Functions of transcription factor TRF2 Drosophila melanogaster

We previously showed in Drosophila melanogaster embryos that low-affinity Ultrabithorax (Ubx)-responsive shavenbaby (svb) enhancers drive expression using localized transcriptional environments and that active svb enhancers on different chromosomes tended to colocalize (Tsai et al., 2017). Here, we test the hypothesis that these multi-enhancer ‘hubs’ improve phenotypic resilience to stress by buffering against decreases in transcription factor concentrations and transcriptional output. Deleting a redundant enhancer from the svb locus led to reduced trichome numbers in embryos raised at elevated temperatures. Using high-resolution fluorescence microscopy, we observed lower Ubx concentration and transcriptional output in this deletion allele. Transcription sites of the full svb cis-regulatory region inserted into a different chromosome colocalized with the svb locus, increasing Ubx concentration, the transcriptional output of svb, and partially rescuing the phenotype. Thus, multiple enhancers could reinforce a local transcriptional hub to buffer against environmental stresses and genetic perturbations, providing a mechanism for phenotypical robustness.

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Role of FOXO transcription factor in radiation adaptive response and hormesis in Drosophila melanogaster

BIOPHYSICS ◽

10.1134/s0006350910050301 ◽

2010 ◽

Vol 55 (5) ◽

pp. 854-858

Author(s):

M. V. Shaposhnikov ◽

A. A. Moskalev

Keyword(s):

Transcription Factor ◽

Drosophila Melanogaster ◽

Adaptive Response ◽

Foxo Transcription Factor

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The Short antennae gene of Tribolium is required for limb development and encodes the orthologue of the Drosophila Distal-less protein

Development ◽

10.1242/dev.128.2.287 ◽

2001 ◽

Vol 128 (2) ◽

pp. 287-297 ◽

Cited By ~ 3

Author(s):

A. Beermann ◽

D.G. Jay ◽

R.W. Beeman ◽

M. Hulskamp ◽

D. Tautz ◽

...

Keyword(s):

Transcription Factor ◽

Drosophila Melanogaster ◽

Genetic Mapping ◽

Tribolium Castaneum ◽

Limb Development ◽

Distal Region ◽

Distal Limb ◽

Functional Conservation ◽

Young Embryo ◽

Evolutionary Changes

Insects bear a stereotyped set of limbs, or ventral body appendages. In the highly derived dipteran Drosophila melanogaster, the homeodomain transcription factor encoded by the Distal-less (Dll) gene plays a major role in establishing distal limb structures. We have isolated the Dll orthologue (TcDll) from the beetle Tribolium castaneum, which, unlike Drosophila, develops well-formed limbs during embryogenesis. TcDll is initially expressed at the sites of limb primordia formation in the young embryo and subsequently in the distal region of developing legs, antennae and mouthparts except the mandibles. Mutations in the Short antennae (Sa) gene of Tribolium delete distal limb structures, closely resembling the Dll phenotype in Drosophila. TcDll expression is severely reduced or absent in strong Sa alleles. Genetic mapping and molecular analysis of Sa alleles also support the conclusion that TcDll corresponds to the Sa gene. Our data indicate functional conservation of the Dll gene in evolutionarily distant insect species. Implications for evolutionary changes in limb development are discussed.

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Drosophila Zic family member odd-paired is needed for adult post-ecdysis maturation

Open Biology ◽

10.1098/rsob.190245 ◽

2019 ◽

Vol 9 (12) ◽

pp. 190245

Author(s):

Eléanor Simon ◽

Sergio Fernández de la Puebla ◽

Isabel Guerrero

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Transcription Factor ◽

Drosophila Melanogaster ◽

Family Member ◽

Ectopic Expression ◽

Functional Conservation ◽

The Central Nervous System ◽

Behavioural Sequence

Specific neuropeptides regulate in arthropods the shedding of the old cuticle (ecdysis) followed by maturation of the new cuticle. In Drosophila melanogaster , the last ecdysis occurs at eclosion from the pupal case, with a post-eclosion behavioural sequence that leads to wing extension, cuticle stretching and tanning. These events are highly stereotyped and are controlled by a subset of crustacean cardioactive peptide (CCAP) neurons through the expression of the neuropeptide Bursicon (Burs). We have studied the role of the transcription factor Odd-paired (Opa) during the post-eclosion period. We report that opa is expressed in the CCAP neurons of the central nervous system during various steps of the ecdysis process and in peripheral CCAP neurons innerving the larval muscles involved in adult ecdysis. We show that its downregulation alters Burs expression in the CCAP neurons. Ectopic expression of Opa, or the vertebrate homologue Zic2 , in the CCAP neurons also affects Burs expression, indicating an evolutionary functional conservation. Finally, our results show that, independently of its role in Burs regulation, Opa prevents death of CCAP neurons during larval development.

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Assembly of SNAPc, Bdp1, and TBP on the U6 snRNA Gene Promoter in Drosophila melanogaster

Molecular and Cellular Biology ◽

10.1128/mcb.00641-19 ◽

2020 ◽

Vol 40 (12) ◽

Author(s):

Mun Kyoung Kim ◽

An Tranvo ◽

Ann Marie Hurlburt ◽

Neha Verma ◽

Phuc Phan ◽

...

Keyword(s):

Transcription Factor ◽

Drosophila Melanogaster ◽

Rna Polymerase Iii ◽

Gene Promoter ◽

Stable Complex ◽

Sequence Element ◽

Content Type ◽

U6 Snrna ◽

U6 Promoter ◽

Pol Iii

ABSTRACT U6 snRNA is transcribed by RNA polymerase III (Pol III) and has an external upstream promoter that consists of a TATA sequence recognized by the TBP subunit of the Pol III basal transcription factor IIIB and a proximal sequence element (PSE) recognized by the small nuclear RNA activating protein complex (SNAPc). Previously, we found that Drosophila melanogaster SNAPc (DmSNAPc) bound to the U6 PSE can recruit the Pol III general transcription factor Bdp1 to form a stable complex with the DNA. Here, we show that DmSNAPc-Bdp1 can recruit TBP to the U6 promoter, and we identify a region of Bdp1 that is sufficient for TBP recruitment. Moreover, we find that this same region of Bdp1 cross-links to nucleotides within the U6 PSE at positions that also cross-link to DmSNAPc. Finally, cross-linking mass spectrometry reveals likely interactions of specific DmSNAPc subunits with Bdp1 and TBP. These data, together with previous findings, have allowed us to build a more comprehensive model of the DmSNAPc-Bdp1-TBP complex on the U6 promoter that includes nearly all of DmSNAPc, a portion of Bdp1, and the conserved region of TBP.

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