Induction of Proline Iminopeptidase by Nitric Oxide May Result in the Proline Accumulation in the Pea Roots

Nitric oxide (NO) – an endogenous signalling molecule in plants and animals – mediates responses to biotic and abiotic stresses. In the present study, we examined the role of exogenous application of NO in mediating stress responses in Cakile maritima Scop. seedlings under water deficit stress using sodium nitroprusside (SNP) as NO donor and as a pre-treatment before the application of stress. Water deficit stress was applied by withholding water for 14 days. Growth, leaf water content (LWC), osmotic potential (ψs), chlorophyll, malondialdehyde (MDA), electrolyte leakage (EL), proline and Δ1-pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) protein levels were determined. Enzyme activities involved in antioxidant activities (superoxide dismutase (SOD) and catalase (CAT)) were measured upon withholding water. The results showed that shoot biomass production was significantly decreased in plants subjected to water deficit stress alone. However, in water deficit stressed plants pre-treated with SNP, growth activity was improved and proline accumulation was significantly increased. Proline accumulation was concomitant with the stimulation of its biosynthesis as shown by the accumulation of P5CS proteins. Nevertheless, no significant change in ProDH protein levels was observed. Besides plants showed lower water deficit-induced lipid membrane degradation and oxidative stress after the pretreatment with 100 µM SNP. This behaviour was related to the increased activity of SOD and CAT. Thus, we concluded that NO increased C. maritima drought tolerance and mitigated damage associated with water deficit stress by the regulation of proline metabolism and the reduction of oxidative damage.

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Pretreatment with nitric oxide reduces lead toxicity in cowpea (Vigna unguiculata [L.] walp.)

Archives of Biological Sciences ◽

10.2298/abs150325139s ◽

2016 ◽

Vol 68 (1) ◽

pp. 165-175 ◽

Cited By ~ 10

Author(s):

Omid Sadeghipour

Keyword(s):

Nitric Oxide ◽

Lipid Peroxidation ◽

Stress Tolerance ◽

Net Photosynthetic Rate ◽

Photosynthetic Rate ◽

Proline Accumulation ◽

Antioxidant Enzyme Activities ◽

Toxic Heavy Metals ◽

Pb Toxicity ◽

Pb Stress

Lead (Pb) is one of the most important toxic heavy metals that reduce plant growth and development. Therefore, finding compounds that can alleviate the toxic effects of Pb is necessary. Nitric oxide (NO) is a signaling molecule that plays important roles in different physiological processes in plants, such as the response to biotic and abiotic stresses. A pot experiment was conducted to study the effects of exogenously applied NO on Pb stress tolerance in cowpea. Seeds were soaked at various concentrations of sodium nitroprusside (SNP; NO donor) (0, 0.5 and 1 mM) for 20 h, and then sown in pots containing 0 or 200 mg kg-1 Pb (NO3)2. The obtained results showed that Pb stress significantly reduced the chlorophyll value, relative water content (RWC) and net photosynthetic rate, but increased lipid peroxidation, proline content and superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) activities. Nonetheless, different levels of NO significantly lowered Pb toxicity by further increasing SOD, CAT, APX and GR activities as well as proline accumulation. NO pretreatment also raised the chlorophyll value, RWC and net photosynthetic rate but reduced lipid peroxidation. Both NO levels (0.5 and 1 mM) were effective in Pb stress tolerance; however, 0.5 mM was more effective. These results indicate that NO pretreatment plays an important role in protecting cowpea plants against Pb toxicity by enhancing antioxidant enzyme activities and proline accumulation.

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Hydrogen-induced osmotic tolerance is associated with nitric oxide-mediated proline accumulation and reestablishment of redox balance in alfalfa seedlings

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2017.12.022 ◽

2018 ◽

Vol 147 ◽

pp. 249-260 ◽

Cited By ~ 24

Author(s):

Jiuchang Su ◽

Yihua Zhang ◽

Yang Nie ◽

Dan Cheng ◽

Ren Wang ◽

...

Keyword(s):

Nitric Oxide ◽

Redox Balance ◽

Proline Accumulation ◽

Osmotic Tolerance

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Effect of nodule and pathogenic bacteria on levels of nitric oxide and cyclic adenosine monophosphate in pea roots at initial stages of interaction

Proceedings of universities Applied chemistry and biotechnology ◽

10.21285/2227-2925-2020-10-2-294-302 ◽

2020 ◽

Vol 10 (2) ◽

pp. 294-302

Author(s):

A. A. Ishchenko ◽

N. V. Filinova ◽

A. V. Sidorov

Keyword(s):

Nitric Oxide ◽

Pathogenic Bacteria ◽

Cyclic Adenosine Monophosphate ◽

Adenosine Monophosphate ◽

Pea Roots

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Abscisic acid-induced nitric oxide and proline accumulation in independent pathways under water-deficit stress during seedling establishment in Medicago truncatula

Journal of Experimental Botany ◽

10.1093/jxb/eru088 ◽

2014 ◽

Vol 65 (8) ◽

pp. 2161-2170 ◽

Cited By ~ 38

Author(s):

Elisabeth Planchet ◽

Isabelle Verdu ◽

Julien Delahaie ◽

Caroline Cukier ◽

Clément Girard ◽

...

Keyword(s):

Nitric Oxide ◽

Abscisic Acid ◽

Water Deficit ◽

Medicago Truncatula ◽

Seedling Establishment ◽

Proline Accumulation ◽

Water Deficit Stress

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Localization of endothelial nitric oxide synthase in the normal and failing human atrial myocardium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166397 ◽

1996 ◽

Vol 54 ◽

pp. 786-787

Author(s):

Chi-Ming Wei ◽

Margarita Bracamonte ◽

Shi-Wen Jiang ◽

Richard C. Daly ◽

Christopher G.A. McGregor ◽

...

Keyword(s):

Nitric Oxide ◽

Heart Failure ◽

Nitric Oxide Synthase ◽

Endothelial Nitric Oxide Synthase ◽

Cardiac Tissues ◽

Mammalian Tissues ◽

End Stage Heart Failure ◽

End Stage ◽

Oxide Synthase ◽

Endothelial Nitric Oxide

Nitric oxide (NO) is a potent endothelium-derived relaxing factor which also may modulate cardiomyocyte inotropism and growth via increasing cGMP. While endothelial nitric oxide synthase (eNOS) isoforms have been detected in non-human mammalian tissues, expression and localization of eNOS in the normal and failing human myocardium are poorly defined. Therefore, the present study was designed to investigate eNOS in human cardiac tissues in the presence and absence of congestive heart failure (CHF).Normal and failing atrial tissue were obtained from six cardiac donors and six end-stage heart failure patients undergoing primary cardiac transplantation. ENOS protein expression and localization was investigated utilizing Western blot analysis and immunohistochemical staining with the polyclonal rabbit antibody to eNOS (Transduction Laboratories, Lexington, Kentucky).

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