Host range and properties of tobacco stunt virus

1975 ◽  
Vol 53 (21) ◽  
pp. 2425-2434 ◽  
Author(s):  
C. Hiruki
Keyword(s):  
Host Range ◽  
Comparative Studies ◽  
Phosphate Buffer ◽  
Host Plants ◽  
Thermal Inactivation ◽  
Cross Protection ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Serological Reaction ◽  
Thermal Inactivation Point

Tobacco stunt virus (TSV) was mechanically transmitted to 41 species in 9 families: i.e., Aizoaceae, Amaranthaceae, Apocynaceae, Caryophyllaceae, Chenopodiaceae, Cucurbitaceae, Leguminosae, Pedaliaceae, and Solanaceae. TSV remained infective for 60 h in 0.001 M 1-phenylthiosemicarbazide (1-PTC) in 0.01 M phosphate buffer, pH 6.8, but was infective only immediately after extraction in phosphate buffer. TSV in 1-PTC-phosphate buffer had a thermal inactivation point between 75 and 80 °C and a dilution end point between 10−2 and 10−3. Comparative studies made on reaction of host plants, serological reaction, and cross protection indicate that TSV is unrelated to a California isolate of tobacco necrosis virus.

CUCUMBER NECROSIS VIRUS

1959 ◽  
Vol 37 (5) ◽  
pp. 913-925 ◽  
Author(s):  
Colin D. McKeen
Keyword(s):  
Thermal Inactivation ◽  
French Bean ◽  
Upward Movement ◽  
Thermal Coefficient ◽  
Necrosis Virus ◽  
Greenhouse Production ◽  
Wide Variability ◽  
Invasive Capacity ◽  
Cucumber Necrosis Virus ◽  
Thermal Inactivation Point

Cucumber necrosis virus (CNV) has been isolated several times during the last 7 years from cucumbers grown under glass in southwestern Ontario. Cucumber is the only host known to be systemically infected by the virus. Although CNV exhibits many properties of viruses included in the tobacco necrosis (TNV) group, it is considered to be sufficiently different to warrant the above distinctive designation.CNV possesses a small thermal coefficient, a thermal inactivation point between 75 and 80 °C, and a dilution end point between 10−4 and 10−5.During the short-day season of greenhouse production of cucumbers the virus causes severe foliar symptoms. Serious stunting of growth occurs and infected plants usually die 6 weeks to 2 months after the virus becomes systemic. In natural infections CNV enters by way of the roots, and subsequently may invade the aerial organs. Wide variability in the upward movement of the virus from inoculated cotyledons and roots of cucumber plants of the same age is noted.In inoculated unifoliate leaves of cowpea and French bean CNV displays a lower invasive capacity than TNV. With serial transfers of juice from cowpea leaves, inoculated initially with both viruses, TNV soon predominates.

scholarly journals First Complete Genome Sequence of Tobacco necrosis virus D Isolated from Soybean and from North America

2017 ◽  
Vol 5 (32) ◽  
Author(s):  
Gustavo A. Díaz-Cruz ◽  
Charlotte M. Smith ◽  
Kiana F. Wiebe ◽  
Bryan J. Cassone
Keyword(s):  
North America ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Host Plants ◽  
Tobacco Necrosis Virus ◽  
Soybean Plant ◽  
Necrosis Virus ◽  
Geographic Regions

ABSTRACT We present the first complete genome sequence of the tombusvirus Tobacco necrosis virus D (TNV-D) from North America, obtained from an infected soybean plant. Compared with the three other TNV-D genomes isolated from different geographic regions and host plants, its nucleotide identities were between 83% and 93%.

Centrosema mosaic: A new virus disease of Crotalaria SPP. in Papua and New Guinea

1962 ◽  
Vol 13 (2) ◽  
pp. 220 ◽  
Author(s):  
Velsen RJ Van ◽  
NC Crowley
Keyword(s):  
Host Range ◽  
Thermal Inactivation ◽  
Virus Disease ◽  
Aphis Gossypii ◽  
Modes Of Transmission ◽  
Cuscuta Campestris ◽  
Aphis Craccivora Koch ◽  
Calopogonium Mucunoides ◽  
Thermal Inactivation Point

A study has been made of an undescribed virus disease which has been called Centrosema mosaic. Observations on its occurrence and symptoms, and data on its host range and modes of transmission are presented. The virus occurs naturally in the field on Crotalaria anagyroides H.U.K., C. goreensis Guill. & Perr., C. retusa L., C. mucronata Desv., Calopogonium mucunoides Desv., Centrosema pubescens Benth., and Desmodium distortum (Aubl.) Macbride. The host range appears to be restricted to the Leguminosae, and mainly to Crotalaria spp. The virus is mechanically transmissible, has a thermal inactivation point between 55 and 58°C, a dilution end-point of 1 in 2000, and a longevity in vitro of less than 6 hr. It is transmitted in a non-persistent manner by the aphids Aphis gossypii Glover, Aphis craccivora (Koch), Myzus persicae (Sulz.), and Brachycaudus helichrysi (Kalt.) var. warei (Theob.) and by two species of plant bugs of the genus Nysius. It is readily transmitted by Cuscuta campestris Yuncker, but not by seed or soil.

