scholarly journals Bright-field transmission imaging of carbon nanofibers on bulk substrate using conventional scanning electron microscopy

Author(s):  
Makoto Suzuki ◽  
Quoc Ngo ◽  
Hirohiko Kitsuki ◽  
Kristofer Gleason ◽  
Yusuke Ominami ◽  
...  
1978 ◽  
Vol 56 (18) ◽  
pp. 2303-2306 ◽  
Author(s):  
Richard A. Nolan

Resistant sporangia of Coelomomyces chironomi var. canadense Weiser and McCauley were examined by bright-field, phase-contrast, and scanning electron microscopy (SEM). The use of SEM facilitated the observation of previously undescribed complex furrows in the sporangial wall. The taxonomic criterion for varietal status is discussed, and the variety is elevated to species status. Coelomomyces canadense (Weiser and McCauley) Nolan stat. et comb. nov. is described with an emended Latin diagnosis.


2018 ◽  
Vol 27 (2) ◽  
pp. 146-153
Author(s):  
Anangela Ravena da Silva Leal ◽  
Simone Mousinho Freire ◽  
Marcelo Knoff ◽  
Delir Corrêa Gomes ◽  
Jeannie Nascimento dos Santos ◽  
...  

Abstract Atractis thapari Petter, 1966, an atractid nematode, was collected parasitizing the large intestine of tortoises of the species Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) (Testudinidae) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. Taxonomic identification was based on morphological and morphometrical features, and using bright-field and scanning electron microscopy. The present study adds new observations on the morphology of A. thapari, mainly relating to mouth papillae, the excretory pore, deirids, and male and female posterior ends. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) for A. thapari in these two tortoise species were: P = 100%, MI = 154,667, MA = 154,667, RI = 5,500-588,500 (Cc); P = 100%, MI = 93,639, MA = 93,639, RI = 1,000-224,500 (Cd). This report confirms the occurrence of A. thapari in Neotropical region, South America, Brazil, and extends its occurrences to a new host, the tortoise C. carbonarius. Adjustment of host management with the aim of improving hygiene and health conditions is suggested.


2014 ◽  
Vol 20 (1) ◽  
pp. 124-132 ◽  
Author(s):  
Binay Patel ◽  
Masashi Watanabe

AbstractScanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) is a convenient technique for soft materials characterization. Various specimen-holder geometries and detector arrangements have been used for bright-field (BF) STEM-in-SEM imaging. In this study, to further the characterization potential of STEM-IN-SEM, a new specimen holder has been developed to facilitate direct detection of BF signals and indirect detection of dark-field (DF) signals without the need for substantial instrument modification. DF imaging is conducted with the use of a gold (Au)-coated copper (Cu) plate attached to the specimen holder which directs highly scattered transmitted electrons to an off-axis yttrium-aluminum-garnet (YAG) detector. A hole in the copper plate allows for BF imaging with a transmission electron (TE) detector. The inclusion of an Au-coated Cu plate enhanced DF signal intensity. Experiments validating the acquisition of true DF signals revealed that atomic number (Z) contrast may be achieved for materials with large lattice spacing. However, materials with small lattice spacing still exhibit diffraction contrast effects in this approach. The calculated theoretical fine probe size is 1.8 nm. At 30 kV, in this indirect approach, DF spatial resolution is limited to 3.2 nm as confirmed experimentally.


Author(s):  
Naida Paola Araújo da Silva ◽  
Ana Rita de Lima ◽  
Érika Branco ◽  
Raul Henrique da Silva Pinheiro ◽  
Elane Guerreiro Giese

Abstract Among the Nematodes of Cerdocyon thous, the genus Pterygodermatites is characteristic for presenting cuticular projections along the body, being mentioned in different hosts in South America, although there are no records of its occurrence in the Amazon Biome. To enable further exploration in terms of their morphology and morphometry, the nematodes collected from cadavers of C. thous were fixed and observed using bright field microscopy and scanning electron microscopy, revealing characteristics compatible with P. (Multipectines) affinis, thus contributing more information about the geographic distribution of the parasite, as well as knowledge of the helminthological fauna of wild mammals in the Brazilian Amazon.


