CUCUMBER NECROSIS VIRUS

1959 ◽  
Vol 37 (5) ◽  
pp. 913-925 ◽  
Author(s):  
Colin D. McKeen
Keyword(s):  
Thermal Inactivation ◽  
French Bean ◽  
Upward Movement ◽  
Thermal Coefficient ◽  
Necrosis Virus ◽  
Greenhouse Production ◽  
Wide Variability ◽  
Invasive Capacity ◽  
Cucumber Necrosis Virus ◽  
Thermal Inactivation Point

Cucumber necrosis virus (CNV) has been isolated several times during the last 7 years from cucumbers grown under glass in southwestern Ontario. Cucumber is the only host known to be systemically infected by the virus. Although CNV exhibits many properties of viruses included in the tobacco necrosis (TNV) group, it is considered to be sufficiently different to warrant the above distinctive designation.CNV possesses a small thermal coefficient, a thermal inactivation point between 75 and 80 °C, and a dilution end point between 10−4 and 10−5.During the short-day season of greenhouse production of cucumbers the virus causes severe foliar symptoms. Serious stunting of growth occurs and infected plants usually die 6 weeks to 2 months after the virus becomes systemic. In natural infections CNV enters by way of the roots, and subsequently may invade the aerial organs. Wide variability in the upward movement of the virus from inoculated cotyledons and roots of cucumber plants of the same age is noted.In inoculated unifoliate leaves of cowpea and French bean CNV displays a lower invasive capacity than TNV. With serial transfers of juice from cowpea leaves, inoculated initially with both viruses, TNV soon predominates.

PROPERTIES OF A STRAIN OF CUCUMBER-MOSAIC VIRUS ISOLATED FROM PRUNUS HOSTS

1957 ◽  
Vol 35 (5) ◽  
pp. 763-771 ◽  
Author(s):  
R. S. Willison ◽  
M. Weintraub
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Thermal Inactivation ◽  
Aphis Gossypii ◽  
Alfalfa Mosaic Virus ◽  
Datura Stramonium ◽  
Stone Fruit ◽  
Thermal Coefficient ◽  
Nicotiana Glutinosa ◽  
Thermal Inactivation Point

Some of the stone-fruit viruses that are transmissible only to cucumber and other cucurbits have occasionally been accompanied by a second virus that can be isolated by transfer to tobacco. This virus, herein called CMVP, appears to be latent in Prunus hosts, but induces symptoms in bean, cowpea, cucumber, Datura stramonium, Nicotiana glutinosa, petunia, tobacco, spinach, sugar beet, Swiss chard, and zinnia. CMVP has a small thermal coefficient, a thermal inactivation point between 65° and 70 °C, and a dilution end point between 10−3 and 10−4. It remains infective in expressed sap up to 96 hours at room temperature and for more than 6 days under refrigeration. It can be transmitted between cucumber and tobacco by Aphis gossypii and Myzus persicae, in which it is nonpersistent. Spherical "virus particles" associated with CMVP are about 35 mμ in diameter. Tobacco plants infected with this virus are partially protected against cucumber mosaic virus but not against tobacco ring spot virus.In symptom expression and in some of its properties, CMVP resembles both alfalfa mosaic virus and cucumber mosaic virus. Its particle size and immunological reaction suggest that it is an atypical strain of the latter. It is considered not to be implicated in the etiology of cherry yellows and related stone-fruit viroses.

Host range and properties of tobacco stunt virus

1975 ◽  
Vol 53 (21) ◽  
pp. 2425-2434 ◽  
Author(s):  
C. Hiruki
Keyword(s):  
Host Range ◽  
Comparative Studies ◽  
Phosphate Buffer ◽  
Host Plants ◽  
Thermal Inactivation ◽  
Cross Protection ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Serological Reaction ◽  
Thermal Inactivation Point

Tobacco stunt virus (TSV) was mechanically transmitted to 41 species in 9 families: i.e., Aizoaceae, Amaranthaceae, Apocynaceae, Caryophyllaceae, Chenopodiaceae, Cucurbitaceae, Leguminosae, Pedaliaceae, and Solanaceae. TSV remained infective for 60 h in 0.001 M 1-phenylthiosemicarbazide (1-PTC) in 0.01 M phosphate buffer, pH 6.8, but was infective only immediately after extraction in phosphate buffer. TSV in 1-PTC-phosphate buffer had a thermal inactivation point between 75 and 80 °C and a dilution end point between 10−2 and 10−3. Comparative studies made on reaction of host plants, serological reaction, and cross protection indicate that TSV is unrelated to a California isolate of tobacco necrosis virus.

scholarly journals A Survey of Resistance to Tomato bushy stunt virus in the Genus Nicotiana Reveals That the Hypersensitive Response Is Triggered by One of Three Different Viral Proteins

2013 ◽  
Vol 26 (2) ◽  
pp. 240-248 ◽  
Author(s):  
Carlos A. Angel ◽  
James E. Schoelz
Keyword(s):  
Coat Protein ◽  
Hypersensitive Response ◽  
Rna Silencing ◽  
Mutational Analysis ◽  
Movement Protein ◽  
Viral Proteins ◽  
Tomato Bushy Stunt Virus ◽  
Necrosis Virus ◽  
Nicotiana Glutinosa ◽  
Cucumber Necrosis Virus

