Drought-induced proline accumulation is uninvolved with increased nitric oxide, which alleviates drought stress by decreasing transpiration in rice

The purpose of the study was to determine the relationship between the messenger molecule Nitric oxide (NO) and antioxidative enzyme (SOD: Superoxide Dismutase; CAT: Catalase; APX: Ascorbate Peroxidase) activities in some metabolic changes that occur under the effect of drought stress in plants, to determine the possible roles of Nitric Oxide and to obtain complementary information. The experiment conducted in a controlled environment, and plant were cultured in containers containing Hoagland nutrient solution. For drought stress application, 10% Polyethylene Glycol (PEG 6000) was added to the nutrient solution, which is equivalent to -0.40 MPa osmotic potential. Before the drought stress is applied, pepper seedlings of Demre cv were pre-treated with different doses of Sodium Nitroprusside (SNP) and Carboxy-PTIO (potassium salt) (cPTIO) (SNP 0.01, SNP 1, SNP 100 and SNP 0.01 + cPTIO, SNP + cPTIO, SNP 100+ cPTIO). On the 10th day of the drought application, the growth parameters of the plants; the plant fresh weights and their Antioxidative Enzyme Activities (SOD, CAT, APX) were determined. In terms of plant growth parameters, both plant growth and antioxidant anzyme activities of plants pretreated with 0.01 and 1 doses of SNP were lower than the high dose of SNP and the PEG application without pretreatment. The reason for the low enzyme activities in these applications can be attributed to factors such as the excess accumulation of organic acids such as proline in the cells of the plants and the decrease in H2O2 and O-2 levels in the presence of SNP.

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Production of Nitric Oxide and Phosphatidic Acid is Involved in Activation of Plasma Membrane H+-ATPase in Maize Root Tips in Simulated Drought Stress

Russian Journal of Plant Physiology ◽

10.1134/s1021443719010035 ◽

2019 ◽

Vol 66 (1) ◽

pp. 50-58

Author(s):

Z. F. An ◽

F. J. Liu ◽

D. Y. Feng ◽

Q. L. Xu ◽

C. J. Zhou

Keyword(s):

Nitric Oxide ◽

Plasma Membrane ◽

Drought Stress ◽

Phosphatidic Acid ◽

Maize Root ◽

Root Tips ◽

Simulated Drought

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Role of nitric oxide in drought stress

10.1016/b978-0-12-818797-5.00030-3 ◽

2022 ◽

pp. 197-210

Author(s):

Abid Ullah ◽

Mohammad Ihsan ◽

Muhammad Laiq ◽

Mohammad Nisar ◽

Ali Hazrat ◽

...

Keyword(s):

Nitric Oxide ◽

Drought Stress

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Identification of Exogenous Nitric Oxide-Responsive miRNAs from Alfalfa (Medicago sativa L.) under Drought Stress by High-Throughput Sequencing

Genes ◽

10.3390/genes11010030 ◽

2019 ◽

Vol 11 (1) ◽

pp. 30

Author(s):

Yaodong Zhao ◽

Wenjing Ma ◽

Xiaohong Wei ◽

Yu Long ◽

Ying Zhao ◽

...

Keyword(s):

