scholarly journals Genomics of the Uncultivated, Periodontitis-Associated BacteriumTannerellasp. BU045 (Oral Taxon 808)

mSystems ◽  
2018 ◽  
Vol 3 (3) ◽  
Author(s):  
Clifford J. Beall ◽  
Alisha G. Campbell ◽  
Ann L. Griffen ◽  
Mircea Podar ◽  
Eugene J. Leys
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Chronic Periodontitis ◽  
Bacterial Species ◽  
The United States ◽  
Oral Microbiome ◽  
Rrna Gene ◽  
Bacterial Cells ◽  
Data Set ◽  
Content Type

ABSTRACTDespite decades of research into the human oral microbiome, many species remain uncultivated. The technique of single-cell whole-genome amplification and sequencing provides a means of deriving genome sequences for species that can be informative on biological function and suggest pathways to cultivation.Tannerella forsythiahas long been known to be highly associated with chronic periodontitis and to cause periodontitis-like symptoms in experimental animals, andTannerellasp. BU045 (human oral taxon 808) is an uncultivated relative of this organism. In this work, we extend our previous sequencing of theTannerellasp. BU063 (human oral taxon 286) genome by sequencing amplified genomes from 11 cells ofTannerellasp. BU045, including 3 genomes that are at least 90% complete.Tannerellasp. BU045 is more closely related toTannerellasp. BU063 than toT. forsythiaby gene content and average nucleotide identity. However, two independent data sets of association with periodontitis, one based on 16S rRNA gene abundance and the other based on gene expression in a metatranscriptomic data set, show thatTannerellasp. BU045 is more highly associated with disease thanTannerellasp. BU063. Comparative genomics shows genes and functions that are shared or unique to the different species, which may direct further research of the pathogenesis of chronic periodontitis.IMPORTANCEPeriodontitis (gum disease) affects 47% of adults over 30 in the United States (P. I. Eke, B. A. Dye, L. Wei, G. O. Thornton-Evans, R. J. Genco, et al., J Dent Res 91:914–920, 2012), and it cost between $39 and $396 billion worldwide in 2015 (A. J. Righolt, M. Jevdjevic, W. Marcenes, and S. Listl, J Dent Res, 17 January 2018, https://doi.org/10.1177/0022034517750572). Many bacteria associated with the disease are known only by the DNA sequence of their 16S rRNA gene. In this publication, amplification and sequencing of DNA from single bacterial cells are used to obtain nearly complete genomes ofTannerellasp. BU045, a species of bacteria that is more prevalent in patients with periodontitis than in healthy patients. Comparing the complete genome of this bacterium to genomes of related bacterial species will help to better understand periodontitis and may help to grow this organism in pure culture, which would allow a better understanding of its role in the mouth.

scholarly journals Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Mouse Feces

2015 ◽  
Vol 81 (19) ◽  
pp. 6749-6756 ◽  
Author(s):  
Yun-Wen Yang ◽  
Mang-Kun Chen ◽  
Bing-Ya Yang ◽  
Xian-Jie Huang ◽  
Xue-Rui Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Species ◽  
Chronic Inflammatory Bowel Disease ◽  
Trinitrobenzene Sulfonic Acid ◽  
Rrna Gene ◽  
Specific Primers ◽  
Content Type

ABSTRACTMouse models are widely used for studying gastrointestinal (GI) tract-related diseases. It is necessary and important to develop a new set of primers to monitor the mouse gut microbiota. In this study, 16S rRNA gene-targeted group-specific primers forFirmicutes,Actinobacteria,Bacteroidetes,Deferribacteres, “CandidatusSaccharibacteria,”Verrucomicrobia,Tenericutes, andProteobacteriawere designed and validated for quantification of the predominant bacterial species in mouse feces by real-time PCR. After confirmation of their accuracy and specificity by high-throughput sequencing technologies, these primers were applied to quantify the changes in the fecal samples from a trinitrobenzene sulfonic acid-induced colitis mouse model. Our results showed that this approach efficiently predicted the occurrence of colitis, such as spontaneous chronic inflammatory bowel disease in transgenic mice. The set of primers developed in this study provides a simple and affordable method to monitor changes in the intestinal microbiota at the phylum level.

scholarly journals Saezia sanguinis gen. nov., sp. nov., a Betaproteobacteria member of order Burkholderiales, isolated from human blood

