Hydrogen Peroxide Imbibition Following Cold Stratification Promotes Seed Germination Rate and Uniformity in Peach cv. GF305

(1) Background: Peach cv. GF305 is commonly used in breeding programs due to its susceptibility to numerous viruses. In this study, we aimed to achieve a methodology for rapid and uniform seed germination of peach cv. GF305 in order to obtain vigorous seedlings; (2) Methods: A combination of cold stratification and H2O2 imbibition was tested on peach seeds with or without endocarp. In addition, the levels of non-enzymatic antioxidants ascorbate and glutathione as well as the hormone profile in seedling roots and shoots were determined; (3) Results: We found that H2O2 imbibition of peach seeds without endocarp after 8 weeks of stratification increased germination rate and resulted in seedlings displaying good vegetative growth. The H2O2 imbibition also affected the levels of ascorbate, glutathione, and the phytohormones abscisic acid and jasmonic acid in peach seedlings; (4) Conclusions: Although stratification periods of 12 weeks have been previously established as being appropriate for this cultivar, we have been able to reduce this stratification time by up to 4 weeks, which may have practical implication in peach nurseries.

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Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽

10.3390/horticulturae7110490 ◽

2021 ◽

Vol 7 (11) ◽

pp. 490

Author(s):

Saeng Geul Baek ◽

Jin Hyun Im ◽

Myeong Ja Kwak ◽

Cho Hee Park ◽

Mi Hyun Lee ◽

...

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Germination Rate ◽

Seed Viability ◽

Cold Stratification ◽

Dormancy Breaking ◽

Viability Test ◽

Physiological Dormancy ◽

Different Temperatures ◽

Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

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Pre-Sowing Treatments Improve Germinability of South Texas Native Plant Seeds

Plants ◽

10.3390/plants10112545 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2545

Author(s):

Kaitlynn Lavallee ◽

Pushpa Gautam Soti ◽

Hansapani Rodrigo ◽

Rupesh Kariyat ◽

Alexis Racelis

Keyword(s):

Seed Germination ◽

Plant Species ◽

Native Plants ◽

Germination Rate ◽

South Texas ◽

Cold Stratification ◽

Native Plant ◽

Native Plant Species ◽

Plant Seeds ◽

Wide Range

The incorporation of native plant species is central to restoration efforts, but this is often limited by both the availability of seeds and the relatively low viability and germination rates of commercially available seeds. Although pre-sowing treatments are commonly used to improve germination rates of seeds, the efficacy of these treatments is found to vary across species. In this study, we tested how four pre-sow treatments (physical scarification, acid scarification, cold stratification, and aerated hydropriming) affected the viability and seed germination rates of 12 commercially available plant species native to south Texas and commonly used in restoration efforts. Our results show that the viability of the seeds have a wide range, from 78% to 1.25%. Similarly, the total germination rate ranged from 62% to 0%. We found that pre-sowing treatments accelerated the germination rate in 9 of 12 plant species tested, but the effect varied by treatment. Collectively, our results identify various methods to achieve the best germination rates for native plants of south Texas, to help improve restoration efforts across the region.

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Influence of an abscisic acid (ABA) seed coating on seed germination rate and timing of Bluebunch Wheatgrass

Ecology and Evolution ◽

10.1002/ece3.5212 ◽

2019 ◽

Vol 9 (13) ◽

pp. 7438-7447 ◽

Cited By ~ 3

Author(s):

William C. Richardson ◽

Turmandakh Badrakh ◽

Bruce A. Roundy ◽

Zackary T. Aanderud ◽

Steven L. Petersen ◽

...

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Germination Rate ◽

Seed Coating ◽

Bluebunch Wheatgrass

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Effects of partial drying on seed germination in the aquatic grasses Zizania palustris L. and Porteresia coarctata (Roxb.) Tateoka

Seed Science Research ◽

10.1017/s0960258500001380 ◽

1992 ◽

Vol 2 (4) ◽

pp. 199-205 ◽

Cited By ~ 8

Author(s):

C. D. Aldridge ◽

R. J. Probert

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Growth Regulators ◽

Germination Percentage ◽

Hormonal Status ◽

Cold Stratification ◽

Porteresia Coarctata ◽

Germination Response ◽

Fold Reduction ◽

Germination Behaviour

AbstractPartial drying of non-dormant seeds had little effect on germination behaviour compared with undried controls. In contrast, partial drying resulted in a marked increase in the germination response of freshly harvested (dormant) seeds of Z. palustris. Partial drying also resulted in a 100-fold reduction in the concentration of gibberellins (GA4+7) required for maximum germination. Although the concentration range of applied abscisic acid (ABA) that permitted germination was unaltered, partial drying increased the final germination percentage at all concentrations tested. The concentration of ABA above which germination was reduced in freshly harvested (dormant) seeds of Z. palustris was 3.16 × 10−7m compared with 10−4m in fresh (non-dormant) seeds of P. coarctata. Changes in the germination response of Z. palustris seeds to applied growth regulators, following partial drying or cold stratification might be explained by similar changes in hormonal status.

