Application of an osmium-maceration technique to observe plant–microbe interfaces of Italian ryegrass and crown rust fungi by scanning electron microscopy
Keyword(s):
The osmium-maceration technique (osmium–DMSO–osmium method) was used to observe intracellular structures of Italian ryegrass leaves infected with crown rust fungus (Puccinia coronata Corda var. coronata) by scanning electron microscopy. Prolonged maceration with dilute osmium removed the cytoplasmic matrix, and intracellular structures were disclosed in a bold relief. The three-dimensional configuration of nuclei, mitochondria, the endoplasmic reticulum, and other structures of host and parasite cells were visible. Key words: scanning electron microscopy (SEM), osmic maceration, intracellular structure, Puccinia coronata, Italian ryegrass.
1973 ◽
Vol 31
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pp. 430-431
2001 ◽
Vol 5
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pp. 92-93
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1971 ◽
Vol 29
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pp. 410-411
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1968 ◽
Vol 26
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pp. 164-165
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1994 ◽
Vol 42
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pp. 681-686
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2008 ◽
Vol 26
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2019 ◽
Vol 81
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pp. 454-465
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