2020 ◽  
Vol 70 (3) ◽  
pp. 2016-2025 ◽  
Author(s):  
María J. Medina-Pascual ◽  
Sara Monzón ◽  
Pilar Villalón ◽  
Isabel Cuesta ◽  
Fernando González-Romo ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Bacterial Cells ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

The taxonomic position of an unknown bacterial strain designated CNM695-12, isolated from the blood of an immunocompromised subject, was investigated via phenotypic, chemotaxonomic, genotypic and genomic analyses. Bacterial cells were determined to be Gram-stain-negative bacilli, aerobic, non-motile and non-spore-forming. The strain showed catalase activity but no oxidase activity. Optimal growth occurred at 37 °C, pH 7 and with 0–1 % NaCl. C16 : 0, summed feature 8 (comprising C18 : 1ω7c /C18:1 ω6c), and C18 : 1ω9c were the most abundant fatty acids, and ubiquinone 8 was the major respiratory quinone. The polar lipids present included phosphatidylglycerol, phosphatidylethanolamine and other aminophospholipids. The 16S rRNA gene sequence showed approximately 93.5 % similarity to those of different species with validly published names within the order Burkholderiales (e.g. Leptothrix mobilis Feox-1T, Aquabacterium commune B8T , Aquabacterium citratiphilum B4T and Schlegelella thermodepolymerans K14T). Phylogenetic analyses based on 16S rRNA gene sequences and concatenated alignments including the sequences for 107 essential proteins, revealed the strain to form a novel lineage close to members of the family Comamonadaceae . The highest average nucleotide identity and average amino acid identity values were obtained with Schlegelella thermodepolymerans K14T (69.6 and 55.7 % respectively). The genome, with a size of 3.35 Mb, had a DNA G+C content of 52.4 mol% and encoded 3056 predicted genes, 3 rRNA, 1 transfer–messengerRNA and 51 tRNA. Strain CNM695-12 thus represents a novel species belonging to a novel genus within the order Burkholderiales , for which the name Saezia sanguinis gen. nov., sp. nov. is proposed. The type strain is CNM695-12T (=DSM 104959T=CECT 9208T).

scholarly journals Bacterial Diversity Profiling of the New Zealand Parasitic Blowfly Lucilia sericata Based on 16S rRNA Gene Amplicon Sequencing

2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Nikola Palevich ◽  
Paul H. Maclean ◽  
Luis Carvalho ◽  
Ruy Jauregui
Keyword(s):  
New Zealand ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Diversity ◽  
Sequence Data ◽  
Amplicon Sequencing ◽  
Lucilia Sericata ◽  
Rrna Gene ◽  
Data Set ◽  
Content Type

ABSTRACT Here, we present a 16S rRNA gene amplicon sequence data set and profiles demonstrating the bacterial diversity of larval and adult Lucilia sericata, collected from Ashhurst, New Zealand (May 2020). The two dominant genera among adult male and female L. sericata were Serratia and Morganella (phylum Proteobacteria), while the larvae were also dominated by the genera Lactobacillus, Carnobacterium, and Lactococcus (phylum Firmicutes).

scholarly journals Characterization of Desulfovibrio biadhensis sp. nov., isolated from a thermal spring

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1256-1261 ◽  
Author(s):  
Khaled Fadhlaoui ◽  
Wajdi Ben Hania ◽  
Anne Postec ◽  
Guy Fauque ◽  
Moktar Hamdi ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Bacterial Species ◽  
Thermal Spring ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Comparisons ◽  
Content Type

