scholarly journals Increased DNA and/or RNA content of synovial fluid cells in rheumatoid arthritis: a flow-cytometry study.

1984 ◽  
Vol 43 (2) ◽  
pp. 222-227 ◽  
Author(s):  
B Bonvoisin ◽  
G Cordier ◽  
J P Revillard ◽  
E Lejeune ◽  
M Bouvier
Keyword(s):  
Rheumatoid Arthritis ◽  
Flow Cytometry ◽  
Synovial Fluid ◽  
Flow Cytometry Study ◽  
Rna Content ◽  
Synovial Fluid Cells

scholarly journals Biologically active thrombomodulin is synthesized by adherent synovial fluid cells and is elevated in synovial fluid of patients with rheumatoid arthritis

Blood ◽  
1993 ◽  
Vol 81 (3) ◽  
pp. 726-733 ◽  
Author(s):  
EM Conway ◽  
B Nowakowski
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Protein C ◽  
Inflammatory Process ◽  
Biologically Active ◽  
Northern Analysis ◽  
Synovial Lining ◽  
Local Synthesis ◽  
Synovial Lining Cells ◽  
Synovial Fluid Cells

Abstract Thrombomodulin (TM) is a transmembrane glycoprotein that interacts with thrombin, thereby serving as a cofactor in the activation of protein C, a major physiologically relevant natural anticoagulant. Although initially described as a vascular endothelial cell receptor, TM has also been reported to be synthesized by several cells, including megakaryocytes, platelets, monocytes, neutrophils (PMN), mesothelial cells, and synovial lining cells. A prominent feature of rheumatoid arthritis (RA) is infiltration of PMN into the joint space. To determine whether TM might play a role in the inflammatory process, we examined synovial fluid for the presence of TM in 10 patients with RA and five patients with osteoarthritis (OA). We determined that the mean synovial fluid and plasma TM levels in the OA group were 23.5 ng/mL and 24.2 ng/mL, respectively, whereas those with RA had a significantly elevated mean synovial fluid TM level of 136.2 ng/mL as compared with the plasma TM concentration of 43.9 ng/mL (P < .05). Synovial fluid TM levels did not correlate with PMN counts (r = .261). Purified TM from synovial fluid was identical in molecular weight to plasma-derived TM and was biologically functional with respect to protein C cofactor activity. Using direct immunofluorescence, we determined that adherent cultured synovial fluid cells that are not monocytoid in origin express surface and cytoplasmic TM, thereby providing an alternative source of the protein. Biologic activity of the cell-surface TM was confirmed by acceleration of thrombin-dependent protein C activation. Northern analysis of RNA extracted from the cultured cells indicated that TM messenger RNA was present, suggesting local synthesis. Our results indicate that in RA-associated synovial effusions, biologically active TM is increased, the source of which may be from plasma, PMN, and/or synovial lining cells. TM may play a regulatory role either in fibrin deposition in the inflamed joint and/or in the progression of the inflammatory process.

Trisomy 7 in synovial fluid cells of patients with rheumatoid arthritis

2004 ◽  
Vol 25 (8) ◽  
pp. 571-575 ◽  
Author(s):  
Funda Tascioglu ◽  
Beyhan Durak ◽  
Cengiz Oner ◽  
Sevilhan Artan
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Trisomy 7 ◽  
Synovial Fluid Cells

Ultrastructural characteristics of synovial fluid cells in rheumatoid arthritis and osteoarthritis

2014 ◽  
Vol 42 (1) ◽  
pp. 21
Author(s):  
AmalA Abd-El-Hafez ◽  
Mervat Esmail ◽  
Ali El-Deeb ◽  
Rehab Al Sernagawy
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Ultrastructural Characteristics ◽  
Synovial Fluid Cells

scholarly journals AB0094 FUNCTIONAL TREG CELLS MAY BE CONVERTED INTO T EFFECTOR PHENOTYPE ON EXPOSURE TO INFLAMMATORY MILIEU IN RHEUMATOID ARTHRITIS (RA) SYNOVIAL FLUID: IN-VITRO STUDY