In this study, we screened 22 Nicotiana spp. for resistance to the tombusviruses Tomato bushy stunt virus (TBSV), Cucumber necrosis virus, and Cymbidium ringspot virus. Eighteen species were resistant, and resistance was manifested in at least two different categories. In all, 13 species responded with a hypersensitive response (HR)-type resistance, whereas another five were resistant but either had no visible response or responded with chlorotic lesions rather than necrotic lesions. Three different TBSV proteins were found to trigger HR in Nicotiana spp. in an agroinfiltration assay. The most common avirulence (avr) determinant was the TBSV coat protein P41, a protein that had not been previously recognized as an avr determinant. A mutational analysis confirmed that the coat protein rather than the viral RNA sequence was responsible for triggering HR, and it triggered HR in six species in the Alatae section. The TBSV P22 movement protein triggered HR in two species in section Undulatae (Nicotiana glutinosa and N. edwardsonii) and one species in section Alatae (N. forgetiana). The TBSV P19 RNA silencing suppressor protein triggered HR in sections Sylvestres (N. sylvestris), Nicotiana (N. tabacum), and Alatae (N. bonariensis). In general, Nicotiana spp. were capable of recognizing only one tombusvirus avirulence determinant, with the exceptions of N. bonariensis and N. forgetiana, which were each able to recognize P41, as well as P19 and P22, respectively. Agroinfiltration failed to detect the TBSV avr determinants responsible for triggering HR in N. arentsii, N. undulata, and N. rustica. This study illustrates the breadth and variety of resistance responses to tombusviruses that exists in the Nicotiana genus.

Involvement of the Cucumber Necrosis Virus Coat Protein in the Specificity of Fungus Transmission by Olpidium bornovanus

Virology ◽  
1994 ◽  
Vol 204 (2) ◽  
pp. 840-842 ◽  
Author(s):  
M.A. McLean ◽  
R.N. Campbell ◽  
R.I. Hamilton ◽  
D.M. Rochon
Keyword(s):  
Coat Protein ◽  
Necrosis Virus ◽  
Virus Coat Protein ◽  
Olpidium Bornovanus ◽  
Cucumber Necrosis Virus ◽  
Virus Coat

scholarly journals Thermal Inactivation of Infectious Pancreatic Necrosis Virus in a Peptone-Salt Medium Mimicking the Water-Soluble Phase of Hydrolyzed Fish By-Products

2012 ◽  
Vol 78 (7) ◽  
pp. 2446-2448 ◽  
Author(s):  
Halvor Nygaard ◽  
Ingebjørg Modahl ◽  
Mette Myrmel
Keyword(s):  
Pancreatic Necrosis ◽  
Thermal Inactivation ◽  
Infectious Pancreatic Necrosis Virus ◽  
Water Soluble ◽  
Heat Inactivation ◽  
Necrosis Virus ◽  
Soluble Phase ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
By Products

ABSTRACTInfectious pancreatic necrosis virus (IPNV) (serotype Sp) was exposed to temperatures between 60 and 90°C in a medium mimicking the water-soluble phase of hydrolyzed fish by-products.Dvalues ranged from 290 to 0.5 min, and thezvalue was approximately 9.8°C. Addition of formic acid to create a pH 4 medium did not enhance heat inactivation. Predicted inactivation effects at different temperature-time combinations are provided.

Determination of the Thermal Inactivation Point

1998 ◽  
pp. 102-104 ◽  
Author(s):  
Jeanne Dijkstra ◽  
Cees P. de Jager
Keyword(s):  
Thermal Inactivation ◽  
Thermal Inactivation Point

Stipple streak disease of French bean caused by a tobacco necrosis virus in Queensland

1968 ◽  
Vol 19 (5) ◽  
pp. 731 ◽  
Author(s):  
GM Behncken
Keyword(s):  
French Bean ◽  
Tobacco Necrosis Virus ◽  
Serological Evidence ◽  
Necrosis Virus ◽  
Leaf Vein ◽  
Satellite Virus ◽  
Vein Necrosis ◽  
Stem Necrosis ◽  
Olpidium Brassicae

A disease of beans in the Nambour district of Queensland has been shown to be stipple streak disease caused by a tobacco necrosis virus. Symptoms include leaf vein necrosis, stem necrosis, and occasionally necrotic lesions on the pods. In glasshouse tests symptoms developed more rapidly, and were more severe, at temperatures of 80–88°F than at 62–70°. The virus was readily transmitted by zoospores of a lettuce isolate of the fungus Olpidium brassicae (Wor.) Dang. Serological evidence is presented which indicates that the virus is an "A" serotype strain of tobacco necrosis virus. No evidence for the presence of an associated satellite virus was found.

Vergleich zwischen Eigenschaften des Echten Ackerbohnenmosaik-Virus und des broad bean mottle-Virus

1960 ◽  
Vol 15 (7) ◽  
pp. 444-447 ◽  
Author(s):  
C. Wetter ◽  
H. L. Paul ◽  
J. Brandes ◽  
L. Quantz
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Thermal Inactivation ◽  
Broad Bean ◽  
Seed Transmission ◽  
Mottle Virus ◽  
Nucleic Acid Content ◽  
Thermal Inactivation Point ◽  
Broad Bean Mottle Virus ◽  
Point Seed

Broad bean true mosaic virus (EAMV) has been studied in comparison with broad bean mottle virus (BBMV), after both viruses had been purified by the same procedure. Spectrophotometric and/or chemical determinations revealed a nucleic acid content of 32% for EAMV and 23% for BBMV. Serologically, the two viruses are unrelated since in reciprocal testings they react with their homologous antisera only. The diameter of of the particles has been determined to 25 mμ for EAMV and 20 mμ for BBMV. Further differences include thermal inactivation point, seed transmission, host range, and symptomatology.There are no indications for a relationship among both viruses as suggested in the list of Common names (Review of Applied Mycology, Vol. 35, Suppl.).

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