A novel anaerobic, mesophilic, slightly halophilic sulfate-reducing bacterium, designated strain Khaled BD4T, was isolated from waters of a Tunisian thermal spring. Cells were vibrio-shaped or sigmoids (5–7×1–1.5 µm) and occurred singly or in pairs. Strain Khaled BD4T was Gram-stain-negative, motile and non-sporulated. It grew at 25–45 °C (optimum 37 °C), at pH 5.5–8.3 (optimum pH 7.0) and with 0.5–8 % NaCl (optimum 3 %). It required vitamins or yeast extract for growth. Sulfate, thiosulfate, sulfite and elemental sulfur served as terminal electron acceptors, but not fumarate, nitrate or nitrite. Strain Khaled BD4T utilized H2 in the presence of 2 mM acetate (carbon source), but also lactate, formate, pyruvate and fumarate in the presence of sulfate. Lactate was incompletely oxidized to acetate. Amongst substrates used, only pyruvate was fermented. Desulfoviridin and c-type cytochrome were present. The G+C content of the DNA was 54.6 mol%. The main fatty acids were anteiso-C15 : 0, iso-C18 : 0, iso-C17 : 0 and iso-C14 : 0. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Khaled BD4T had Desulfovibrio giganteus DSM 4123T (96.7 % similarity) as its closest phylogenetic relative. On the basis of 16S rRNA gene sequence comparisons together with genetic and physiological characteristics, strain Khaled BD4T is assigned to a novel bacterial species, for which the name Desulfovibrio biadhensis sp. nov. is proposed. The type strain is Khaled BD4T ( = DSM 28904T = JCM 30146T).

Chungangia koreensis gen. nov., sp. nov., isolated from marine sediment

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1914-1920 ◽  
Author(s):  
Wonyong Kim ◽  
Jitsopin Traiwan ◽  
Mi-Hak Park ◽  
Min Young Jung ◽  
Su-Jin Oh ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Sediment ◽  
Polar Lipid ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Content Type ◽  
Link Type

A Gram-staining-positive, strictly aerobic, non-spore-forming, rod-shaped bacterial strain, CAU 9163T, was isolated from marine sediment collected in the Republic of Korea and its taxonomic position was investigated using a polyphasic approach. The novel strain grew optimally at 30 °C and pH 8.0. In phylogenetic analysis based on 16S rRNA gene sequences, strain CAU 9163T formed a hitherto unknown lineage within the order Bacillales , which contains the genera Planomicrobium , Planococcus , Sporosarcina , Rummeliibacillus , Viridibacillus , Lysinibacillus and Bacillus . The levels of 16S rRNA gene sequence similarity between the novel strain and any established bacterial species were all <95.7 %. The major isoprenoid quinines of strain CAU 9163T were MK-8 (65.2 %) and MK-7 (22.8 %) and the predominant fatty acid was anteiso-C15 : 0. The peptidoglycan was of the A4α type and based on l-Lys-d-Asp. The major whole-cell sugars were ribose and glucose. The polar lipid profile mainly consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified polar lipid. The genomic DNA G+C content of the novel strain was 44.3 mol%. These data were sufficient to differentiate the novel strain from established genera in the phylum Firmicutes . Based on the phenotypic, chemotaxonomic and genotypic evidence, strain CAU 9163T represents a novel species in a new genus for which the name Chungangia koreensis gen. nov., sp. nov. is proposed. The type strain of Chungangia koreensis is 9163T ( = KCTC 13729T  = CCUG 59778T).

scholarly journals A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

mSystems ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Susan Joseph ◽  
Joseph Aduse-Opoku ◽  
Ahmed Hashim ◽  
Eveliina Hanski ◽  
Ricarda Streich ◽  
...  
Keyword(s):  
16S Rrna ◽  
Mouse Model ◽  
16S Rrna Gene ◽  
Oral Microbiome ◽  
Easy Access ◽  
Rrna Gene ◽  
Laboratory Mice ◽  
Wild Mice ◽  
Content Type ◽  
Model Studies

ABSTRACT A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology. IMPORTANCE Mouse model studies are frequently used in oral microbiome research, particularly to investigate diseases such as periodontitis and caries, as well as other related systemic diseases. We have reported here the details of the development of a curated reference database to characterize the oral microbial community in laboratory and some wild mice. The genomic information and findings reported here can help improve the outcomes and accuracy of host-microbe experimental studies that use murine models to understand health and disease. Work is also under way to make the reference data sets publicly available on a web server to enable easy access and downloading for researchers across the world.

scholarly journals Caproicibacter fermentans gen. nov., sp. nov., a new caproate-producing bacterium and emended description of the genus Caproiciproducens

2020 ◽  
Vol 70 (7) ◽  
pp. 4269-4279 ◽  
Author(s):  
Maximilian Flaiz ◽  
Tina Baur ◽  
Sven Brahner ◽  
Anja Poehlein ◽  
Rolf Daniel ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Link Type ◽  
The Family