Thermal inactivation of tobacco necrosis virus

Virology ◽  
1963 ◽  
Vol 20 (3) ◽  
pp. 490-497 ◽  
Author(s):  
P. Babos ◽  
B. Kassanis
Keyword(s):  
Thermal Inactivation ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

scholarly journals DETERMINATION OF PROPERTIES OF A STRAIN OF TOBAMOVIRUS FROM MUCUNA PRURIENS

2020 ◽  
Vol 18 (1) ◽  
pp. 22-31
Author(s):  
O. O. ODEDARA ◽  
A. C. ODEBODE ◽  
J. A. HUGHES
Keyword(s):  
Host Range ◽  
Thermal Inactivation ◽  
Biological Properties ◽  
Mucuna Pruriens ◽  
Infected Leaf ◽  
Plant Host ◽  
End Point ◽  
The Family ◽  
Thermal Inactivation Point

A virus-infected leaf of Mucuna pruriens was collected and tested for an incidence of a virus through biological properties using various plant host range inoculation tests, longevity-in-vitro, dilution-end-point, thermal-inactivation point and electron microscopy. Results of host range inoculation tests showed the following plant species to be susceptible to the virus; Nicotiana tabacum, N. rustica, N. benthamiana, N. glutinosa, N. occidentalis, N. Sylvesris (Speg and Comes) and others. No plant in the family Convolvulacaee, Cucurbitaceae was found to be infected with the virus.  However Chenopodium quinoa and C. murale in the family Chenopodiaceae were infected with symptoms of mottling and necrotic lesions.  In the family Fabaceae, hybrid cowpea line TVu 76, IT84S – 2114 were infected with mosaic, leaf crinkling and severe leaf curl respectively. The virus had a dilution-end-point of 10-6-10-7, thermal-inactivation-point of 95°C in crude sap of TVu 76. The virus is of agricultural importance because of its incidence on seed coats of legumes which are freely exchanged between agricultural stakeholders.      

scholarly journals Some Properties of a Virus From Galinsoga Parviflora

1970 ◽  
Vol 23 (2) ◽  
pp. 497 ◽  
Author(s):  
GM Behncken
Keyword(s):  
Mung Bean ◽  
Tobacco Necrosis Virus ◽  
Weed Species ◽  
Necrosis Virus ◽  
Serological Reaction ◽  
French Beans ◽  
South Eastern

During an investigation of stipple streak disease of French beans near Nambour in south-eastern Queensland (Behncken 1968), the roots of a number of weed species were indexed for tobacco necrosis virus (TNV). A virus was regularly isolated from the roots of Galinsoga parvijlora Cav., an annual commonly called potato weed. It was distinguished from TNV on the basis of differences in host reactions, absence of any serological reaction with TNV antisera, and its failure to be transmitted to the roots of seedlings of mung bean (Phaseolu8 aureU8 Roxb.) by zoospores of the fungus Olpidium bra88icae (Wor.) Dang.

scholarly journals Biological and Physico-chemical Properties of Causal Agent of Virus Disease of Coccinia barteri (Hook. f.) Keay in Calabar, Cross River State

2020 ◽  
pp. 1-6
Author(s):  
V. E. Uyoh ◽  
O. T. Umoh ◽  
A. T. Toby ◽  
O. M. Umoden
Keyword(s):  
Host Range ◽  
Thermal Inactivation ◽  
Virus Disease ◽  
Chemical Properties ◽  
Causal Agent ◽  
Diagnostic Tools ◽  
Virus Concentration ◽  
Physico Chemical ◽  
Thermal Inactivation Point

