Sorption concentration of phenylcarboxylic acids (biomarkers of sepsis) from standard test solutions on hyper-cross-linked polystyrene

2014 ◽  
Vol 50 (6) ◽  
pp. 768-772 ◽  
Author(s):  
A. K. Pautova ◽  
A. I. Revelsky
Keyword(s):  
Standard Test ◽  
Sorption Concentration ◽  
Phenylcarboxylic Acids

Comparison of Two Tests to Determine the Maximal Aerobic Speed

2020 ◽  
Vol 60 (2) ◽  
pp. 252-262
Author(s):  
Benhammou Saddek ◽  
Jérémy B.J. Coquart ◽  
Laurent Mourot ◽  
Belkadi Adel ◽  
Mokkedes Moulay Idriss ◽  
...  
Keyword(s):  
Physiological Responses ◽  
Intraclass Correlation ◽  
Lactate Concentration ◽  
Blood Lactate Concentration ◽  
Random Order ◽  
Standard Test ◽  
Maximal Heart Rate ◽  
Distance Runners ◽  
Gold Standard Test ◽  
Maximal Aerobic Speed

SummaryThe aims of this study were (a): to compare maximal physiological responses (maximal heart rate: HRmax and blood lactate concentration: [La-]) and maximal aerobic speed (MAS) achieved during a gold standard test (T-VAM) to those during a new test entitled: the 150-50 Intermittent Test (150-50IT), and (b): to test the reliability of the 150-50IT. Eighteen middle-distance runners performed, in a random order, the T-VAM and the 150-50IT. Moreover, the runners performed a second 150-50IT (retest). The results of this study showed that the MAS obtained during 150-50IT were significantly higher than the MAS during the T-VAM (19.1 ± 0.9 vs. 17.9 ± 0.9 km.h−1, p < 0.001). There was also significant higher values in HRmax (193 ± 4 vs. 191 ± 2 bpm, p = 0.011), [La-] (11.4 ± 0.4 vs. 11.0 ± 0.5 mmol.L−1, p = 0.039) during the 150-50IT. Nevertheless, significant correlations were noted for MAS (r = 0.71, p = 0.001) and HRmax (r = 0.63, p = 0.007). MAS obtained during the first 150-50IT and the retest were not significantly different (p = 0.76) and were significantly correlated (r = 0.94, p < 0.001, intraclass correlation coefficient = 0.93 and coefficient of variation = 6.8 %). In conclusion, the 150-50IT is highly reproducible, but the maximal physiological responses derived from both tests cannot be interchangeable in the design of training programs.

Acute and chronic effect of cyaniade on survival rate, behavior, and reproduction of Daphnia sp.

2013 ◽  
Vol 1 (1) ◽  
pp. 52 ◽  
Author(s):  
Melky R Pattiwael ◽  
Remy E. P Mangindaan ◽  
Rudi Prabowo ◽  
Inneke F. M Rumengan
Keyword(s):  
Government Regulation ◽  
Aquatic Organisms ◽  
Standard Test ◽  
Toxicity Tests ◽  
Zooplankton Organism ◽  
Rate Behavior ◽  
Negative Impacts ◽  
Reproduction Pattern ◽  
Acute And Chronic Effects ◽  
Ministerial Decree

Cyanide (CN) through leaching process in the gold processing could produce tailings that have negative impacts on the aquatic environment. To determine the acute and chronic effects of cyanide on aquatic organisms, toxicity tests have been conducted in the laboratory using Daphnia sp.This zooplankton organism is recommended by APHA as standard test animals. In this study the test concentrations refer to the Ministerial Decree No. 202 of 2004 and Government Regulation No. 82 of 2001. Cyanide was analyzed as free CN and WADS CN. The result of acute toxicity test using cyanide solution showed that Daphnia could survive a maximum concentration of 0.2 ppm. LC50 values for 24 and 72 hours were 0,59 mg/L and 0,10 mg/l, respectively. The LT50 was found at 42 hours. Daphnia sp. produced different numbers of offspring at different CN concentrations, and changed their reproduction pattern from parthenogenesis to sexual reproduction after exposure to a cyanide concentration of 0.1 ppm for 24 hours©  Sianida (CN) melalui proses leaching dalam proses pengolahan emas menghasilkan limbah yang dapat memberi dampak negatif bagi lingkungan perairan. Untuk mengetahui sampai seberapa jauh CN dapat memberi efek akut dan kronis terhadap biota air, telah dilakukan uji toksisitas di laboratorium dengan menggunakan Daphnia sp. Zooplankton ini direkomendasikan oleh APHA sebagai salah satu hewan uji standar. Dalam penelitian ini konsentrasi uji yang dipakai mengacu pada peraturan yang berlaku, yaitu Keputusan Menteri No. 202, Tahun 2004, dan Peraturan Pemerintah No. 82, Tahun 2001. Analisis sianida yang dihitung adalah nilai free CN dan WADS CN. Hasil penelitian uji toksisitas akut dengan menggunakan larutan sianida didapati Daphnia mampu bertahan hidup sampai pada konsentrasi 0,2 ppm dan nilai LC50 berada pada konsentarsi 0,1 ppm serta LT50 pada jam ke 42. Hasil uji efek kronis, Daphnia sp. menghasilkan jumlah anakan yang berbeda pada konsentrasi CN yang berlainan, dan mengalami perubahan pola reproduksi dari partenogenesis menjadi seksual setelah dipaparkan pada kosentrasi sianida 0,1 ppm selama 24 jam©

Seroprevalence of leptospirosis in Chennai city, India.

2016 ◽  
Vol 5 (03) ◽  
pp. 4897 ◽  
Author(s):  
Padma Kumari J.* ◽  
Sheila Doris T. ◽  
Usha Krishnan K.
Keyword(s):  
Control Strategies ◽  
Signs And Symptoms ◽  
Laboratory Diagnosis ◽  
Community Change ◽  
Standard Test ◽  
Agglutination Test ◽  
Source Of Infection ◽  
Potential Reservoir ◽  
Study Population ◽  
Chennai City

Leptospirosis is a zoonotic disease, it occurs worldwide but is most common in tropical and subtropical countries. The magnitude of the problem varies from community to community. The serovars which cause the disease in a community change with adaptation to a new maintenance host and is epidemiologically important. Aims and Objectives: To determine the seroprevalence of leptospirosis in Chennai city. To identify the prevalent serovar in Chennai causing leptospirosis by using gold standard test Microscopic Agglutination Test (MAT). Methods: 1209 patients from Chennai, with fever of one-week duration and with signs and symptoms of Leptospirosis were the study population and were screened for Leptospirosis by Macroscopic Slide Agglutination Test (MSAT). The positive samples by MSAT were tested for the prevalent serovar and for confirmation by MAT and the results were analyzed statistically. Result: The seroprevalence of Leptospirosis is 17.8%. The prevalent serovar circulating in Chennai is L. grippotyphosa. Conclusion: Laboratory diagnosis is essential to confirm the current illness, and for epidemiological and public health reasons, namely to determine which serovar caused the infection, the likely source of infection and the potential reservoir and its location. This helps in control strategies.

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