A strictly anaerobic bacterial strain designated EA1T was isolated from an enrichment culture inoculated with biogas reactor content. Cells of strain EA1T are spore-forming rods (1–3×0.4–0.8 µm) and stain Gram-negative, albeit they possess a Gram-positive type of cell-wall ultrastructure. Growth of strain EA1T was observed at 30 and 37 °C and within a pH range of pH 5–9. The major components recovered in the fatty acid fraction were C14:0, C16:0, C16:0 DMA (dimethyl acetal) and C16:1 ω7c. Strain EA1T fermented several mono- and disaccharides. Metabolic end products from fructose were acetate, butyrate, caproate and lactate. Furthermore, ethanol, CO2 and H2 were identified as products. The genome consists of a chromosome (3.9 Mbp) with 3797 predicted protein-encoding genes and a G+C content of 51.25 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EA1T represents a novel taxon within the family Oscillospiraceae . The most closely related type strains of EA1T, based on 16S rRNA gene sequence identity, are Caproiciproducens galactitolivorans BS-1T (94.9 %), [ Clostridium ] leptum DSM 753T (93.8 %), [ Clostridium ] sporosphaeroides DSM 1294T (91.7 %) and Ruminococcus bromii ATCC 27255T (91.0 %). Further phenotypic characteristics of strain EA1T differentiate it from related, validly described bacterial species. Strain EA1T represents a novel genus and novel species within the family Oscillospiraceae . The proposed name is Caproicibacter fermentans gen. nov., sp. nov. The type strain is EA1T (DSM 107079T=JCM 33110T).

scholarly journals New Insights into Human Nostril Microbiome from the Expanded Human Oral Microbiome Database (eHOMD): a Resource for the Microbiome of the Human Aerodigestive Tract

mSystems ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Isabel F. Escapa ◽  
Tsute Chen ◽  
Yanmei Huang ◽  
Prasad Gajare ◽  
Floyd E. Dewhirst ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Clinical Relevance ◽  
Species Level ◽  
Aerodigestive Tract ◽  
Oral Microbiome ◽  
Rrna Gene ◽  
Content Type ◽  
Nasal Passages ◽  
Vital Resource

ABSTRACT The expanded Human Oral Microbiome Database (eHOMD) is a comprehensive microbiome database for sites along the human aerodigestive tract that revealed new insights into the nostril microbiome. The eHOMD provides well-curated 16S rRNA gene reference sequences linked to available genomes and enables assignment of species-level taxonomy to most next-generation sequences derived from diverse aerodigestive tract sites, including the nasal passages, sinuses, throat, esophagus, and mouth. Using minimum entropy decomposition coupled with the RDP Classifier and our eHOMD V1-V3 training set, we reanalyzed 16S rRNA V1-V3 sequences from the nostrils of 210 Human Microbiome Project participants at the species level, revealing four key insights. First, we discovered that Lawsonella clevelandensis, a recently named bacterium, and Neisseriaceae [G-1] HMT-174, a previously unrecognized bacterium, are common in adult nostrils. Second, just 19 species accounted for 90% of the total sequences from all participants. Third, 1 of these 19 species belonged to a currently uncultivated genus. Fourth, for 94% of the participants, 2 to 10 species constituted 90% of their sequences, indicating that the nostril microbiome may be represented by limited consortia. These insights highlight the strengths of the nostril microbiome as a model system for studying interspecies interactions and microbiome function. Also, in this cohort, three common nasal species (Dolosigranulum pigrum and two Corynebacterium species) showed positive differential abundance when the pathobiont Staphylococcus aureus was absent, generating hypotheses regarding colonization resistance. By facilitating species-level taxonomic assignment to microbes from the human aerodigestive tract, the eHOMD is a vital resource enhancing clinical relevance of microbiome studies. IMPORTANCE The eHOMD (http://www.ehomd.org) is a valuable resource for researchers, from basic to clinical, who study the microbiomes and the individual microbes in body sites in the human aerodigestive tract, which includes the nasal passages, sinuses, throat, esophagus, and mouth, and the lower respiratory tract, in health and disease. The eHOMD is an actively curated, web-based, open-access resource. eHOMD provides the following: (i) species-level taxonomy based on grouping 16S rRNA gene sequences at 98.5% identity, (ii) a systematic naming scheme for unnamed and/or uncultivated microbial taxa, (iii) reference genomes to facilitate metagenomic, metatranscriptomic, and proteomic studies and (iv) convenient cross-links to other databases (e.g., PubMed and Entrez). By facilitating the assignment of species names to sequences, the eHOMD is a vital resource for enhancing the clinical relevance of 16S rRNA gene-based microbiome studies, as well as metagenomic studies.

