Cloning and functional characterization of a fatty acid Δ6-desaturase from Oenothera biennis: Production of γ-linolenic acid by heterologous expression in Saccharomyces cerevisiae

2010 ◽  
Vol 57 (4) ◽  
pp. 568-573 ◽  
Author(s):  
Shenghe Huang ◽  
Ruijie Liu ◽  
Yiding Niu ◽  
Agula Hasi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Heterologous Expression ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Oenothera Biennis ◽  
Δ6 Desaturase

scholarly journals Heterologous Expression of PA8FAD9 and Functional Characterization of a Δ9-Fatty Acid Desaturase from a Cold-Tolerant Pseudomonas sp. A8

2016 ◽  
Vol 58 (11) ◽  
pp. 718-728 ◽  
Author(s):  
Lawal Garba ◽  
Mohd Shukuri Mohamad Ali ◽  
Siti Nurbaya Oslan ◽  
Raja Noor Zaliha Raja Abdul Rahman
Keyword(s):  
Fatty Acid ◽  
Heterologous Expression ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Pseudomonas Sp ◽  
Cold Tolerant

scholarly journals Heterologous expression and characterization of bacterial 2-C-methyl-d-erythritol-4-phosphate pathway in Saccharomyces cerevisiae

2013 ◽  
Vol 97 (13) ◽  
pp. 5753-5769 ◽  
Author(s):  
Simon Carlsen ◽  
Parayil Kumaran Ajikumar ◽  
Luca Riccardo Formenti ◽  
Kang Zhou ◽  
Too Heng Phon ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heterologous Expression

scholarly journals Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome

3 Biotech ◽  
2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Ravi K. Shah ◽  
Amrutlal K. Patel ◽  
Deepti M. Davla ◽  
Ishan K. Parikh ◽  
Ramalingam B. Subramanian ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Functional Characterization ◽  
Cellulolytic Enzyme ◽  
Rumen Metagenome ◽  
Buffalo Rumen

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturases ◽  
Desaturase Gene ◽  
Saccharomyces Kluyveri ◽  
Fatty Acid Desaturase Gene

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.

Effect of transplanting date on the fatty acid composition, oil content and yield of evening primrose (Oenothera biennis L.) seed

1994 ◽  
Vol 74 (1) ◽  
pp. 129-131 ◽  
Author(s):  
Robert C. Roy ◽  
Peter H. White ◽  
Alex F. More ◽  
John G. Hendel ◽  
Robert Pocs ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Key Words ◽  
Linolenic Acid ◽  
Acid Content ◽  
Oil Content ◽  
Linolenic Acid Content ◽  
Oenothera Biennis ◽  
Evening Primrose ◽  
One Year

The influence of transplanting time on the growth of evening primrose (Oenothera biennis L.) was investigated for 3 yr. Delaying transplanting reduced the yield of seed in two of these years. The amount of oil in the seed was not influenced by time of transplanting, and only in one year did the γ-linolenic acid content of the oil decrease with delays in transplanting. Key words: Evening primrose, transplanting date, oil content, γ-linolenic acid

Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharomyces cerevisiae

2002 ◽  
Vol 42 (5) ◽  
pp. 252-259 ◽  
Author(s):  
Kaoru Takegawa ◽  
Sanae Tokudomi ◽  
M. Shah Alam Bhuiyan ◽  
Mitsuaki Tabuchi ◽  
Yasuko Fujita ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heterologous Expression ◽  
Schizosaccharomyces Pombe ◽  
Carboxypeptidase Y
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