The physico-chemical properties of the causal agent of virus disease of Coccinia barteri (Hook. f.) Keay were studied. The virus causing the disease was characterized using diagnostic tools such as host range, longevity in vitro, thermal inactivation point, dilution endpoint and aphid transmission. The virus was mechanically transmitted from the natural host (C. barteri) to the healthy test plants in the green house. In the biological properties, the virus was successfully transmitted by Aphis spiraecola (obtained from Chromolaena odorata (L.) R. M. King & H. Rob.) from infected Cucumeropsis mannii Naudin to a healthy C. mannii in a non-persistent manner and had a narrow host range limited to the family Cucurbitaceae. In the physico-chemical properties based on crude sap with an unknown virus concentration, beyond which infectivity was lost. It was readily inactivated by heating to 35 – 65°C for 10 minutes in determination of thermal inactivation point. The virus had a longevity in vitro of between 4 – 5 days beyond which it was non-infectious. Symptoms induced by the virus were leaf cupping, mottle chlorosis, blisters, stunted growth, rugosity, leaf malformation and mosaic patterns.

scholarly journals Summer Cosmos – A Host of Cucumber mosaic virus

1970 ◽  
pp. 84-93
Author(s):  
MS Parvin ◽  
AM Akanda ◽  
AHMA Rahman
Keyword(s):  
Host Range ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Thermal Inactivation ◽  
Virus Disease ◽  
Local Lesion ◽  
Severe Stunting ◽  
End Point ◽  
Thermal Inactivation Point

In order to identify the cause of virus disease-like symptoms developed naturally in Summer cosmos (Cosmos sulphureus) plants at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur campus, a study was conducted during March 2004 to August 2005. The natural symptoms in Summer cosmos were consisted of mosaic, yellowing, shoe-string and leaf curling along with severe stunting of the infected plants. The ailments were found to be sap transmissible. Gomphrena globosa and Chenopodium amaranticolor were found to be good local lesion hosts producing chlorotic local lesion in the inoculated plants. The virus isolates obtained from the infected G. globosa plant had wide host range including Amaranthaceae, Chenopodiaceae, Compositae, Cucurbitaceae, Ligominosae and Solanaceae. The dilution end point, thermal inactivation point and longevity in vitro were determined as 10-6, 65°C and 10 days, respectively. The host range test, dilution end point, thermal inactivation point and longevity in vitro suggested that the virus was identical to Cucumber mosaic virus (CMV). Double Antibody Sandwich Enzyme-Linked Immuno-Sorbent Assay (DAS-ELISA) detected the virus as CMV. The results of the study revealed that the virus disease-like symptoms naturally manifested in summer cosmos plants was identified as CMV. Key words: Summer cosmos, CMV, virus identification. DOI = 10.3329/jard.v5i1.1463 J Agric Rural Dev 5(1&2), 84-93, June 2007

SPECIES, HOST RANGE, AND IDENTIFICATION KEY OF WHITEFLIES OF BOGOR AND SURROUNDING AREA

2019 ◽  
Vol 18 (2) ◽  
pp. 127
Author(s):  
Purnama Hidayat ◽  
Denny Bintoro ◽  
Lia Nurulalia ◽  
Muhammad Basri
Keyword(s):  
Host Range ◽  
Host Plants ◽  
Ornamental Plants ◽  
Identification Key ◽  
Agricultural Crops ◽  
Surrounding Area ◽  
Aleurodicus Dispersus ◽  
Agricultural Plants ◽  
Surrounding Areas ◽  
Forest Plants

Species identification, host range, and identification key of whiteflies of Bogor and surrounding area. Whitefly (Hemiptera: Aleyrodidae) is a group of insects that are small, white, soft-bodied, and easily found on various agricultural crops. Whitefly is a phytophagous insect; some species are important pests in agricultural crops that can cause direct damage and can become vectors of viral diseases. The last few years the damage caused by whitefly in Indonesia has increased. Unfortunately, information about their species and host plants in Indonesia, including in Bogor, is still limited. Kalshoven, in his book entitled Pest of Crops in Indonesia, published in the 1980s reported that there were 9 species of whitefly in Indonesia. The information on the book should be reconfirmed. Therefore, this study was conducted to determine whitefly species and its host plants in Bogor and its surroundings. Whiteflies is identified based on the ‘puparia’ (the last instar of the nymph) collected from various agricultural plants, ornamental plants, weeds, and forest plants. A total of 35 species of whiteflies were collected from 74 species and 29 families of plants. The collwcted whiteflies consist of four species belong to Subfamily Aleurodicinae and 31 species of Subfamily Aleyrodinae. The most often found whitefly species were Aleurodicus dispersus, A. dugesii, and Bemisia tabaci. A dichotomous identification key of whiteflies was completed based on morphological character of 35 collected species. The number of whitefly species in Bogor and surrounding areas were far exceeded the number of species reported previously by Kalshoven from all regions in Indonesia.

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