2004 ◽  
Vol 92 (3) ◽  
pp. 225-231 ◽  
Author(s):  
Delir Corr�a Gomes ◽  
Rodrigo Caldas Menezes ◽  
Joaquim J�lio Vicente ◽  
Reinalda Marisa Lanfredi ◽  
Roberto Magalh�es Pinto

2012 ◽  
Vol 18 (5) ◽  
pp. 1106-1111 ◽  
Author(s):  
Lei Wang ◽  
Jin Shao ◽  
Tingjun Ye ◽  
Lianfu Deng ◽  
Shijing Qiu

AbstractScrew implantation inevitably causes microdamage in surrounding bone. However, little is known about the detailed characteristics of microdamage in peri-screw bone. In this study, we developed a method to construct microdamage cast with methylmethacrylate (MMA) and observed the cast using scanning electron microscopy (SEM). In basic fuchsin stained bone sections observed by bright-field and fluorescence microscopy, diffuse damage, cross-hatched damage, and linear cracks were all presented in peri-screw bone. Using MMA casting/SEM method, we found numerous densely packed microcracks in the areas with diffuse damage. The osteocyte canaliculi and the microcracks consisting of diffuse damage had a similar diameter (or width), usually <0.5 μm, but their morphology was largely different. In the area with cross-hatched damage, the orientation of microcracks was similar to that in diffuse damage, but the number was significantly decreased. Many microcracks were thicker than 1 μm and associated with a rough surface. Large linear cracks (∼10 μm in diameter) occurred in different areas. Plenty of microcracks were present on the surface of some linear cracks. In conclusion, the MMA casting/SEM method can demonstrate the three-dimensional morphology of different types of microdamage, particularly the microcracks in diffuse damage, which are unable to be shown by light microscopy.


1978 ◽  
Vol 56 (11) ◽  
pp. 1372-1380 ◽  
Author(s):  
Rolf W. Mathewes

The pollen morphology of four species of Myriophyllum from northwestern North American populations is described from studies using bright-field, interference-contrast, and scanning electron microscopy. Results indicate that M. verticillatum, M. heterophyllum, and M. hippuroides can be determined to species on the basis of pollen morphology. Myriophyllum spicatum and M. exalbescens are not readily separated on pollen morphology, although two tentative differences are suggested for further testing. It is also concluded that M. verticillatum should be recognized as a species clearly distinct from M. spicatum.


2013 ◽  
Vol 275-277 ◽  
pp. 1798-1801
Author(s):  
Qiang Wu ◽  
Li Zhao

Uniform growth of WO3 with macroscopic structures was successfully achieved by using carbon nanofibers (CNFs) as template. Field emission scanning electron microscopy (FE-SEM), coupled with X-ray diffraction (XRD) analysis confirmed the template effect and the existence of WO3 immobilized on the macroscopic silica fiber.


2007 ◽  
Vol 26-28 ◽  
pp. 809-812
Author(s):  
Fei Li ◽  
Xiao Ping Zou ◽  
Jin Cheng ◽  
Hong Dan Zhang ◽  
Peng Fei Ren ◽  
...  

Catalytic grown carbon nanofibers have been obtained from decomposition of ethanol over copper plate. Ferric chloride of different concentration was employed as the catalyst precursor. Scanning electron microscopy has shown that different morphologies of carbon nanofibers can be obtained by using different concentration of catalyst precursor solution. The results from the Raman spectroscopic characterization have also demonstrated that the graphitization of carbon nanofibers can be tailored by control of the concentration of catalyst precursor solution. When the information from these characterization are combined with that of the associated morphologies of the carbon nanofibers, it is possible to synthesize perfect carbon nanofibers.


Sign in / Sign up

Export Citation Format

Share Document