Nitric Oxide ◽

Drought Stress ◽

Medicago Sativa ◽

High Throughput ◽

Drought Resistance ◽

High Throughput Sequencing ◽

Target Genes ◽

Expression Profiles ◽

Differentially Expressed ◽

Medicago Sativa L

Alfalfa (Medicago sativa L.) is a high quality leguminous forage. Drought stress is one of the main factors that restrict the development of the alfalfa industry. High-throughput sequencing was used to analyze the microRNA (miRNA) profiles of alfalfa plants treated with CK (normal water), PEG (polyethylene glycol-6000; drought stress), and PEG + SNP (sodium nitroprusside; nitric oxide (NO) sprayed externally under drought stress). We identified 90 known miRNAs belonging to 46 families and predicted 177 new miRNAs. Real-time quantitative fluorescent PCR (qRT-PCR) was used to validate high-throughput expression analysis data. A total of 32 (14 known miRNAs and 18 new miRNAs) and 55 (24 known miRNAs and 31 new miRNAs) differentially expressed miRNAs were identified in PEG and PEG + SNP samples. This suggested that exogenous NO can induce more new miRNAs. The differentially expressed miRNA maturation sequences in the two treatment groups were targeted by 86 and 157 potential target genes, separately. The function of target genes was annotated by gene ontology (GO) enrichment and kyoto encyclopedia of genes and genomes (KEGG) analysis. The expression profiles of nine selected miRNAs and their target genes verified that their expression patterns were opposite. This study has documented that analysis of miRNA under PEG and PEG + SNP conditions provides important insights into the improvement of drought resistance of alfalfa by exogenous NO at the molecular level. This has important scientific value and practical significance for the improvement of plant drought resistance by exogenous NO.

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Heme Oxygenase-1 Delays Gibberellin-Induced Programmed Cell Death of Rice Aleurone Layers Subjected to Drought Stress by Interacting with Nitric Oxide

Frontiers in Plant Science ◽

10.3389/fpls.2015.01267 ◽

2016 ◽

Vol 6 ◽

Cited By ~ 7

Author(s):

Huangming Wu ◽

Yan Zheng ◽

Jing Liu ◽

Heting Zhang ◽

Huiping Chen

Keyword(s):

Nitric Oxide ◽

Cell Death ◽

Drought Stress ◽

Programmed Cell Death ◽

Heme Oxygenase ◽

Heme Oxygenase 1

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In vitro screening of rice genotypes using polyethylene glycol under drought stress

Progressive Agriculture ◽

10.3329/pa.v27i2.29321 ◽

2016 ◽

Vol 27 (2) ◽

pp. 128-135 ◽

Cited By ~ 1

Author(s):

J Akte ◽

S Yasmin ◽

MJH Bhuiyan ◽

F Khatun ◽

J Roy ◽

...

Keyword(s):

Water Stress ◽

Drought Stress ◽

Water Content ◽

Relative Water Content ◽

Dry Weight ◽

Proline Accumulation ◽

Shoot Length ◽

Fresh Weight ◽

Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

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Impacts of drought stress on growth, protein, proline, pigment content and antioxidant enzyme activities in rice (Oryza sativa L. var. BRRI dhan-24)

Dhaka University Journal of Biological Sciences ◽

10.3329/dujbs.v29i1.46537 ◽

2020 ◽

Vol 29 (1) ◽

pp. 117-123 ◽

Cited By ~ 1

Author(s):

Shamima Nasrin ◽

Shukanta Saha ◽

Hasna Hena Begum ◽

Rifat Samad

Keyword(s):

Antioxidant Enzymes ◽

Drought Stress ◽

Oryza Sativa L ◽

Proline Accumulation ◽

The Other ◽

Antioxidant Enzyme Activities ◽

Total Chlorophyll ◽

Enzymes Activities ◽

Pigment Contents ◽

Antioxidant Enzymes Activities

One of the major problems associated with rice cultivation and production is the deficiency of water resources. In here the effect of drought stress on growth, protein-, proline-, pigment contents and antioxidant enzymes activities in rice var. BRRI Dhan-24 was determined and analyzed under drought and wellwatered condition. Drought stress caused the decrease of growth and pigment contents - chlorophyll-a, b, a/b, total chlorophyll and carotenoids content of leaves of rice plants. On the other hand, the accumulation of protein, proline contents and antioxidant enzymes activities was increased under drought stress. It may be suggested that antioxidant enzymes activities and proline accumulation were associated with the growth of the plant and consequently with the mechanisms of drought tolerance in rice. Dhaka Univ. J. Biol. Sci. 29(1): 117-123, 2020 (January)

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