The effect of lettuce seed extracts on lettuce seed germination

1975 ◽  
Vol 53 (7) ◽  
pp. 593-599 ◽  
Author(s):  
Henry L. Speer ◽  
Dorothy Tupper
Keyword(s):  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Seed Germination ◽  
Seed Coat ◽  
Gas Liquid Chromatography ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Grand Rapids ◽  
Lettuce Seed Germination ◽  
Seed Extracts

Lettuce seeds (Lactuca sativa var. Grand Rapids) were found to contain inhibitory substances, one of which is probably abscisic acid. Extracts from seeds were characterized by gas–liquid chromatography, and peaks coincident with abscisic acid were found.The germination water surrounding seeds made secondarily dormant was subjected to gas–liquid chromatography and was also found to contain peaks coincident with abscisic acid. It was also determined that the inhibitory substances are localized in the embryo but not in the endosperm or seed coat.

scholarly journals Preventing thermoinhibition in a thermosensitive lettuce genotype by seed imbibition at low temperature

2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Temperature Dependent ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lettuce Seed Germination ◽  
Dark Green

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.

Nitrate, abscisic acid and gibberellin interactions on the thermoinhibition of lettuce seed germination

2011 ◽  
Vol 66 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Tingting Dong ◽  
Jianhua Tong ◽  
Langtao Xiao ◽  
Hongyan Cheng ◽  
Songquan Song
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Lettuce Seed ◽  
Lettuce Seed Germination

scholarly journals RELATIONSHIP BETWEEN THE SEED COAT AND EMBRYO IN REGULATING THE EFFECT OF HEAT AND SALT STRESS ON LETTUCE SEED GERMINATION

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1156b-1156
Author(s):  
James Dunlap ◽  
Brian Scully ◽  
Dawn Reyes
Keyword(s):  
Seed Coat ◽  
Turgor Pressure ◽  
Physiological Mechanism ◽  
Nacl Stress ◽  
Lettuce Seed ◽  
Embryo Germination ◽  
Critical Range ◽  
Lettuce Seeds ◽  
Lettuce Seed Germination ◽  
Expansion Force

Poor germination of lettuce seeds exposed to heat and salinity is attributed to a reduction in the capacity for embryo expansion. Ethylene and kinetin are proposed to overcome these stresses by increasing the expansion force of the embryo which ruptures the seed coat barrier to growth. To better understand the physiological mechanism regulating thermodormancy in the embryo, germination was determined for intact and decoated seeds from thermosensitive and thermotolerant varieties subjected to a critical range of temperature and salt (NaCl) stress. Although more tolerant of stress, the response of decoated seeds to ACC and kinetin was similar to the response of intact seeds. No interaction between ACC and kinetin was detected in decoated seed except under the most severe stress and in the thermosensitive variety. Heat and salt tolerance appear to be governed by the same physiological mechanism. We propose that the seed coat plays no qualitative role in the expression of lettuce seed thermodormancy. The response occurs exclusively in the embryo and may result from an inability to generate sufficient turgor pressure at supraoptimal temperatures for cell expansion.

scholarly journals 402 Lettuce Seed Germination and Endo-mannanase Activity are Stimulated by Ethylene at High Temperature

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 513B-513 ◽  
Author(s):  
Warley M. Nascimento ◽  
Daniel J. Cantliffe ◽  
Donald J. Huber
Keyword(s):  
High Temperature ◽  
Seed Germination ◽  
Enzyme Regulation ◽  
Seed Priming ◽  
Lettuce Seed ◽  
Endosperm Cell ◽  
Lettuce Seeds ◽  
Mannanase Activity ◽  
Lettuce Seed Germination

Temperatures above 30 °C may delay or inhibit germination of most of commercial lettuce cultivars. Ethylene enhances lettuce seed germination at high temperatures. Enzyme-mediated degradation of endosperm cell walls appears to be a crucial factor for lettuce germination at high temperature. The galactomannan polysaccharides in lettuce endosperm cell wall are mobilized by endomannanase. The role of endo-mannanase during germination of lettuce seeds at high temperature (35 °C) and the possible role of etlene in enzyme regulation were investigated. Seeds of thermotolerant (`Everglades'-EVE) and thermosensitive (`Dark Green Boston'-DGB) lettuce genotypes were incubated at 20 and 35 °C in water, 10 mM of 1-aminocyclopropane-1-carboxylic acid (ACC), or 20 mM of silver thiosulphate (STS). Also, seeds were primed in an aerated solution of polyethylene glycol (PEG), or PEG+ACC, or PEG+STS. Untreated seeds germinated 100% at 20 °C. At 35 °C, EVE germinated 100%, whereas DGB germinated only 33%. Seed priming or adding ACC during imbibition increased germination of DGB to 100% at 35 °C. Adding STS during imbibition led to a decrease in germination at 35%C in EVE and completely inhibited germination of DGB. Priming with STS led to reduced germination at 35%C of both genotypes. EVE produced more ethylene than DGB during germination at high temperature. Providing ACC either during priming or during germination led to an increase in endo-mannanase activity, whereas STS inhibited mannanase activity. Higher endo-mannana activity was observed in EVE than DGB seeds. The results suggest that ethylene might overcome the inhibitory effect of high temperature in thermosensitive lettuce seeds via weakening of endosperm due to increased endo-mannanase activity.

