Preconcentration of phenmedipham and desmedipham by sorption on Separcol SI C18 T for their determination in drinking water by high performance liquid chromatography

1990 ◽  
Vol 55 (9) ◽  
pp. 2146-2151 ◽  
Author(s):  
Věra Tatarkovičová
Keyword(s):  
Drinking Water ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Water Samples ◽  
High Performance ◽  
Solid Sorbent ◽  
Rp Hplc ◽  
Optimized Conditions ◽  
Drinking Water Samples

Solvent extraction used routinely for the preconcentration of carbamate herbicides from drinking water was replaced by sorption on the solid sorbent Separcol SI C18 T. Various factors of the preconcentration step that can affect the results of analysis were examined. Real drinking water samples contaminated by phenmedipham were analyzed by RP-HPLC in optimized conditions.

SELECTION OF COLUMN AND OPERATING CONDITIONS FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF PROTEINS IN CANADIAN WHEAT

1985 ◽  
Vol 65 (2) ◽  
pp. 285-298 ◽  
Author(s):  
J. E. KRUGER ◽  
B. A. MARCHYLO
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Storage Proteins ◽  
Sodium Dodecyl ◽  
Reversed Phase ◽  
Operating Conditions ◽  
Protein Patterns ◽  
Optimal Separation ◽  
Rp Hplc

Chromatographic conditions were optimized and three commercially available columns were evaluated for separation of alcohol-soluble storage proteins of Neepawa wheat using reversed-phase high-performance liquid chromatography (RP-HPLC). Optimal separation was achieved using an extracting solution of 50% 1-propanol, 1% acetic acid, and 4% dithiothreitol and an HPLC elution time of 105 min at a flow rate of 1.0 mL/min. HPLC columns evaluated (SynChropak RP-P, Ultrapore RPSC and Aquapore RP-300) varied in selectivity and resolution. The column providing the greatest versatility was Aquapore RP-300 available in cartridge form. Sodium dodecyl sulfate gradient-gel electrophoresis analysis of protein peaks resolved by RP-HPLC indicated that many of the eluted peaks contained more than one protein species. Chromatographic protein patterns obtained for Neepawa wheat grown at different locations and in different years were qualitatively the same.Key words: Protein, high-performance liquid chromatography, wheat

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