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1346.1-1347
Author(s):  
V. Kommoju ◽  
C. Kavadichanda ◽  
V. Negi
Keyword(s):  
Rheumatoid Arthritis ◽  
Flow Cytometry ◽  
Synovial Fluid ◽  
Cell Surface ◽  
Inflammatory Cytokines ◽  
Peripheral Blood ◽  
Treg Cells ◽  
Cell Surface Expression ◽  
Inflammatory Milieu

Background:The debate on functional versus numerical difference in T regulatory cell population among patients with Rheumatoid arthritis (RA) is not clear. Tregs expressing Inflammatory subset phenotype markers, such as Th1(cxcr3, Tbet) and Th17(ccr6,Rorϒ) are reported. (1) Though the reported numbers of synovial fluid tregs are higher in RA, (2) the fate of Tregs on entering the synovial inflammatory milieu from peripheral blood (PB) has not yet been investigated.Objectives:To compare Treg frequencies in PB and synovial fluid between osteoarthritis (OA) and RATo compare cytokine levels in PB and SF between OA and RATo study the effect of autologous synovial fluid on RA and OA Treg isolated form peripheral bloodMethods:The Peripheral Blood (PB) and synovial fluid (SF) of RA (n=80) and OA (n=30) patients were analyzed for CD4+T-cell subset frequencies and phenotypes by flow cytometry. Cytokine concentrations in plasma and SF were measured by cytometric bead array. Tregs from 5 RA-PB and 5 OA-PB were isolated and cultured in autologous synovial fluid for 24 hrs. Phenotypic expression of Th1 and Th17 chemokines on the cell surface were analyzed by flow cytometry and expression levels of T-bet, RORγ and FOXP3 in those Treg cells were measured with quantitative real-time PCR (RT-qPCR).Results:The PB and SF frequencies of Th1, Th17 and Tregs are shown in Table 1. The pro-inflammatory cytokines were high in the plasma and SF of RA but the anti-Inflammatory cytokines were similar (Fig 1.A&B). Treg cells were isolated from RA and OA PB and cultured in autologous SF for 24 hrs. RA Treg showed increased cell surface expression of CXCR3+ and CCR6+ (Fig 1C) and there was no difference in OA Treg. Gene expression studies showed an increased expression of T-bet, RORγ and decreased expression of Foxp3 in RA Tregs while there was no difference in OA Tregs before and after in-vitro culture (Fig 1D).Table 1.Tcell subsets in Rheumatoid Arthritis and Osteoarthritis.CD4 subtypeRAOAPBSFPBSFN=80N=30N=30N=30Th126.65 ± 5.5934.99 ± 1.3025.97 ± 7.2426.45 ± 1.87*Th25.19 ± 1.916.36 ± 1.735.24 ± 2.151.44 ± 0.10*Th1714.05 ± 3.2921.18 ± 2.0410.81 ± 2.78*2.45 ± 0.23*Treg10.68 ± 2.4712.53 ± 2.1011.162.9513.04 ± 2.57*P<0.05Conclusion:Tregs in RA may be converted to Th1 and Th17 phenotype on exposure to inflammatory cytokine in the synovial fluid, thus losing their regulatory functions. Understanding factors influencing stability of Treg cells may help improve future therapeutics.References:[1]Jinlin Miao & Ping Zhu. Functional Defects of Treg Cells: New Targets in Rheumatic Diseases, Including Ankylosing Spondylitis. Current Rheumatology Reports 2018; 20: 30[2]Penatti et al. Differences in serum and synovial CD4+ T cells and cytokine profiles to stratify patients with inflammatory osteoarthritis and rheumatoid arthritis. Arthritis Research & Therapy.2017; 19:103Acknowledgments:Department of Science and technology, India for the research grant.Disclosure of Interests:None declared

Autometallographic Demonstration of Gold Uptake in Cultured Synovial Fluid Cells from Patients with Rheumatoid Arthritis

1989 ◽  
Vol 18 (3) ◽  
pp. 161-164 ◽  
Author(s):  
E. Jacobsen ◽  
A. Andreasen ◽  
H. Graudal ◽  
G. Danscher
Keyword(s):  
Rheumatoid Arthritis ◽  
Synovial Fluid ◽  
Synovial Fluid Cells
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