scholarly journals Erythrobacter spongiae sp. nov., isolated from marine sponge

2019 ◽  
Vol 69 (4) ◽  
pp. 1111-1116 ◽  
Author(s):  
Lingping Zhuang ◽  
Binbin Lin ◽  
Li Xu ◽  
Guoqiang Li ◽  
Chang-Jer Wu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Hydrolysis Of ◽  
Distinct Phylogenetic Lineage

A taxonomic study was carried out on strain HN-E23T, which was isolated from sponge collected from Yangpu Bay, Hainan, China. Cells of strain HN-E23T were Gram-stain-negative, non-motile, orange-yellow-pigmented, short rods, that could grow at 10–40 °C (optimum, 28 °C), at pH 5–11 (optimun, pH 7) and in 0.5–12 % (w/v) NaCl (optimum, 3 %). This isolate was positive for oxidase, catalase, and the hydrolysis of aesculin, but negative for indole production and the reduction of nitrate. The phylogenetic tree based on 16S rRNA gene sequences revealed that strain HN-E23T formed a distinct phylogenetic lineage within the cluster comprising Erythrobacter strains. Strain HN-E23T shared the highest 16S rRNA gene sequence similarity to Erythrobacter aquimixticola JSSK-14T (97.2 %), followed by Erythrobacter atlanticus s21-N3T (96.6 %), Erythrobacter luteus KA37T (96.5 %) and Erythrobacter citreus RE35F/1T (96.4 %). The digital DNA–DNA hybridization (dDDH) and the average nucleotide identity (ANI) values between strain HN-E23T and JSSK-14T were 18.8 and 74.9 %, respectively. The dDDH and ANI values are below the standard cut-off criteria for delineation of bacterial species. The dominant fatty acids were summed feature 8 (C18 : 1ω7c/ω6c), C16 : 0 and summed feature 3 (C16 : 1ω7c/ω6c). The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid, an unidentified glycolipid and six unidentified lipids. The respiratory lipoquinone was identified as Q-10. The G+C content of the genomic DNA was 65.5 mol%. Based on the phenotypic and phylogenetic data, strain HN-E23T represents a novel species of the genus Erythrobacter , for which the name Erythrobacter spongiae sp. nov. is proposed, with the type strain HN-E23T (=MCCC 1K03331T=LMG 30457T).

Solirubrobacter phytolaccae sp. nov., an endophytic bacterium isolated from roots of Phytolacca acinosa Roxb.

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 858-862 ◽  
Author(s):  
Linfang Wei ◽  
Shan Ouyang ◽  
Yao Wang ◽  
Xihui Shen ◽  
Lei Zhang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Bacterial Species ◽  
Shaanxi Province ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Link Type

A Gram-staining-positive, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated GTGR-8T, which formed white colonies, was isolated from roots of Phytolacca acinosa Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China. Strain GTGR-8T grew optimally at 28–30 °C, at pH 7.0–8.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTGR-8T was a member of the genus Solirubrobacter and was closely related to Solirubrobacter pauli B33D1T (98.9 % similarity), Solirubrobacter ginsenosidimutans BXN5-15T (97.0 %) and Solirubrobacter soli Gsoil 355T (96.9 %). No other recognized bacterial species showed more than 94.2 % 16S rRNA gene sequence similarity to the novel isolate. The only respiratory quinone of strain GTGR-8T was MK-7(H4) and the major fatty acids (>5 %) were iso-C16 : 0, C18 : 1ω9c, C17 : 1ω8c, C18 : 3ω6c (6,9,12) and C17 : 1ω6c. The DNA G+C content was 71.0 mol%. DNA–DNA relatedness for strain GTGR-8T with respect to its closest relatives, S. pauli KCTC 9974T and S. ginsenosidimutans KCTC 19420T, was 52.5 and 24.5 %, respectively. Based on phenotypic, phylogenetic and genotypic data, strain GTGR-8T is considered to represent a novel species in the genus Solirubrobacter , for which the name Solirubrobacter phytolaccae sp. nov. is proposed. The type strain is GTGR-8T ( = CCTCC AB 2013011T = KCTC 29190T).

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