the first complete genomes of subtypes 2d, 2e, 2j, 2m and 2r, while the other six correspond to variants that do not group within any assigned subtypes. These sequences had lengths of 9508–9825 nt and each contained a single ORF encoding 3012–3106 aa. Predicted amino acids were carefully inspected and unique variation patterns were recognized, especially for a 2e isolate, QC64. Phylogenetic analysis of complete genome sequences provides evidence that there are a total of 16 subtypes, of which 11 have been described here. Co-analysis with 68 partial NS5B sequences also differentiated 18 assigned subtypes, 2a–2r, and eight additional lineages within genotype 2, which is consistent with the analysis of complete genome sequences. The data from this study will now allow 10 assigned subtypes and six additional lineages of HCV genotype 2 to have their full-length genomes defined. Further analysis with 2021 genotype 2 sequences available in the HCV database indicated that the geographical distribution of these subtypes is consistent with an African origin, with particular subtypes having spread to Asia and the Americas.

scholarly journals Complete Genome Sequences of Micrococcus luteus Strains NCCP 15687 and NCCP 16831, Isolated in South Korea

2020 ◽  
Vol 9 (9) ◽  
Author(s):  
Sanghyun Lee ◽  
Ye-Won An ◽  
Chi-Hwan Choi ◽  
Mi-Ran Yun ◽  
Suyeon Kim ◽  
...  
Keyword(s):  
South Korea ◽  
National Culture ◽  
Complete Genome ◽  
Genomic Dna ◽  
Micrococcus Luteus ◽  
Culture Collection ◽  
Genome Sequences ◽  
Blood Samples ◽  
Content Type

In this study, the complete genome sequences of Micrococcus luteus strains NCCP 15687 and NCCP 16831 were determined and deposited in the National Culture Collection for Pathogens (NCCP) of South Korea. Genomic DNA was isolated from blood samples from patients infected with M. luteus.

scholarly journals Norovirus Polymerase Fidelity Contributes to Viral Transmission In Vivo

mSphere ◽  
2016 ◽  
Vol 1 (5) ◽  
Author(s):  
Armando Arias ◽  
Lucy Thorne ◽  
Elsa Ghurburrun ◽  
Dalan Bailey ◽  
Ian Goodfellow
Keyword(s):  
Genetic Diversity ◽  
Virus Transmission ◽  
Viral Pathogenesis ◽  
High Fidelity ◽  
Wild Type ◽  
Replication Fidelity ◽  
Polymerase Fidelity ◽  
Natural Hosts ◽  
Insight Into

ABSTRACT Virus replication fidelity and hence the intrahost genetic diversity of viral populations are known to be intricately linked to viral pathogenesis and tropism as well as to immune and antiviral escape during infection. In this study, we investigated whether changes in replication fidelity can impact the ability of a virus to transmit between susceptible hosts by the use of a mouse model for norovirus. We show that a variant encoding a high-fidelity polymerase is transmitted less efficiently between mice than the wild-type strain. This constitutes the first experimental demonstration that the polymerase fidelity of viruses can impact transmission of infection in their natural hosts. These results provide further insight into potential reasons for the global emergence of pandemic human noroviruses that display alterations in the replication fidelity of their polymerases compared to nonpandemic strains. Intrahost genetic diversity and replication error rates are intricately linked to RNA virus pathogenesis, with alterations in viral polymerase fidelity typically leading to attenuation during infections in vivo. We have previously shown that norovirus intrahost genetic diversity also influences viral pathogenesis using the murine norovirus model, as increasing viral mutation frequency using a mutagenic nucleoside resulted in clearance of a persistent infection in mice. Given the role of replication fidelity and genetic diversity in pathogenesis, we have now investigated whether polymerase fidelity can also impact virus transmission between susceptible hosts. We have identified a high-fidelity norovirus RNA-dependent RNA polymerase mutant (I391L) which displays delayed replication kinetics in vivo but not in cell culture. The I391L polymerase mutant also exhibited lower transmission rates between susceptible hosts than the wild-type virus and, most notably, another replication defective mutant that has wild-type levels of polymerase fidelity. These results provide the first experimental evidence that norovirus polymerase fidelity contributes to virus transmission between hosts and that maintaining diversity is important for the establishment of infection. This work supports the hypothesis that the reduced polymerase fidelity of the pandemic GII.4 human norovirus isolates may contribute to their global dominance. IMPORTANCE Virus replication fidelity and hence the intrahost genetic diversity of viral populations are known to be intricately linked to viral pathogenesis and tropism as well as to immune and antiviral escape during infection. In this study, we investigated whether changes in replication fidelity can impact the ability of a virus to transmit between susceptible hosts by the use of a mouse model for norovirus. We show that a variant encoding a high-fidelity polymerase is transmitted less efficiently between mice than the wild-type strain. This constitutes the first experimental demonstration that the polymerase fidelity of viruses can impact transmission of infection in their natural hosts. These results provide further insight into potential reasons for the global emergence of pandemic human noroviruses that display alterations in the replication fidelity of their polymerases compared to nonpandemic strains.

scholarly journals Complete Genome Sequences of Weissella cibaria Strains CMU, CMS1, CMS2, and CMS3 Isolated from Infant Saliva in South Korea

2017 ◽  
Vol 5 (40) ◽  
Author(s):  
Mi-Sun Kang ◽  
Ji-Eun Yeu ◽  
Jong-Suk Oh ◽  
Boo-Ahn Shin ◽  
Jin-Hee Kim
Keyword(s):  
South Korea ◽  
Complete Genome ◽  
Oral Care ◽  
Genome Sequences ◽  
Content Type ◽  
Weissella Cibaria

ABSTRACT Weissella cibaria strain CMU is used as a commercial oral care probiotic in South Korea. Here, we present the complete genome sequences of four W. cibaria strains (CMU, CMS1, CMS2, and CMS3) isolated from the saliva of an infant living in Gwangju, South Korea.

scholarly journals Complete Genome Sequences of SevenHelicoverpa armigeraSNPV-AC53-Derived Strains

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Christopher Noune ◽  
Caroline Hauxwell
Keyword(s):  
Tissue Culture ◽  
Helicoverpa Armigera ◽  
Complete Genome ◽  
Full Genome ◽  
Wild Type ◽  
Genome Sequences ◽  
Helicoverpa Armígera ◽  
Multiple Strains

Wild-type baculovirus isolates typically consist of multiple strains. We report the full genome sequences of seven alphabaculovirus strains derived by passage through tissue culture fromHelicoverpa armigeraSNPV-AC53 (KJ909666).

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