Characterization of SAN 9789-Stimulated Lettuce (Lactuca sativa) Seed Germination

Weed Science ◽  
1985 ◽  
Vol 33 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Karl-Olof Widell ◽  
Christer Sundqvist ◽  
Hemming I. Virgin
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Lactuca Sativa ◽  
Red Light ◽  
Inhibitory Effect ◽  
Lettuce Seeds ◽  
Grand Rapids ◽  
Minor Degree ◽  
A Minor

Dark germination of light-requiring lettuce seeds (Lactuca sativaL. ‘Grand Rapids’) was stimulated by SAN 9789 [4-chloro-5-(methylamino-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone] and to a minor degree by BASF 13761 [4-chloro-5-methoxy-2-phenyl-3(2H)-pyridazinone] and BASF 44521 [4-chloro-5-methoxy-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], but not by’ pyrazon [5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone], SAN 9785 [4-chloro-5-(dimethylamino)-2-phenyl-3 (2H)-pyridazinone], SAN 9774 [5-amino-4-chloro-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], or SAN 6706 [4-chloro-5-(dimethylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone]. SAN 9789 stimulation was inhibited by cis-4-cyclohexene-1,2-dicarboximide (CHDC), and abscisic acid (ABA) at 1 × 10-4M. Red light nullified the inhibitory effect of CHDC (1 × 10-4M) but not the inhibitory effect of ABA (1 × 10-4M) on SAN 9789 stimulated germination. Gibberellic acid (GA3) and kinetin (6-furfurylaminopurine) increased the germination stimulatory effect of SAN 9789 in darkness. Temperatures above 25 C decreased the effect of SAN 9789, with a temperature of 35 C completely inhibiting germination. The inhibitory effect of CHDC was strongly decreased at temperatures below 20 C. SAN 9789-induced germination in darkness was always the same (25 to 26% units increase in germination) even though the red light-stimulated germination differed with the seed batch.

scholarly journals Allelopathic effect of Passiflora alata Curtis extracts on seed germination

2016 ◽  
Vol 7 (1) ◽  
pp. 129
Author(s):  
Allan Rocha de Freitas ◽  
José Carlos Lopes ◽  
Liana Hilda Golin Mengarda ◽  
Rafael Fonsêca Zanotti ◽  
Luan Peroni Venancio
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Passion Fruit ◽  
Allelopathic Effect ◽  
Lettuce Seed ◽  
Whole Grain ◽  
Lettuce Seeds ◽  
Embryo Extract ◽  
Plant Chemical ◽  
Lettuce Seed Germination

Many plant chemical compounds can interfere on seed germination and on the development of other plants. Thus, this research aimed to verify the effect of sweet passion fruit seeds (Passiflora alata Curtis) extracts, under lettuce seed germination and lettuce seedlings growing. The experiment was carried out in a controlled environment. The treatments were: T1-distilled water as a control; T2-embryo extract; T3- tegument extract; T4- whole grain extract; T5- soaking solution. Lettuce seed (Lactuca sativa L.) cv. Babá de Verão, was used as a bioindicator of the allelopathic effect. When the treatment of embryo extract of sweet passion fruit was applied, a lower germination percentage and lower speed emergence rate of lettuce seeds were observed. The largest number of abnormal seedlings was observed from seeds germinated in the embryo extract (T2) and the soaking solution (T5). The sweet passion fruit embryo extract discloses compounds that are able to inhibit the germination and can negatively influence the development of lettuce seedlings.

scholarly journals (207) Sensitivity of Two Lettuce Genotypes to Abscisic Acid during Germination

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1070A-1070
Author(s):  
Jiyoung Hong ◽  
Daniel Cantliffe
Keyword(s):  
Abscisic Acid ◽  
High Temperature ◽  
Seed Maturation ◽  
Lettuce Seed ◽  
Dark Condition ◽  
Optimal Range ◽  
Lettuce Seeds ◽  
Lettuce Seed Germination ◽  
Dark Green ◽  
Better Than

In many lettuce (Lactuca sativa L.) cultivars, temperatures above 30 °C can inhibit germination completely. Lettuce seeds imbibed at supraoptimal temperature for 72 hours or more will not germinate even when the temperature is returned to the optimal range. Sung et al. (1998) reported that thermosensitive `Dark Green Boston' and thermotolerant `Everglades' responded to temperature at seed maturation by being able to show greatly enhanced germination at 36 °C when seeds were matured at 30/20 °C. Abscisic acid (ABA) plays an important role relative to both dormancy and germination of many seeds and may contribute to lettuce seed thermodormancy. Therefore, sensitivity of `Everglades' and `Dark Green Boston' to ABA of seeds maturated at 30/20 °C and 20/10 °C in light and dark was determined. Seeds were germinated at 20 °C and 36 °C in light and dark. All seeds of `Dark Green Boston'and `Everglades' matured at 30/20 °C and 20/10 °C germinated similarly regardless of maturation temperature and light/dark condition at 20 °C. At 36 °C in dark, both genotypes matured at 30/20 °C germinated more than those matured at 20/10 °C. `Dark Green Boston' genotypes were more sensitive to ABA (0.5, 1, 10, 50, 100 μM) during germination at 36°C in dark. When ABA was added, seeds matured at 30/20 °C germinated better than those matured at 20/10 °C, regardless of genotype. At 36 °C in light, 40% of both genotypes were inhibited at 0.5 μM ABA. At 36 °C in dark, germination of all seeds but `Everglades' matured at 30/20 °C were completely inhibited at all ABA concentrations. Thus, seed maturation temperature has an influence on lettuce seed germination at high temperature and sensitivity to ABA.

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