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EFFECTS OF COLD STRATIFICATION AND HORMONES ON SEED GERMINATION OF SARRACENIA ALATA

Texas Journal of Science ◽

10.32011/txjsci_71_1_article7 ◽

2019 ◽

Vol 71 (1) ◽

Author(s):

K.A. Hopkins ◽

D.A. Gravatt

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Cold Stratification ◽

Percent Germination ◽

Germination Requirements

Abstract Seed germination requirements for S. alata were investigated using cold stratification and phytohormones. Treatments included no stratification (control), two week, three week, four week, five week, and six week cold stratification. Hormone treatments included no stratification with cytokinin, no stratification with gibberellins, no stratification with cytokinin and gibberellins, no stratification with auxin, and one with six week stratification with abscisic acid. Three weeks cold stratification was required to yield a significant increase in percent germination. Gibberellin was the only hormone which caused a significant increase in germination in S. alata seeds.

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Studies on the hawthorn (Crataegus azarollusL.): II. Changes in abscisic acid content during cold stratification in relation to seed germination

Journal of Horticultural Science ◽

10.1080/00221589.1991.11516148 ◽

1991 ◽

Vol 66 (2) ◽

pp. 223-226 ◽

Cited By ~ 3

Author(s):

M. M. Qrunfleh

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Acid Content ◽

Cold Stratification ◽

Abscisic Acid Content

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Effects of cold stratification on the endogenous hormone, dormancy and germination of Cornus walteri Wanger seeds

Bangladesh Journal of Botany ◽

10.3329/bjb.v49i3.49617 ◽

2020 ◽

Vol 49 (3) ◽

pp. 507-514

Author(s):

Li Donglin ◽

Jin Yaquin ◽

Yu Chengjing ◽

Xue Yuan

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Environmental Factors ◽

Seed Dormancy ◽

Zeatin Riboside ◽

Endogenous Hormone ◽

Gas Content ◽

Cold Stratification ◽

Plant Life ◽

Critical Phase

The most critical phase in plant life is the germination period, which is influenced by both intrinsic and environmental factors. Assessment of cold stratification on several endogenous hormone, IAA, abscisic acid (ABA), gibberellin GA1/3 (GA1/3), zeatin-riboside (ZRs) and isopen-tenyl adenine (iPAs), and germination of Cornus walteri Wanger. seeds was done. Relationship between endogenous hormone and seed germination and mechanism of seed dormancy of C. walteri were also analysed. The results showed that the significant fluctuatation of both IAA and iPAs content was fond during cold stratification period, while the variation of ZRs was little, that of both ABA and GAs content increased with old stratification days. Effects of cold stratification on both GR and GP were significant (p < 0.05), which play an important role in releaving of seed germination and improving seed germination. The GR and GP were significantly negatively correlated with the contents of ABA and GA1/3, and positively correlated with the following iPAs, ZRs/ABA, iPAs/ABA.

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IMPROVEMENT IN SEED GERMINATION IN PURPLE CONEFLOWER (ECHINACEA PURPUREA) AFTER COLD STRATIFICATION OR OSMOTIC PRIMING.

HortScience ◽

10.21273/hortsci.25.9.1088c ◽

1990 ◽

Vol 25 (9) ◽

pp. 1088c-1088

Author(s):

N. Wartidiningsih ◽

Robert L. Geneve

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Field Tests ◽

Echinacea Purpurea ◽

Cold Stratification ◽

Stage Of Development ◽

Purple Coneflower ◽

Physiological Dormancy ◽

Wide Variability ◽

Percent Germination

Germination was evaluated in six seed lots of purple coneflower purchased from four different seed companies. Standard germination percent ranged from 28% to 90% depending on the seed lot. For seed collected in 1989, seed size and stage of development of the seed at harvest could not account for the wide variability in seed germination observed in the purchased seed lots. preconditioning the seed with either cold stratification (10°C for 10 days) or osmotic priming (PEG or salt solution at -5 bars for 5 days) increased the rate of germination and the overall percent germination for all seed lots and dramatically improved germination in the poor germinating seed lots. Preconditioning appears to overcome either a shallow physiological dormancy or compensates for seeds with poor vigor or quality. In either case, seed preconditioning drastically improved seed germination (rate and percent) in greenhouse and field tests for purple coneflower.

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