Background: Targeted self-management programmes may improve health and increase physical activity in people with multimorbidity. Aim: Investigate the impact of a structured, theoretically-driven self-management group education programme on habitual physical activity levels in people with multimorbidity. Design: Individually randomised controlled trial with 12-month follow-up. Setting: Nine primary care practices within Leicestershire, UK. Methods: N=353 adults with multimorbidity (age 67.8±9 years, 161 male) were randomly assigned (1:1) to intervention (n=180) or control (n=173) groups. Intervention participants were invited to attend four group-based self-management sessions, centred primarily on increasing physical activity. They also received motivational text message support. The primary outcome measure was change from baseline in device-measured (GENEActiv wrist-worn accelerometer) overall volume of daily physical activity at 12 months. Results: At baseline, the total sample was achieving 22mins/day of moderate-to-vigorous intensity physical activity. At 12 months a reduction in daily average physical activity was seen in the intervention group relative to control participants in the complete-case analysis (-0.80 mg; 95% CI: -1.57, -0.03; p=0.04) (primary outcome data available for 71.1% and 79.2% of intervention and control groups respectively). Similar reductions were seen in time spent in moderate-to-vigorous physical activity (-3.86 mins/day; 95% CI:-6.70, -1.03; p=0.01) and time spent at an intensity equivalent to a slow walk (-4.66 mins/day; 95% CI: -8.82, -0.51; p=0.028). Conclusions: The self-management programme elicited a slight reduction in physical activity levels in people with multimorbidity. Future studies should identify and target subgroups of those with multimorbidity at greatest need for physical activity promotion.