Implication of cAMP in Regulation of Hydrogen Peroxide Level in Pea Seedling Roots under Biotic Stress

2020 ◽  
Vol 67 (3) ◽  
pp. 435-442
Author(s):  
L. A. Lomovatskaya ◽  
O. V. Kuzakova ◽  
A. M. Goncharova ◽  
A. S. Romanenko
Keyword(s):  
Hydrogen Peroxide ◽  
Biotic Stress ◽  
Seedling Roots ◽  
Pea Seedling

scholarly journals The Effect of Nitrogen Fertilization and Fusarium culmorum Inoculation on the Biomarkers of Oxidative Stress in Wheat Flag Leaves

Poljoprivreda ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 15-24
Author(s):  
Magdalena Matić ◽  
◽  
Rosemary Vuković ◽  
Karolina Vrandečić ◽  
Ivna Štolfa Čamagajevac ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Biotic Stress ◽  
Flag Leaf ◽  
Fusarium Culmorum ◽  
Antioxidant Response ◽  
The Impact ◽  
Biomarkers Of Oxidative Stress

During cultivation, wheat is exposed to several abiotic and/or biotic stress conditions that may adversely impact the wheat yield and quality. The impact of abiotic stress caused by nitrogen deficiency and biotic stress caused by phytopathogenic fungus Fusarium culmorum on biomarkers of oxidative stress in the flag leaf of nine winter wheat varieties (Ficko, U-1, Galloper, BC Mandica, BC Opsesija, Ingenio, Isengrain, Felix, and Bezostaya-1) was analyzed in this study. Hydrogen peroxide concentration and lipid peroxidation level were measured as indicators of oxidative stress, while the antioxidant response was determined by measuring the concentration of phenolic compounds and activities of antioxidant enzymes. Wheat variety and nitrogen treatment had a significant effect on all examined biomarkers of oxidative stress in the flag leaf, while the impact of Fusarium treatment was less pronounced. The most significant impact on the measured stress biomarkers had a low nitrogen level, which mainly increased hydrogen peroxide concentration and lipid peroxidation level and decreased activities of antioxidant enzymes in most varieties. The obtained results were discussed and compared with the previous study in which biochemical analyzes were performed on the wheat spike. There was no significant strong correlation between flag leaf and spike response in the measured parameters, which, in addition to the variety-specific response, also indicates a tissue-specific antioxidant response.

scholarly journals Study of the Effect of Azospirillum Lectins on the Formation of Hydrogen Peroxide in Wheat Seedling Roots

2012 ◽  
Vol 12 (4) ◽  
pp. 56-63
Author(s):  
S. A. Alen’kina ◽  
◽  
K. A. Trutneva ◽  
V. А. Velikov ◽  
V. E. Nikitina ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Azospirillum Brasilense ◽  
Wheat Seedling ◽  
Oxalate Oxidase ◽  
Lectin Activity ◽  
Production Of Hydrogen ◽  
Seedling Roots ◽  
Azospirillum Brasilense Sp7 ◽  
Wheat Seedling Roots ◽  
Adaptation Processes

We show that the lectins isolated from the surface of the nitrogenfixing soil bacterium Azospirillum brasilense Sp7 and its mutant defective in lectin activity, A. brasilense Sp7.2.3., can regulate the production of hydrogen peroxide in wheat seedling roots, which is associated with the activation of superoxide dismutase, peroxidase and oxalate oxidase, as well as with the inhibition of catalase activity. We show that activation of oxalate oxidase is the most rapidly inducible pathway for the formation of hydrogen peroxide in wheat seedling roots under the effect of lectins. The obtained data indicate that the Azospirillum lectins can act as inducers of adaptation processes in wheat seedling roots.

The NADPH oxidase activity of pea seedling roots in rhizobial infection depending on abiotic and biotic factors

2010 ◽  
Vol 46 (4) ◽  
pp. 438-443 ◽  
Author(s):  
A. K. Glyan’ko ◽  
G. G. Vasil’eva ◽  
A. A. Ischenko ◽  
N. V. Mironova ◽  
A. L. Alekseenko
Keyword(s):  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Biotic Factors ◽  
Nadph Oxidase Activity ◽  
Seedling Roots ◽  
Abiotic And Biotic Factors ◽  
Pea Seedling

Proteomic analysis of the effect of methyl jasmonate on pea seedling roots

2013 ◽  
Vol 449 (1) ◽  
pp. 90-93 ◽  
Author(s):  
V. G. Yakovleva ◽  
A. M. Egorova ◽  
I. A. Tarchevsky
Keyword(s):  
Methyl Jasmonate ◽  
Proteomic Analysis ◽  
Seedling Roots ◽  
Pea Seedling

scholarly journals Hydrogen Peroxide Imbibition Following Cold Stratification Promotes Seed Germination Rate and Uniformity in Peach cv. GF305

Seeds ◽  
2022 ◽  
Vol 1 (1) ◽  
pp. 28-35
Author(s):  
Gregorio Barba-Espín ◽  
José A. Hernández ◽  
Cristina Martínez-Andújar ◽  
Pedro Díaz-Vivancos
Keyword(s):  
Hydrogen Peroxide ◽  
Abscisic Acid ◽  
Seed Germination ◽  
Practical Implication ◽  
Germination Rate ◽  
Cold Stratification ◽  
Enzymatic Antioxidants ◽  
Breeding Programs ◽  
Hormone Profile ◽  
Seedling Roots

(1) Background: Peach cv. GF305 is commonly used in breeding programs due to its susceptibility to numerous viruses. In this study, we aimed to achieve a methodology for rapid and uniform seed germination of peach cv. GF305 in order to obtain vigorous seedlings; (2) Methods: A combination of cold stratification and H2O2 imbibition was tested on peach seeds with or without endocarp. In addition, the levels of non-enzymatic antioxidants ascorbate and glutathione as well as the hormone profile in seedling roots and shoots were determined; (3) Results: We found that H2O2 imbibition of peach seeds without endocarp after 8 weeks of stratification increased germination rate and resulted in seedlings displaying good vegetative growth. The H2O2 imbibition also affected the levels of ascorbate, glutathione, and the phytohormones abscisic acid and jasmonic acid in peach seedlings; (4) Conclusions: Although stratification periods of 12 weeks have been previously established as being appropriate for this cultivar, we have been able to reduce this stratification time by up to 4 weeks, which may have practical implication in peach nurseries.

The synthesis of messenger and ribosomal RNA in pea seedlings as detected by electrophoresis

1965 ◽  
Vol 162 (986) ◽  
pp. 121-136 ◽  
Keyword(s):  
Cellulose Acetate ◽  
Ribosomal Rna ◽  
Base Composition ◽  
Cellulose Acetate Membrane ◽  
Citrate Buffer ◽  
Pea Seedlings ◽  
Seedling Roots ◽  
Membranous Component ◽  
Pea Seedling

This paper describes a method for the analysis of microsome fractions from pea seedling roots by electrophoresis on cellulose acetate membrane. The method separates the lipid membranous component, nucleoprotein and free RNA . The synthesis of RNA in the growing root has been studied by incubating the seedlings in radioactive phosphate, and analysing the isolated microsomal fractions by electrophoresis. A rapidly labelled nucleoprotein could be distinguished from the ribosomes, which remain unlabelled for nearly one hour. After longer incubation times, the ribosomes become labelled. Citrate buffer causes the dissociation of a trace of highly labelled RNA from the labelled ribosomes. It was found that the base composition of this RNA and of the RNA of the rapidly labelled nucleoprotein, differs from that of ribosomal or transfer RNA and is somewhat more like that of DNA .

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